FastQCFastQC Report
Mon 15 Aug 2016
SRR2921138_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921138_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1768102
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26590.15038725141422837No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24200.1368699317120845No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT23910.13522975484446031No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA23200.13121414940993223No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA93100.036.7195741
GGTATCA65050.031.917191
TATCAAC116650.029.1933522
GTAAGGT6850.028.7313444
TCAACGC119600.028.4234164
CAACGCA121250.028.0333465
ATCAACG122300.027.844683
AACGCAG124150.027.5539386
ACGCAGA137100.024.6909267
CGCAGAG139850.024.0771058
TAAGGTG9000.023.8111635
TACATGG138000.023.207472
ACCGTAT1802.1804959E-523.1353388
GTACATG139150.023.109911
GAGTACT46350.023.10455512-13
AGTACTT49700.023.10339412-13
GCAGAGT146050.022.2409659
GGTGTGC11750.021.7711268
CATGGGG62900.021.7128814
ACATGGG141800.021.7023163
Produced by FastQC (version 0.11.5)