##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921138_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1768102
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.385235127837646	31.0	30.0	34.0	26.0	34.0
2	30.4936881469508	31.0	30.0	34.0	27.0	34.0
3	30.575382528836005	31.0	30.0	34.0	27.0	34.0
4	34.06490406096481	37.0	35.0	37.0	30.0	37.0
5	33.6901819012704	37.0	35.0	37.0	28.0	37.0
6	33.546104240592456	37.0	35.0	37.0	28.0	37.0
7	33.35379067497237	37.0	35.0	37.0	27.0	37.0
8	33.34286766261223	37.0	35.0	37.0	27.0	37.0
9	34.71181017837206	39.0	35.0	39.0	27.0	39.0
10-11	34.654935914330736	39.0	35.0	39.0	27.0	39.0
12-13	34.52096937846346	38.0	35.0	39.0	27.0	39.0
14-15	35.56802633558471	39.0	35.0	41.0	27.0	41.0
16-17	35.518820181188644	39.0	35.0	41.0	27.0	41.0
18-19	35.569982953472135	39.0	35.0	41.0	27.0	41.0
20-21	35.575874581896294	39.0	35.0	41.0	26.5	41.0
22-23	35.5305426383772	39.0	35.0	41.0	26.0	41.0
24-25	35.42662018367719	39.0	35.0	41.0	26.0	41.0
26-27	35.30260018935559	39.0	35.0	41.0	25.0	41.0
28-29	35.192126924804114	39.0	34.5	41.0	25.0	41.0
30-31	35.08818693717896	39.0	34.0	41.0	25.0	41.0
32-33	34.9260944787122	39.0	34.0	41.0	24.0	41.0
34-35	34.78600782081577	39.0	34.0	41.0	24.0	41.0
36-37	34.633071225528845	39.0	34.0	41.0	23.5	41.0
38-39	34.44837995771738	39.0	34.0	41.0	22.0	41.0
40-41	34.28355094898372	39.0	33.0	41.0	21.0	41.0
42-43	34.12556289173362	38.0	33.0	41.0	19.5	41.0
44-45	33.916931828593604	38.0	33.0	41.0	17.5	41.0
46-47	33.65163491699008	38.0	33.0	40.5	15.0	41.0
48-49	33.40827141194343	38.0	33.0	40.0	11.5	41.0
50-51	33.173958289736674	38.0	32.5	40.0	9.0	41.0
52-53	32.98653810696442	38.0	32.0	40.0	8.0	41.0
54-55	32.75891577522111	37.5	32.0	40.0	7.0	41.0
56-57	32.47399329902913	37.0	31.5	40.0	4.0	41.0
58-59	32.23970166879512	37.0	31.0	40.0	2.0	41.0
60-61	31.95618324055965	36.0	31.0	40.0	2.0	41.0
62-63	31.653027370592874	36.0	31.0	39.0	2.0	41.0
64-65	31.352959840552188	35.0	30.5	39.0	2.0	41.0
66-67	31.01933400901079	35.0	30.0	39.0	2.0	40.5
68-69	30.65110497018837	35.0	30.0	38.0	2.0	40.0
70-71	30.293979363181535	35.0	29.0	37.0	2.0	40.0
72-73	30.110992182577704	35.0	29.5	37.0	2.0	39.0
74-75	29.917231019477384	35.0	29.0	36.5	2.0	39.0
76-77	29.698840055607654	35.0	29.0	36.0	2.0	39.0
78-79	29.385911559400984	34.5	29.0	36.0	2.0	37.5
80-81	29.102756515178424	34.0	29.0	35.0	2.0	37.0
82-83	28.890473796195018	34.0	29.0	35.0	2.0	37.0
84-85	28.734339704383572	34.0	29.0	35.0	2.0	36.0
86-87	28.5409874543437	34.0	29.0	35.0	2.0	36.0
88-89	28.39290804489786	34.0	29.0	35.0	2.0	35.5
90-91	28.241873206410038	34.0	29.0	35.0	2.0	35.0
92-93	28.113255626655025	34.0	28.5	35.0	2.0	35.0
94-95	28.014470319020056	34.0	28.0	35.0	2.0	35.0
96-97	27.954578977909648	34.0	28.0	35.0	2.0	35.0
98-99	27.90530042950011	34.0	27.5	35.0	2.0	35.0
100-101	27.74856371408437	34.0	27.0	35.0	2.0	35.0
102-103	26.375980005678407	31.5	24.5	34.0	2.0	35.0
104-105	27.45482217654864	33.5	27.0	35.0	2.0	35.0
106-107	27.745557665790773	34.0	27.5	35.0	2.0	35.0
108-109	27.96200275775945	34.0	29.0	35.0	2.0	35.0
110-111	27.94421306010626	34.0	29.0	35.0	2.0	35.0
112-113	27.86024307421178	34.0	29.0	35.0	2.0	35.0
114-115	27.733439303841067	34.0	29.0	35.0	2.0	35.0
116-117	27.633559602330635	34.0	29.0	35.0	2.0	35.0
118-119	27.48244501731235	34.0	27.0	35.0	2.0	35.0
120-121	27.30435461302572	34.0	27.0	35.0	2.0	35.0
122-123	27.100519936067037	34.0	27.0	35.0	2.0	35.0
124-125	26.29079119869781	33.5	25.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	51631.0
3	16777.0
4	8381.0
5	6845.0
6	7832.0
7	9109.0
8	10520.0
9	11126.0
10	11934.0
11	12700.0
12	13391.0
13	14820.0
14	16988.0
15	13730.0
16	9683.0
17	8613.0
18	8300.0
19	8082.0
20	8088.0
21	7923.0
22	8745.0
23	10639.0
24	14583.0
25	19544.0
26	23866.0
27	27887.0
28	33053.0
29	39389.0
30	46237.0
31	55952.0
32	68937.0
33	87179.0
34	113819.0
35	162949.0
36	277383.0
37	398939.0
38	122365.0
39	163.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.342550379633636	19.345949112395893	14.050038628394843	24.261461879575624
2	15.999291894053325	20.622793885177117	38.47260642374259	24.905307797026975
3	19.233953889277256	25.38725360445045	29.413118774362417	25.965673731909877
4	13.299483584515015	17.0333250607131	35.75262145736371	33.91456989740818
5	13.77854920760863	37.896754542887265	33.48021186275215	14.844484386751958
6	31.944163200293225	36.06908160806544	17.284259968392558	14.702495223248778
7	27.975695196678963	32.63053338997365	20.55505998424102	18.838711429106368
8	26.62892386637261	34.15766791803526	19.699976978739556	19.51343123685257
9	26.723283080750516	15.854424955721877	19.524404020131204	37.897887943396405
10-11	25.532009975107485	26.249511752033104	26.639091911106483	21.579386361752924
12-13	26.275648377694594	23.573017531900934	27.04093133892699	23.11040275147749
14-15	23.807964038302647	24.583226907233495	25.186991718140128	26.421817336323727
16-17	23.096362343933464	27.767602540229593	25.755790715902684	23.380244399934263
18-19	23.187239118315013	26.205228481229813	27.913905402183143	22.69362699827203
20-21	24.738585875957593	25.19829343609904	27.440032517832336	22.623088170111032
22-23	23.94771538381682	25.209826122450597	27.297578715027683	23.5448797787049
24-25	23.499809350535912	25.647472734863737	27.498045418699036	23.35467249590132
26-27	23.19494722693183	25.76390409257873	27.473958554317196	23.567190126172243
28-29	23.27622856803425	25.754565010472902	27.60424460055186	23.36496182094099
30-31	23.630301665712484	25.53517321282696	27.414474115945897	23.42005100551466
32-33	23.270618324149584	25.511885322526854	27.30751813579807	23.909978217525488
34-35	23.469272880221624	25.715531096531997	27.31229135705529	23.50290466619109
36-37	23.823429993027375	25.302559504194182	27.309882879735692	23.56412762304275
38-39	23.46498349681874	25.704624172153583	27.04609462427399	23.784297706753687
40-41	23.66108832769201	25.571237110427575	27.20923891059741	23.558435651283002
42-43	23.795924694306812	25.432021099533724	27.014259836096176	23.75779437006328
44-45	23.791392217728454	25.444422003888818	26.92391463612639	23.840271142256338
46-47	23.865894170866326	25.632353174127235	26.547179119305003	23.954573535701435
48-49	23.61005904339183	25.753316629735085	26.672683959737125	23.963940367135965
50-51	23.506841952661468	25.954896150827047	26.54338186935583	23.994880027155656
52-53	23.950400491955794	25.71020601780285	26.313723083085144	24.025670407156213
54-55	23.882597374677704	25.77036279572356	26.258144906136454	24.08889492346228
56-57	23.688125939457233	25.997387481522004	26.058146356475824	24.256340222544935
58-59	23.671101725382	25.740943505258684	26.40686701980064	24.181087749558678
60-61	23.60554585347999	25.952486418508506	26.521769138121915	23.920198589889587
62-63	23.341340482816406	25.87340821130728	26.539174592254867	24.246076713621452
64-65	23.293571452010216	25.961770207103502	26.43800190322949	24.306656437656788
66-67	23.52740998013907	26.173346523035825	26.05708327475382	24.24216022207129
68-69	23.8789382273239	25.595606496908253	26.333552084453014	24.191903191314825
70-71	23.554872087339092	25.51064920428005	26.396894745894667	24.537583962486195
72-73	23.776448515216735	25.555930872311585	26.299114629658547	24.368505982813133
74-75	23.515593355343402	25.88378752667344	26.14743878403056	24.453180333952602
76-77	23.3329948718848	25.68642385876032	26.47030623030218	24.5102750390527
78-79	23.725137025318784	25.792071114570646	26.25237662347213	24.230415236638443
80-81	23.48947727650085	25.807494535900133	26.09436861820431	24.608659569394707
82-83	23.50552724816527	25.751472113150616	26.22553525858787	24.517465380096247
84-85	23.642803525478033	25.740531966826886	26.009055235920915	24.607609271774173
86-87	23.22641572938053	25.609314946526112	26.16098138645471	25.00328793763864
88-89	23.440513514545476	25.641532567700004	26.014025769062698	24.90392814869182
90-91	23.219250051264645	25.729676219568248	26.26910757090431	24.7819661582628
92-93	23.268184235238216	25.679341984681002	26.101095183680854	24.951378596399923
94-95	22.992246334304216	25.741072706384006	26.30979930524169	24.956881654070095
96-97	23.302960663550675	25.45627594204538	26.344489149419108	24.89627424498483
98-99	23.178713917783824	25.496364696745054	26.021649471123197	25.303271914347924
100-101	22.950960626856823	25.591355150231585	26.24999717163215	25.207687051279443
102-103	23.108519510727167	25.335068399545662	26.326669487421995	25.229742602305176
104-105	22.840423994368162	25.482879489355874	26.163525250076862	25.5131712661991
106-107	22.700745823304644	25.583804009966936	26.20441837378277	25.511031792945648
108-109	22.60262078390468	25.684044283849243	26.160013077899478	25.55332185434659
110-111	22.49763279269373	25.720881087569136	26.094430359985203	25.68705575975193
112-113	22.567477792575342	25.9099112042393	26.047388094631387	25.475222908553967
114-115	22.43357094274042	26.06663338909448	25.889621215442116	25.61017445272299
116-117	22.274385189513247	25.951101366063266	26.155432507515002	25.619080936908485
118-119	22.383519680728316	25.783897729895067	25.996027203250634	25.83655538612598
120-121	22.251630046243086	26.152033202718478	25.835158321493534	25.761178429544902
122-123	22.18060560845774	26.161254825747083	25.781020882182343	25.87711868361284
124-125	21.9710043067141	26.225939372126867	25.819601278800675	25.98345504235836
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	33.0
1	40.0
2	101.0
3	150.5
4	115.0
5	95.5
6	152.5
7	365.5
8	614.0
9	701.5
10	606.0
11	469.5
12	443.0
13	466.0
14	508.0
15	605.5
16	674.5
17	747.0
18	892.5
19	987.5
20	1089.5
21	1303.5
22	1540.5
23	1955.0
24	2374.5
25	2809.5
26	3923.5
27	5557.5
28	6757.5
29	8480.5
30	10531.0
31	13456.0
32	17565.0
33	21313.0
34	25954.0
35	30675.0
36	35740.0
37	40085.5
38	42342.0
39	43622.0
40	46552.0
41	50375.5
42	52311.5
43	55360.0
44	58739.5
45	61117.0
46	63909.0
47	66935.0
48	69292.5
49	71934.0
50	77700.0
51	78338.0
52	72958.5
53	70898.5
54	69822.5
55	66797.5
56	61765.0
57	56004.5
58	53326.0
59	50289.0
60	42853.5
61	34768.5
62	27770.0
63	20737.5
64	15508.5
65	12151.0
66	8874.5
67	6477.5
68	4725.5
69	3422.0
70	2586.0
71	1938.5
72	1480.0
73	1311.5
74	1063.5
75	801.5
76	574.0
77	343.0
78	196.5
79	97.0
80	57.0
81	33.5
82	22.0
83	17.5
84	10.5
85	9.0
86	5.0
87	1.5
88	2.0
89	1.5
90	1.0
91	1.5
92	1.0
93	1.5
94	1.5
95	0.5
96	0.5
97	0.5
98	1.5
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07165876176826902
2	2.8278911510761255E-4
3	5.655782302152251E-4
4	0.18347357788181903
5	0.06272262573086847
6	0.009275482975529693
7	0.012499278887756477
8	0.00967138773668035
9	0.016910789083435233
10-11	0.017052183640989038
12-13	0.018353013570484057
14-15	0.025083394510045234
16-17	0.026384224439540253
18-19	0.023669448934507172
20-21	0.025818646209325027
22-23	0.028703095183422674
24-25	0.026016598589900356
26-27	0.024828884306448383
28-29	0.025139952333066755
30-31	0.02545102035968513
32-33	0.022425176828033678
34-35	0.024263306076233157
36-37	0.02683668702371243
38-39	0.0318986121841387
40-41	0.027769891103567555
42-43	0.027147755050330808
44-45	0.026525618997094057
46-47	0.028165795864718214
48-49	0.023697727846017935
50-51	0.029098999944573333
52-53	0.026214550970475686
54-55	0.02881621082946572
56-57	0.025960040766878835
58-59	0.022990755058248904
60-61	0.024828884306448383
62-63	0.029127278856084095
64-65	0.03280353735248306
66-67	0.03175721762658489
68-69	0.02782644892658908
70-71	0.03653635367190354
72-73	0.039929823053194896
74-75	0.02409363260716859
76-77	0.01688251017192447
78-79	0.02053048975681267
80-81	0.024178469341700874
82-83	0.022905918323716617
84-85	0.016118979561133916
86-87	0.015638238065450977
88-89	0.016090700649623153
90-91	0.01778743534026883
92-93	0.02047393193379115
94-95	0.016571442145306096
96-97	0.019201380915806893
98-99	0.016910789083435233
100-101	0.01685423126041371
102-103	0.014365687047466718
104-105	0.016712836702859904
106-107	0.014535360516531285
108-109	0.013234530587036266
110-111	0.011085333312218412
112-113	0.009529993179126544
114-115	0.008172605426610004
116-117	0.007833258488480868
118-119	0.004411510195678757
120-121	0.0030541224431622155
122-123	0.0063061972668997605
124-125	0.05528527200353826
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1768102.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.7863171799062
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.06437819840656	35.64541929340086
2	13.415994159224903	13.0903389267143
3	5.129209211287799	7.507056823919492
4	2.5992189105193324	5.072252727544255
5	1.5015425944448069	3.6627366635861804
6	0.9532123457445805	2.7902231903578505
7	0.6398415345398679	2.185085843425793
8	0.4632765332794015	1.8081244715660998
9	0.32545262585768886	1.4289871528913896
>10	1.7248120898235735	15.12983153374593
>50	0.11661326353948208	3.927922856211557
>100	0.06119379746431907	5.436299281706506
>500	0.004087016785943822	1.321161434703335
>1k	0.0011677190816982346	0.9945598002264163
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2659	0.15038725141422837	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2420	0.1368699317120845	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	2391	0.13522975484446031	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	2320	0.13121414940993223	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	5.6557823021522516E-5	0.0	0.0	0.0	0.0
7	1.1311564604304503E-4	0.0	0.0	0.0	0.0
8	1.1311564604304503E-4	0.0	0.0	0.0	0.0
9	1.1311564604304503E-4	0.0	0.0	0.0	0.0
10-11	1.1311564604304503E-4	0.0	0.0	0.0	0.0
12-13	1.4139455755380627E-4	0.0	0.0	0.0	0.0
14-15	2.2623129208609007E-4	0.0	0.0	0.0	0.0
16-17	2.545102035968513E-4	0.0	0.0	0.0	0.0
18-19	3.9590476115065757E-4	0.0	0.0	0.0	0.0
20-21	5.090204071937026E-4	0.0	0.0	2.8278911510761258E-5	0.0
22-23	6.786938762582701E-4	0.0	0.0	5.655782302152252E-4	0.0
24-25	0.0010180408143874052	0.0	0.0	0.0012725510179842565	0.0
26-27	0.0010180408143874052	0.0	0.0	0.0031389591776944995	0.0
28-29	0.0014987823100703467	0.0	0.0	0.0060234081517921475	0.0
30-31	0.001753292513667198	0.0	0.0	0.015496843507897168	0.0
32-33	0.0018098503366887205	0.0	0.0	0.03543347612298385	0.0
34-35	0.002120918363307094	0.0	0.0	0.0626943468193577	0.0
36-37	0.0023754285669039454	0.0	0.0	0.10350081612938619	0.0
38-39	0.002545102035968513	0.0	0.0	0.17702598605736547	0.0
40-41	0.0026016598589900354	0.0	0.0	0.2659348838471989	0.0
42-43	0.002743054416543842	0.0	0.0	0.3459359245111424	0.0
44-45	0.002771333328054603	0.0	0.0	0.4368526250182399	0.0
46-47	0.0029975646201406934	0.0	0.0	0.5434075635907882	0.0
48-49	0.0029975646201406934	0.0	0.0	0.6604539783338292	0.0
50-51	0.0029975646201406934	0.0	0.0	0.7840045427243452	0.0
52-53	0.003054122443162216	0.0	0.0	0.9026911343350101	0.0
54-55	0.003167238089205261	0.0	0.0	1.0346405354442223	0.0
56-57	0.003280353735248306	0.0	0.0	1.189354460319597	0.0
58-59	0.003280353735248306	0.0	0.0	1.35849063006546	0.0
60-61	0.003280353735248306	0.0	0.0	1.5485249154177758	0.0
62-63	0.003280353735248306	0.0	0.0	1.739209615734839	0.0
64-65	0.003280353735248306	0.0	0.0	1.9366812548144847	0.0
66-67	0.0033369115582698285	0.0	0.0	2.1709437577696313	0.0
68-69	0.0033369115582698285	0.0	0.0	2.438292587192368	0.0
70-71	0.003393469381291351	0.0	0.0	2.723853035628035	0.0
72-73	0.003478306115823635	0.0	0.0	3.0210926745176465	0.0
74-75	0.003534863938845157	0.0	0.0	3.317710177354021	0.0
76-77	0.0035631428503559185	0.0	0.0	3.647640237950073	0.0
78-79	0.0035631428503559185	0.0	0.0	4.024739522946074	0.0
80-81	0.0039024897884850536	0.0	0.0	4.431616501762908	0.0
82-83	0.004128721080571144	0.0	0.0	4.864001058762447	0.0
84-85	0.004326673461146472	0.0	0.0	5.294462649779255	0.0
86-87	0.00449634693021104	0.0	0.0	5.756964247537756	0.0
88-89	0.004524625841721801	0.0	0.0	6.263863736368151	0.0
90-91	0.004637741487764846	0.0	0.0	6.803651599285562	0.0
92-93	0.004750857133807891	0.0	0.0	7.376016768263369	0.0
94-95	0.004750857133807891	0.0	0.0	7.965038216121015	0.0
96-97	0.004750857133807891	0.0	0.0	8.56107283403333	0.0
98-99	0.005090204071937026	0.0	0.0	9.206029968859262	0.0
100-101	0.005372993187044638	0.0	0.0	9.924286042321087	0.0
102-103	0.00562750339064149	0.0	0.0	10.655324183785776	0.0
104-105	0.006362755089921283	0.0	0.0	11.392159502110172	0.0
106-107	0.006730380939561179	0.0	0.0	12.143869527889228	0.0
108-109	0.006900054408625747	0.0	0.0	12.919390397160345	0.0
110-111	0.0070414489661795535	0.0	0.0	13.737866933016306	5.6557823021522516E-5
112-113	0.007069727877690314	0.0	0.0	14.597347890562876	5.6557823021522516E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	9310	0.0	36.719574	1
GGTATCA	6505	0.0	31.91719	1
TATCAAC	11665	0.0	29.193352	2
GTAAGGT	685	0.0	28.731344	4
TCAACGC	11960	0.0	28.423416	4
CAACGCA	12125	0.0	28.033346	5
ATCAACG	12230	0.0	27.84468	3
AACGCAG	12415	0.0	27.553938	6
ACGCAGA	13710	0.0	24.690926	7
CGCAGAG	13985	0.0	24.077105	8
TAAGGTG	900	0.0	23.811163	5
TACATGG	13800	0.0	23.20747	2
ACCGTAT	180	2.1804959E-5	23.135338	8
GTACATG	13915	0.0	23.10991	1
GAGTACT	4635	0.0	23.104555	12-13
AGTACTT	4970	0.0	23.103394	12-13
GCAGAGT	14605	0.0	22.240965	9
GGTGTGC	1175	0.0	21.771126	8
CATGGGG	6290	0.0	21.712881	4
ACATGGG	14180	0.0	21.702316	3
>>END_MODULE