FastQCFastQC Report
Mon 15 Aug 2016
SRR2921137_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921137_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2680276
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA29910.11159298520003164No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT27730.10345949446997249No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA85900.027.9319861
GTACATG138600.024.6569081
TCAACGC96750.024.5392154
AACGCAG98250.024.1026556
CAACGCA99200.023.8727235
TACATGG142850.023.2868522
ATCAACG101850.023.254193
CATGGGG76000.023.2531364
TATCAAC103800.023.0466542
GGTATCA67900.021.9209921
ACATGGG147600.021.7310963
ACGCAGA108500.021.7167
CGCAGAG113650.020.7839158
GTATAGG10300.019.6531261
GCAGAGT122850.017.968229
TATAGGG6900.017.2488482
TGTACGC3256.489423E-616.4792842
ATGGGGC37650.016.2780675
GTATAGC12100.016.2374741
ATGGGGG34550.016.188645
Produced by FastQC (version 0.11.5)