FastQCFastQC Report
Mon 15 Aug 2016
SRR2921137_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921137_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2680276
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27320.1019298012592733No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT27030.10084782313463239No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA106400.029.7714961
GGTATCA80250.028.470921
TCAACGC127300.024.2662644
CAACGCA129450.023.7275965
ATCAACG131200.023.5899473
AACGCAG132250.023.5249316
TATCAAC133800.023.2643852
TATACCG2204.1651074E-621.6457885
GTACATG143800.021.3231831
TACCGTG5100.020.9954267
ACGCAGA147950.020.7068777
GAGTACT57100.020.2649912-13
TACATGG150750.019.9365672
CGCAGAG154150.019.8365558
CATGGGG78700.019.549994
GTACTTT58750.019.34178214-15
ACATGGG153150.019.313373
GTACCGT4657.2759576E-1219.1893676
GTATAGG9550.018.7398321
GCAGAGT162050.017.9156959
Produced by FastQC (version 0.11.5)