##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921137_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2680276
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.570913592480775	31.0	30.0	34.0	27.0	34.0
2	30.654729960645845	31.0	31.0	34.0	27.0	34.0
3	30.73571639637112	31.0	31.0	34.0	27.0	34.0
4	34.25912517964568	37.0	35.0	37.0	30.0	37.0
5	33.904152781280736	37.0	35.0	37.0	29.0	37.0
6	33.77849034950132	37.0	35.0	37.0	29.0	37.0
7	33.59868423998125	37.0	35.0	37.0	28.0	37.0
8	33.58977135190555	37.0	35.0	37.0	28.0	37.0
9	34.97860257674956	39.0	35.0	39.0	28.0	39.0
10-11	34.94390409793618	39.0	35.0	39.0	27.0	39.0
12-13	34.821103498296445	39.0	35.0	39.0	27.0	39.0
14-15	35.90446674148483	39.0	36.0	41.0	27.0	41.0
16-17	35.86008679703135	39.0	36.0	41.0	27.0	41.0
18-19	35.91074352044342	39.5	36.0	41.0	27.0	41.0
20-21	35.913347170216795	40.0	36.0	41.0	27.0	41.0
22-23	35.87951837795809	40.0	36.0	41.0	27.0	41.0
24-25	35.786920638023844	40.0	35.0	41.0	27.0	41.0
26-27	35.68414596108759	39.0	35.0	41.0	27.0	41.0
28-29	35.58928595413308	39.0	35.0	41.0	27.0	41.0
30-31	35.50663812234262	39.0	35.0	41.0	26.0	41.0
32-33	35.36828837776408	39.0	35.0	41.0	25.0	41.0
34-35	35.252142689782694	39.0	35.0	41.0	25.0	41.0
36-37	35.12178689806572	39.0	35.0	41.0	25.0	41.0
38-39	34.95635412173971	39.0	34.0	41.0	24.0	41.0
40-41	34.81739324606869	39.0	34.0	41.0	24.0	41.0
42-43	34.6821879537779	39.0	34.0	41.0	23.5	41.0
44-45	34.51507456694758	39.0	34.0	41.0	23.0	41.0
46-47	34.29792230352396	38.5	33.0	41.0	21.5	41.0
48-49	34.09352432361443	38.0	33.0	41.0	20.0	41.0
50-51	33.88303499341113	38.0	33.0	40.0	18.5	41.0
52-53	33.70628211423003	38.0	33.0	40.0	18.0	41.0
54-55	33.503180829138486	38.0	33.0	40.0	16.0	41.0
56-57	33.23444078147176	38.0	32.5	40.0	14.0	41.0
58-59	33.01131208129312	37.0	32.0	40.0	13.0	41.0
60-61	32.73992771639936	37.0	32.0	40.0	11.5	41.0
62-63	32.44276839400122	36.0	31.5	40.0	8.0	41.0
64-65	32.15059251360681	36.0	31.0	39.0	8.0	41.0
66-67	31.821107415803446	35.5	31.0	39.0	7.0	41.0
68-69	31.460013259828465	35.0	31.0	38.5	6.5	40.0
70-71	31.09542935130561	35.0	30.5	37.5	2.0	40.0
72-73	30.904070886729578	35.0	30.5	37.0	2.0	39.5
74-75	30.697443285691474	35.0	30.0	37.0	2.0	39.0
76-77	30.462678843522085	35.0	30.0	36.0	2.0	39.0
78-79	30.14141342160285	35.0	30.0	36.0	2.0	38.5
80-81	29.845277501272257	35.0	30.0	35.5	2.0	37.0
82-83	29.624713648892875	34.0	30.0	35.0	2.0	37.0
84-85	29.46528641080247	34.0	30.0	35.0	2.0	36.0
86-87	29.26586515717038	34.0	30.0	35.0	2.0	36.0
88-89	29.122610320728164	34.0	29.0	35.0	2.0	36.0
90-91	28.9652183207998	34.0	29.0	35.0	2.0	35.5
92-93	28.848073668532642	34.0	29.0	35.0	2.0	35.0
94-95	28.74290408898188	34.0	29.0	35.0	2.0	35.0
96-97	28.679431521231397	34.0	29.0	35.0	2.0	35.0
98-99	28.635135336808595	34.0	29.0	35.0	2.0	35.0
100-101	28.490023042403095	34.0	29.0	35.0	2.0	35.0
102-103	27.11283763313927	32.0	26.0	34.0	2.0	35.0
104-105	28.213820703539486	34.0	29.0	35.0	2.0	35.0
106-107	28.492860063665084	34.0	29.0	35.0	2.0	35.0
108-109	28.70071029998403	34.0	29.0	35.0	2.0	35.0
110-111	28.676347697028216	34.0	29.5	35.0	2.0	35.0
112-113	28.605914838621096	34.0	29.5	35.0	2.0	35.0
114-115	28.49209409777202	34.0	29.0	35.0	2.0	35.0
116-117	28.392654711678947	34.0	29.0	35.0	2.0	35.0
118-119	28.250598818927603	34.0	29.0	35.0	2.0	35.0
120-121	28.076750491367306	34.0	29.0	35.0	2.0	35.0
122-123	27.894442587255938	34.0	29.0	35.0	2.0	35.0
124-125	27.099831323341327	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	68581.0
3	22245.0
4	11015.0
5	8935.0
6	9843.0
7	11908.0
8	13136.0
9	14619.0
10	15905.0
11	16719.0
12	17427.0
13	19154.0
14	20570.0
15	17311.0
16	12635.0
17	11508.0
18	11035.0
19	10498.0
20	10742.0
21	10809.0
22	12108.0
23	15226.0
24	20769.0
25	27739.0
26	34565.0
27	41195.0
28	48894.0
29	58276.0
30	69346.0
31	83235.0
32	103215.0
33	130245.0
34	170661.0
35	245215.0
36	424446.0
37	647909.0
38	212433.0
39	204.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.68657109306012	19.52752112881154	13.819054855566115	24.96685292256223
2	15.899168216885936	20.460469222084672	38.408575865231285	25.231786695798103
3	19.127121018618737	24.26879213448561	29.37916028201773	27.224926564877922
4	13.508827468101178	16.777178985105596	35.92704953600524	33.786944010787984
5	14.117421288101472	37.268869144980584	34.0948929718299	14.51881659508805
6	32.78738805970149	36.12914179104478	16.933246268656717	14.150223880597016
7	28.652225407233217	31.788930540308385	20.84902724490413	18.709816807554272
8	26.28551620276913	35.22810295920781	19.631272542196303	18.85510829582675
9	26.36305388553433	15.865560254543947	19.705885535827104	38.065500324094614
10-11	25.29503321724184	25.853324690842506	27.495211365994933	21.35643072592072
12-13	25.950772144940764	23.33598781674213	27.53695037173341	23.1762896665837
14-15	23.461898737484	24.737513854834916	24.94463663946081	26.85595076822027
16-17	23.225266398038773	27.710938021894034	25.800239070617636	23.26355650944956
18-19	23.181920130586704	26.752398399909836	27.510627522998774	22.55505394650468
20-21	24.147607149721313	25.805034063619008	27.26847281907936	22.77888596758032
22-23	23.752006677386362	25.71275184116091	26.976797649852593	23.558443831600137
24-25	23.376294767562282	26.025093906218803	27.152938849464554	23.44567247675436
26-27	23.220910255785874	26.086076268708492	27.20028676290988	23.492726712595754
28-29	23.306254149485603	25.916954681552756	27.483822837350612	23.29296833161103
30-31	23.629535459184485	26.027952201470516	27.008512846521892	23.33399949282311
32-33	23.37596303066864	26.038572913053365	27.055878869685756	23.529585186592236
34-35	23.43085303898661	26.117281898042254	27.120449954302217	23.33141510866892
36-37	23.688291244352452	25.937662578065463	26.89762424229758	23.476421935284506
38-39	23.54042624342147	26.089373548394562	26.957621612018684	23.41257859616528
40-41	23.565456033878384	26.0147495779477	27.11823417086946	23.301560217304452
42-43	23.710543011755924	25.807072214965476	26.82608695652174	23.656297816756858
44-45	23.678831958789242	25.91164116963106	26.729537978152223	23.679988893427474
46-47	23.76052292889595	26.00803934693229	26.59230180561545	23.639135918556313
48-49	23.714776091107087	26.084016402619525	26.526325846067955	23.674881660205436
50-51	23.57028746988397	26.242946956785744	26.417816336580728	23.768949236749553
52-53	23.630141012275153	26.198699505153634	26.385580095826917	23.7855793867443
54-55	23.66823205110645	26.160518648203517	26.323445273933572	23.847804026756467
56-57	23.6819703296609	26.160687528433357	26.22711755988313	23.930224582022618
58-59	23.529377738410208	25.96837163616727	26.58837355064033	23.913877074782192
60-61	23.699254781725518	26.260934436578076	26.390321809572164	23.649488972124242
62-63	23.468878968083654	26.244792759089393	26.45808343677527	23.828244836051685
64-65	23.430996542437256	26.12878376199547	26.510686944266954	23.929532751300318
66-67	23.531777341023723	26.23656303254282	26.257127629637207	23.97453199679625
68-69	23.60360733566475	25.936705362542682	26.377237860491448	24.082449441301115
70-71	23.482011440165316	25.83592128303333	26.532796838810345	24.149270437991007
72-73	23.57222053325465	25.91363347686758	26.464386205856595	24.04975978402117
74-75	23.45620821367727	26.081438895637522	26.330118247246276	24.13223464343893
76-77	23.377862826869354	25.888405284130904	26.550274380965906	24.183457508033836
78-79	23.422519043517593	25.99871177302127	26.41813190668042	24.160637276780715
80-81	23.382819350132866	26.132318944938543	26.220952287831835	24.26390941709676
82-83	23.38015438501164	26.00851358570562	26.364574258205376	24.246757771077355
84-85	23.382349483934792	25.8864570711886	26.276132110006838	24.455061334869768
86-87	23.23661230295089	25.89792861126333	26.414586350093224	24.45087273569256
88-89	23.246677959833814	25.849734951394538	26.39216521136066	24.511421877410985
90-91	23.194699365184384	25.927654940032753	26.450687926823147	24.426957767959713
92-93	23.216687076636187	25.88115370843517	26.47294641902529	24.42921279590335
94-95	23.058590719507066	25.78589092515728	26.53740419502551	24.618114160310142
96-97	23.186585633445834	25.653052329269734	26.49269103833081	24.66767099895362
98-99	23.126094415716114	25.681175536584387	26.29967376266304	24.893056285036465
100-101	22.92145678403764	25.853433090297166	26.462009280987488	24.763100844677705
102-103	22.958802141016953	25.670933575337106	26.400620482952764	24.969643800693174
104-105	22.836532361708382	25.844083004076467	26.27189925113032	25.047485383084826
106-107	22.75640026964412	25.791295972857963	26.3701949583714	25.082108799126512
108-109	22.732891210309443	25.795686175368417	26.313490660469856	25.15793195385228
110-111	22.729635395366472	25.95085942094312	26.21931652303183	25.100188660658578
112-113	22.539766406237028	26.05421391723005	26.26874793959465	25.137271736938267
114-115	22.458042858904566	26.142017625571984	26.199964813780756	25.19997470174269
116-117	22.424554607356352	25.98586743311974	26.238250690005525	25.351327269518382
118-119	22.483060022025096	25.856989106289653	26.179884553870536	25.480066317814714
120-121	22.332958794441204	26.007799089950712	26.210620947923545	25.448621167684536
122-123	22.225045521029223	26.118306169965077	26.09830676696218	25.55834154204352
124-125	22.08315749334079	26.26197430686068	26.056621815258534	25.59824638453999
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	61.0
1	73.0
2	117.0
3	182.5
4	163.5
5	111.5
6	155.5
7	416.5
8	672.0
9	687.5
10	562.0
11	400.0
12	382.5
13	428.0
14	480.5
15	569.0
16	609.0
17	710.5
18	872.5
19	1008.0
20	1164.0
21	1309.5
22	1516.0
23	1894.5
24	2415.5
25	3151.5
26	4500.0
27	6494.0
28	8620.5
29	11676.0
30	15664.0
31	20837.5
32	28637.5
33	36705.0
34	43966.5
35	50845.0
36	58922.0
37	66651.5
38	69388.0
39	71567.5
40	75170.0
41	77644.5
42	80047.5
43	85693.5
44	92732.0
45	97513.5
46	100698.5
47	104153.5
48	107826.5
49	111199.5
50	116000.5
51	115148.0
52	108740.5
53	105813.5
54	104160.5
55	99144.5
56	91805.0
57	84248.5
58	77846.0
59	70886.0
60	60756.5
61	50112.5
62	40070.0
63	30101.5
64	21520.0
65	15230.5
66	10847.5
67	7685.5
68	5625.0
69	4205.0
70	3311.0
71	2597.5
72	1959.5
73	1606.0
74	1252.0
75	865.0
76	594.0
77	358.0
78	193.5
79	117.5
80	76.5
81	54.0
82	35.0
83	15.5
84	10.5
85	9.0
86	6.0
87	3.5
88	2.5
89	2.5
90	2.0
91	2.5
92	4.0
93	2.5
94	2.0
95	3.0
96	3.5
97	3.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07689506603051327
2	3.7309590504858457E-4
3	7.088822195923107E-4
4	0.17561624250636876
5	0.06973162465358045
6	0.010297446979340935
7	0.013506071762758762
8	0.011267496332467253
9	0.018766724023943804
10-11	0.01839362811889522
12-13	0.019886011739089555
14-15	0.02634057089643007
16-17	0.027347929840061246
18-19	0.025258592771789178
20-21	0.02727331065905153
22-23	0.02926937375106146
24-25	0.027646406564100112
26-27	0.026508464053701934
28-29	0.0271240722970321
30-31	0.027515822997333107
32-33	0.024232579032905568
34-35	0.026676357210973795
36-37	0.029623814860857613
38-39	0.034138275311945486
40-41	0.030202113513682922
42-43	0.02895224223177016
44-45	0.029157444979546886
46-47	0.03094830532378009
48-49	0.02626595171542035
50-51	0.03161987795286754
52-53	0.02826201480743028
54-55	0.03167584233862483
56-57	0.028541836736216717
58-59	0.02565034347209019
60-61	0.027068107911274807
62-63	0.03111619848105195
64-65	0.034772538350528084
66-67	0.034100965721440626
68-69	0.02953054088459547
70-71	0.038484842605761495
72-73	0.04195463452271333
74-75	0.026900214754002946
76-77	0.019270403495759394
78-79	0.023281184475031677
80-81	0.02602343937713877
82-83	0.02523993797653674
84-85	0.0178712938518272
86-87	0.016994518474963027
88-89	0.018076496599603923
90-91	0.019456951448283685
92-93	0.023187910498769528
94-95	0.018020532213846634
96-97	0.02059489395868187
98-99	0.017852639056574773
100-101	0.018524211685662224
102-103	0.015968504736079418
104-105	0.018804033614448663
106-107	0.016696041750924158
108-109	0.015054419768710386
110-111	0.012386784047613007
112-113	0.010502649727117655
114-115	0.009308742830962185
116-117	0.008394657863593153
118-119	0.0056524029614860565
120-121	0.0034138275311945488
122-123	0.007312679738952257
124-125	0.05475182406587978
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2680276.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.03027016408602
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.94436314053125	30.59553028344891
2	14.475614258505523	13.036816417031991
3	6.411121386233748	8.660835842305662
4	3.376484108216829	6.081759663909875
5	1.947192502147734	4.384130221659756
6	1.3412831841602837	3.6239006489569867
7	0.9251903865036107	2.916310114023093
8	0.6326729091716683	2.2791545620398783
9	0.46000943831551466	1.8642914356839382
>10	2.310491116090483	18.106013757641065
>50	0.1251251911437149	3.8624305406332597
>100	0.04870340696899454	3.668498427026321
>500	0.0010826969589334897	0.333890829116064
>1k	6.662750516409047E-4	0.5864372565231437
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2732	0.1019298012592733	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	2703	0.10084782313463239	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	3.7309590504858456E-5
7	3.7309590504858456E-5	0.0	0.0	0.0	3.7309590504858456E-5
8	3.7309590504858456E-5	0.0	0.0	0.0	3.7309590504858456E-5
9	3.7309590504858456E-5	0.0	0.0	0.0	7.461918100971691E-5
10-11	3.7309590504858456E-5	0.0	0.0	3.7309590504858456E-5	7.461918100971691E-5
12-13	3.7309590504858456E-5	0.0	0.0	9.327397626214613E-5	9.327397626214613E-5
14-15	3.7309590504858456E-5	0.0	0.0	1.305835667670046E-4	1.8654795252429226E-4
16-17	7.461918100971691E-5	0.0	0.0	1.8654795252429229E-4	2.2385754302915072E-4
18-19	1.8654795252429226E-4	0.0	0.0	1.8654795252429229E-4	2.611671335340092E-4
20-21	2.611671335340092E-4	0.0	0.0	2.4251233828157995E-4	2.798219287864384E-4
22-23	2.9847672403886765E-4	0.0	0.0	5.036794718155891E-4	4.10405495553443E-4
24-25	4.10405495553443E-4	0.0	0.0	8.394657863593152E-4	5.223342670680184E-4
26-27	4.10405495553443E-4	0.0	0.0	0.001492383620194338	5.782986528253061E-4
28-29	5.409890623204476E-4	0.0	0.0	0.0035630658932139826	5.969534480777353E-4
30-31	5.596438575728768E-4	0.0	0.0	0.010763816860651663	5.969534480777353E-4
32-33	5.596438575728768E-4	0.0	0.0	0.02634057089643007	6.715726290874522E-4
34-35	5.782986528253061E-4	0.0	0.0	0.048129371751267414	6.902274243398814E-4
36-37	6.342630385825938E-4	0.0	0.0	0.08528972389410644	7.275370148447399E-4
38-39	7.088822195923107E-4	0.0	0.0	0.1464401427315694	7.461918100971691E-4
40-41	7.088822195923107E-4	0.0	0.0	0.21973483327836385	7.461918100971691E-4
42-43	7.461918100971691E-4	0.0	0.0	0.28655630987256536	7.461918100971691E-4
44-45	7.835014006020276E-4	0.0	0.0	0.36236939777843774	7.461918100971691E-4
46-47	9.327397626214613E-4	0.0	0.0	0.45267726159544763	8.954301721166029E-4
48-49	9.327397626214613E-4	0.0	0.0	0.5481525036973804	8.954301721166029E-4
50-51	9.327397626214613E-4	0.0	0.0	0.652917833835023	8.954301721166029E-4
52-53	9.327397626214613E-4	0.0	0.0	0.7597351914504327	9.88704148378749E-4
54-55	9.327397626214613E-4	0.0	0.0	0.8784169988463875	0.0010819781246408952
56-57	9.327397626214613E-4	0.0	0.0	1.0193353221832377	0.0010819781246408952
58-59	9.327397626214613E-4	0.0	0.0	1.1726963939534585	0.0011006329198933245
60-61	9.327397626214613E-4	0.0	0.0	1.3327918468098061	0.0011192877151457537
62-63	9.327397626214613E-4	0.0	0.0	1.4960213052685618	0.0011565973056506121
64-65	0.0010073589436311783	0.0	0.0	1.6809276358106402	0.0011565973056506121
66-67	0.0010073589436311783	0.0	0.0	1.8940213619791395	0.0011565973056506121
68-69	0.0010446685341360367	0.0	0.0	2.1264787656196598	0.0011565973056506121
70-71	0.0010446685341360367	0.0	0.0	2.388410745759019	0.0011565973056506121
72-73	0.0010633233293884659	0.0	0.0	2.6461640517618337	0.001380454848679763
74-75	0.0010819781246408952	0.0	0.0	2.9161549034502414	0.001380454848679763
76-77	0.0010819781246408952	0.0	0.0	3.2183998961300997	0.001380454848679763
78-79	0.0010819781246408952	0.0	0.0	3.5586260519439046	0.0014177644391846214
80-81	0.0013244904629224753	0.0	0.0	3.927916378760993	0.0014550740296894798
82-83	0.001380454848679763	0.0	0.0	4.315693607673239	0.001641621982213772
84-85	0.0014177644391846214	0.0	0.0	4.707220450431224	0.001641621982213772
86-87	0.0014737288249419092	0.0	0.0	5.13585168094629	0.001641621982213772
88-89	0.0014923836201943383	0.0	0.0	5.606139069260031	0.0016789315727186304
90-91	0.0015670028012040552	0.0	0.0	6.108251538274416	0.0016789315727186304
92-93	0.0015670028012040552	0.0	0.0	6.620698763858647	0.0017348959584759182
94-95	0.0015670028012040552	0.0	0.0	7.146857263953414	0.0018654795252429227
96-97	0.0015670028012040552	0.0	0.0	7.704355820072261	0.0018654795252429227
98-99	0.0017348959584759182	0.0	0.0	8.310375498642678	0.0018654795252429227
100-101	0.0017722055489807764	0.0	0.0	8.961259959795186	0.0018654795252429227
102-103	0.0017908603442332058	0.0	0.0	9.625669147505704	0.0019027891157477812
104-105	0.0019400987062526396	0.0	0.0	10.300525020557584	0.0019400987062526396
106-107	0.0020147178872623565	0.0	0.0	10.979783425289039	0.002033372682514786
108-109	0.0020520274777672152	0.0	0.0	11.697638601397767	0.0021266466587769317
110-111	0.002107991863524503	0.0	0.0	12.473211714017511	0.0022385754302915073
112-113	0.0021639562492817904	0.0	0.0	13.27107730696391	0.0023131946113012242
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	10640	0.0	29.771496	1
GGTATCA	8025	0.0	28.47092	1
TCAACGC	12730	0.0	24.266264	4
CAACGCA	12945	0.0	23.727596	5
ATCAACG	13120	0.0	23.589947	3
AACGCAG	13225	0.0	23.524931	6
TATCAAC	13380	0.0	23.264385	2
TATACCG	220	4.1651074E-6	21.645788	5
GTACATG	14380	0.0	21.323183	1
TACCGTG	510	0.0	20.995426	7
ACGCAGA	14795	0.0	20.706877	7
GAGTACT	5710	0.0	20.26499	12-13
TACATGG	15075	0.0	19.936567	2
CGCAGAG	15415	0.0	19.836555	8
CATGGGG	7870	0.0	19.54999	4
GTACTTT	5875	0.0	19.341782	14-15
ACATGGG	15315	0.0	19.31337	3
GTACCGT	465	7.2759576E-12	19.189367	6
GTATAGG	955	0.0	18.739832	1
GCAGAGT	16205	0.0	17.915695	9
>>END_MODULE