FastQCFastQC Report
Mon 15 Aug 2016
SRR2921136_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921136_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1826516
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT25480.1395005573452409No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA23650.12948148277923652No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21650.11853167451037931No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA67450.024.6220651
TACCGTA2201.5399019E-724.3406287
GTACATG93100.023.2091481
TACATGG90800.023.1994112
CATGGGG46050.023.129124
TACGTTA1852.6907077E-522.5144885
TATACCG1350.002555211522.037995
ACATGGG94400.021.8734363
TATCAAC73900.021.821492
TCAACGC73100.021.57074
ATCAACG74150.021.5071663
AACGCAG74550.021.3894256
CAACGCA75100.020.9962485
GTATAAT12150.020.0867581
GTTACGT2107.1494884E-519.8331058
GTGTAGC14600.019.1622831
ACGCAGA82050.019.1442057
GTATAGA7900.018.8371491
CGTTACG1600.006834619718.5935367
GGTATCA55050.018.4901751
Produced by FastQC (version 0.11.5)