FastQCFastQC Report
Mon 15 Aug 2016
SRR2921136_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921136_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1826516
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33750.18477801453696546No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29630.1622214095031196No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA24700.1352301321203866No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT24470.133970904169468No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21900.11990040054398647No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA91250.030.393021
GGTATCA67900.028.108261
TATCAAC107200.025.304142
TCAACGC107900.025.1959254
ATCAACG108900.025.0193043
CAACGCA108950.024.856775
GAGTACT53250.024.74506612-13
AACGCAG109850.024.6944486
AGTACTT56500.023.74283612-13
GTACCGT2853.2105163E-922.9605546
GTACTTT57800.022.69484114-15
ACGCAGA120500.022.3144347
TACCGTA2204.1865387E-621.6322577
CGCAGAG124100.021.6185918
CATGGGG47800.021.3282434
GTACATG101450.020.8703671
GCAGAGT129650.020.4184139
TACATGG103350.019.9590842
TACTTTT69000.019.44213314-15
CCGTATG2158.578137E-519.3684169
Produced by FastQC (version 0.11.5)