FastQCFastQC Report
Mon 15 Aug 2016
SRR2921135_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921135_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3387617
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA85200.027.6055891
GTACATG145450.026.404961
CATGGGG83250.025.8750784
TACATGG143550.025.8669432
ACATGGG141400.025.7552473
TCAACGC91050.025.3576554
ATCAACG92450.024.9740263
CAACGCA93400.024.6544765
AACGCAG95050.024.4135726
TATCAAC99100.023.5383572
GGTATCA64700.022.8237461
ACGCAGA106950.021.6415147
CGCAGAG107450.021.540818
GTATAAT16750.018.1298791
GCAGAGT125350.017.3730329
ATGGGGA50450.016.9837275
GTATAGC16300.016.8038861
AGAGTAC106700.016.34104310-11
ATTATAC14600.015.8955483
GTATTAT15150.015.7212441
Produced by FastQC (version 0.11.5)