FastQCFastQC Report
Mon 15 Aug 2016
SRR2921135_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921135_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3387617
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA104700.032.2957841
GGTATCA74150.028.4709221
TCAACGC121850.027.2519594
ATCAACG122100.027.2441793
CAACGCA124450.026.7862995
TATCAAC127600.026.1158082
AACGCAG128600.025.9061976
ACGCAGA144000.022.9704177
CGCAGAG145800.022.6868348
GTACATG148250.022.6476481
CATGGGG88350.021.457974
TACATGG150400.021.205492
ACATGGG145950.021.0766123
GCAGAGT163050.019.5569259
GAGTACT65000.019.1743612-13
AGAGTAC147500.018.39188610-11
TATACTG18600.018.242385
GTACTTT69600.018.03570214-15
AGTACTT74300.017.97536512-13
CAGAGTA158250.016.25907310-11
Produced by FastQC (version 0.11.5)