FastQCFastQC Report
Mon 15 Aug 2016
SRR2921134_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921134_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5104967
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66050.045.247181
GTATCAA103750.043.667391
TCAACGC122950.036.0554854
CAACGCA125150.035.517115
ATCAACG128450.034.5133443
AACGCAG132950.033.5656626
TATCAAC132900.033.5368042
GTACATG223950.028.8163431
TACATGG223150.028.3732832
ACGCAGA157450.028.0025677
ACATGGG230150.027.1224783
CATGGGG160650.026.4460664
CGCAGAG167200.026.1919698
GCAGAGT188650.022.0468719
AGAGTAC163750.019.96974410-11
CAGAGTA177750.019.70257610-11
ATGGGGG80450.018.7869175
ATGGGGA79200.018.4072465
GGACCGT9500.016.2844126
GTATTAG16000.015.9995991
Produced by FastQC (version 0.11.5)