FastQCFastQC Report
Mon 15 Aug 2016
SRR2921134_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921134_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5104967
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79200.053.480731
GTATCAA126400.046.1117551
TCAACGC156500.036.965614
CAACGCA161200.035.8568535
ATCAACG163600.035.3231663
TATCAAC164700.035.19382
AACGCAG170500.034.0193376
ACGCAGA200300.028.6613257
GTACATG221600.028.051891
TACATGG220000.027.7297862
CGCAGAG211100.027.1101848
ACATGGG224650.026.4671923
CATGGGG155700.025.4343074
GCAGAGT230900.023.8328639
AGAGTAC204450.021.78026210-11
CAGAGTA220050.021.29058610-11
GAGTACT91800.020.96466412-13
GTACTTT99550.019.1237714-15
GTGGTAT49350.018.132941
TATACCG5102.910383E-1117.5081045
Produced by FastQC (version 0.11.5)