FastQCFastQC Report
Mon 15 Aug 2016
SRR2921133_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921133_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1876136
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA30950.1649667188306178No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT30700.1636341928303705No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCGC1107.749136E-427.0478067
CATGGGG53550.025.6730064
GTATCAA89200.023.0951461
GTACATG116650.022.5604111
TACATGG113350.022.3147282
GAGTACT20700.021.84876412-13
TATCAAC100050.020.5223082
AACGCAG98800.020.4172616
ACATGGG121350.020.3041483
TCAACGC100900.019.9365354
ATCAACG102550.019.6737983
CAACGCA102800.019.6259545
TATACTG7600.019.57725
ACGCAGA109450.018.3218297
CCTATAC5850.018.3122123
CGCAGAG109750.018.217538
GTGTAGC11200.018.074671
ATGGGGC27800.017.9828575
GGTATCA74900.017.9653681
TATTCCG2000.001172765517.8544065
Produced by FastQC (version 0.11.5)