FastQCFastQC Report
Mon 15 Aug 2016
SRR2921133_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921133_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1876136
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34560.18420839427418909No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32850.17509391643249744No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT30700.1636341928303705No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA29490.1571847669891735No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT25440.13559784578516695No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21030.11209208714080428No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA109100.028.438731
GGTATCA85050.025.6766511
CATGGGG54400.024.1169974
TATCAAC127900.024.1024632
GAGTACT57000.023.6407412-13
TCAACGC130650.023.5982914
ATCAACG131400.023.4617183
AGTACTT60050.023.38119312-13
CAACGCA131300.023.3034885
AACGCAG133150.023.1865466
GTACTTT62000.021.7827914-15
GTACATG124700.021.6273651
TACATGG122150.021.3807052
ACGCAGA146000.020.7791217
CGCAGAG147250.020.4433168
TACTTTT71750.019.81779514-15
ACATGGG126750.019.7123
TCCGTAC3051.6489685E-719.5054658
GCAGAGT151050.019.3781419
GTATAGG5101.8189894E-1218.7189481
Produced by FastQC (version 0.11.5)