FastQCFastQC Report
Mon 15 Aug 2016
SRR2921132_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921132_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3782068
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA80800.036.110211
GGTATCA57500.031.2740671
TCAACGC92800.030.6509364
CAACGCA97000.029.3233955
ATCAACG99350.028.6915763
AACGCAG99100.028.5203656
TATCAAC101350.028.3602582
GTACATG155650.026.5112041
TACATGG156100.025.8472042
ACATGGG156550.025.316753
CATGGGG104850.024.9149444
ACGCAGA116800.023.8926937
CGCAGAG119550.023.043858
GCAGAGT136000.019.3377979
AGAGTAC115700.018.46359610-11
CCGTATG5303.6379788E-1217.962549
GTATAGG11500.016.5690441
CAGAGTA128400.016.56785410-11
ACCGTAT5105.5479177E-1016.3335848
TACCGTA3307.5114367E-616.2279517
Produced by FastQC (version 0.11.5)