##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921132_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3782068
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.36751666019754	31.0	30.0	34.0	25.0	34.0
2	29.470270761921785	31.0	30.0	34.0	25.0	34.0
3	29.54450184396473	31.0	30.0	34.0	25.0	34.0
4	32.98902981120382	35.0	33.0	37.0	26.0	37.0
5	33.044412474868246	35.0	35.0	37.0	26.0	37.0
6	33.12037171198403	37.0	35.0	37.0	26.0	37.0
7	33.08037190235606	37.0	35.0	37.0	26.0	37.0
8	33.049314819299916	37.0	35.0	37.0	26.0	37.0
9	34.45680352653628	39.0	35.0	39.0	27.0	39.0
10-11	34.38025175644647	38.5	35.0	39.0	26.5	39.0
12-13	34.357550683911555	38.5	35.0	39.0	26.0	39.0
14-15	35.39051175177178	39.0	35.0	41.0	26.0	41.0
16-17	35.28281604138265	39.0	35.0	41.0	25.0	41.0
18-19	35.33955127194963	39.5	35.0	41.0	25.0	41.0
20-21	35.35751274170639	39.0	35.0	41.0	25.0	41.0
22-23	35.329886189248846	39.0	35.0	41.0	25.0	41.0
24-25	35.237745593151686	39.0	35.0	41.0	25.0	41.0
26-27	35.16090522433758	39.0	34.5	41.0	24.5	41.0
28-29	35.022631533859254	39.0	34.0	41.0	24.0	41.0
30-31	34.92849388218298	39.0	34.0	41.0	24.0	41.0
32-33	34.83114066166975	39.0	34.0	41.0	24.0	41.0
34-35	34.72866326041732	39.0	34.0	41.0	23.5	41.0
36-37	34.59898328110441	39.0	34.0	41.0	23.0	41.0
38-39	34.45746229311583	39.0	34.0	41.0	22.0	41.0
40-41	34.34807941052355	39.0	33.5	41.0	21.0	41.0
42-43	34.26450939010086	39.0	33.0	41.0	20.5	41.0
44-45	34.109978588433634	38.0	33.0	41.0	19.5	41.0
46-47	33.88685991367686	38.0	33.0	40.0	18.0	41.0
48-49	33.75511638077369	38.0	33.0	40.0	17.5	41.0
50-51	33.590308397416436	38.0	33.0	40.0	15.5	41.0
52-53	33.3862885331517	38.0	32.5	40.0	14.5	41.0
54-55	33.13366126679901	38.0	32.0	40.0	13.0	41.0
56-57	32.873611738339974	37.5	32.0	40.0	9.0	41.0
58-59	32.64056780576129	37.0	32.0	40.0	8.0	41.0
60-61	32.337535972383364	36.5	31.0	40.0	7.5	41.0
62-63	32.059045342389396	36.0	31.0	39.5	7.0	41.0
64-65	31.738169170940342	36.0	31.0	39.0	4.0	41.0
66-67	31.45620993593981	35.0	30.5	39.0	2.0	40.5
68-69	31.099494509353086	35.0	30.0	38.5	2.0	40.0
70-71	30.775634520585033	35.0	30.0	38.0	2.0	40.0
72-73	30.418796674200465	35.0	30.0	37.0	2.0	39.5
74-75	30.352941036491146	35.0	30.0	37.0	2.0	39.0
76-77	30.09889589505001	35.0	30.0	36.0	2.0	39.0
78-79	29.83420618032251	34.5	30.0	36.0	2.0	38.5
80-81	29.486805631204938	34.0	29.0	35.5	2.0	37.0
82-83	29.234782399470344	34.0	29.0	35.0	2.0	37.0
84-85	29.049321297237384	34.0	29.0	35.0	2.0	36.5
86-87	28.836406960424824	34.0	29.0	35.0	2.0	36.0
88-89	28.681866772358404	34.0	29.0	35.0	2.0	36.0
90-91	28.53022909688562	34.0	29.0	35.0	2.0	35.0
92-93	28.713895678237407	34.0	29.0	35.0	2.0	35.0
94-95	28.701708033805843	34.0	29.0	35.0	2.0	35.0
96-97	28.645795765702786	34.0	29.0	35.0	2.0	35.0
98-99	28.54904142389825	34.0	29.0	35.0	2.0	35.0
100-101	28.44371941488096	34.0	29.0	35.0	2.0	35.0
102-103	27.121527957720488	32.5	26.0	34.0	2.0	35.0
104-105	28.143300834358346	34.0	29.0	35.0	2.0	35.0
106-107	28.31950813681827	34.0	29.0	35.0	2.0	35.0
108-109	28.26846370821466	34.0	29.0	35.0	2.0	35.0
110-111	28.2445535088211	34.0	29.0	35.0	2.0	35.0
112-113	28.164759993738876	34.0	29.0	35.0	2.0	35.0
114-115	28.043294303539756	34.0	29.0	35.0	2.0	35.0
116-117	27.8834839828369	34.0	29.0	35.0	2.0	35.0
118-119	27.765219451368935	34.0	29.0	35.0	2.0	35.0
120-121	27.586156964919724	34.0	29.0	35.0	2.0	35.0
122-123	27.432832249446598	34.0	29.0	35.0	2.0	35.0
124-125	26.35383314102232	33.0	25.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	153421.0
3	22203.0
4	14961.0
5	13361.0
6	15338.0
7	16938.0
8	17865.0
9	19849.0
10	21088.0
11	22289.0
12	23154.0
13	22788.0
14	22418.0
15	20581.0
16	17853.0
17	15937.0
18	15248.0
19	14519.0
20	14539.0
21	15624.0
22	18731.0
23	24505.0
24	33491.0
25	43247.0
26	51749.0
27	59983.0
28	71138.0
29	83087.0
30	99141.0
31	119183.0
32	147094.0
33	187375.0
34	249669.0
35	360469.0
36	603287.0
37	866803.0
38	263005.0
39	137.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.86691016391083	18.91356553416811	13.728603588109348	24.49092071381171
2	15.744875180276352	19.82074699856731	39.667857843601304	24.76651997755503
3	18.875534401351484	24.22089664652739	29.103908832174042	27.799660119947088
4	12.99720311676882	16.58409478937692	36.56575743279124	33.85294466106303
5	14.468246918725322	37.134195729728404	34.06790135372734	14.329655997818932
6	32.99767699831692	35.652879821352315	17.433697935741304	13.91574524458947
7	28.677899286145685	31.719845234607284	21.230475835888605	18.371779643358426
8	26.113396776070967	35.0842446539106	19.91261793709475	18.889740632923676
9	26.276776254155042	16.136418984470293	19.595414835441606	37.991389925933056
10-11	25.15738970028262	25.926449402596084	27.680701991910507	21.235458905210784
12-13	25.609915719871502	23.42745887995456	27.584243770084722	23.37838163008922
14-15	23.15448988266683	24.959975566883344	24.951143682723817	26.93439086772601
16-17	23.351510429275724	27.7429298598037	25.680530454051016	23.225029256869565
18-19	23.23082671199208	27.2265568293927	26.909131726195497	22.63348473241972
20-21	24.461041962620676	26.285767022886873	26.409763599957266	22.84342741453518
22-23	24.013492156457826	26.111140931505084	26.16238500161691	23.712981910420183
24-25	23.618094438426784	26.222059694740075	26.39519905804571	23.764646808787433
26-27	23.514969293553417	26.473726989996145	26.32917162664049	23.682132089809944
28-29	23.48467460102557	26.50091387567906	26.421914929174633	23.592496594120732
30-31	23.937961456618037	26.361066067664108	26.204343517743006	23.49662895797485
32-33	23.597532978518203	26.428333174024804	26.11624710352635	23.857886743930642
34-35	23.845805516314066	26.424959658573698	26.15212535358657	23.577109471525663
36-37	23.915222952513275	26.2838806285483	26.138157352844544	23.662739066093874
38-39	23.756434741429754	26.3442789268878	26.168605098507168	23.730681233175275
40-41	23.8483521995417	26.26965992966998	26.27621726077276	23.605770610015554
42-43	23.825339209545778	26.19225716996278	26.144081958145204	23.838321662346235
44-45	23.821099947117926	26.31612903225806	26.0353516657853	23.82741935483871
46-47	23.831246747697477	26.27492903105711	26.031852469211536	23.861971752033877
48-49	23.731997702263296	26.412589164545313	26.102817768194324	23.752595364997063
50-51	23.734843613164163	26.50393822794021	25.987822535950144	23.77339562294548
52-53	23.781226722991132	26.392627826722936	25.96297572355884	23.86316972672709
54-55	23.792846006169118	26.298715077587293	26.03375577074125	23.874683145502335
56-57	23.853541764140278	26.304291121657247	25.95374968287336	23.888417431329113
58-59	23.77357512741016	26.15382398392799	26.17089177000874	23.90170911865311
60-61	23.868419775770874	26.224913175037724	26.146301697524027	23.760365351667375
62-63	23.680338995778417	26.32358750436939	26.087931392804958	23.908142107047233
64-65	23.76197267860127	26.176194042005914	26.108147027031603	23.95368625236122
66-67	23.726692472845343	26.221020930503116	26.018141003268074	24.034145593383467
68-69	23.842572608786757	26.118697857415295	26.039030961813168	23.99969857198478
70-71	23.643961753419948	25.989350978143396	26.213767050168062	24.15292021826859
72-73	23.769073414492365	26.037609480807568	26.10782368105117	24.0854934236489
74-75	23.699619815596616	26.172318974145952	26.06186097469302	24.06620023556441
76-77	23.622815701355265	26.008463264861483	26.161332440682056	24.2073885931012
78-79	23.70992440486397	25.97299272842506	26.205710096004676	24.1113727707063
80-81	23.70751477136363	26.03704934170213	26.14557472512698	24.10986116180726
82-83	23.680922501209984	26.002524282035832	26.184461903158475	24.132091313595705
84-85	23.787481757609914	25.96058520014177	26.066043698712594	24.18588934353572
86-87	23.713505338360577	25.89423488786534	26.218132446338764	24.174127327435315
88-89	23.627007764006358	25.84013560834972	26.251193791333204	24.281662836310716
90-91	23.696982460649195	25.89696006531927	26.1855742610764	24.22048321295513
92-93	23.631252123537323	25.859958249768972	26.30457573542146	24.204213891272243
94-95	23.54628488268482	25.850798802972157	26.36667200770802	24.236244306635
96-97	23.580277532613334	25.739441814765673	26.391867144785806	24.288413507835184
98-99	23.542192012704284	25.7143978989067	26.250366535863932	24.493043552525084
100-101	23.499646420371477	25.851780530235917	26.319320016599534	24.329253032793073
102-103	23.391408594789674	25.72038152411337	26.400883238951778	24.487326642145177
104-105	23.256285903417947	25.882481571269945	26.291253854343587	24.56997867096852
106-107	23.299110059135923	25.77613174186757	26.360497112481475	24.564261086515028
108-109	23.273039015546562	25.78999447863273	26.34915190952573	24.587814596294972
110-111	23.31503275923959	25.865011175359605	26.240749175482563	24.579206889918247
112-113	23.208279068205563	25.871863391884364	26.38307824371495	24.536779296195117
114-115	23.20046707478419	25.991334102183977	26.282436134517223	24.52576268851461
116-117	23.143886001087786	25.912552640784604	26.312142699063273	24.63141865906434
118-119	23.2805778678761	25.843995345370395	26.216532290369145	24.65889449638436
120-121	23.185267300867086	25.86417904626675	26.330396338903906	24.62015731396226
122-123	23.193847231816182	25.875615987414193	26.21326467545602	24.717272105313604
124-125	23.142887726771548	25.959155881694084	26.17320358772377	24.724752803810595
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	462.0
1	349.0
2	206.5
3	153.5
4	123.0
5	117.5
6	122.5
7	128.5
8	154.5
9	187.0
10	226.5
11	256.0
12	250.5
13	268.5
14	312.5
15	374.5
16	436.5
17	494.5
18	586.0
19	701.5
20	889.0
21	1141.0
22	1446.5
23	1875.5
24	2449.5
25	3334.0
26	4840.0
27	6971.0
28	9891.5
29	14125.0
30	19463.0
31	25953.0
32	34351.0
33	43469.0
34	52901.5
35	62919.5
36	74401.0
37	85689.5
38	93876.0
39	101111.0
40	110075.0
41	119294.0
42	126683.0
43	134932.0
44	142884.5
45	148362.0
46	153392.0
47	158162.0
48	162626.0
49	165512.0
50	164796.0
51	161473.5
52	154878.5
53	148692.5
54	144192.0
55	137236.5
56	126732.0
57	113786.0
58	102561.5
59	92148.5
60	79500.0
61	66666.0
62	54589.5
63	41374.0
64	30213.5
65	22821.5
66	17156.0
67	13191.0
68	10031.5
69	7484.0
70	5902.5
71	4561.5
72	3358.0
73	2626.5
74	2091.0
75	1544.0
76	1128.0
77	749.5
78	495.5
79	359.0
80	254.5
81	176.0
82	125.5
83	95.5
84	69.5
85	44.5
86	31.5
87	25.5
88	21.5
89	21.0
90	16.5
91	12.5
92	15.0
93	14.0
94	13.5
95	13.0
96	9.0
97	6.0
98	7.0
99	12.0
100	26.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07128375269825926
2	0.009888769847607183
3	0.019407371839956344
4	0.018931441740338884
5	0.011501644074088569
6	0.008540301232024384
7	0.009095553014911419
8	0.007535559910609751
9	0.005790482878679072
10-11	0.009835888725427464
12-13	0.007271154299711163
14-15	0.008341997023850444
16-17	0.012625367920407566
18-19	0.006107769611757376
20-21	0.013537567278007692
22-23	0.0044155737020064156
24-25	0.0037942205163947343
26-27	0.003331510697322206
28-29	0.009835888725427464
30-31	0.005962346525763154
32-33	0.0026043952673510894
34-35	0.00754878019115468
36-37	0.006411836064290753
38-39	0.0015599931043016678
40-41	0.0010972832852291392
42-43	0.001057622443594351
44-45	0.0017979581541103966
46-47	0.0034901540638613586
48-49	0.0022606679731829254
50-51	0.004336252018736839
52-53	0.004521335946365851
54-55	0.004402353421461486
56-57	0.0016260945070263146
58-59	0.0023664302175423605
60-61	0.004693199593449933
62-63	0.0019566015206495496
64-65	0.0032786295751424882
66-67	7.403357105160458E-4
68-69	0.0018640595568350435
70-71	8.064371132406926E-4
72-73	0.001837618995745185
74-75	0.002313549095362643
76-77	0.0020094826428292667
78-79	3.305070136232347E-4
80-81	9.254196381450572E-5
82-83	6.610140272464693E-5
84-85	3.040664525333759E-4
86-87	2.1152448871887019E-4
88-89	0.0
90-91	7.799965521508339E-4
92-93	0.001401349737762515
94-95	8.989790770551983E-4
96-97	6.874545883363282E-4
98-99	9.783007603247748E-4
100-101	9.121993576001277E-4
102-103	0.0020888043260988433
104-105	0.003529814905496147
106-107	0.00495760520434852
108-109	0.010470462191584075
110-111	0.008209794218401149
112-113	0.002551514145171372
114-115	0.0061474304533921656
116-117	0.001771517593020538
118-119	7.13895149426187E-4
120-121	0.003714898833125158
122-123	8.064371132406926E-4
124-125	0.08560131652841778
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	3782068.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.85076659223501
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.80798515276942	34.79981574124916
2	14.260895324545475	14.218331284404243
3	6.1089109009520115	9.136016743683566
4	3.1476763931201655	6.276563247252863
5	1.7701392474720108	4.412139923074087
6	1.1567699745962203	3.4599522002701084
7	0.7851307013108173	2.7397557134810335
8	0.541217416438332	2.15840824820158
9	0.3930403844861368	1.7634028021506643
>10	1.9273925562615528	16.462138201131808
>50	0.07760351191900806	2.6111373542687515
>100	0.022759355001676123	1.7542948963450105
>500	3.7261865703848337E-4	0.12564880949073878
>1k	1.0646247011548717E-4	0.08239483499639316
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.6440561089858777E-5
2	0.0	0.0	0.0	0.0	2.6440561089858777E-5
3	0.0	0.0	0.0	0.0	2.6440561089858777E-5
4	0.0	0.0	0.0	0.0	2.6440561089858777E-5
5	0.0	0.0	0.0	0.0	2.6440561089858777E-5
6	0.0	0.0	0.0	0.0	7.932168326957633E-5
7	0.0	0.0	0.0	2.6440561089858777E-5	7.932168326957633E-5
8	0.0	0.0	0.0	2.6440561089858777E-5	1.057622443594351E-4
9	0.0	0.0	0.0	5.288112217971755E-5	1.5864336653915266E-4
10-11	0.0	0.0	0.0	5.288112217971755E-5	1.5864336653915266E-4
12-13	0.0	0.0	0.0	5.288112217971755E-5	1.7186364708408203E-4
14-15	0.0	0.0	0.0	5.288112217971755E-5	2.3796504980872898E-4
16-17	0.0	0.0	0.0	5.288112217971755E-5	3.172867330783053E-4
18-19	0.0	0.0	0.0	1.057622443594351E-4	3.8338813580295224E-4
20-21	0.0	0.0	0.0	1.4542308599422326E-4	3.9660841634788164E-4
22-23	0.0	0.0	0.0	2.247447692637996E-4	4.362692579826698E-4
24-25	0.0	0.0	0.0	4.362692579826698E-4	5.023706607073167E-4
26-27	0.0	0.0	0.0	8.460979548754809E-4	5.684720634319637E-4
28-29	0.0	0.0	0.0	0.002181346289913349	5.816923439768931E-4
30-31	0.0	0.0	0.0	0.007747084399328622	5.949126245218225E-4
32-33	0.0	0.0	0.0	0.021192109713521808	6.081329050667518E-4
34-35	0.0	0.0	0.0	0.040467278748028856	7.271154299711163E-4
36-37	0.0	0.0	0.0	0.07298916888855515	7.403357105160457E-4
38-39	0.0	0.0	0.0	0.12593639247099736	7.667762716059045E-4
40-41	0.0	0.0	0.0	0.18455511640721425	8.196573937856221E-4
42-43	0.0	0.0	0.0	0.24277723192708328	8.328776743305515E-4
44-45	0.0	0.0	0.0	0.3130562433039279	8.460979548754809E-4
46-47	0.0	0.0	0.0	0.39667451775060625	9.254196381450571E-4
48-49	0.0	0.0	0.0	0.48366396373624165	9.650804797798453E-4
50-51	0.0	0.0	0.0	0.572900857414515	9.783007603247748E-4
52-53	0.0	0.0	0.0	0.6652709575819367	0.0010047413214146336
54-55	0.0	0.0	0.0	0.7701871039864963	0.0010444021630494215
56-57	0.0	0.0	0.0	0.8931621536154295	0.001057622443594351
58-59	0.0	0.0	0.0	1.0284452844316918	0.0010708427241392805
60-61	0.0	0.0	0.0	1.169056188307561	0.0010840630046842097
62-63	0.0	0.0	0.0	1.3131175854056565	0.0011501644074088566
64-65	0.0	0.0	0.0	1.4746561933841487	0.001189825249043645
66-67	0.0	0.0	0.0	1.6581536873477685	0.0012427063712233625
68-69	0.0	0.0	0.0	1.8643107421653973	0.0012427063712233625
70-71	0.0	0.0	0.0	2.086239591673127	0.00129558749340308
72-73	0.0	0.0	0.0	2.3134962142404634	0.0013881294572175857
74-75	0.0	0.0	0.0	2.5562734461675465	0.0014277902988523738
76-77	0.0	0.0	0.0	2.8347586558464837	0.0015071119821219502
78-79	0.0	0.0	0.0	3.141918654027373	0.0015203322626668797
80-81	0.0	0.0	0.0	3.4641233314683926	0.0015467728237567382
82-83	0.0	0.0	0.0	3.8083662165778085	0.0015864336653915265
84-85	0.0	0.0	0.0	4.167521578141905	0.0016128742264813853
86-87	0.0	0.0	0.0	4.559093067602168	0.0016393147875712441
88-89	0.0	0.0	0.0	4.986412195655921	0.0016789756292060324
90-91	0.0	0.0	0.0	5.4415335736956605	0.0016921959097509617
92-93	0.0	0.0	0.0	5.915004701131762	0.0017979581541103966
94-95	0.0	2.6440561089858777E-5	0.0	6.403282542778184	0.0018508392762901142
96-97	0.0	2.6440561089858777E-5	0.0	6.92356668362388	0.0018640595568350437
98-99	0.0	2.6440561089858777E-5	0.0	7.473239508120955	0.0019301609595596906
100-101	2.6440561089858777E-5	2.6440561089858777E-5	0.0	8.072210758770069	0.0019301609595596906
102-103	2.6440561089858777E-5	2.6440561089858777E-5	0.0	8.676351139112253	0.001956601520649549
104-105	2.6440561089858777E-5	2.6440561089858777E-5	0.0	9.297241614904863	0.0019962623622843375
106-107	2.6440561089858777E-5	3.9660841634788165E-5	0.0	9.931815081061472	0.0020094826428292667
108-109	2.6440561089858777E-5	5.288112217971755E-5	0.0	10.60403990621004	0.0020359232039191258
110-111	2.6440561089858777E-5	5.288112217971755E-5	0.0	11.304199184150047	0.002075584045553914
112-113	2.6440561089858777E-5	5.288112217971755E-5	0.0	12.026859379577523	0.0020888043260988433
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	8080	0.0	36.11021	1
GGTATCA	5750	0.0	31.274067	1
TCAACGC	9280	0.0	30.650936	4
CAACGCA	9700	0.0	29.323395	5
ATCAACG	9935	0.0	28.691576	3
AACGCAG	9910	0.0	28.520365	6
TATCAAC	10135	0.0	28.360258	2
GTACATG	15565	0.0	26.511204	1
TACATGG	15610	0.0	25.847204	2
ACATGGG	15655	0.0	25.31675	3
CATGGGG	10485	0.0	24.914944	4
ACGCAGA	11680	0.0	23.892693	7
CGCAGAG	11955	0.0	23.04385	8
GCAGAGT	13600	0.0	19.337797	9
AGAGTAC	11570	0.0	18.463596	10-11
CCGTATG	530	3.6379788E-12	17.96254	9
GTATAGG	1150	0.0	16.569044	1
CAGAGTA	12840	0.0	16.567854	10-11
ACCGTAT	510	5.5479177E-10	16.333584	8
TACCGTA	330	7.5114367E-6	16.227951	7
>>END_MODULE