FastQCFastQC Report
Mon 15 Aug 2016
SRR2921132_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921132_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3782068
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA99300.041.27781
GGTATCA65350.037.7975651
TCAACGC119800.033.707444
ATCAACG124150.032.429013
CAACGCA124750.032.3547025
TATCAAC128050.031.9045832
AACGCAG129300.030.9638236
ACGCAGA147400.026.9194647
CGCAGAG152300.025.7021718
GTACATG157700.025.0079941
TACATGG157550.024.3407882
CATGGGG102200.023.8707644
ACATGGG158400.023.383693
GCAGAGT170400.021.8903779
AGAGTAC147400.020.6443210-11
TACCGTA3452.8987415E-818.9675677
CAGAGTA160600.018.5030110-11
GAGTACT65550.018.4235312-13
GTATAAT17750.018.1511631
AGTACTT75950.017.54566412-13
Produced by FastQC (version 0.11.5)