##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921132_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3782068
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.68895271052768	31.0	31.0	34.0	27.0	34.0
2	30.850175089395538	31.0	31.0	34.0	27.0	34.0
3	30.92746640197902	31.0	31.0	34.0	27.0	34.0
4	34.43765157051645	37.0	35.0	37.0	31.0	37.0
5	34.10541349335866	37.0	35.0	37.0	30.0	37.0
6	33.96929404759513	37.0	35.0	37.0	29.0	37.0
7	33.78534204038637	37.0	35.0	37.0	28.0	37.0
8	33.7814415288144	37.0	35.0	37.0	28.0	37.0
9	35.2160061638236	39.0	35.0	39.0	28.0	39.0
10-11	35.16500456892895	39.0	35.0	39.0	28.0	39.0
12-13	35.05379080968402	39.0	35.0	39.0	27.0	39.0
14-15	36.15793211544583	40.0	36.0	41.0	27.0	41.0
16-17	36.10462873750551	40.0	36.0	41.0	27.0	41.0
18-19	36.135181070250454	40.0	36.0	41.0	27.0	41.0
20-21	36.12296037511753	40.0	36.0	41.0	27.0	41.0
22-23	36.086240649295576	40.0	36.0	41.0	27.0	41.0
24-25	35.99184454113464	40.0	36.0	41.0	27.0	41.0
26-27	35.88617708618671	40.0	35.5	41.0	27.0	41.0
28-29	35.795122271730705	40.0	35.0	41.0	27.0	41.0
30-31	35.718429573450294	39.0	35.0	41.0	27.0	41.0
32-33	35.60060580613569	39.0	35.0	41.0	26.0	41.0
34-35	35.488949564100906	39.0	35.0	41.0	26.0	41.0
36-37	35.352579065209824	39.0	35.0	41.0	25.0	41.0
38-39	35.194568817905974	39.0	35.0	41.0	25.0	41.0
40-41	35.06353561067649	39.0	34.5	41.0	24.5	41.0
42-43	34.942493498266025	39.0	34.0	41.0	24.0	41.0
44-45	34.78933879560071	39.0	34.0	41.0	24.0	41.0
46-47	34.595016800332516	39.0	34.0	41.0	23.0	41.0
48-49	34.411611319521484	39.0	33.5	41.0	22.5	41.0
50-51	34.211381709688986	38.0	33.0	41.0	22.0	41.0
52-53	34.042628530211516	38.0	33.0	40.0	21.0	41.0
54-55	33.832655044806174	38.0	33.0	40.0	20.0	41.0
56-57	33.56913016899749	38.0	33.0	40.0	19.0	41.0
58-59	33.343918723830456	37.5	32.5	40.0	18.0	41.0
60-61	33.07812789193637	37.0	32.0	40.0	17.5	41.0
62-63	32.78482724266195	36.5	32.0	40.0	15.0	41.0
64-65	32.48353559481215	36.0	32.0	39.0	13.0	41.0
66-67	32.15217270022644	35.5	31.0	39.0	11.5	41.0
68-69	31.790286425310175	35.0	31.0	39.0	8.0	40.0
70-71	31.429608880644132	35.0	31.0	38.0	8.0	40.0
72-73	31.224832948535035	35.0	31.0	37.0	7.0	39.5
74-75	31.021282933040865	35.0	31.0	37.0	7.0	39.0
76-77	30.777759945088242	35.0	31.0	36.5	6.0	39.0
78-79	30.451627257891715	35.0	30.0	36.0	2.0	38.5
80-81	30.152708518196924	35.0	30.0	35.5	2.0	37.0
82-83	29.92377384542002	35.0	30.0	35.0	2.0	37.0
84-85	29.756916718578303	34.0	30.0	35.0	2.0	36.5
86-87	29.551565043251472	34.0	30.0	35.0	2.0	36.0
88-89	29.402546305354637	34.0	30.0	35.0	2.0	36.0
90-91	29.245577155143692	34.0	30.0	35.0	2.0	35.5
92-93	29.128227467089435	34.0	29.5	35.0	2.0	35.0
94-95	29.028245922601073	34.0	29.0	35.0	2.0	35.0
96-97	28.95905044541769	34.0	29.0	35.0	2.0	35.0
98-99	28.91730635197463	34.0	29.0	35.0	2.0	35.0
100-101	28.777586495007494	34.0	29.0	35.0	2.0	35.0
102-103	27.401585455364632	32.5	26.0	34.0	2.0	35.0
104-105	28.501462030825465	34.0	29.0	35.0	2.0	35.0
106-107	28.787105625811066	34.0	29.0	35.0	2.0	35.0
108-109	28.992450029983594	34.0	30.0	35.0	2.0	35.0
110-111	28.979999037563577	34.0	30.0	35.0	2.0	35.0
112-113	28.90906033418754	34.0	30.0	35.0	2.0	35.0
114-115	28.799358710631324	34.0	30.0	35.0	2.0	35.0
116-117	28.714009901461317	34.0	30.0	35.0	2.0	35.0
118-119	28.578042621126855	34.0	30.0	35.0	2.0	35.0
120-121	28.42108219630107	34.0	29.5	35.0	2.0	35.0
122-123	28.243080240757173	34.0	29.0	35.0	2.0	35.0
124-125	27.443576635851073	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	83961.0
3	29464.0
4	15005.0
5	12103.0
6	12954.0
7	15384.0
8	18094.0
9	19807.0
10	21261.0
11	22674.0
12	23668.0
13	24689.0
14	25922.0
15	22612.0
16	17807.0
17	16523.0
18	15882.0
19	15122.0
20	15305.0
21	15556.0
22	17356.0
23	21228.0
24	28582.0
25	38244.0
26	48066.0
27	57349.0
28	68079.0
29	80960.0
30	95290.0
31	115823.0
32	142489.0
33	179709.0
34	236487.0
35	340323.0
36	593295.0
37	944285.0
38	330390.0
39	320.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.495062096236865	19.319558396182828	14.466859338673888	24.718520168906423
2	15.927607744135342	20.348370809362724	38.55460328254799	25.16941816395395
3	18.631246266894657	24.306042322673036	29.69612423351531	27.366587176917
4	13.380077790872724	16.99518383212896	36.09778879060173	33.52694958639658
5	14.254726399226112	37.08419183474861	34.27527486818525	14.385806897840029
6	32.837639156992886	36.045905280693866	17.09553904328741	14.020916519025835
7	28.496690268819137	31.603833957059173	21.27771595757959	18.621759816542095
8	26.162178657095588	35.05230098139208	19.88793836795341	18.89758199355893
9	26.228690991811987	15.65633940183774	19.542696294635135	38.572273311715136
10-11	25.262492309388872	25.712590573350347	27.780517065586746	21.24440005167403
12-13	25.836165122521187	23.108497271657782	27.77451980793507	23.280817797885952
14-15	23.274704195239273	24.598676543377053	25.157762318658133	26.968856942725544
16-17	23.311016763346238	27.568276723352025	25.870969072852045	23.249737440449696
18-19	23.241338846470143	26.80017629554814	27.355695045727487	22.602789812254233
20-21	24.31353869736989	25.845199941392067	26.971842272718376	22.869419088519667
22-23	23.9257463431161	25.76374598633081	26.700300754922228	23.610206915630858
24-25	23.53832146831355	25.942996332389818	26.948134424822584	23.57054777447404
26-27	23.44886618643465	26.158693963564467	26.89303266863065	23.499407181370234
28-29	23.38625280375426	26.105679551219474	27.017255072676114	23.49081257235015
30-31	23.81860976166765	25.980145119701735	26.841683426624446	23.35956169200617
32-33	23.48605971489342	26.05335450817098	26.762332502692587	23.69825327424302
34-35	23.658879461834047	26.06729428186775	26.757324546561833	23.516501709736367
36-37	23.83044144973793	25.95394338386085	26.756592494873104	23.459022671528114
38-39	23.64762901892048	26.085651892345386	26.66117054879583	23.605548539938304
40-41	23.734797275182956	25.935021050511576	26.82791396881983	23.502267705485643
42-43	23.790929068667396	25.874756646547777	26.628639920994928	23.705674363789907
44-45	23.74568702635311	26.04279675877022	26.4883230354448	23.72319317943187
46-47	23.84075010823853	25.91886381410637	26.441542861268747	23.79884321638635
48-49	23.7607192492261	26.112930803072597	26.397512406890467	23.728837540810837
50-51	23.71578273766663	26.21953082196808	26.305025626621376	23.759660813743917
52-53	23.818178476165215	26.150487713827303	26.297840592035115	23.73349321797237
54-55	23.761714503271474	26.124735796766075	26.3086052462831	23.80494445367935
56-57	23.813705064535686	26.163992914661875	26.181355827981534	23.840946192820905
58-59	23.649718855895166	26.060674021205678	26.43721220779777	23.85239491510138
60-61	23.68447847917474	26.266223762630236	26.33953566483872	23.709762093356307
62-63	23.56715323274055	26.31394924129599	26.26771792656256	23.851179599400897
64-65	23.59539625506006	26.17831775255995	26.319860559902775	23.906425432477217
66-67	23.562556328235498	26.215807149313548	26.238764934627838	23.982871587823116
68-69	23.639929749589104	26.05211971133381	26.2928467800771	24.01510375899999
70-71	23.497670075865532	25.89258213255341	26.40068876088505	24.209059030696015
72-73	23.602595943675993	25.959648367100325	26.313812617367578	24.123943071856104
74-75	23.582037999743196	26.03995737224401	26.23701619460663	24.140988433406164
76-77	23.51641318978899	25.89818348182292	26.29382955035291	24.29157377803518
78-79	23.511935419162814	25.868447314515812	26.325717280192467	24.29389998612891
80-81	23.489391874317086	25.94397263572613	26.28834017938267	24.278295310574112
82-83	23.440448411826296	25.87318941480236	26.306005526865118	24.380356646506225
84-85	23.524689777038258	25.891080723751987	26.173354628608436	24.410874870601322
86-87	23.426400610558147	25.800405557856255	26.26002986149787	24.513163970087728
88-89	23.33344396171976	25.751419073506217	26.346657090038857	24.568479874735168
90-91	23.360324791349406	25.810368512142983	26.301075413840152	24.52823128266746
92-93	23.28767902186636	25.76825286506391	26.335143320633804	24.608924792435925
94-95	23.244238432172327	25.701955553392942	26.39161419101103	24.662191823423694
96-97	23.25864581322346	25.59103855542127	26.424083188101477	24.726232443253792
98-99	23.176280962121524	25.56804493532217	26.25513859156643	25.000535510989874
100-101	23.140976581652165	25.626316644073587	26.384039854085557	24.84866692018869
102-103	23.000670105873557	25.501058841325065	26.41802494888261	25.080246103918768
104-105	22.86395061091751	25.63936264388676	26.284903550997278	25.21178319419845
106-107	22.826753101720076	25.5578190663179	26.360359757614095	25.255068074347932
108-109	22.790505677046202	25.525711190984424	26.41029695806792	25.273486173901453
110-111	22.76478478540683	25.62980405949018	26.243758482613	25.36165267248999
112-113	22.616337037183452	25.62759217393362	26.380600976466773	25.375469812416153
114-115	22.57990604359776	25.69584113615005	26.297955922040405	25.42629689821178
116-117	22.4867918641056	25.606265336774413	26.322624915383315	25.584317883736674
118-119	22.519900842835895	25.533326722855726	26.239788464716575	25.7069839695918
120-121	22.35656021352941	25.576595636946596	26.325466295730404	25.741377853793594
122-123	22.31774387217539	25.62041108919625	26.215668858828593	25.846176179799762
124-125	22.21860518150122	25.64160291136357	26.199600153123843	25.940191754011366
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	27.0
1	50.5
2	125.5
3	188.5
4	157.5
5	113.5
6	137.0
7	320.0
8	493.5
9	545.5
10	507.0
11	396.5
12	403.0
13	492.0
14	558.5
15	625.0
16	741.0
17	848.5
18	964.0
19	1110.5
20	1298.5
21	1544.5
22	1848.0
23	2267.5
24	2859.5
25	3770.5
26	5241.0
27	7373.0
28	10334.0
29	14466.0
30	19910.5
31	26425.0
32	34427.5
33	43160.0
34	52394.5
35	62326.5
36	73555.0
37	84330.0
38	92620.5
39	99950.0
40	108987.0
41	118613.5
42	126219.0
43	134563.0
44	142965.5
45	149039.5
46	153827.0
47	159004.0
48	164612.0
49	167803.5
50	167010.5
51	162999.0
52	156264.0
53	150048.0
54	144883.0
55	137881.0
56	127148.0
57	113927.0
58	102280.0
59	91599.0
60	78869.5
61	65786.5
62	53424.0
63	40125.5
64	29273.5
65	21921.0
66	16036.0
67	12101.0
68	9224.0
69	6875.5
70	5235.5
71	3954.5
72	2926.5
73	2289.0
74	1776.5
75	1283.5
76	923.5
77	575.0
78	352.0
79	208.5
80	128.0
81	75.5
82	38.0
83	26.0
84	14.5
85	9.0
86	9.5
87	8.5
88	5.0
89	2.0
90	1.5
91	3.0
92	4.5
93	3.0
94	3.5
95	4.5
96	2.5
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07757660623764565
2	1.5864336653915266E-4
3	7.667762716059045E-4
4	0.18706696971075085
5	0.07168036111460714
6	0.00973012648106803
7	0.013008756056210517
8	0.010549783874853651
9	0.01813822490764312
10-11	0.017754836771840168
12-13	0.01963211660922014
14-15	0.026030732392965966
16-17	0.02699581287274581
18-19	0.024259214799945427
20-21	0.026704966700757362
22-23	0.028621907379772122
24-25	0.026321578564954408
26-27	0.025885309306971742
28-29	0.025832428184792026
30-31	0.025977851270786244
32-33	0.02248769720692489
34-35	0.02457650153302373
36-37	0.027128015678195103
38-39	0.03219138312690306
40-41	0.02759072549726763
42-43	0.02763038633890242
44-45	0.027339540166913975
46-47	0.028212078682879317
48-49	0.023875826664142476
50-51	0.030049697678624497
52-53	0.026665305859122572
54-55	0.02973241094554619
56-57	0.02698259259220088
58-59	0.024087351152861345
60-61	0.025462260329533998
62-63	0.02858224653813734
64-65	0.03260121182379587
66-67	0.03213850200472334
68-69	0.0270354737143806
70-71	0.036104586168202155
72-73	0.03906592901026634
74-75	0.02547548061007893
76-77	0.017397889197127075
78-79	0.02174736149640884
80-81	0.024682263777383168
82-83	0.023822945541962758
84-85	0.016776536011515394
86-87	0.016128742264813853
88-89	0.016908738816964686
90-91	0.01815144518818805
92-93	0.02183990346022335
94-95	0.016710434608790747
96-97	0.020041945306112953
98-99	0.016736875169880604
100-101	0.017820938174564815
102-103	0.015229763187758654
104-105	0.017596193405301015
106-107	0.015269424029393443
108-109	0.013854854011085998
110-111	0.011514864354633498
112-113	0.009849109005972394
114-115	0.008302336182215656
116-117	0.008143692815676503
118-119	0.005103028290342744
120-121	0.0028159197560699597
122-123	0.0069009864444531405
124-125	0.05608043007159047
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	3782068.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.64362839922256
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.60960990955837	33.37420369056175
2	14.860310172960514	14.457188119013557
3	6.341955977636797	9.254872497011775
4	3.230735684565416	6.286188243844319
5	1.876636878038684	4.564321346779544
6	1.1945586663554137	3.4864600720358165
7	0.7913178331591976	2.6944799435311797
8	0.5551994525874251	2.1605532687291586
9	0.4345816695430796	1.9025666318130567
>10	1.9925015382651516	16.64355955406707
>50	0.08719269406196942	2.838928996108255
>100	0.024636287179511843	1.8458378452550908
>500	3.2710120736308026E-4	0.09671472740575393
>1k	4.3613488112571923E-4	0.39412506384367385
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	2.6440561089858777E-5
3	0.0	0.0	0.0	0.0	2.6440561089858777E-5
4	0.0	0.0	0.0	0.0	2.6440561089858777E-5
5	0.0	0.0	0.0	0.0	2.6440561089858777E-5
6	0.0	0.0	0.0	0.0	5.288112217971755E-5
7	0.0	0.0	0.0	0.0	5.288112217971755E-5
8	0.0	0.0	0.0	0.0	7.932168326957633E-5
9	0.0	0.0	0.0	0.0	7.932168326957633E-5
10-11	0.0	0.0	0.0	0.0	7.932168326957633E-5
12-13	0.0	0.0	0.0	0.0	7.932168326957633E-5
14-15	0.0	0.0	0.0	1.3220280544929388E-5	7.932168326957633E-5
16-17	0.0	0.0	0.0	5.288112217971755E-5	7.932168326957633E-5
18-19	0.0	0.0	0.0	1.057622443594351E-4	1.057622443594351E-4
20-21	0.0	0.0	0.0	1.4542308599422326E-4	1.5864336653915263E-4
22-23	0.0	0.0	0.0	2.247447692637996E-4	2.9084617198844653E-4
24-25	0.0	0.0	0.0	4.6270981907252856E-4	4.362692579826698E-4
26-27	0.0	0.0	0.0	8.725385159653396E-4	4.6270981907252856E-4
28-29	0.0	0.0	0.0	0.002115244887188702	4.7593009961745795E-4
30-31	0.0	0.0	0.0	0.007495899068974963	5.023706607073167E-4
32-33	0.0	0.0	0.0	0.020821941858263786	5.816923439768931E-4
34-35	0.0	0.0	0.0	0.039806264720782386	6.345734661566106E-4
36-37	0.0	0.0	0.0	0.07185222476169123	6.345734661566106E-4
38-39	0.0	0.0	0.0	0.12383436786435359	6.345734661566106E-4
40-41	0.0	0.0	0.0	0.181421909918066	6.345734661566106E-4
42-43	0.0	0.0	0.0	0.23911521421613785	6.742343077913988E-4
44-45	0.0	0.0	0.0	0.3091430402626288	7.138951494261869E-4
46-47	0.0	0.0	0.0	0.39162437058244326	7.932168326957633E-4
48-49	1.3220280544929388E-5	0.0	0.0	0.47698772206105233	8.725385159653396E-4
50-51	2.6440561089858777E-5	0.0	0.0	0.5647439443182936	8.725385159653396E-4
52-53	2.6440561089858777E-5	0.0	0.0	0.6558316772728571	9.650804797798453E-4
54-55	2.6440561089858777E-5	0.0	0.0	0.7601661313334398	0.0010444021630494215
56-57	2.6440561089858777E-5	0.0	0.0	0.8815283067358916	0.001057622443594351
58-59	2.6440561089858777E-5	0.0	0.0	1.0148019549093248	0.001057622443594351
60-61	2.6440561089858777E-5	0.0	0.0	1.153509138386724	0.001057622443594351
62-63	2.6440561089858777E-5	0.0	0.0	1.295759357050164	0.0010708427241392805
64-65	2.6440561089858777E-5	0.0	0.0	1.4554471257523662	0.0010840630046842097
66-67	2.6440561089858777E-5	0.0	0.0	1.6371731021229654	0.0010840630046842097
68-69	2.6440561089858777E-5	0.0	0.0	1.841704062433568	0.0010840630046842097
70-71	2.6440561089858777E-5	0.0	0.0	2.0619671565926367	0.001123723846318998
72-73	2.6440561089858777E-5	0.0	0.0	2.288747849060355	0.0012427063712233625
74-75	2.6440561089858777E-5	0.0	0.0	2.529912206760957	0.0012427063712233625
76-77	2.6440561089858777E-5	0.0	0.0	2.80653335688306	0.0012691469323132213
78-79	2.6440561089858777E-5	0.0	0.0	3.1130191207561575	0.0012823672128581508
80-81	2.6440561089858777E-5	0.0	0.0	3.4346024450115653	0.0013484686155827976
82-83	2.6440561089858777E-5	2.6440561089858777E-5	0.0	3.775725343912378	0.0013749091766726564
84-85	2.6440561089858777E-5	2.6440561089858777E-5	0.0	4.131654957023512	0.0013749091766726564
86-87	2.6440561089858777E-5	2.6440561089858777E-5	0.0	4.5205559498137	0.0013881294572175857
88-89	2.6440561089858777E-5	2.6440561089858777E-5	0.0	4.942256458635857	0.001401349737762515
90-91	2.6440561089858777E-5	2.6440561089858777E-5	0.0	5.390635493597682	0.0014145700183074445
92-93	2.6440561089858777E-5	2.6440561089858777E-5	0.0	5.859135795548891	0.001467451140487162
94-95	2.6440561089858777E-5	2.6440561089858777E-5	0.0	6.341543832633364	0.0015599931043016678
96-97	2.6440561089858777E-5	3.9660841634788165E-5	0.0	6.856830707433076	0.0015864336653915265
98-99	2.6440561089858777E-5	5.288112217971755E-5	0.0	7.401241860273268	0.0016128742264813853
100-101	2.6440561089858777E-5	5.288112217971755E-5	0.0	7.995096862351497	0.0016128742264813853
102-103	2.6440561089858777E-5	5.288112217971755E-5	0.0	8.59454404310023	0.001665755348661103
104-105	2.6440561089858777E-5	5.288112217971755E-5	0.0	9.21401994887453	0.00173185675138575
106-107	2.6440561089858777E-5	5.288112217971755E-5	0.0	9.848091044370435	0.0017450770319306793
108-109	2.6440561089858777E-5	5.288112217971755E-5	0.0	10.51909960370887	0.0017847378735654674
110-111	2.6440561089858777E-5	6.610140272464695E-5	0.0	11.217791430508388	0.001877279837379973
112-113	2.6440561089858777E-5	7.932168326957633E-5	0.0	11.939327902089545	0.0019301609595596906
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	9930	0.0	41.2778	1
GGTATCA	6535	0.0	37.797565	1
TCAACGC	11980	0.0	33.70744	4
ATCAACG	12415	0.0	32.42901	3
CAACGCA	12475	0.0	32.354702	5
TATCAAC	12805	0.0	31.904583	2
AACGCAG	12930	0.0	30.963823	6
ACGCAGA	14740	0.0	26.919464	7
CGCAGAG	15230	0.0	25.702171	8
GTACATG	15770	0.0	25.007994	1
TACATGG	15755	0.0	24.340788	2
CATGGGG	10220	0.0	23.870764	4
ACATGGG	15840	0.0	23.38369	3
GCAGAGT	17040	0.0	21.890377	9
AGAGTAC	14740	0.0	20.64432	10-11
TACCGTA	345	2.8987415E-8	18.967567	7
CAGAGTA	16060	0.0	18.50301	10-11
GAGTACT	6555	0.0	18.42353	12-13
GTATAAT	1775	0.0	18.151163	1
AGTACTT	7595	0.0	17.545664	12-13
>>END_MODULE