FastQCFastQC Report
Mon 15 Aug 2016
SRR2921131_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921131_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2777022
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA38210.13759343642218175No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT36140.13013940833021848No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA120700.026.636771
GTACATG167900.024.042191
AACGCAG133200.023.6755856
CATGGGG89100.023.5761554
TCAACGC135600.023.4784854
TACATGG168850.023.4371282
CAACGCA136500.023.106125
TATCAAC138600.023.0136432
ATCAACG139750.022.909433
GTATCGC1300.002052554422.8852546
GGTATCA94750.021.6787831
ACATGGG176550.021.6396943
ACGCAGA148350.021.0572097
CGCAGAG152800.020.1324338
GCAGAGT161700.018.2147929
ATGGGGC50950.017.9870665
GTATAGT9000.017.8614331
GTATAGG6950.017.133271
TACACCG3609.363375E-716.5303235
CAGAGTA150100.016.15690210-11
Produced by FastQC (version 0.11.5)