##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921131_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2777022
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.44505913168855	31.0	30.0	34.0	25.0	34.0
2	29.51960589437174	31.0	30.0	34.0	25.0	34.0
3	29.587660450655413	31.0	30.0	34.0	25.0	34.0
4	33.000055815186194	35.0	35.0	37.0	26.0	37.0
5	33.08868672988547	35.0	35.0	37.0	26.0	37.0
6	33.12339297276003	37.0	35.0	37.0	26.0	37.0
7	33.106850071767525	37.0	35.0	37.0	26.0	37.0
8	33.0594489348662	37.0	35.0	37.0	26.0	37.0
9	34.45390349806375	39.0	35.0	39.0	26.0	39.0
10-11	34.40717736481742	39.0	35.0	39.0	26.0	39.0
12-13	34.38748594717651	39.0	35.0	39.0	26.0	39.0
14-15	35.417365976935	40.0	35.0	41.0	25.0	41.0
16-17	35.32420863068424	39.0	35.0	41.0	25.0	41.0
18-19	35.40037763474686	39.5	35.0	41.0	25.0	41.0
20-21	35.430388380070454	40.0	35.0	41.0	25.0	41.0
22-23	35.41044003252405	40.0	35.0	41.0	25.0	41.0
24-25	35.3180291693764	39.5	35.0	41.0	25.0	41.0
26-27	35.2396722100149	39.0	35.0	41.0	25.0	41.0
28-29	35.0843756369233	39.0	34.5	41.0	24.0	41.0
30-31	34.970799655170175	39.0	34.0	41.0	24.0	41.0
32-33	34.857340885308076	39.0	34.0	41.0	24.0	41.0
34-35	34.74904808100188	39.0	34.0	41.0	23.5	41.0
36-37	34.61060787419041	39.0	34.0	41.0	23.0	41.0
38-39	34.443110101396385	39.0	34.0	41.0	21.5	41.0
40-41	34.32541117787328	39.0	33.5	41.0	20.5	41.0
42-43	34.23826926830252	39.0	33.0	41.0	20.0	41.0
44-45	34.07876170948592	38.5	33.0	41.0	18.5	41.0
46-47	33.84548736020096	38.0	33.0	40.5	17.5	41.0
48-49	33.70640329820938	38.0	33.0	40.0	15.5	41.0
50-51	33.53480346932793	38.0	33.0	40.0	14.5	41.0
52-53	33.3216782222107	38.0	32.5	40.0	12.0	41.0
54-55	33.06432916267858	38.0	32.0	40.0	9.0	41.0
56-57	32.788522201120486	37.5	32.0	40.0	8.0	41.0
58-59	32.55476297991157	37.0	31.5	40.0	7.5	41.0
60-61	32.24793825904152	36.5	31.0	40.0	6.5	41.0
62-63	31.970497893066746	36.0	31.0	39.5	2.0	41.0
64-65	31.648483699444945	35.5	31.0	39.0	2.0	41.0
66-67	31.357714667006597	35.0	30.5	39.0	2.0	40.5
68-69	30.991740072638965	35.0	30.0	38.5	2.0	40.0
70-71	30.663278144717616	35.0	30.0	37.5	2.0	40.0
72-73	30.300744106456484	35.0	29.5	37.0	2.0	39.0
74-75	30.22765303983908	35.0	30.0	37.0	2.0	39.0
76-77	29.972504178937005	35.0	30.0	36.0	2.0	39.0
78-79	29.716306172583437	34.5	29.5	36.0	2.0	38.5
80-81	29.367707565874525	34.0	29.0	35.5	2.0	37.0
82-83	29.116413733848706	34.0	29.0	35.0	2.0	37.0
84-85	28.92857942789074	34.0	29.0	35.0	2.0	36.0
86-87	28.716756655150732	34.0	29.0	35.0	2.0	36.0
88-89	28.55469384110029	34.0	29.0	35.0	2.0	36.0
90-91	28.391594665076475	34.0	29.0	35.0	2.0	35.0
92-93	28.571873395313396	34.0	29.0	35.0	2.0	35.0
94-95	28.544944008365796	34.0	29.0	35.0	2.0	35.0
96-97	28.482026249702017	34.0	29.0	35.0	2.0	35.0
98-99	28.363616672824342	34.0	29.0	35.0	2.0	35.0
100-101	28.250981627081096	34.0	29.0	35.0	2.0	35.0
102-103	26.930609120129404	32.5	26.0	34.5	2.0	35.0
104-105	27.937192791414688	34.0	28.0	35.0	2.0	35.0
106-107	28.089662955496934	34.0	29.0	35.0	2.0	35.0
108-109	28.020528825482838	34.0	29.0	35.0	2.0	35.0
110-111	27.983306938151735	34.0	29.0	35.0	2.0	35.0
112-113	27.886797259798445	34.0	29.0	35.0	2.0	35.0
114-115	27.734252375386294	34.0	29.0	35.0	2.0	35.0
116-117	27.547817230111967	34.0	27.0	35.0	2.0	35.0
118-119	27.409259271262524	34.0	27.0	35.0	2.0	35.0
120-121	27.206164193153676	34.0	27.0	35.0	2.0	35.0
122-123	27.030240127733954	34.0	27.0	35.0	2.0	35.0
124-125	25.94140575767855	33.0	22.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	110617.0
3	15537.0
4	11027.0
5	11322.0
6	14628.0
7	14808.0
8	14108.0
9	14886.0
10	15975.0
11	16579.0
12	17471.0
13	17615.0
14	18344.0
15	16572.0
16	13354.0
17	12053.0
18	11283.0
19	10942.0
20	11128.0
21	12208.0
22	14572.0
23	18703.0
24	25356.0
25	32824.0
26	38069.0
27	43775.0
28	50790.0
29	60436.0
30	71026.0
31	86646.0
32	106196.0
33	133937.0
34	179088.0
35	257334.0
36	437013.0
37	633456.0
38	207185.0
39	159.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.658112845003075	18.79468341466245	13.174343652238843	24.37286008809563
2	16.141437088053173	20.36611813650039	38.81777067756092	24.674674097885514
3	19.451456718054256	24.471156114520504	28.863874811316915	27.21351235610833
4	13.226885320532583	16.745878990269777	35.82249476319861	34.204740925999026
5	14.396410432046814	37.488930256746016	33.5852498979909	14.529409413216273
6	32.706066147739406	35.444875563337625	17.35205927496638	14.496999013956586
7	28.465451989171548	32.32862484644868	20.777208248897367	18.428714915482402
8	26.07460258125018	34.92081155462019	19.908678857704246	19.095907006425396
9	26.492920009795306	16.524286599155875	19.52571988303251	37.45707350801631
10-11	24.969995885407243	26.391015962278995	27.11981207925588	21.51917607305788
12-13	25.504075113975887	24.13400564355974	27.101331085656895	23.260588156807483
14-15	23.421818242713144	25.451375315182585	24.662021090380943	26.46478535172333
16-17	23.078358111528257	28.14968729612484	25.687510050263473	23.084444542083435
18-19	23.01079071402231	27.4075817334286	27.056048043237762	22.52557950931133
20-21	24.346860417333694	26.5049644766834	26.556465812656864	22.59170929332604
22-23	23.9838077616438	26.270109756036074	26.168863251858376	23.577219230461747
24-25	23.37296543593062	26.559774685257796	26.392268688046144	23.67499119076544
26-27	23.348603237685467	26.591150201554093	26.45427203212766	23.605974528632782
28-29	23.269038095999527	26.632392439718487	26.74787033055524	23.350699133726746
30-31	23.673609615596533	26.679779978695066	26.347025484528718	23.29958492117968
32-33	23.289374346516468	26.641349872134647	26.304882938783976	23.764392842564913
34-35	23.398264539746506	26.777478270733962	26.31523506501224	23.509022124507297
36-37	23.73742229924025	26.56886121233532	26.168892038753416	23.524824449671012
38-39	23.565806694741624	26.710888718479364	26.208668763315995	23.514635823463014
40-41	23.483760265179683	26.62164181657445	26.436404949972726	23.458192968273146
42-43	23.56074175382951	26.49494812001213	26.333910217768477	23.610399908389887
44-45	23.586903738818034	26.51521330605891	26.191388030186243	23.706494924936816
46-47	23.567366508289417	26.585194932216123	26.193123013532286	23.654315545962174
48-49	23.42666244620441	26.63732869226528	26.230028877036542	23.705979984493762
50-51	23.2785593557412	26.79455283983397	26.190335145728334	23.736552658696496
52-53	23.414144738855917	26.68850654890368	26.04946131931663	23.847887392923777
54-55	23.516923173877228	26.60136573771894	25.98020265495091	23.90150843345292
56-57	23.583977201033647	26.599334237917038	25.93747929396378	23.879209267085542
58-59	23.4431691632629	26.35757427895198	26.25218877922611	23.94706777855901
60-61	23.621231878499653	26.425511212039694	26.23591000118478	23.717346908275868
62-63	23.311765337128378	26.392406932199812	26.303064436789374	23.992763293882433
64-65	23.42806171725385	26.423122435559947	26.22754618242788	23.92126966475832
66-67	23.414590231527242	26.524552618928016	26.14135063260827	23.919506516936472
68-69	23.71139088077028	26.217038229118316	26.128614419976902	23.9429564701345
70-71	23.408275811654786	26.09266882403345	26.470054967660207	24.02900039665156
72-73	23.581792277142902	26.10038767150246	26.368360128478418	23.94945992287622
74-75	23.468105889078444	26.31382310708193	26.289804057490873	23.928266946348753
76-77	23.403945781825524	25.960153268018633	26.629498998097024	24.00640195205882
78-79	23.526780923682775	26.024679745942947	26.473147056514662	23.97539227385962
80-81	23.383203355828677	26.16494521643532	26.39953129596881	24.052320131767193
82-83	23.506695933034727	26.035646465106588	26.43917232905831	24.018485272800373
84-85	23.576202462360595	26.004677692914324	26.282692536217013	24.136427308508072
86-87	23.467569073655458	25.83163411825817	26.510311872359466	24.190484935726907
88-89	23.486943465827302	25.78793559065754	26.530458284502505	24.194662659012657
90-91	23.521015053128423	25.841290818590938	26.49768319921066	24.140010929069977
92-93	23.45307593292763	25.88405242331526	26.554027131364915	24.108844512392192
94-95	23.254397832475632	25.875110236143918	26.719962923925266	24.150529007455187
96-97	23.39859676010316	25.76303510044285	26.623531419854935	24.21483671959905
98-99	23.370667440644493	25.827098778680273	26.46741298871154	24.33482079196369
100-101	23.12153975430579	26.055671458488217	26.646239262424025	24.176549524781972
102-103	23.08165571461084	26.011288635885677	26.61508013102135	24.291975518482133
104-105	22.977274605583066	26.130233347035585	26.469674716425473	24.422817330955876
106-107	22.94727425909317	26.181279834834463	26.623957609529214	24.247488296543153
108-109	22.904930956524712	26.196779081587174	26.55636157046558	24.341928391422528
110-111	22.897374139556593	26.317611926968276	26.48611730336658	24.29889663010855
112-113	22.77987071013636	26.452674411046555	26.518880414528446	24.24857446428864
114-115	22.782140444331766	26.509231343208334	26.509321373472904	24.199306838986995
116-117	22.784115441304362	26.399146405008928	26.512345690126725	24.304392463559985
118-119	22.88234224404437	26.234959011449426	26.478567660366693	24.40413108413951
120-121	22.83902146542512	26.39925888780955	26.513342630246058	24.248377016519278
122-123	22.799987684558324	26.44908546142305	26.437310170700307	24.31361668331832
124-125	22.78251746689165	26.483271212010905	26.42277770609779	24.31143361499965
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	946.0
1	691.0
2	357.0
3	271.0
4	238.5
5	203.5
6	197.0
7	224.0
8	272.5
9	292.5
10	288.0
11	318.0
12	350.0
13	376.0
14	436.0
15	533.5
16	634.0
17	760.5
18	895.0
19	1091.0
20	1346.5
21	1664.5
22	2058.0
23	2587.5
24	3302.5
25	4263.0
26	5812.0
27	7938.5
28	10752.5
29	14543.5
30	19139.5
31	24084.5
32	29730.5
33	36136.0
34	41990.0
35	47677.5
36	55996.5
37	63880.5
38	69023.0
39	72947.5
40	76774.0
41	79877.0
42	83204.0
43	90477.0
44	96934.5
45	101266.0
46	106324.0
47	111191.0
48	115861.5
49	120110.0
50	125143.5
51	123930.5
52	115138.5
53	110797.5
54	108028.5
55	101475.5
56	92363.5
57	82837.0
58	77507.5
59	71329.5
60	60402.5
61	50027.0
62	40407.0
63	29847.0
64	21434.5
65	15756.5
66	11345.5
67	8221.0
68	6029.5
69	4466.5
70	3515.5
71	2678.0
72	2029.5
73	1827.0
74	1436.0
75	989.5
76	700.0
77	445.0
78	276.0
79	191.0
80	135.5
81	94.0
82	60.5
83	52.5
84	40.5
85	29.5
86	28.0
87	23.0
88	21.0
89	20.5
90	19.0
91	15.0
92	11.5
93	8.5
94	7.0
95	8.5
96	9.5
97	9.5
98	7.5
99	13.0
100	23.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07500840828772692
2	0.009650625742251952
3	0.019481300472232486
4	0.018653075128681012
5	0.011127027441626318
6	0.009650625742251952
7	0.00947057675452337
8	0.008210233840423303
9	0.006553783153320356
10-11	0.011505130315856338
12-13	0.00822823873919616
14-15	0.009308532665567647
16-17	0.01272946343241069
18-19	0.006841861533686086
20-21	0.014223870030557913
22-23	0.004897332466217408
24-25	0.0038350434386187795
26-27	0.0036009797545716237
28-29	0.01080293926371487
30-31	0.0072019595091432475
32-33	0.0027907593097930084
34-35	0.008534322018334747
36-37	0.006589792950866071
38-39	0.001854504573604386
40-41	0.001332362509191501
42-43	0.0012963527116457846
44-45	0.0022146025490615484
46-47	0.0038890581349373538
48-49	0.0027007348159287176
50-51	0.004519229591987388
52-53	0.0052214206441288545
54-55	0.004843317769898834
56-57	0.0019445290674686769
58-59	0.00268272991715586
60-61	0.00507738145394599
62-63	0.002448666233108704
64-65	0.0036009797545716237
66-67	9.722645337343384E-4
68-69	0.001980538865014393
70-71	9.182498374157641E-4
72-73	0.002070563358878684
74-75	0.002142582953970116
76-77	0.0020345535613329677
78-79	2.880783803657299E-4
80-81	1.0802939263714871E-4
82-83	1.800489877285812E-5
84-85	4.3211757054859484E-4
86-87	1.6204408895572307E-4
88-89	9.00244938642906E-5
90-91	8.822400398700479E-4
92-93	0.001854504573604386
94-95	0.001044284128825771
96-97	7.201959509143248E-4
98-99	9.362547361886222E-4
100-101	0.001206328217781494
102-103	0.002520685828200137
104-105	0.0038350434386187795
106-107	0.006175680279090334
108-109	0.011865228291313501
110-111	0.009218508171703357
112-113	0.003006818095067306
114-115	0.006409743963137491
116-117	0.0021965976502886907
118-119	9.722645337343384E-4
120-121	0.004123121818984509
122-123	7.382008496871829E-4
124-125	0.08440696544715887
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2777022.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.55779491849878
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.9876590826625	28.508470573102628
2	13.901042568363275	11.831954375554519
3	6.402685221218121	8.174524937169114
4	3.6305923901052575	6.1804002548304755
5	2.2536449496357385	4.795507979285414
6	1.4741398729577038	3.764168543670948
7	1.0313343720886277	3.0723921689822573
8	0.767137859114145	2.6118156545916795
9	0.5733164605847646	2.19591759176694
>10	2.790464258077967	20.795776218452282
>50	0.13980336975542942	4.02176015419006
>100	0.04664446796251973	3.1980002257051248
>500	7.675637369530801E-4	0.2393678706510746
>1k	7.675637369432196E-4	0.6099434520475342
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	3821	0.13759343642218175	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	3614	0.13013940833021848	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	1.800489877285812E-5	0.0	3.600979754571624E-5	0.0
14-15	0.0	3.600979754571624E-5	0.0	3.600979754571624E-5	0.0
16-17	0.0	3.600979754571624E-5	0.0	1.0802939263714871E-4	0.0
18-19	0.0	3.600979754571624E-5	0.0	1.6204408895572307E-4	0.0
20-21	0.0	3.600979754571624E-5	0.0	2.7007348159287177E-4	0.0
22-23	0.0	3.600979754571624E-5	0.0	7.562057484600409E-4	0.0
24-25	0.0	5.4014696318574355E-5	0.0	0.0020525584601058253	0.0
26-27	0.0	7.201959509143247E-5	0.0	0.004087112021438793	0.0
28-29	0.0	9.00244938642906E-5	0.0	0.009308532665567647	0.0
30-31	0.0	1.0802939263714871E-4	0.0	0.025548951358685672	0.0
32-33	0.0	1.0802939263714871E-4	0.0	0.05813781813755887	0.0
34-35	0.0	1.800489877285812E-4	0.0	0.10430237859116709	0.0
36-37	0.0	2.3406368404715555E-4	0.0	0.1831458303175128	0.0
38-39	0.0	3.2408817791144615E-4	0.0	0.2938759577705902	0.0
40-41	0.0	3.781028742300205E-4	0.0	0.4134825003186867	0.0
42-43	0.0	4.681273680943111E-4	0.0	0.5194233246981839	0.0
44-45	0.0	4.681273680943111E-4	0.0	0.6452775671204621	0.0
46-47	0.0	4.861322668671692E-4	0.0	0.7929897566529902	0.0
48-49	0.0	5.041371656400273E-4	0.0	0.9431866222161726	0.0
50-51	0.0	5.221420644128854E-4	0.0	1.094895899276275	0.0
52-53	0.0	5.401469631857435E-4	0.0	1.2481535976308433	0.0
54-55	0.0	5.401469631857435E-4	0.0	1.4235393165772545	0.0
56-57	0.0	5.761567607314598E-4	0.0	1.6296954075264798	0.0
58-59	0.0	5.761567607314598E-4	0.0	1.8441697617087658	0.0
60-61	0.0	7.021910521414667E-4	0.0	2.0644596981946846	0.0
62-63	0.0	7.201959509143248E-4	0.0	2.289430908361547	0.0
64-65	0.0	7.201959509143248E-4	0.0	2.5358459529668833	0.0
66-67	0.0	7.922155460057573E-4	0.0	2.8260129015902646	3.600979754571624E-5
68-69	0.0	7.922155460057573E-4	0.0	3.146248031164319	3.600979754571624E-5
70-71	0.0	8.102204447786154E-4	0.0	3.4856403730326946	3.600979754571624E-5
72-73	0.0	8.282253435514734E-4	0.0	3.8222059457937316	3.600979754571624E-5
74-75	0.0	8.282253435514734E-4	0.0	4.177694667165042	3.600979754571624E-5
76-77	0.0	9.542596349614804E-4	0.0	4.585685673358007	3.600979754571624E-5
78-79	0.0	9.722645337343384E-4	0.0	5.028948276247002	3.600979754571624E-5
80-81	0.0	9.902694325071965E-4	0.0	5.50134280535048	3.600979754571624E-5
82-83	0.0	0.0010082743312800546	0.0	5.991418145048906	3.600979754571624E-5
84-85	0.0	0.0010082743312800546	0.0	6.492044355428225	3.600979754571624E-5
86-87	0.0	0.0010082743312800546	0.0	7.040527586745801	3.600979754571624E-5
88-89	0.0	0.0010082743312800546	0.0	7.648390974216265	3.600979754571624E-5
90-91	0.0	0.0010082743312800546	0.0	8.273106946938123	3.600979754571624E-5
92-93	0.0	0.0010442841288257707	0.0	8.914459446126102	3.600979754571624E-5
94-95	0.0	0.001080293926371487	0.0	9.578210039387518	3.600979754571624E-5
96-97	0.0	0.001080293926371487	0.0	10.285442463185383	5.4014696318574355E-5
98-99	0.0	0.001080293926371487	0.0	11.045123157108586	7.201959509143247E-5
100-101	0.0	0.0010982988251443454	0.0	11.8515625731449	7.201959509143247E-5
102-103	0.0	0.0011523135214629196	0.0	12.651142122748757	7.201959509143247E-5
104-105	0.0	0.0012603429141000684	0.0	13.461200523438418	7.201959509143247E-5
106-107	0.0	0.0012963527116457846	0.0	14.305198158314914	7.201959509143247E-5
108-109	0.0	0.0012963527116457846	0.0	15.183909238025482	7.201959509143247E-5
110-111	0.0	0.0013323625091915007	0.0	16.103527447747982	7.201959509143247E-5
112-113	0.0	0.001368372306737217	0.0	17.041600678712662	7.201959509143247E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	12070	0.0	26.63677	1
GTACATG	16790	0.0	24.04219	1
AACGCAG	13320	0.0	23.675585	6
CATGGGG	8910	0.0	23.576155	4
TCAACGC	13560	0.0	23.478485	4
TACATGG	16885	0.0	23.437128	2
CAACGCA	13650	0.0	23.10612	5
TATCAAC	13860	0.0	23.013643	2
ATCAACG	13975	0.0	22.90943	3
GTATCGC	130	0.0020525544	22.885254	6
GGTATCA	9475	0.0	21.678783	1
ACATGGG	17655	0.0	21.639694	3
ACGCAGA	14835	0.0	21.057209	7
CGCAGAG	15280	0.0	20.132433	8
GCAGAGT	16170	0.0	18.214792	9
ATGGGGC	5095	0.0	17.987066	5
GTATAGT	900	0.0	17.861433	1
GTATAGG	695	0.0	17.13327	1
TACACCG	360	9.363375E-7	16.530323	5
CAGAGTA	15010	0.0	16.156902	10-11
>>END_MODULE