FastQCFastQC Report
Mon 15 Aug 2016
SRR2921131_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921131_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2777022
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40280.145047464514145No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT38480.13856570095591608No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37120.13366836848969868No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA35280.1270425657412869No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT34330.12362163497444385No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA131100.030.9865321
GGTATCA96700.030.5973471
TATCAAC159150.025.3258912
TCAACGC159050.025.1187024
ATCAACG160350.024.9513453
CAACGCA161050.024.6529675
AACGCAG162950.024.2771426
CATGGGG84800.023.4155544
GTACATG162300.023.0450481
TACATGG164500.022.2912242
GAGTACT73350.022.1841212-13
ACGCAGA180200.021.6890667
ACATGGG168350.021.3217953
GTACTTT77400.020.98470114-15
CGCAGAG186450.020.9308748
AGTACTT80150.020.48757712-13
GCAGAGT195350.019.2474449
AGAGTAC178600.018.05522310-11
TACTTTT90900.017.76998314-15
TAATACG2351.6664604E-417.761774
Produced by FastQC (version 0.11.5)