##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921131_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2777022
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.724007227886563	31.0	31.0	34.0	27.0	34.0
2	30.8389973143893	31.0	31.0	34.0	27.0	34.0
3	30.941763515017165	31.0	31.0	34.0	27.0	34.0
4	34.4556150437411	37.0	35.0	37.0	31.0	37.0
5	34.09707665261564	37.0	35.0	37.0	30.0	37.0
6	33.94308363419519	37.0	35.0	37.0	29.0	37.0
7	33.77068960922888	37.0	35.0	37.0	28.0	37.0
8	33.77084085037857	37.0	35.0	37.0	28.0	37.0
9	35.19692497934838	39.0	35.0	39.0	28.0	39.0
10-11	35.169820404735724	39.0	35.0	39.0	28.0	39.0
12-13	35.04586297839916	39.0	35.0	39.0	27.0	39.0
14-15	36.15423194342716	40.0	36.0	41.0	27.0	41.0
16-17	36.098932597581154	40.0	36.0	41.0	27.0	41.0
18-19	36.15507169190593	40.0	36.0	41.0	27.0	41.0
20-21	36.15576091943096	40.0	36.0	41.0	27.0	41.0
22-23	36.11040063780553	40.0	36.0	41.0	27.0	41.0
24-25	36.00573492035713	40.0	36.0	41.0	27.0	41.0
26-27	35.89186851958681	40.0	35.5	41.0	27.0	41.0
28-29	35.793538185869615	40.0	35.0	41.0	27.0	41.0
30-31	35.695414548390325	39.0	35.0	41.0	27.0	41.0
32-33	35.53645253800654	39.0	35.0	41.0	26.0	41.0
34-35	35.40767411997456	39.0	35.0	41.0	25.5	41.0
36-37	35.26079771784307	39.0	35.0	41.0	25.0	41.0
38-39	35.07329524216949	39.0	35.0	41.0	24.5	41.0
40-41	34.921934359900646	39.0	34.0	41.0	24.0	41.0
42-43	34.77159867656792	39.0	34.0	41.0	24.0	41.0
44-45	34.582467657800336	39.0	34.0	41.0	23.0	41.0
46-47	34.333861597063326	39.0	33.5	41.0	21.5	41.0
48-49	34.10650743854388	38.0	33.0	41.0	19.5	41.0
50-51	33.883550436402736	38.0	33.0	40.5	18.5	41.0
52-53	33.70377548323348	38.0	33.0	40.0	17.5	41.0
54-55	33.48548265011945	38.0	33.0	40.0	14.5	41.0
56-57	33.21046214253974	38.0	32.5	40.0	13.0	41.0
58-59	32.97654573856455	37.0	32.0	40.0	12.5	41.0
60-61	32.703215710930635	37.0	32.0	40.0	8.5	41.0
62-63	32.409281417288014	36.0	31.5	40.0	8.0	41.0
64-65	32.11463214911514	36.0	31.0	39.0	7.0	41.0
66-67	31.766072252938578	35.5	31.0	39.0	4.0	41.0
68-69	31.395214549974757	35.0	31.0	38.5	2.0	40.0
70-71	31.025098828889362	35.0	30.5	37.5	2.0	40.0
72-73	30.824172980984667	35.0	30.5	37.0	2.0	39.5
74-75	30.621645957432097	35.0	30.0	37.0	2.0	39.0
76-77	30.385069329663214	35.0	30.0	36.0	2.0	39.0
78-79	30.0620018494632	35.0	30.0	36.0	2.0	38.5
80-81	29.77257976350205	35.0	30.0	35.5	2.0	37.0
82-83	29.553886321390323	34.5	30.0	35.0	2.0	37.0
84-85	29.39345133023793	34.0	30.0	35.0	2.0	36.5
86-87	29.19216898533753	34.0	29.5	35.0	2.0	36.0
88-89	29.04426432343712	34.0	29.0	35.0	2.0	36.0
90-91	28.888176254995457	34.0	29.0	35.0	2.0	35.5
92-93	28.76413582607556	34.0	29.0	35.0	2.0	35.0
94-95	28.66278012921756	34.0	29.0	35.0	2.0	35.0
96-97	28.60218410225054	34.0	29.0	35.0	2.0	35.0
98-99	28.558855133304668	34.0	29.0	35.0	2.0	35.0
100-101	28.40599480306602	34.0	29.0	35.0	2.0	35.0
102-103	27.022056541143712	32.0	26.0	34.0	2.0	35.0
104-105	28.116359719152385	34.0	29.0	35.0	2.0	35.0
106-107	28.390315056920688	34.0	29.0	35.0	2.0	35.0
108-109	28.588951041799454	34.0	29.0	35.0	2.0	35.0
110-111	28.558571736197983	34.0	29.0	35.0	2.0	35.0
112-113	28.474697175607538	34.0	29.0	35.0	2.0	35.0
114-115	28.34971887151056	34.0	29.0	35.0	2.0	35.0
116-117	28.242667144876776	34.0	29.0	35.0	2.0	35.0
118-119	28.095191359665137	34.0	29.0	35.0	2.0	35.0
120-121	27.91592054366152	34.0	29.0	35.0	2.0	35.0
122-123	27.726638643842215	34.0	29.0	35.0	2.0	35.0
124-125	26.930582832977198	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	62879.0
3	21812.0
4	10846.0
5	9120.0
6	10431.0
7	12589.0
8	14274.0
9	15586.0
10	17102.0
11	18662.0
12	20162.0
13	21820.0
14	24906.0
15	20928.0
16	14374.0
17	12867.0
18	12322.0
19	12193.0
20	12209.0
21	12547.0
22	13285.0
23	15651.0
24	21079.0
25	28013.0
26	34844.0
27	41321.0
28	49121.0
29	58470.0
30	69925.0
31	84625.0
32	103300.0
33	131296.0
34	172936.0
35	247837.0
36	432964.0
37	674023.0
38	240360.0
39	343.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.806288383357845	19.402841449701583	13.87142115733933	24.919449009601248
2	16.01818789403863	20.40603353532502	38.15831548441274	25.417463086223613
3	18.737446011999975	24.291737216268167	29.845776350501225	27.125040421230633
4	13.313647891541272	16.864531211105618	35.52593319456119	34.295887702791916
5	14.006473924111942	37.13093154737021	34.08046780624645	14.782126722271393
6	32.503045828052876	35.71118599484505	17.015725944260716	14.770042232841355
7	28.08433168686783	32.06826199149697	20.9669548431476	18.880451478487608
8	26.179976511931848	34.56106087414364	19.98531365502359	19.27364895890092
9	26.296681674819045	15.58679642757711	19.70238693372318	38.41413496388067
10-11	25.09958107427445	25.760393501689983	27.425075359989254	21.714950064046313
12-13	25.857218775892864	23.365848906045798	27.563921670887563	23.21301064717377
14-15	23.475827520092555	24.64159736222286	25.295854844455363	26.58672027322922
16-17	22.925521923215392	27.56044897310091	26.264166384361044	23.249862719322653
18-19	22.85210327812934	26.47037847732367	28.128947462260147	22.54857078228684
20-21	23.98378164108137	25.561925289866334	27.817929056116075	22.636364012936216
22-23	23.60187133963669	25.458658673946662	27.467939008563164	23.471530977853487
24-25	23.153470744158756	25.777532923019514	27.68258538769397	23.38641094512776
26-27	23.069249531250815	25.831785544029106	27.751302421250944	23.34766250346913
28-29	22.963611719545533	25.71213841976104	28.095280289858515	23.228969570834916
30-31	23.360066132611014	25.741812667492724	27.70896968561796	23.189151514278304
32-33	22.963406657369095	25.726979143854955	27.693840694551696	23.61577350422425
34-35	23.05652362701693	25.896635481668557	27.65805628860042	23.388784602714097
36-37	23.507429690124155	25.68896068986571	27.50767462991129	23.29593499009885
38-39	23.306697885944573	25.806227345385775	27.505612715852042	23.381462052817614
40-41	23.230426148633146	25.732397374676307	27.624360139486082	23.412816337204465
42-43	23.50931969546038	25.54079051851809	27.46214641970715	23.48774336631438
44-45	23.42073584088743	25.699679921965217	27.241734919483406	23.637849317663942
46-47	23.527942323312914	25.70477619305769	27.03803628386641	23.729245199762982
48-49	23.41346510574138	25.91902826133468	26.898334547178226	23.769172085745716
50-51	23.237675879401507	26.11779169567897	26.851261166209973	23.793271258709545
52-53	23.429171213444402	26.019929519617136	26.70717358608345	23.843725680855016
54-55	23.338700497813512	26.097837284863157	26.528219037944773	24.03524317937856
56-57	23.443584041310878	26.162894988794104	26.37833196096272	24.015189008932296
58-59	23.170328689702146	25.99517381244864	26.83929739152109	23.995200106328127
60-61	23.418169597728173	26.195187462888597	26.602298516048243	23.784344423334982
62-63	23.00641225256599	26.24666829601002	26.688121888811185	24.058797562612803
64-65	23.034351413144538	26.246045180135898	26.639266754873752	24.08033665184581
66-67	23.129373000422003	26.334479384320446	26.46614106459365	24.0700065506639
68-69	23.33105083112017	25.982757682913256	26.471502521618635	24.21468896434794
70-71	23.132488584470774	25.768178524449525	26.740193884327564	24.35913900675214
72-73	23.265779445490367	25.767510441816547	26.56951472301537	24.397195389677716
74-75	23.1295777118505	25.94886019713708	26.494083924544665	24.42747816646775
76-77	23.009977552532746	25.676877127804694	26.78756304064585	24.525582279016707
78-79	23.04510659387641	25.763647542356427	26.619222375619465	24.572023488147693
80-81	22.87306598566069	25.899376324836965	26.5448071793782	24.68275051012414
82-83	22.973378671420672	25.723847681459656	26.551416052830962	24.751357594288713
84-85	23.007635998954807	25.683810252368115	26.390810509344526	24.91774323933255
86-87	22.898415168654964	25.534086200404353	26.571906092525538	24.99559253841515
88-89	22.850184528956838	25.53623607028776	26.551604539336704	25.0619748614187
90-91	22.835242501138588	25.558627200027082	26.553090856506177	25.05303944232815
92-93	22.800853055714438	25.54427144489283	26.56819763345834	25.08667786593439
94-95	22.659122173854087	25.482832473109646	26.704331504356283	25.15371384867999
96-97	22.732519839404645	25.2782991328038	26.684553015953284	25.304628011838272
98-99	22.717853625471793	25.305432404548938	26.477531451753627	25.499182518225638
100-101	22.511315048812328	25.47965488223219	26.587307804443828	25.421722264511654
102-103	22.5136598835754	25.319432924398207	26.59612016447685	25.570787027549546
104-105	22.40622840136906	25.369790171607708	26.45577698885686	25.76820443816637
106-107	22.276647994310956	25.411115066838896	26.586632509347435	25.72560442950272
108-109	22.18346571970345	25.387786119329608	26.577732798625654	25.851015362341283
110-111	22.1341712659926	25.518092682795068	26.436889445109706	25.910846606102623
112-113	21.96844330913089	25.60001613416131	26.435751284700604	25.99578927200719
114-115	21.90618390484206	25.604667720407825	26.453368712530693	26.035779662219415
116-117	21.887561771232704	25.478198113064852	26.499507702812398	26.13473241289005
118-119	21.83859119942583	25.405733110493873	26.401866550565433	26.353809139514862
120-121	21.742571832923407	25.58609687676679	26.36134148142013	26.30998980888967
122-123	21.58910130100797	25.697288811958046	26.24466181229278	26.4689480747412
124-125	21.38311811024757	25.702790552967294	26.342884144879385	26.571207191905756
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	89.0
1	74.5
2	130.5
3	216.5
4	193.0
5	153.0
6	203.5
7	465.5
8	779.5
9	869.5
10	719.0
11	585.5
12	574.0
13	584.5
14	664.0
15	762.5
16	882.5
17	1017.0
18	1181.0
19	1383.5
20	1593.0
21	1916.0
22	2291.5
23	2740.5
24	3404.0
25	4341.5
26	5854.0
27	8125.0
28	10785.0
29	14349.0
30	19063.5
31	24102.5
32	29543.5
33	35600.5
34	41715.5
35	47541.5
36	55571.0
37	63149.5
38	68213.5
39	72396.0
40	76304.0
41	79376.5
42	82799.5
43	89694.5
44	96245.5
45	101375.5
46	106358.0
47	110850.5
48	115861.0
49	119970.0
50	125835.0
51	125362.5
52	116507.5
53	112247.0
54	109017.0
55	102443.5
56	93404.0
57	83732.0
58	77994.0
59	71634.0
60	60883.5
61	50390.5
62	40254.0
63	29307.0
64	20737.5
65	15081.5
66	10655.0
67	7555.5
68	5431.5
69	3907.5
70	2977.0
71	2293.0
72	1749.0
73	1560.5
74	1205.5
75	863.5
76	614.5
77	334.5
78	167.5
79	88.0
80	51.0
81	27.5
82	18.0
83	16.0
84	15.0
85	12.0
86	8.0
87	3.0
88	3.0
89	2.5
90	1.5
91	1.0
92	1.5
93	2.0
94	3.0
95	2.5
96	2.0
97	3.0
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08577533775389608
2	3.600979754571624E-4
3	5.761567607314598E-4
4	0.18336188910278708
5	0.07914953500548429
6	0.009830674729980534
7	0.01321559569927786
8	0.010550870680894858
9	0.019121202496775323
10-11	0.01856305063481672
12-13	0.020075462131736804
14-15	0.026827299171558596
16-17	0.027925597996702942
18-19	0.025674985650095676
20-21	0.02828569597216011
22-23	0.029852122165398766
24-25	0.02783557350283865
26-27	0.027025353058060038
28-29	0.02776355390774722
30-31	0.028159661680750098
32-33	0.02412656435562988
34-35	0.026665255082602875
36-37	0.02987012706417162
38-39	0.03455140074511473
40-41	0.029924141760490193
42-43	0.029942146659263053
44-45	0.029672073177670177
46-47	0.030860396496678818
48-49	0.02592705423291569
50-51	0.03244482758869033
52-53	0.028393725364797253
54-55	0.03176064143532172
56-57	0.028951877226755855
58-59	0.025584961156231385
60-61	0.02709737265315147
62-63	0.030698352407723093
64-65	0.0352535917972562
66-67	0.03483947912548046
68-69	0.029852122165398766
70-71	0.03919666462851213
72-73	0.041915404343213704
74-75	0.026305157107145715
76-77	0.019427285775913912
78-79	0.02315429982189554
80-81	0.02653922079119287
82-83	0.025044814193045644
84-85	0.01809492326672241
86-87	0.017302707720716654
88-89	0.017752830190038107
90-91	0.01982339354891679
92-93	0.023406368404715553
94-95	0.01856305063481672
96-97	0.021713907920066892
98-99	0.018274972254450992
100-101	0.018743099622545303
102-103	0.016600516668575187
104-105	0.01854504573604386
106-107	0.01647448237716518
108-109	0.015376183552020833
110-111	0.012495399748363535
112-113	0.010982988251443454
114-115	0.008966439588883343
116-117	0.008786390601154763
118-119	0.004861322668671692
120-121	0.0033129013742058937
122-123	0.007688091776010417
124-125	0.05648136745045593
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2777022.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.226186986214266
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.67358902951412	27.07471661386539
2	14.494177369428776	11.950793328868519
3	6.6288500635357055	8.19846636668704
4	3.6821090628480935	6.071972669144388
5	2.40368986977372	4.95474840140802
6	1.5322132035385012	3.790038481909478
7	1.0820107885913393	3.1225025364097396
8	0.796512688826795	2.62697448371726
9	0.5970029443256517	2.2150939512680807
>10	2.9084659983891816	20.978174976850564
>50	0.14812866733369626	4.168929387263261
>100	0.05114464551419504	3.4567329236441346
>500	9.650980076162524E-4	0.2736643585770413
>1k	0.0011405703726167903	1.1171915203872207
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4028	0.145047464514145	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	3848	0.13856570095591608	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3712	0.13366836848969868	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	3528	0.1270425657412869	No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT	3433	0.12362163497444385	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	3.600979754571624E-5	0.0
7	0.0	0.0	0.0	3.600979754571624E-5	0.0
8	0.0	0.0	0.0	3.600979754571624E-5	0.0
9	0.0	0.0	0.0	3.600979754571624E-5	0.0
10-11	0.0	0.0	0.0	3.600979754571624E-5	0.0
12-13	0.0	1.800489877285812E-5	0.0	7.201959509143247E-5	0.0
14-15	0.0	5.4014696318574355E-5	0.0	7.201959509143247E-5	0.0
16-17	0.0	7.201959509143247E-5	0.0	1.6204408895572307E-4	0.0
18-19	0.0	7.201959509143247E-5	0.0	2.3406368404715552E-4	0.0
20-21	0.0	7.201959509143247E-5	0.0	3.2408817791144615E-4	0.0
22-23	0.0	7.201959509143247E-5	0.0	7.922155460057573E-4	0.0
24-25	0.0	9.00244938642906E-5	0.0	0.002070563358878684	0.0
26-27	0.0	1.0802939263714871E-4	0.0	0.004123121818984509	0.0
28-29	0.0	1.0802939263714871E-4	0.0	0.009254517969249074	0.0
30-31	0.0	1.0802939263714871E-4	0.0	0.025242868079547086	0.0
32-33	0.0	1.0802939263714871E-4	0.0	0.05790375445351171	0.0
34-35	0.0	1.0802939263714871E-4	0.0	0.10397829041325564	0.0
36-37	0.0	1.2603429141000682E-4	0.0	0.18195750699850416	0.0
38-39	0.0	1.800489877285812E-4	0.0	0.2910851984607972	0.0
40-41	0.0	2.1605878527429742E-4	0.0	0.4096114470825222	0.0
42-43	0.0	2.880783803657299E-4	0.0	0.5141118795601908	0.0
44-45	0.0	2.880783803657299E-4	0.0	0.6381116174088646	0.0
46-47	0.0	3.2408817791144615E-4	0.0	0.7839693023677883	0.0
48-49	0.0	3.600979754571624E-4	0.0	0.9325097172438677	0.0
50-51	0.0	4.321175705485949E-4	0.0	1.0823824946291387	0.0
52-53	0.0	5.041371656400273E-4	0.0	1.233731673713784	0.0
54-55	0.0	5.041371656400273E-4	0.0	1.4066327166295407	0.0
56-57	0.0	5.041371656400273E-4	0.0	1.6108442785112973	0.0
58-59	0.0	5.041371656400273E-4	0.0	1.8234101134236602	0.0
60-61	0.0	5.221420644128854E-4	0.0	2.0419355698298394	0.0
62-63	0.0	5.401469631857435E-4	0.0	2.26633062323597	0.0
64-65	0.0	5.401469631857435E-4	0.0	2.510224982013106	0.0
66-67	0.0	5.761567607314598E-4	0.0	2.797421122338966	0.0
68-69	0.0	5.761567607314598E-4	0.0	3.115333619971322	0.0
70-71	0.0	6.481763558228923E-4	0.0	3.4549240157261987	0.0
72-73	0.0	7.201959509143248E-4	0.0	3.790679368042457	0.0
74-75	0.0	7.382008496871829E-4	0.0	4.144493633827892	0.0
76-77	0.0	9.362547361886222E-4	0.0	4.55036006196566	0.0
78-79	0.0	9.362547361886222E-4	0.0	4.9925063611307365	0.0
80-81	0.0	9.722645337343384E-4	0.0	5.46380259140907	0.0
82-83	0.0	0.0010442841288257707	0.0	5.952041431432664	0.0
84-85	0.0	0.001080293926371487	0.0	6.450975181327335	0.0
86-87	0.0	0.001080293926371487	0.0	6.997477873779898	0.0
88-89	0.0	0.001080293926371487	0.0	7.603792839955895	0.0
90-91	0.0	0.001080293926371487	0.0	8.222261112803572	0.0
92-93	0.0	0.0010982988251443454	0.0	8.85806810316951	0.0
94-95	0.0	0.0013323625091915007	0.0	9.516939368863481	0.0
96-97	0.0	0.0013323625091915007	0.0	10.218158156471212	0.0
98-99	0.0	0.0013323625091915007	0.0	10.973859767765614	0.0
100-101	0.0	0.0013323625091915007	0.0	11.777292365706861	0.0
102-103	0.0	0.0013323625091915007	0.0	12.573685048227922	0.0
104-105	0.0	0.0014403919018286496	0.0	13.382555125598573	0.0
106-107	0.0	0.001512411496920082	0.0	14.227380985818622	0.0
108-109	0.0	0.001512411496920082	0.0	15.109332947308303	0.0
110-111	0.0	0.0015844310920115146	0.0	16.029707362779263	0.0
112-113	0.0	0.001674455585875805	0.0	16.96761854965499	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	13110	0.0	30.986532	1
GGTATCA	9670	0.0	30.597347	1
TATCAAC	15915	0.0	25.325891	2
TCAACGC	15905	0.0	25.118702	4
ATCAACG	16035	0.0	24.951345	3
CAACGCA	16105	0.0	24.652967	5
AACGCAG	16295	0.0	24.277142	6
CATGGGG	8480	0.0	23.415554	4
GTACATG	16230	0.0	23.045048	1
TACATGG	16450	0.0	22.291224	2
GAGTACT	7335	0.0	22.18412	12-13
ACGCAGA	18020	0.0	21.689066	7
ACATGGG	16835	0.0	21.321795	3
GTACTTT	7740	0.0	20.984701	14-15
CGCAGAG	18645	0.0	20.930874	8
AGTACTT	8015	0.0	20.487577	12-13
GCAGAGT	19535	0.0	19.247444	9
AGAGTAC	17860	0.0	18.055223	10-11
TACTTTT	9090	0.0	17.769983	14-15
TAATACG	235	1.6664604E-4	17.76177	4
>>END_MODULE