FastQCFastQC Report
Mon 15 Aug 2016
SRR2921130_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921130_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2714473
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA68200.035.5334241
TCAACGC75400.031.8840084
CAACGCA77200.031.1405985
ATCAACG79200.030.205623
AACGCAG82300.029.4272356
TATCAAC82600.029.1063822
GGTATCA47500.027.0761661
GTACATG125750.026.2791921
TACATGG123350.026.1002712
ACGCAGA92400.025.8886247
ACATGGG122400.025.622183
CATGGGG76750.025.5082344
CGCAGAG95100.024.9663648
GTCCGTA1903.289594E-521.9367081
GCAGAGT105150.021.5614819
TATAGCG1703.9312587E-421.0033822
CCTATAC6750.019.3957163
GTATAAG10250.018.5888751
AGAGTAC94100.018.53023310-11
ATGGGGA39250.018.0413995
Produced by FastQC (version 0.11.5)