##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921130_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2714473
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.303773513311793	31.0	30.0	34.0	24.0	34.0
2	29.40383492486387	31.0	30.0	34.0	23.0	34.0
3	29.556559228992146	31.0	30.0	34.0	24.0	34.0
4	32.955728423159854	35.0	35.0	37.0	26.0	37.0
5	33.07723340773697	37.0	35.0	37.0	26.0	37.0
6	33.082169172432366	37.0	35.0	37.0	26.0	37.0
7	32.944517407246266	37.0	35.0	37.0	26.0	37.0
8	32.94897609959649	37.0	35.0	37.0	26.0	37.0
9	34.29308561919754	39.0	35.0	39.0	25.0	39.0
10-11	34.233837470477695	38.5	34.5	39.0	25.0	39.0
12-13	34.22163123376066	39.0	35.0	39.0	25.0	39.0
14-15	35.252465948270626	39.0	35.0	41.0	25.0	41.0
16-17	35.1660001775667	39.0	35.0	41.0	25.0	41.0
18-19	35.22008526148538	39.5	35.0	41.0	25.0	41.0
20-21	35.24390074979563	40.0	35.0	41.0	25.0	41.0
22-23	35.23201022813636	39.0	35.0	41.0	25.0	41.0
24-25	35.15080625226333	39.0	34.5	41.0	24.5	41.0
26-27	35.094841061229936	39.0	34.5	41.0	24.0	41.0
28-29	34.960172563882566	39.0	34.0	41.0	24.0	41.0
30-31	34.86098296059677	39.0	34.0	41.0	24.0	41.0
32-33	34.76542629084909	39.0	34.0	41.0	23.5	41.0
34-35	34.66669239296172	39.0	34.0	41.0	23.0	41.0
36-37	34.54123010249135	39.0	34.0	41.0	22.0	41.0
38-39	34.387430819905006	39.0	33.5	41.0	21.0	41.0
40-41	34.271068638369215	39.0	33.0	41.0	20.0	41.0
42-43	34.186103158882034	39.0	33.0	41.0	19.5	41.0
44-45	34.02179078590946	38.0	33.0	41.0	18.5	41.0
46-47	33.789092026334394	38.0	33.0	40.0	17.0	41.0
48-49	33.64488263467716	38.0	33.0	40.0	15.5	41.0
50-51	33.47730848676704	38.0	33.0	40.0	14.5	41.0
52-53	33.26541487058445	38.0	32.5	40.0	12.0	41.0
54-55	33.01178866026665	38.0	32.0	40.0	9.0	41.0
56-57	32.733765817527015	37.0	32.0	40.0	8.0	41.0
58-59	32.50571344787736	37.0	31.0	40.0	7.5	41.0
60-61	32.202673962864985	36.5	31.0	40.0	6.5	41.0
62-63	31.92122596172443	36.0	31.0	39.5	2.0	41.0
64-65	31.591498423450886	35.5	31.0	39.0	2.0	41.0
66-67	31.30644309226874	35.0	30.0	39.0	2.0	40.5
68-69	30.943163369095956	35.0	30.0	38.5	2.0	40.0
70-71	30.624536143848182	35.0	30.0	37.5	2.0	40.0
72-73	30.270033262441736	35.0	29.5	37.0	2.0	39.0
74-75	30.19843999185109	35.0	30.0	37.0	2.0	39.0
76-77	29.954779620206203	35.0	30.0	36.0	2.0	39.0
78-79	29.69624177510699	34.5	29.5	36.0	2.0	38.5
80-81	29.355397346004178	34.0	29.0	35.0	2.0	37.0
82-83	29.106652377828034	34.0	29.0	35.0	2.0	37.0
84-85	28.93073333203167	34.0	29.0	35.0	2.0	36.0
86-87	28.725938147109957	34.0	29.0	35.0	2.0	36.0
88-89	28.571419756247344	34.0	29.0	35.0	2.0	36.0
90-91	28.415918117439368	34.0	29.0	35.0	2.0	35.0
92-93	28.600208401409773	34.0	29.0	35.0	2.0	35.0
94-95	28.58362875593163	34.0	29.0	35.0	2.0	35.0
96-97	28.523514140682188	34.0	29.0	35.0	2.0	35.0
98-99	28.41850296540065	34.0	29.0	35.0	2.0	35.0
100-101	28.32027284854187	34.0	29.0	35.0	2.0	35.0
102-103	27.00488511029581	32.5	26.0	34.0	2.0	35.0
104-105	28.023920849461387	34.0	28.5	35.0	2.0	35.0
106-107	28.19243090647798	34.0	29.0	35.0	2.0	35.0
108-109	28.14196365187644	34.0	29.0	35.0	2.0	35.0
110-111	28.116871304301057	34.0	29.0	35.0	2.0	35.0
112-113	28.037026524117202	34.0	29.0	35.0	2.0	35.0
114-115	27.90721219183245	34.0	29.0	35.0	2.0	35.0
116-117	27.75854042386865	34.0	29.0	35.0	2.0	35.0
118-119	27.65232127930541	34.0	29.0	35.0	2.0	35.0
120-121	27.479803261995976	34.0	27.5	35.0	2.0	35.0
122-123	27.335632551880238	34.0	28.0	35.0	2.0	35.0
124-125	26.291571144748907	33.0	24.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	116081.0
3	16663.0
4	10862.0
5	9531.0
6	10539.0
7	12061.0
8	13126.0
9	14114.0
10	15500.0
11	16517.0
12	17312.0
13	17545.0
14	18005.0
15	16091.0
16	13314.0
17	11668.0
18	10766.0
19	10426.0
20	10317.0
21	11083.0
22	13460.0
23	17684.0
24	24915.0
25	32405.0
26	37278.0
27	42131.0
28	50055.0
29	58611.0
30	68926.0
31	84018.0
32	103177.0
33	131126.0
34	175660.0
35	253603.0
36	432975.0
37	619367.0
38	197320.0
39	241.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.06712303567354	18.3507454735747	13.03394454928553	24.548186941466234
2	15.998991957396935	19.426115745839155	39.48354764677686	25.09134464998705
3	19.72001781945969	23.637535387435072	28.511135504168006	28.131311288937233
4	13.023566358660988	15.747789813298962	35.99333213211487	35.23531169592518
5	14.393178503871965	37.17885610321702	33.91916955955619	14.508795833354826
6	33.81455483817724	35.31259993721916	16.81984847122766	14.052996753375943
7	29.442938572074382	31.611689532305963	20.33587196764853	18.609499927971118
8	26.742219233851284	35.32743232414409	19.162265471727657	18.768082970276968
9	26.3701741523566	16.434905629096374	18.953538494412904	38.24138172413412
10-11	25.66401987790372	26.026856465670257	27.035544390282183	21.273579266143845
12-13	25.885538103767185	23.34186058378415	27.245845475316383	23.526755837132278
14-15	23.256295910946122	24.843638434881154	24.488122194868364	27.41194345930436
16-17	23.60236364078496	27.778969079457855	25.269737719583308	23.348929560173875
18-19	23.625666746982095	26.906809790229367	26.64344519282702	22.824078269961515
20-21	24.569393279150983	26.102365548293704	26.34331040436328	22.984930768192037
22-23	24.109481140929596	25.8119635762852	26.219762110767274	23.858793172017926
24-25	23.775005443274082	26.041304779507797	26.39873723409468	23.784952543123442
26-27	23.666273819071762	26.193748897073316	26.276880556770283	23.86309672708464
28-29	23.63598582337132	26.339927761017073	26.387105818670094	23.636980596941516
30-31	23.87733786245733	26.274341124826613	26.200693737024665	23.647627275691388
32-33	23.649212336406524	26.198515798309636	26.15968568979717	23.99258617548667
34-35	23.760899431776107	26.270416804365997	26.270803651988935	23.69788011186896
36-37	23.918226986464987	26.052557313246094	26.238959375808218	23.790256324480694
38-39	23.825562448354663	26.25559648500402	26.116211635089098	23.802629431552223
40-41	23.870397553528086	26.22454838282452	26.315837947392083	23.589216116255308
42-43	23.865597051614248	26.046253832980682	26.18749872210952	23.900650393295546
44-45	23.825970412745008	26.179795108480214	26.207443822087434	23.786790656687344
46-47	23.895338127588087	26.20247498489515	26.122492963350474	23.77969392416629
48-49	23.851789604914238	26.291962343031493	26.03435457880532	23.82189347324895
50-51	23.714579971937894	26.550724274606335	25.991711049827465	23.74298470362831
52-53	23.940979293090333	26.310951612282267	25.936605846992755	23.81146324763464
54-55	23.843475893402037	26.28146288061195	26.05122185792108	23.82383936806493
56-57	23.756086023660338	26.407006147915425	25.953446339838848	23.88346148858539
58-59	23.801938148636832	26.143399568485716	26.087199189358806	23.967463093518646
60-61	23.792933723214833	26.30022005438545	26.1535908137886	23.753255408611114
62-63	23.669290627881036	26.29441231650001	26.244217200254568	23.792079855364385
64-65	23.655571120818678	26.185193474378565	26.193482667757156	23.965752737045605
66-67	23.644715603350157	26.35809344802122	26.02598068588377	23.97121026274485
68-69	23.839159158163476	26.146169212788354	26.11958897334516	23.89508265570301
70-71	23.60310583226789	26.1588986168978	26.169103294360923	24.068892256473383
72-73	23.777682443435964	26.160766733709757	26.0837706422745	23.97778018057978
74-75	23.647777796402647	26.325964209921743	26.120984235441092	23.905273758234518
76-77	23.71302346280463	26.04029436816061	26.190234097547034	24.056448071487726
78-79	23.745448229126506	26.188728499220197	26.169442934529837	23.89638033712346
80-81	23.75698525199588	26.250548449807308	26.107205497649815	23.885260800546995
82-83	23.758667660107076	26.172175256886927	26.206178180106814	23.862978902899183
84-85	23.785275393328256	26.20022818509591	26.018184812244634	23.9963116093312
86-87	23.6313849285781	26.1688798378025	26.182307880898232	24.017427352721167
88-89	23.693815961262448	26.079804838657388	26.07243692327642	24.15394227680374
90-91	23.615676428899697	26.158302349548666	26.150473897638847	24.075547323912787
92-93	23.603093250654098	26.195077707036408	26.352901129004806	23.848927913304692
94-95	23.596822546107617	26.03194952913817	26.35297368239276	24.018254242361447
96-97	23.65571631059359	25.855906220326602	26.43350074002168	24.054876729058122
98-99	23.52891767487088	26.0128936426447	26.274513126747944	24.18367555573648
100-101	23.50330307053673	26.028221617391996	26.413512306756004	24.05496300531527
102-103	23.40577870795638	25.87500501950145	26.51606443087327	24.203151841668898
104-105	23.445256528597213	26.041866799246154	26.31753167843531	24.195344993721324
106-107	23.320215768377807	26.052449259928757	26.397786979660864	24.229547992032565
108-109	23.274059415644047	25.994855480563807	26.402459996230863	24.32862510756128
110-111	23.29122481893953	26.17001032525949	26.357615040062193	24.181149815738788
112-113	23.294175162173552	26.17026896789538	26.34485789735836	24.190697972572703
114-115	23.25140617349493	26.32678783073676	26.31976939749422	24.102036598274093
116-117	23.1479288109705	26.250116507951827	26.352293521200025	24.249661159877643
118-119	23.243631058679636	26.16914891363512	26.279282025682992	24.307938002002253
120-121	23.084476928509577	26.322518799575516	26.367870248436414	24.225134023478493
122-123	23.168920713617595	26.251919817303758	26.320589319902993	24.258570149175654
124-125	23.154411473268645	26.37408319944842	26.241129260466757	24.230376066816177
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	309.0
1	244.0
2	171.5
3	144.5
4	118.0
5	109.5
6	123.5
7	145.0
8	169.0
9	220.5
10	245.0
11	281.5
12	342.5
13	380.0
14	402.0
15	459.0
16	536.5
17	608.5
18	732.0
19	895.5
20	1125.0
21	1430.5
22	1911.0
23	2581.5
24	3381.5
25	4595.0
26	6428.0
27	9042.5
28	11943.0
29	15165.0
30	18969.5
31	23824.5
32	29503.5
33	34177.5
34	38542.0
35	44279.0
36	50829.0
37	56518.0
38	61066.5
39	66004.5
40	72242.5
41	77444.5
42	81339.5
43	86492.5
44	92594.0
45	98138.5
46	102810.5
47	106663.0
48	110168.5
49	113478.0
50	114956.0
51	113540.5
52	110875.0
53	109645.5
54	108744.0
55	103492.0
56	94816.5
57	87259.0
58	80834.0
59	72892.0
60	62967.0
61	53260.5
62	43873.0
63	33189.5
64	23889.5
65	18007.0
66	13491.0
67	9852.0
68	7156.5
69	5320.0
70	4149.5
71	3165.5
72	2433.5
73	2006.5
74	1602.0
75	1223.5
76	871.5
77	530.5
78	336.5
79	227.5
80	158.0
81	114.0
82	75.5
83	65.5
84	54.0
85	33.0
86	29.5
87	31.0
88	23.5
89	14.5
90	11.0
91	11.0
92	14.0
93	8.5
94	5.5
95	8.0
96	6.5
97	5.0
98	5.0
99	7.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07006884945991358
2	0.011125548126652946
3	0.02158798411330671
4	0.0207038345933078
5	0.012783328476650901
6	0.009909842536654445
7	0.010462435986653763
8	0.009136211706655399
9	0.006741640089991685
10-11	0.011180807471652876
12-13	0.008602038038322725
14-15	0.009246730396655262
16-17	0.013078044983317202
18-19	0.006446923583325383
20-21	0.013501699961650016
22-23	0.005249637774993526
24-25	0.00419971021999482
26-27	0.0038313145866619414
28-29	0.010591374458320271
30-31	0.006925837906658124
32-33	0.003112943101662827
34-35	0.008325741313323065
36-37	0.006594281836658534
38-39	0.0016946199133312434
40-41	0.0012341253716651446
42-43	0.001418323188331584
44-45	0.002247213363330562
46-47	0.004162870656661532
48-49	0.0027629672499965923
50-51	0.0050286003949937975
52-53	0.005065439958327086
54-55	0.005286477338326813
56-57	0.00209985510999741
58-59	0.0028919057216630997
60-61	0.005304897119993457
62-63	0.002412991398330357
64-65	0.003518178298328994
66-67	0.0012341253716651446
68-69	0.0018603979483310389
70-71	0.0011604462449985686
72-73	0.001823558384997751
74-75	0.0022656331449972056
76-77	0.001805138603331107
78-79	2.7629672499965925E-4
80-81	1.473582533331516E-4
82-83	1.841978166664395E-5
84-85	3.6839563333287896E-4
86-87	1.6577803499979554E-4
88-89	3.68395633332879E-5
90-91	5.710132316659624E-4
92-93	0.001731459476664531
94-95	0.0013078044983317204
96-97	9.394088649988414E-4
98-99	0.0012709649349984325
100-101	0.001068347336665349
102-103	0.002210373799997274
104-105	0.004660204761660919
106-107	0.0063916642383254505
108-109	0.011880759174985347
110-111	0.009412508431655057
112-113	0.003573437643328926
114-115	0.007073196159991276
116-117	0.0024314111799970013
118-119	0.001123606681665281
120-121	0.00429180912832804
122-123	8.104703933323338E-4
124-125	0.08309163509823085
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2714473.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.3360193992887
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.48420472552453	41.216593976322166
2	13.384983244785204	14.813433849851766
3	4.974103517966707	8.257412662928278
4	2.369520693205612	5.24479372184967
5	1.3258779773259934	3.6684404737200422
6	0.8146463151039186	2.7047570577689695
7	0.5449456593247346	2.1108586503166182
8	0.370800487130998	1.6414898359317285
9	0.2742988412251705	1.3660745399314604
>10	1.359074289147701	13.180277805603016
>50	0.06787302382190567	2.5763707954870236
>100	0.028401020055825437	2.668326869479975
>500	0.0011364995520167362	0.4385608104168626
>1k	1.337058296479982E-4	0.1126089503922982
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.68395633332879E-5
2	0.0	0.0	0.0	0.0	3.68395633332879E-5
3	0.0	0.0	0.0	0.0	3.68395633332879E-5
4	0.0	0.0	0.0	0.0	3.68395633332879E-5
5	3.68395633332879E-5	0.0	0.0	0.0	3.68395633332879E-5
6	3.68395633332879E-5	0.0	0.0	0.0	1.8419781666643948E-4
7	3.68395633332879E-5	0.0	0.0	0.0	1.8419781666643948E-4
8	3.68395633332879E-5	0.0	0.0	0.0	2.210373799997274E-4
9	3.68395633332879E-5	0.0	0.0	0.0	2.210373799997274E-4
10-11	3.68395633332879E-5	0.0	0.0	0.0	2.210373799997274E-4
12-13	3.68395633332879E-5	0.0	0.0	0.0	4.973341049993867E-4
14-15	3.68395633332879E-5	0.0	0.0	0.0	6.815319216658261E-4
16-17	5.525934499993185E-5	0.0	0.0	0.0	7.736308299990458E-4
18-19	7.36791266665758E-5	0.0	0.0	3.68395633332879E-5	9.394088649988414E-4
20-21	7.36791266665758E-5	0.0	0.0	7.36791266665758E-5	0.0010130879916654173
22-23	7.36791266665758E-5	0.0	0.0	2.0261759833308345E-4	0.001086767118331993
24-25	1.105186899998637E-4	0.0	0.0	4.789143233327427E-4	0.0011604462449985688
26-27	1.105186899998637E-4	0.0	0.0	9.394088649988414E-4	0.0013262242799983643
28-29	1.2893847166650765E-4	0.0	0.0	0.0037023761149954336	0.0013630638433316522
30-31	1.473582533331516E-4	0.0	0.0	0.011954438301651923	0.0013814836249982961
32-33	1.473582533331516E-4	0.0	0.0	0.025437718481635292	0.0015472616599980916
34-35	1.473582533331516E-4	0.0	0.0	0.04286283193828047	0.0016577803499979555
36-37	1.473582533331516E-4	0.0	0.0	0.07143191330324523	0.0016577803499979555
38-39	1.473582533331516E-4	0.0	0.0	0.11976542039651895	0.0017314594766645313
40-41	1.473582533331516E-4	0.0	0.0	0.17986916797477817	0.0017314594766645313
42-43	1.473582533331516E-4	0.0	0.0	0.2328628798297128	0.001786718821664463
44-45	1.8419781666643948E-4	0.0	0.0	0.2933902823863048	0.0018603979483310389
46-47	2.7629672499965925E-4	0.0	0.0	0.3666825936378811	0.0018972375116643268
48-49	2.947165066663032E-4	0.0	0.0	0.44286681061112043	0.0019524968566642588
50-51	2.947165066663032E-4	0.0	0.0	0.5266583974126837	0.0020445957649974783
52-53	2.947165066663032E-4	0.0	0.0	0.611334133734246	0.002302472708330494
54-55	2.947165066663032E-4	0.0	0.0	0.6993622703191374	0.0024314111799970013
56-57	2.947165066663032E-4	0.0	0.0	0.8051470764306736	0.002486670524996933
58-59	2.947165066663032E-4	0.0	0.0	0.9244704220671931	0.002505090306663577
60-61	2.947165066663032E-4	0.0	0.0	1.0533904739520343	0.002505090306663577
62-63	2.947165066663032E-4	0.0	0.0	1.1830657368852076	0.002578769433330153
64-65	2.947165066663032E-4	0.0	0.0	1.320956222441704	0.0026156089966634407
66-67	2.947165066663032E-4	0.0	0.0	1.4746140411048478	0.0026340287783300845
68-69	2.947165066663032E-4	0.0	0.0	1.6522359957162958	0.002670868341663373
70-71	3.315560699995911E-4	0.0	0.0	1.844299059154392	0.002781387031663236
72-73	3.49975851666235E-4	0.0	0.0	2.0431774418091466	0.003076103538329539
74-75	3.6839563333287896E-4	0.0	0.0	2.246273954465563	0.003205042009996047
76-77	3.6839563333287896E-4	0.0	0.0	2.467035037740291	0.0032234617916626906
78-79	3.6839563333287896E-4	0.0	0.0	2.7178387849133143	0.003260301354995979
80-81	4.420747599994548E-4	0.0	0.0	2.98680443680965	0.003315560699995911
82-83	4.420747599994548E-4	0.0	0.0	3.272605769149297	0.0034076596083291305
84-85	4.420747599994548E-4	0.0	0.0	3.5590149542839438	0.0034629189533290626
86-87	4.420747599994548E-4	0.0	0.0	3.8719670448002246	0.003647116769995502
88-89	4.420747599994548E-4	0.0	0.0	4.210098976854807	0.0038865739316618735
90-91	4.789143233327427E-4	0.0	0.0	4.580870762022684	0.003904993713328517
92-93	4.789143233327427E-4	0.0	0.0	4.9696018343155375	0.004015512403328381
94-95	4.789143233327427E-4	0.0	0.0	5.363085210278386	0.004070771748328313
96-97	4.789143233327427E-4	0.0	0.0	5.769628211442884	0.004328648691661328
98-99	5.341736683326746E-4	0.0	0.0	6.208442670087343	0.004531266289994411
100-101	5.894330133326064E-4	0.0	0.0	6.6952590797550755	0.004531266289994411
102-103	5.894330133326064E-4	0.0	0.0	7.193385235366128	0.004604945416660987
104-105	6.262725766658943E-4	0.0	0.0	7.693058652637179	0.004678624543327563
106-107	6.446923583325382E-4	0.0	0.0	8.193855676589894	0.004697044324994207
108-109	6.9995170333247E-4	3.68395633332879E-5	0.0	8.721250865269244	0.004770723451660783
110-111	7.367912666657579E-4	3.68395633332879E-5	0.0	9.288561720820212	0.004862822359994003
112-113	7.736308299990458E-4	3.68395633332879E-5	0.0	9.884957411622809	0.004862822359994003
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6820	0.0	35.533424	1
TCAACGC	7540	0.0	31.884008	4
CAACGCA	7720	0.0	31.140598	5
ATCAACG	7920	0.0	30.20562	3
AACGCAG	8230	0.0	29.427235	6
TATCAAC	8260	0.0	29.106382	2
GGTATCA	4750	0.0	27.076166	1
GTACATG	12575	0.0	26.279192	1
TACATGG	12335	0.0	26.100271	2
ACGCAGA	9240	0.0	25.888624	7
ACATGGG	12240	0.0	25.62218	3
CATGGGG	7675	0.0	25.508234	4
CGCAGAG	9510	0.0	24.966364	8
GTCCGTA	190	3.289594E-5	21.936708	1
GCAGAGT	10515	0.0	21.561481	9
TATAGCG	170	3.9312587E-4	21.003382	2
CCTATAC	675	0.0	19.395716	3
GTATAAG	1025	0.0	18.588875	1
AGAGTAC	9410	0.0	18.530233	10-11
ATGGGGA	3925	0.0	18.041399	5
>>END_MODULE