FastQCFastQC Report
Mon 15 Aug 2016
SRR2921130_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921130_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2714473
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27710.10208242999654076No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58900.038.3924831
GTATCAA84000.034.9468541
TCAACGC101800.028.537454
CAACGCA103150.028.105295
ATCAACG104700.027.7470153
TATCAAC106250.027.6193852
AACGCAG108900.026.7129086
ACGCAGA125450.023.0469677
GTACATG128200.023.0377181
ACATGGG121000.022.678093
TACATGG127450.022.650792
CGCAGAG128300.022.4886448
CATGGGG76600.022.1168674
TACCGTA4600.021.985657
ACCGTAT4450.021.3898728
GTACCGT5450.020.739456
CCGTATG4453.6379788E-1220.0537419
AGTACTT67850.019.8601512-13
GCAGAGT141050.019.7817139
GAGTACT62350.019.56057412-13
Produced by FastQC (version 0.11.5)