##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921130_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2714473
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.654091972917026	31.0	31.0	34.0	27.0	34.0
2	30.767066756604322	31.0	31.0	34.0	27.0	34.0
3	30.882243440992045	31.0	31.0	34.0	27.0	34.0
4	34.38740005886962	37.0	35.0	37.0	30.0	37.0
5	34.01007819934109	37.0	35.0	37.0	30.0	37.0
6	33.87876983856535	37.0	35.0	37.0	29.0	37.0
7	33.70034072912127	37.0	35.0	37.0	28.0	37.0
8	33.69976897909834	37.0	35.0	37.0	28.0	37.0
9	35.12004687466038	39.0	35.0	39.0	28.0	39.0
10-11	35.08161086885005	39.0	35.0	39.0	28.0	39.0
12-13	34.949606608722945	39.0	35.0	39.0	27.0	39.0
14-15	36.07144941209583	40.0	36.0	41.0	27.0	41.0
16-17	36.021578774222476	40.0	36.0	41.0	27.0	41.0
18-19	36.05710998046398	40.0	36.0	41.0	27.0	41.0
20-21	36.05638976700081	40.0	36.0	41.0	27.0	41.0
22-23	36.02004551159654	40.0	36.0	41.0	27.0	41.0
24-25	35.927627756842675	40.0	36.0	41.0	27.0	41.0
26-27	35.824647730885516	40.0	35.5	41.0	27.0	41.0
28-29	35.7389635115177	40.0	35.0	41.0	27.0	41.0
30-31	35.651236538363065	39.0	35.0	41.0	27.0	41.0
32-33	35.518548535940496	39.0	35.0	41.0	26.0	41.0
34-35	35.39390463636957	39.0	35.0	41.0	25.0	41.0
36-37	35.26251375497196	39.0	35.0	41.0	25.0	41.0
38-39	35.097370465648396	39.0	35.0	41.0	25.0	41.0
40-41	34.948826162573724	39.0	34.0	41.0	24.0	41.0
42-43	34.81074982142022	39.0	34.0	41.0	24.0	41.0
44-45	34.63890431033943	39.0	34.0	41.0	23.5	41.0
46-47	34.41038739379614	39.0	34.0	41.0	22.5	41.0
48-49	34.19954831011397	38.5	33.0	41.0	20.5	41.0
50-51	33.984551513314	38.0	33.0	40.5	19.5	41.0
52-53	33.805064371610996	38.0	33.0	40.0	18.5	41.0
54-55	33.59704756687578	38.0	33.0	40.0	18.0	41.0
56-57	33.320552276629755	38.0	32.5	40.0	15.5	41.0
58-59	33.086003802579725	37.0	32.0	40.0	13.5	41.0
60-61	32.81607608548694	37.0	32.0	40.0	13.0	41.0
62-63	32.52162869183079	36.0	32.0	40.0	10.0	41.0
64-65	32.22788917038409	36.0	31.0	39.0	8.0	41.0
66-67	31.90059083291674	35.5	31.0	39.0	7.5	41.0
68-69	31.543294223224912	35.0	31.0	38.5	7.0	40.0
70-71	31.189816402668214	35.0	31.0	38.0	5.5	40.0
72-73	30.99943580208755	35.0	31.0	37.0	2.0	39.5
74-75	30.795673966917334	35.0	31.0	37.0	2.0	39.0
76-77	30.561001896132325	35.0	31.0	36.0	2.0	39.0
78-79	30.236557519636406	35.0	30.0	36.0	2.0	38.5
80-81	29.945222516488467	35.0	30.0	35.5	2.0	37.0
82-83	29.7284474739664	34.5	30.0	35.0	2.0	37.0
84-85	29.57283071152301	34.0	30.0	35.0	2.0	36.5
86-87	29.37013906566763	34.0	30.0	35.0	2.0	36.0
88-89	29.224436566508487	34.0	30.0	35.0	2.0	36.0
90-91	29.06785479170358	34.0	29.0	35.0	2.0	35.5
92-93	28.95008883860698	34.0	29.0	35.0	2.0	35.0
94-95	28.84395221466561	34.0	29.0	35.0	2.0	35.0
96-97	28.782037434153885	34.0	29.0	35.0	2.0	35.0
98-99	28.737069589566744	34.0	29.0	35.0	2.0	35.0
100-101	28.59112137052017	34.0	29.0	35.0	2.0	35.0
102-103	27.225258457166454	32.0	26.0	34.0	2.0	35.0
104-105	28.316069454365543	34.0	29.0	35.0	2.0	35.0
106-107	28.606260220676354	34.0	29.0	35.0	2.0	35.0
108-109	28.809996268152233	34.0	30.0	35.0	2.0	35.0
110-111	28.788677397049078	34.0	30.0	35.0	2.0	35.0
112-113	28.72278928543404	34.0	30.0	35.0	2.0	35.0
114-115	28.614131730173774	34.0	30.0	35.0	2.0	35.0
116-117	28.526975954448616	34.0	29.5	35.0	2.0	35.0
118-119	28.39828172908701	34.0	29.0	35.0	2.0	35.0
120-121	28.235089463037575	34.0	29.0	35.0	2.0	35.0
122-123	28.0684992998641	34.0	29.0	35.0	2.0	35.0
124-125	27.285482670116814	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	64334.0
3	22003.0
4	11061.0
5	8975.0
6	9643.0
7	11316.0
8	13333.0
9	14798.0
10	15856.0
11	17030.0
12	17971.0
13	19227.0
14	21388.0
15	18427.0
16	13435.0
17	11921.0
18	11313.0
19	10737.0
20	10666.0
21	10772.0
22	11982.0
23	14874.0
24	20409.0
25	27817.0
26	34954.0
27	40764.0
28	48662.0
29	57616.0
30	68316.0
31	81956.0
32	101607.0
33	128958.0
34	170044.0
35	245811.0
36	432000.0
37	664980.0
38	229068.0
39	449.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.44767279811352	19.03286294277005	13.67755560110841	24.84190865800802
2	16.14981220977246	19.828290289246684	38.09096820183719	25.930929299143664
3	19.2908026638187	23.63949368730081	29.24995091084784	27.819752738032655
4	13.446474184022913	16.208858837080644	35.475170536138386	34.869496442758056
5	14.18021125989509	37.04247943749555	34.07383294815311	14.703476354456251
6	33.80885028916024	35.62132885129128	16.42383003118001	14.145990828368468
7	29.02827768421355	31.493460677295282	20.508312273706494	18.969949364784668
8	26.830074353592394	35.13943186796223	19.07675688501149	18.953736893433888
9	26.421850410890862	15.49078641302205	18.961370768623656	39.12599240746343
10-11	25.6893865114992	25.64944503828523	27.15800942604029	21.50315902417528
12-13	26.09916403749165	22.898721875503767	27.497722405176976	23.504391681827602
14-15	23.34712658587244	24.36283666999353	24.879682905658978	27.41035383847505
16-17	23.53555693249221	27.434603032294568	25.5720536150391	23.457786420174124
18-19	23.462416593092208	26.432362101316087	27.346355890108498	22.75886541548321
20-21	24.288690405452687	25.547946757699552	27.197991557421858	22.9653712794259
22-23	23.871426584159465	25.36414732270763	27.06960324501304	23.694822848119866
24-25	23.548579901803997	25.68145404376812	27.194412467443644	23.57555358698424
26-27	23.43090144206631	25.812856202945007	27.145204685937085	23.611037669051594
28-29	23.412945098540654	25.88144536367164	27.266409286327825	23.43920025145988
30-31	23.625488736918268	25.791103878002275	27.130060169385196	23.453347215694265
32-33	23.386977463495935	25.79036026975046	27.00233230275239	23.820329964001214
34-35	23.42728656564953	25.897755064214458	27.16957090010786	23.505387470028154
36-37	23.694563790873737	25.697722901451264	27.055849338467947	23.551863969207055
38-39	23.62144644708277	25.84210493343313	26.985038622310615	23.551409997173483
40-41	23.629497161084153	25.799366329219904	27.092521240278998	23.47861526941695
42-43	23.747401407325754	25.673773186963988	26.897126960195173	23.681698445515092
44-45	23.671232604124672	25.78978361052991	26.847317960082812	23.69166582526261
46-47	23.83643855298527	25.841001723303396	26.644743206051846	23.677816517659483
48-49	23.762641658149995	26.058577368854802	26.450279691303198	23.728501281692008
50-51	23.666014468733838	26.273780519150534	26.365004701242185	23.69520031087344
52-53	23.88658589365622	26.038618708927707	26.305114737558345	23.769680659857727
54-55	23.721123571655994	26.091378639402834	26.354881575688964	23.8326162132522
56-57	23.66897266355446	26.24547139417217	26.225830469338877	23.859725472934493
58-59	23.601838016504562	26.018324891894927	26.406839868155114	23.972997223445397
60-61	23.663941690296024	26.139432563744	26.482716943309804	23.713908802650174
62-63	23.536596510855762	26.189218012185695	26.517849970188017	23.756335506770526
64-65	23.449373543539632	26.03957205239692	26.536097553155795	23.974956850907656
66-67	23.476557843162002	26.274555398792778	26.315387014993274	23.933499743051946
68-69	23.69407563814851	26.04546911792343	26.383309196634862	23.877146047293188
70-71	23.421114457581567	26.01507846495588	26.452709581541733	24.111097495920816
72-73	23.63726154115474	26.017699278116375	26.29038425215713	24.054654928571757
74-75	23.490997676112375	26.147429877570755	26.283808345691597	24.077764100625274
76-77	23.483389563965577	25.920408523081395	26.38406482897849	24.212137083974536
78-79	23.518452772351417	26.064954333503877	26.31653321900665	24.100059675138056
80-81	23.550594398578966	26.084556544188054	26.248386253412427	24.116462803820557
82-83	23.512223072805977	26.02277783458572	26.33696566374551	24.128033428862793
84-85	23.468936019066923	26.04305164351251	26.13509224970565	24.352920087714917
86-87	23.382429233812132	25.960535120014384	26.27774125942149	24.379294386751994
88-89	23.35369265294038	25.951767333010196	26.194472677778347	24.500067336271076
90-91	23.26535891069956	26.031315103159148	26.283510970308356	24.419815015832942
92-93	23.314368421644136	26.00804791236431	26.36473392347427	24.312849742517287
94-95	23.26994746287333	25.829393708993653	26.413313556759977	24.487345271373044
96-97	23.302668525482286	25.644562494507472	26.48106637110344	24.571702608906804
98-99	23.16387698862893	25.739399475238145	26.326928625092254	24.76979491104067
100-101	23.135033798105255	25.754320171806498	26.427647773098105	24.68299825699014
102-103	23.053932977149046	25.576275554803914	26.497262664632064	24.87252880341498
104-105	23.03607317620924	25.73684044717525	26.260593569677184	24.966492806938323
106-107	22.929259359014843	25.7068334634591	26.35754212998256	25.0063650475435
108-109	22.839301478661614	25.695448469387006	26.418843834881557	25.046406217069823
110-111	22.88326416802022	25.789810030729697	26.282284745184448	25.04464105606563
112-113	22.78221475854797	25.79118651911091	26.29249759504057	25.134101127300557
114-115	22.7243989819573	25.92641443533852	26.23173145365828	25.1174551290459
116-117	22.59475656369031	25.829889602268636	26.2743984807486	25.300955353292455
118-119	22.593523660473416	25.72109508565016	26.22925060783955	25.456130646036872
120-121	22.428855367645838	25.881707139607236	26.326544145684167	25.36289334706276
122-123	22.47536127978213	25.851033121373685	26.147889611017604	25.52571598782658
124-125	22.361283048063896	25.94437849560342	26.129840029060492	25.56449842727219
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	25.0
1	41.0
2	84.0
3	129.0
4	127.5
5	112.5
6	147.5
7	298.0
8	461.5
9	538.0
10	529.0
11	471.0
12	482.0
13	575.5
14	690.0
15	822.5
16	963.0
17	1079.0
18	1231.5
19	1456.0
20	1684.5
21	2003.0
22	2494.0
23	3094.5
24	3852.5
25	5077.5
26	6959.5
27	9380.0
28	12233.5
29	15640.0
30	19409.5
31	23804.0
32	29358.0
33	34469.0
34	38672.5
35	43898.0
36	50244.5
37	55981.0
38	60871.5
39	65796.0
40	71880.0
41	76828.5
42	80382.5
43	86001.5
44	92122.0
45	97694.0
46	102803.5
47	106540.0
48	109972.0
49	113508.5
50	115764.5
51	114694.0
52	111601.5
53	110460.5
54	109609.5
55	103497.0
56	95051.0
57	87752.0
58	80911.5
59	72866.0
60	62328.0
61	52899.5
62	43630.5
63	32364.0
64	22907.5
65	16948.0
66	12580.5
67	9225.5
68	6693.0
69	4877.0
70	3724.0
71	2787.5
72	2128.0
73	1846.0
74	1426.0
75	1038.0
76	741.5
77	444.5
78	270.0
79	166.5
80	109.0
81	67.0
82	42.5
83	30.5
84	19.0
85	8.0
86	9.0
87	10.5
88	8.5
89	6.0
90	4.5
91	3.0
92	2.5
93	3.0
94	1.5
95	2.0
96	3.0
97	1.5
98	1.5
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07798935557657048
2	2.947165066663032E-4
3	8.841495199989095E-4
4	0.18161904723310934
5	0.06962677469991413
6	0.008620457819989368
7	0.013041205419983916
8	0.010315077733320611
9	0.018125065159977645
10-11	0.018493460793310525
12-13	0.019727586164975667
14-15	0.0260271514949679
16-17	0.02650606581830064
18-19	0.024645667869969603
20-21	0.026837621888300234
22-23	0.028384883548298327
24-25	0.026413966909967425
26-27	0.025548237171635155
28-29	0.026524485599967288
30-31	0.026432386691634065
32-33	0.023264184244971307
34-35	0.024903544813302616
36-37	0.027058659268299957
38-39	0.03263985311329307
40-41	0.02803490769663209
42-43	0.02742705490163284
44-45	0.027813870316632367
46-47	0.029084835251630792
48-49	0.024958804158302553
50-51	0.030521578221629025
52-53	0.027132338394966538
54-55	0.03098207276329512
56-57	0.027684931844965854
58-59	0.024977223939969196
60-61	0.025345619573302072
62-63	0.02943481110329703
64-65	0.03376345979495836
66-67	0.032768791584959586
68-69	0.028108586823298667
70-71	0.03685798311495454
72-73	0.039989345998284014
74-75	0.02584295367830146
76-77	0.01847504101164388
78-79	0.022564232541638838
80-81	0.025032483284969126
82-83	0.023964135948303777
84-85	0.01670674197164606
86-87	0.017130396949978872
88-89	0.017775089308311412
90-91	0.018825016863310114
92-93	0.02215899734497267
94-95	0.01795928712497785
96-97	0.02057489612164129
98-99	0.017682990399978192
100-101	0.018032966251644425
102-103	0.015785752888313864
104-105	0.018548720138310458
106-107	0.015914691359980374
108-109	0.014625306643315295
110-111	0.011917598738318635
112-113	0.01068347336665349
114-115	0.009025693016655534
116-117	0.008896754544989028
118-119	0.005396996028326677
120-121	0.003978672839995093
122-123	0.007312653321657647
124-125	0.055682999978264654
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2714473.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.17341269434984
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.61633143107787	39.88047903659819
2	13.868353435390441	15.025920680930213
3	5.1069382051111125	8.299808129700796
4	2.484198787189547	5.383101044528907
5	1.3267641486603428	3.5937670886722235
6	0.8527333276672763	2.771728468676736
7	0.5498169645971973	2.0849822930635096
8	0.3773091004532045	1.6352097289748277
9	0.28981221563207604	1.4130105085170324
>10	1.4204060791242137	13.468959289438635
>50	0.07525735471088275	2.781283217119148
>100	0.030307488515578802	2.7843247596704894
>500	0.0013626629772737087	0.47702551095166174
>1k	4.08798893159081E-4	0.4004002431576539
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2771	0.10208242999654076	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.36791266665758E-5
2	0.0	0.0	0.0	0.0	7.36791266665758E-5
3	0.0	0.0	0.0	0.0	7.36791266665758E-5
4	0.0	0.0	0.0	0.0	7.36791266665758E-5
5	0.0	0.0	0.0	0.0	7.36791266665758E-5
6	0.0	0.0	0.0	0.0	1.473582533331516E-4
7	3.68395633332879E-5	0.0	0.0	0.0	1.8419781666643948E-4
8	3.68395633332879E-5	0.0	0.0	0.0	1.8419781666643948E-4
9	3.68395633332879E-5	0.0	0.0	0.0	1.8419781666643948E-4
10-11	3.68395633332879E-5	0.0	0.0	0.0	2.210373799997274E-4
12-13	3.68395633332879E-5	0.0	0.0	0.0	5.710132316659624E-4
14-15	3.68395633332879E-5	0.0	0.0	0.0	6.9995170333247E-4
16-17	3.68395633332879E-5	0.0	0.0	3.68395633332879E-5	8.841495199989096E-4
18-19	3.68395633332879E-5	0.0	0.0	7.36791266665758E-5	0.0010315077733320612
20-21	3.68395633332879E-5	0.0	0.0	1.105186899998637E-4	0.0011420264633319249
22-23	3.68395633332879E-5	0.0	0.0	2.3945716166637134E-4	0.0012893847166650764
24-25	7.36791266665758E-5	0.0	0.0	4.420747599994548E-4	0.0013814836249982961
26-27	7.36791266665758E-5	0.0	0.0	9.025693016655535E-4	0.001418323188331584
28-29	1.105186899998637E-4	0.0	0.0	0.0037023761149954336	0.001436742969998228
30-31	1.473582533331516E-4	0.0	0.0	0.012009697646651855	0.0015104220966648037
32-33	1.473582533331516E-4	0.0	0.0	0.025290360228302142	0.0016577803499979555
34-35	1.8419781666643948E-4	0.0	0.0	0.04207078132661478	0.0017130396949978873
36-37	1.8419781666643948E-4	0.0	0.0	0.06975571317158064	0.0017498792583311752
38-39	1.8419781666643948E-4	0.0	0.0	0.11654195860485628	0.0017682990399978192
40-41	1.8419781666643948E-4	0.0	0.0	0.1756141984097834	0.0017682990399978192
42-43	1.8419781666643948E-4	0.0	0.0	0.22796321790638552	0.001823558384997751
44-45	2.578769433330153E-4	0.0	0.0	0.28738543356297885	0.0019340770749976146
46-47	2.947165066663032E-4	0.0	0.0	0.35959097769622317	0.0019893364199975467
48-49	2.947165066663032E-4	0.0	0.0	0.4343937110444642	0.0020998551099974103
50-51	2.947165066663032E-4	0.0	0.0	0.5172090494176954	0.002247213363330562
52-53	2.947165066663032E-4	0.0	0.0	0.6010558955642586	0.0023208924899971377
54-55	2.947165066663032E-4	0.0	0.0	0.6885498584808174	0.0024314111799970013
56-57	2.947165066663032E-4	0.0	0.0	0.79188483363069	0.0024314111799970013
58-59	2.947165066663032E-4	0.0	0.0	0.9095503989172116	0.0024498309616636455
60-61	2.947165066663032E-4	0.0	0.0	1.0361495583120555	0.002468250743330289
62-63	2.947165066663032E-4	0.0	0.0	1.163540768318565	0.002578769433330153
64-65	2.947165066663032E-4	0.0	0.0	1.2990919416033977	0.0026892881233300165
66-67	2.947165066663032E-4	0.0	0.0	1.4501525710515448	0.0027261276866633044
68-69	3.315560699995911E-4	0.0	0.0	1.6247536814696628	0.0027261276866633044
70-71	3.49975851666235E-4	0.0	0.0	1.813040689666097	0.002781387031663236
72-73	3.6839563333287896E-4	0.0	0.0	2.0090824259441886	0.0030392639749962517
74-75	3.6839563333287896E-4	0.0	0.0	2.2102448615256076	0.003186622228329403
76-77	3.6839563333287896E-4	0.0	0.0	2.4286113731836716	0.003297140918329267
78-79	3.6839563333287896E-4	0.0	0.0	2.6768731904866985	0.0033892398266624868
80-81	3.868154149995229E-4	0.0	0.0	2.9440705433430354	0.0034076596083291305
82-83	4.420747599994548E-4	0.0	0.0	3.22692471061602	0.00368395633332879
84-85	4.420747599994548E-4	0.0	0.0	3.5105340889373373	0.0037023761149954336
86-87	4.420747599994548E-4	0.0	0.0	3.8201890385352884	0.003923413494995162
88-89	4.420747599994548E-4	0.0	0.0	4.1551159285798756	0.004126031093328245
90-91	5.157538866660306E-4	0.0	0.0	4.520288100121091	0.004126031093328245
92-93	5.894330133326064E-4	0.0	0.0	4.902480149922287	0.00438390803666126
94-95	5.894330133326064E-4	0.0	0.0	5.290290233131809	0.00438390803666126
96-97	5.894330133326064E-4	0.0	0.0	5.690699447001315	0.004733883888327494
98-99	5.894330133326064E-4	0.0	0.0	6.124577404159114	0.004954921268327223
100-101	6.078527949992503E-4	0.0	0.0	6.6064388925585185	0.005010180613327154
102-103	6.446923583325382E-4	0.0	0.0	7.099536447774577	0.005120699303327017
104-105	7.367912666657579E-4	0.0	0.0	7.595139093297299	0.0052312179933268815
106-107	8.104703933323337E-4	0.0	0.0	8.093136310436686	0.005268057556660169
108-109	8.288901749989776E-4	0.0	0.0	8.617492235141038	0.005323316901660101
110-111	8.473099566656217E-4	0.0	0.0	9.182647976237007	0.005396996028326677
112-113	8.473099566656217E-4	0.0	0.0	9.77639121847961	0.005452255373326609
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5890	0.0	38.392483	1
GTATCAA	8400	0.0	34.946854	1
TCAACGC	10180	0.0	28.53745	4
CAACGCA	10315	0.0	28.10529	5
ATCAACG	10470	0.0	27.747015	3
TATCAAC	10625	0.0	27.619385	2
AACGCAG	10890	0.0	26.712908	6
ACGCAGA	12545	0.0	23.046967	7
GTACATG	12820	0.0	23.037718	1
ACATGGG	12100	0.0	22.67809	3
TACATGG	12745	0.0	22.65079	2
CGCAGAG	12830	0.0	22.488644	8
CATGGGG	7660	0.0	22.116867	4
TACCGTA	460	0.0	21.98565	7
ACCGTAT	445	0.0	21.389872	8
GTACCGT	545	0.0	20.73945	6
CCGTATG	445	3.6379788E-12	20.053741	9
AGTACTT	6785	0.0	19.86015	12-13
GCAGAGT	14105	0.0	19.781713	9
GAGTACT	6235	0.0	19.560574	12-13
>>END_MODULE