FastQCFastQC Report
Mon 15 Aug 2016
SRR2921129_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921129_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3047755
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44600.045.3815961
GTATCAA70150.043.1942371
TCAACGC85800.034.538194
CAACGCA88500.033.5528225
ATCAACG89500.033.175753
TATCAAC90450.032.8273052
AACGCAG91700.032.379826
ACGCAGA106950.027.651517
CGCAGAG111800.026.3450818
GTACATG137900.025.340171
TACATGG140000.024.1414622
CATGGGG103100.023.8368514
ACATGGG140900.023.7761023
GCAGAGT122500.022.878159
AGAGTAC108850.020.31163610-11
CAGAGTA119050.019.44619810-11
ATGGGGG49200.018.5053845
GAGTACA110200.015.95765412-13
GTCTTAG13200.015.7844641
AGTACAT107950.015.73898112-13
Produced by FastQC (version 0.11.5)