##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921129_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3047755
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.360076515336697	31.0	30.0	34.0	23.0	34.0
2	29.381263585819728	31.0	30.0	34.0	23.0	34.0
3	29.42659301682714	31.0	30.0	34.0	23.0	34.0
4	32.81411990137003	35.0	33.0	37.0	25.0	37.0
5	32.88500387990504	35.0	33.0	37.0	26.0	37.0
6	32.864316521505174	37.0	33.0	37.0	25.0	37.0
7	32.74716110710999	37.0	33.0	37.0	25.0	37.0
8	32.69048069808761	36.0	33.0	37.0	25.0	37.0
9	34.01456317847071	38.0	34.0	39.0	25.0	39.0
10-11	33.97566831979604	38.0	34.0	39.0	25.0	39.0
12-13	33.99580806199974	38.0	34.0	39.0	25.0	39.0
14-15	35.01354538012406	39.0	34.0	41.0	24.0	41.0
16-17	34.92922068867084	39.0	34.0	41.0	23.5	41.0
18-19	35.00387449122387	39.0	34.0	41.0	24.0	41.0
20-21	35.025658722567925	39.0	34.0	41.0	24.0	41.0
22-23	35.006394378813255	39.0	34.0	41.0	24.0	41.0
24-25	34.91764593938817	39.0	34.0	41.0	23.5	41.0
26-27	34.86183617121455	39.0	34.0	41.0	23.5	41.0
28-29	34.719005956843645	39.0	34.0	41.0	22.5	41.0
30-31	34.620262947645074	39.0	34.0	41.0	22.0	41.0
32-33	34.51845571576456	39.0	34.0	41.0	21.5	41.0
34-35	34.40952734061629	39.0	34.0	41.0	21.0	41.0
36-37	34.274900049380605	39.0	33.0	41.0	19.5	41.0
38-39	34.12077873713602	39.0	33.0	41.0	18.0	41.0
40-41	34.00119071250806	38.5	33.0	41.0	18.0	41.0
42-43	33.90993140852857	38.0	33.0	41.0	17.0	41.0
44-45	33.73717982580621	38.0	33.0	40.5	15.5	41.0
46-47	33.495842841698234	38.0	33.0	40.0	14.5	41.0
48-49	33.34780403936668	38.0	32.5	40.0	12.0	41.0
50-51	33.170179853695586	38.0	32.0	40.0	9.0	41.0
52-53	32.94576335696274	38.0	32.0	40.0	8.0	41.0
54-55	32.68237292695771	37.0	32.0	40.0	7.5	41.0
56-57	32.400861945924134	37.0	31.0	40.0	6.0	41.0
58-59	32.16002286929232	36.5	31.0	40.0	2.0	41.0
60-61	31.84846797068662	36.0	31.0	39.5	2.0	41.0
62-63	31.561245244450426	35.5	30.5	39.0	2.0	41.0
64-65	31.221149829956804	35.0	30.0	39.0	2.0	41.0
66-67	30.92952730780525	35.0	30.0	38.5	2.0	40.0
68-69	30.568024168609355	35.0	29.5	38.0	2.0	40.0
70-71	30.252535718914416	35.0	29.0	37.0	2.0	40.0
72-73	29.902300381756408	34.5	29.0	37.0	2.0	39.0
74-75	29.83231526156138	35.0	29.0	36.0	2.0	39.0
76-77	29.602318591881563	34.5	29.0	36.0	2.0	39.0
78-79	29.36372165741669	34.0	29.0	35.5	2.0	37.5
80-81	29.034690616535777	34.0	29.0	35.0	2.0	37.0
82-83	28.808692299741942	34.0	29.0	35.0	2.0	36.5
84-85	28.654114421926963	34.0	29.0	35.0	2.0	36.0
86-87	28.4668218081834	34.0	29.0	35.0	2.0	36.0
88-89	28.327913661039027	34.0	29.0	35.0	2.0	35.5
90-91	28.185164161817468	34.0	28.5	35.0	2.0	35.0
92-93	28.387790357164533	34.0	29.0	35.0	2.0	35.0
94-95	28.38101569844033	34.0	29.0	35.0	2.0	35.0
96-97	28.32839663949366	34.0	29.0	35.0	2.0	35.0
98-99	28.229972389512938	34.0	29.0	35.0	2.0	35.0
100-101	28.136618921140315	34.0	29.0	35.0	2.0	35.0
102-103	26.846962436285068	32.5	26.0	34.0	2.0	35.0
104-105	27.8536842692408	34.0	28.0	35.0	2.0	35.0
106-107	28.026122998731854	34.0	29.0	35.0	2.0	35.0
108-109	27.967712627819495	34.0	29.0	35.0	2.0	35.0
110-111	27.94429096170788	34.0	29.0	35.0	2.0	35.0
112-113	27.868291086389817	34.0	29.0	35.0	2.0	35.0
114-115	27.742452559342862	34.0	29.0	35.0	2.0	35.0
116-117	27.589382840812334	34.0	27.5	35.0	2.0	35.0
118-119	27.478448398903453	34.0	27.0	35.0	2.0	35.0
120-121	27.305145754826093	34.0	27.0	35.0	2.0	35.0
122-123	27.16367752657284	34.0	27.0	35.0	2.0	35.0
124-125	26.123003325398532	33.0	23.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	139316.0
3	19765.0
4	13001.0
5	11837.0
6	13185.0
7	14516.0
8	15645.0
9	16831.0
10	18151.0
11	19692.0
12	20094.0
13	20197.0
14	20243.0
15	17932.0
16	14270.0
17	12574.0
18	11536.0
19	11212.0
20	11403.0
21	12297.0
22	15036.0
23	20426.0
24	28629.0
25	36763.0
26	42581.0
27	48919.0
28	57403.0
29	67265.0
30	79175.0
31	95397.0
32	118610.0
33	151305.0
34	202314.0
35	293767.0
36	496095.0
37	672853.0
38	187436.0
39	84.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.227998668287306	19.118942037008217	13.83595637393284	24.81710292077163
2	16.556355498385532	19.910285354264563	38.172695770876544	25.36066337647337
3	19.395017209647904	23.933257808664422	28.684781210372524	27.986943771315154
4	14.110023356227927	16.53353146363179	34.61874083775905	34.73770434238123
5	15.33269716177353	36.45922617211547	32.856215601779354	15.351861064331645
6	33.32917686914554	34.35064981942351	17.192503138677363	15.127670172753591
7	28.86254201036797	31.601214387578906	20.63510554028237	18.901138061770745
8	26.829906159331596	33.98959673910404	19.6488210359553	19.53167606560906
9	26.71687293543919	16.295752570662245	19.148263152074723	37.83911134182384
10-11	25.809630925806974	25.76264039528487	26.562562296498104	21.865166382410052
12-13	26.102150691999938	23.493215357140222	26.564216823092313	23.840417127767527
14-15	23.83224675108188	24.732040547653206	24.097006834508296	27.33870586675662
16-17	23.946900758544185	27.435423001062727	24.896988383843606	23.720687856549485
18-19	23.883672145477743	26.70671839331084	26.235685674994315	23.173923786217102
20-21	25.06297340809197	25.60702558774634	25.904139312011417	23.425861692150278
22-23	24.838643628662798	25.24799826224028	25.62575428156301	24.28760382753391
24-25	24.27324811159927	25.65168507803716	25.745906188717516	24.329160621646047
26-27	23.988102933349783	25.885225364396476	25.9135587447607	24.21311295749304
28-29	23.985062096061398	25.98803806044827	26.022805226264683	24.004094617225647
30-31	24.31190003873615	25.77104389588916	25.81279887830859	24.104257187066093
32-33	24.154327430559086	25.673934421227017	25.667421233909394	24.504316914304503
34-35	24.321141838230396	25.78551911447619	25.740236199621524	24.153102847671885
36-37	24.32336387418449	25.66803087390476	25.78427150267058	24.22433374924017
38-39	24.13297026220394	25.881778545423046	25.834168935040935	24.151082257332078
40-41	24.294934084148057	25.789694989174667	25.896151915953986	24.01921901072329
42-43	24.25235118306433	25.66281493614409	25.790959868045665	24.293874012745917
44-45	24.17653972453873	25.741676969558565	25.690326568861376	24.391456737041324
46-47	24.24371933783924	25.81818945452177	25.711253848689235	24.226837358949755
48-49	24.15947716149854	25.990481585695836	25.633897879085723	24.216143373719902
50-51	24.13583497807287	26.12940695860178	25.62382796926757	24.11093009405777
52-53	24.25915328616607	25.923548215411895	25.5981767912655	24.21912170715653
54-55	24.197899499853573	25.857791726788964	25.617865817694703	24.32644295566276
56-57	24.185252850187716	25.953852322444444	25.52272308177921	24.33817174558863
58-59	24.131383366968244	25.846001043745947	25.672491814666028	24.35012377461978
60-61	24.2389202558988	25.927849715728158	25.75023518421478	24.08299484415826
62-63	24.06808863610599	25.98054680708192	25.695034033326063	24.25633052348602
64-65	24.12291495223208	25.81751459812341	25.710087634677446	24.349482814967065
66-67	24.062199109927278	25.957118555671872	25.602346140740558	24.378336193660292
68-69	24.151149614520218	25.912037472247473	25.650594030801305	24.286218882431005
70-71	24.021313634239096	25.858686844807455	25.82623636255359	24.29376315839986
72-73	24.13989408048682	25.89044855753937	25.674284946491465	24.29537241548234
74-75	24.101162619389722	26.02209478860526	25.625466132184833	24.251276459820183
76-77	24.110389017009414	25.832067485766704	25.66510521313734	24.392438284086545
78-79	24.062123236218476	25.88889798485669	25.719362998620127	24.329615780304707
80-81	24.01542579833034	26.037883994151095	25.651615625486528	24.295074582032033
82-83	24.115660253419403	25.918676507355908	25.704010231796925	24.261653007427764
84-85	24.091072102958947	25.882318006889697	25.640729078178936	24.385880811972417
86-87	24.019455689548934	25.875384628509067	25.782381471421832	24.32277821052017
88-89	23.995174151986323	25.785885969489996	25.734241389849476	24.4846984886742
90-91	23.976424463343356	25.841382889033515	25.719735193556005	24.462457454067128
92-93	23.95781621026403	25.886361928673686	25.867282035599832	24.288539825462454
94-95	23.963728933626907	25.777380686569312	25.872008848586248	24.386881531217533
96-97	23.967466315542353	25.68200270791393	25.90951603872189	24.44101493782183
98-99	23.89695767506087	25.81519585880389	25.779661080903633	24.50818538523161
100-101	23.836911996243746	25.860591035944452	25.892008000087934	24.410488967723868
102-103	23.686107993541995	25.804498257123466	25.971198200342783	24.538195548991755
104-105	23.68726854043132	25.99021178166016	25.84276999590011	24.47974968200841
106-107	23.720325639687474	25.944074381115772	25.82025569944102	24.515344279755734
108-109	23.60955767539258	26.026720972063096	25.799679175076	24.564042177468327
110-111	23.606890894175553	26.157309269893354	25.790631665299422	24.445168170631664
112-113	23.60145507569455	26.091597976360028	25.88314409354142	24.423802854404002
114-115	23.574033695753823	26.29382802335635	25.756005204154793	24.376133076735034
116-117	23.539922111679736	26.258860378836875	25.723784221331712	24.477433288151676
118-119	23.65784790728433	26.197478677958802	25.686573253866445	24.45810016089042
120-121	23.54169233563344	26.249726630630853	25.79100749717033	24.417573536565378
122-123	23.614141143033947	26.26450628352699	25.718099792927916	24.403252780511142
124-125	23.62313027193164	26.419009879661253	25.557751546657677	24.40010830174943
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	483.0
1	408.0
2	292.5
3	224.0
4	173.5
5	167.0
6	180.0
7	228.0
8	287.5
9	308.5
10	324.0
11	338.0
12	365.5
13	424.0
14	491.5
15	555.0
16	656.5
17	762.5
18	882.5
19	1019.5
20	1175.5
21	1419.0
22	1720.0
23	2188.0
24	2738.5
25	3477.5
26	4718.5
27	6592.0
28	8817.5
29	11924.0
30	16108.0
31	20821.0
32	26348.0
33	31645.0
34	36625.0
35	42316.0
36	49269.5
37	56213.5
38	62356.5
39	68424.5
40	74639.0
41	80971.0
42	87176.0
43	95436.0
44	103816.0
45	110450.0
46	117643.0
47	124017.5
48	128914.0
49	133156.5
50	134069.5
51	133371.5
52	131596.0
53	129934.0
54	128520.5
55	122506.0
56	113043.0
57	102339.5
58	93419.5
59	84997.0
60	74387.0
61	63921.5
62	53227.0
63	40732.0
64	30148.5
65	22900.0
66	17139.5
67	12932.5
68	9676.5
69	7199.5
70	5625.0
71	4337.0
72	3186.0
73	2467.5
74	1937.5
75	1437.5
76	992.5
77	630.5
78	417.5
79	261.5
80	203.0
81	157.5
82	102.0
83	83.5
84	70.0
85	50.0
86	41.5
87	30.0
88	29.0
89	28.0
90	19.5
91	14.5
92	12.0
93	11.0
94	9.5
95	10.0
96	11.0
97	10.5
98	14.0
99	14.0
100	20.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06736105756532268
2	0.010335476440855645
3	0.020572519772750764
4	0.019752243864746347
5	0.011779162038943419
6	0.00961363364181176
7	0.00961363364181176
8	0.008169948043723986
9	0.0063653410461142704
10-11	0.010909669576458739
12-13	0.008120731489243723
14-15	0.009154279133329286
16-17	0.013272064191511456
18-19	0.006430963118754624
20-21	0.014338422871917198
22-23	0.005020088556987028
24-25	0.004478706457704113
26-27	0.003707647104179962
28-29	0.011139346830699974
30-31	0.007037967290677892
32-33	0.0030186153414562523
34-35	0.008137137007403811
36-37	0.00648017967323489
38-39	0.0016733628523290093
40-41	0.0014272800799276844
42-43	0.0011155752348860064
44-45	0.002198339433451836
46-47	0.004150596094502346
48-49	0.002739721532734751
50-51	0.005249765811228265
52-53	0.005282576847548442
54-55	0.004839627857226057
56-57	0.002001473215530776
58-59	0.0025756663511338675
60-61	0.004724789230105438
62-63	0.002641288423774221
64-65	0.0033795367409781953
66-67	8.694924624846812E-4
68-69	0.001952256661050511
70-71	0.0011319807530460945
72-73	0.002083500806331218
74-75	0.002231150469772013
76-77	0.0019850676973706878
78-79	2.1327173608114825E-4
80-81	1.1483862712061831E-4
82-83	3.281103632017666E-5
84-85	5.249765811228265E-4
86-87	2.952993268815899E-4
88-89	1.640551816008833E-5
90-91	6.398152082434448E-4
92-93	0.0014436855980877728
94-95	0.001197602825686448
96-97	7.05437280883798E-4
98-99	0.001197602825686448
100-101	0.0012632248983268013
102-103	0.002083500806331218
104-105	0.003658430549699697
106-107	0.005036494075147117
108-109	0.009794094341572732
110-111	0.008366814261645046
112-113	0.0025756663511338675
114-115	0.006234096900833564
116-117	0.001903040106570246
118-119	9.351145351250347E-4
120-121	0.0045443285303444665
122-123	7.874648716842396E-4
124-125	0.08397984746149215
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	3047755.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.119694249458576
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.21748166343664	43.53519249079217
2	12.766916865139033	14.584847756899952
3	4.503944355323822	7.717917734780146
4	2.0226161710027677	4.621248690867548
5	1.1426295458781925	3.263332515048003
6	0.7049687207165151	2.416055866965558
7	0.5025265869903474	2.009291550077881
8	0.35227461550433586	1.6097454663562594
9	0.26149306992163324	1.3442763782048384
>10	1.4398280947463802	14.65112420807918
>50	0.06792144527850223	2.6237840697967405
>100	0.017053139648500158	1.460099464335629
>500	2.304842755117622E-4	0.09083982028973389
>1k	1.1524213774340333E-4	0.07224398750631515
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	3.281103632017665E-5
6	0.0	0.0	0.0	0.0	3.281103632017665E-5
7	0.0	0.0	0.0	3.281103632017665E-5	3.281103632017665E-5
8	0.0	0.0	0.0	3.281103632017665E-5	6.56220726403533E-5
9	0.0	0.0	0.0	1.312441452807066E-4	6.56220726403533E-5
10-11	0.0	0.0	0.0	1.312441452807066E-4	6.56220726403533E-5
12-13	0.0	0.0	0.0	1.312441452807066E-4	1.312441452807066E-4
14-15	0.0	0.0	0.0	1.6405518160088328E-4	2.1327173608114825E-4
16-17	0.0	0.0	0.0	2.1327173608114825E-4	2.296772542412366E-4
18-19	0.0	0.0	0.0	2.7889380872150155E-4	2.952993268815899E-4
20-21	0.0	0.0	0.0	3.445158813618549E-4	2.952993268815899E-4
22-23	0.0	0.0	0.0	5.249765811228264E-4	2.952993268815899E-4
24-25	0.0	0.0	0.0	7.54653835364063E-4	2.952993268815899E-4
26-27	0.0	0.0	0.0	0.0011319807530460945	2.952993268815899E-4
28-29	0.0	0.0	0.0	0.002887371196175546	2.952993268815899E-4
30-31	0.0	0.0	0.0	0.009646444678131937	2.952993268815899E-4
32-33	0.0	0.0	0.0	0.024887171048853993	3.2811036320176657E-4
34-35	0.0	0.0	0.0	0.04774005784585703	3.609213995219432E-4
36-37	0.0	0.0	0.0	0.08058390520235387	3.609213995219432E-4
38-39	0.0	0.0	0.0	0.12766774232180736	3.9373243584211987E-4
40-41	0.0	0.0	0.0	0.17829517136383993	4.265434721622965E-4
42-43	0.0	0.0	0.0	0.23308960201853496	4.593545084824732E-4
44-45	0.0	0.0	0.0	0.3056676143587657	4.593545084824732E-4
46-47	0.0	0.0	0.0	0.38562810987103624	4.593545084824732E-4
48-49	0.0	0.0	0.0	0.462291096233129	5.249765811228264E-4
50-51	0.0	0.0	0.0	0.5370838535249717	5.577876174430031E-4
52-53	0.0	0.0	0.0	0.6190786332890932	5.905986537631798E-4
54-55	0.0	0.0	0.0	0.723056807387733	6.398152082434448E-4
56-57	0.0	0.0	0.0	0.8409140498498074	6.726262445636215E-4
58-59	0.0	3.281103632017665E-5	0.0	0.9596079737380465	7.382483172039747E-4
60-61	0.0	3.281103632017665E-5	0.0	1.080598670168698	7.546538353640631E-4
62-63	0.0	3.281103632017665E-5	0.0	1.207429731064341	7.546538353640631E-4
64-65	0.0	3.281103632017665E-5	0.0	1.3603947823890044	7.546538353640631E-4
66-67	0.0	3.281103632017665E-5	0.0	1.5362619370651514	8.03870389844328E-4
68-69	0.0	3.281103632017665E-5	0.0	1.7208076108479848	8.202759080044163E-4
70-71	0.0	3.281103632017665E-5	0.0	1.9087000103354765	8.53086944324593E-4
72-73	0.0	3.281103632017665E-5	0.0	2.1012843880167535	9.679255714452114E-4
74-75	0.0	3.281103632017665E-5	0.0	2.3264337192458058	9.843310896052997E-4
76-77	0.0	3.281103632017665E-5	0.0	2.584098787468153	9.843310896052997E-4
78-79	0.0	3.281103632017665E-5	0.0	2.857053798615702	0.001000736607765388
80-81	0.0	3.281103632017665E-5	0.0	3.132075905051423	0.0010499531622456528
82-83	0.0	3.281103632017665E-5	0.0	3.415842152666471	0.0011319807530460945
84-85	1.6405518160088326E-5	3.281103632017665E-5	0.0	3.7296633095508005	0.0011483862712061829
86-87	3.281103632017665E-5	3.281103632017665E-5	0.0	4.083940474217908	0.0011647917893662712
88-89	3.281103632017665E-5	3.281103632017665E-5	0.0	4.473128581529683	0.0011811973075263596
90-91	3.281103632017665E-5	3.281103632017665E-5	0.0	4.86458065034755	0.0011811973075263596
92-93	3.281103632017665E-5	3.281103632017665E-5	0.0	5.262693359538414	0.0011811973075263596
94-95	3.281103632017665E-5	3.281103632017665E-5	0.0	5.691238304916242	0.0012304138620066246
96-97	3.281103632017665E-5	3.281103632017665E-5	0.0	6.167621741248887	0.001296035934646978
98-99	3.281103632017665E-5	3.281103632017665E-5	0.0	6.6754381503762605	0.0013124414528070663
100-101	3.281103632017665E-5	3.281103632017665E-5	0.0	7.198331230692756	0.0013124414528070663
102-103	3.281103632017665E-5	3.281103632017665E-5	0.0	7.7133332567742485	0.0013124414528070663
104-105	3.281103632017665E-5	3.281103632017665E-5	0.0	8.255338765747247	0.0013124414528070663
106-107	3.281103632017665E-5	3.281103632017665E-5	0.0	8.835979269987252	0.0013124414528070663
108-109	3.281103632017665E-5	3.281103632017665E-5	0.0	9.460537346341816	0.0013124414528070663
110-111	3.281103632017665E-5	3.281103632017665E-5	0.0	10.098564353105811	0.0013124414528070663
112-113	3.281103632017665E-5	3.281103632017665E-5	0.0	10.732440763775305	0.0013124414528070663
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4460	0.0	45.381596	1
GTATCAA	7015	0.0	43.194237	1
TCAACGC	8580	0.0	34.53819	4
CAACGCA	8850	0.0	33.552822	5
ATCAACG	8950	0.0	33.17575	3
TATCAAC	9045	0.0	32.827305	2
AACGCAG	9170	0.0	32.37982	6
ACGCAGA	10695	0.0	27.65151	7
CGCAGAG	11180	0.0	26.345081	8
GTACATG	13790	0.0	25.34017	1
TACATGG	14000	0.0	24.141462	2
CATGGGG	10310	0.0	23.836851	4
ACATGGG	14090	0.0	23.776102	3
GCAGAGT	12250	0.0	22.87815	9
AGAGTAC	10885	0.0	20.311636	10-11
CAGAGTA	11905	0.0	19.446198	10-11
ATGGGGG	4920	0.0	18.505384	5
GAGTACA	11020	0.0	15.957654	12-13
GTCTTAG	1320	0.0	15.784464	1
AGTACAT	10795	0.0	15.738981	12-13
>>END_MODULE