FastQCFastQC Report
Mon 15 Aug 2016
SRR2921129_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921129_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3047755
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35850.1176275652078333No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31920.10473282793400389No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54300.057.998691
GTATCAA83350.043.223051
TCAACGC109900.032.499564
ATCAACG113000.031.7129923
TATCAAC113750.031.7104432
CAACGCA113950.031.3421485
AACGCAG119400.029.8440466
ACGCAGA138650.025.5289127
GTACATG135600.024.9406341
TACATGG139050.024.1399152
CGCAGAG145900.024.0572628
ACATGGG137900.023.305893
CATGGGG99350.022.9868374
GCAGAGT152950.022.444219
GAGTACT68950.021.5281712-13
AGAGTAC138750.021.4822210-11
CAGAGTA149800.021.26777610-11
GTACTTT70350.020.63530214-15
AGTACTT74150.019.57715412-13
GTGGTAT30800.018.2037661
Produced by FastQC (version 0.11.5)