##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921129_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3047755
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.54096343045947	31.0	31.0	34.0	27.0	34.0
2	30.700963824191906	31.0	31.0	34.0	27.0	34.0
3	30.781169746255852	31.0	31.0	34.0	27.0	34.0
4	34.28449202773845	37.0	35.0	37.0	30.0	37.0
5	33.903167085280806	37.0	35.0	37.0	28.0	37.0
6	33.76071272133095	37.0	35.0	37.0	29.0	37.0
7	33.57964239251515	37.0	35.0	37.0	28.0	37.0
8	33.57785025371134	37.0	35.0	37.0	28.0	37.0
9	34.99419736822678	39.0	35.0	39.0	28.0	39.0
10-11	34.9507534234215	39.0	35.0	39.0	27.0	39.0
12-13	34.81905140012894	39.0	35.0	39.0	27.0	39.0
14-15	35.90234746559353	40.0	36.0	41.0	27.0	41.0
16-17	35.840018144503084	39.5	36.0	41.0	27.0	41.0
18-19	35.88758299141499	40.0	36.0	41.0	27.0	41.0
20-21	35.888542222061815	40.0	36.0	41.0	27.0	41.0
22-23	35.84638873531501	40.0	36.0	41.0	27.0	41.0
24-25	35.73868929753212	40.0	35.0	41.0	27.0	41.0
26-27	35.63389806595347	39.0	35.0	41.0	26.5	41.0
28-29	35.542605622827296	39.0	35.0	41.0	26.0	41.0
30-31	35.45342079661915	39.0	35.0	41.0	25.5	41.0
32-33	35.30771354652851	39.0	35.0	41.0	25.0	41.0
34-35	35.17865904575663	39.0	35.0	41.0	25.0	41.0
36-37	35.04367903588051	39.0	34.5	41.0	24.5	41.0
38-39	34.87267119568338	39.0	34.0	41.0	24.0	41.0
40-41	34.72321610496907	39.0	34.0	41.0	23.5	41.0
42-43	34.579758215473355	39.0	34.0	41.0	23.0	41.0
44-45	34.3878049580757	38.5	33.5	41.0	22.5	41.0
46-47	34.142528188781576	38.0	33.0	41.0	20.5	41.0
48-49	33.927632798568126	38.0	33.0	40.5	18.5	41.0
50-51	33.69918316925081	38.0	33.0	40.0	17.5	41.0
52-53	33.505436624663076	38.0	33.0	40.0	15.5	41.0
54-55	33.282022176979446	38.0	32.5	40.0	13.5	41.0
56-57	32.995966867415525	37.0	32.0	40.0	12.5	41.0
58-59	32.75201238288511	37.0	32.0	40.0	9.5	41.0
60-61	32.46925376219545	36.5	31.5	40.0	8.0	41.0
62-63	32.15959353688207	36.0	31.0	39.5	7.5	41.0
64-65	31.85306184388181	35.5	31.0	39.0	6.5	41.0
66-67	31.515996200481993	35.0	31.0	39.0	2.0	40.5
68-69	31.15088844083596	35.0	30.5	38.0	2.0	40.0
70-71	30.800125338158743	35.0	30.0	37.0	2.0	40.0
72-73	30.611179376295013	35.0	30.0	37.0	2.0	39.0
74-75	30.41502089242738	35.0	30.0	36.5	2.0	39.0
76-77	30.195668943205735	35.0	30.0	36.0	2.0	39.0
78-79	29.889131672329306	35.0	30.0	36.0	2.0	37.5
80-81	29.609861849131573	34.5	30.0	35.0	2.0	37.0
82-83	29.413405605109332	34.0	29.5	35.0	2.0	36.5
84-85	29.270878236603664	34.0	30.0	35.0	2.0	36.0
86-87	29.08191127567669	34.0	29.0	35.0	2.0	36.0
88-89	28.95017004319573	34.0	29.0	35.0	2.0	36.0
90-91	28.807918123339967	34.0	29.0	35.0	2.0	35.0
92-93	28.702513325382128	34.0	29.0	35.0	2.0	35.0
94-95	28.60648772621159	34.0	29.0	35.0	2.0	35.0
96-97	28.545316306592888	34.0	29.0	35.0	2.0	35.0
98-99	28.50998948406286	34.0	29.0	35.0	2.0	35.0
100-101	28.373786606863085	34.0	29.0	35.0	2.0	35.0
102-103	27.013103415464826	32.0	26.0	34.0	2.0	35.0
104-105	28.104864564244828	34.0	28.5	35.0	2.0	35.0
106-107	28.39407170195767	34.0	29.0	35.0	2.0	35.0
108-109	28.604427193130682	34.0	29.0	35.0	2.0	35.0
110-111	28.58954607571803	34.0	29.0	35.0	2.0	35.0
112-113	28.52234054246486	34.0	29.5	35.0	2.0	35.0
114-115	28.415810818126786	34.0	29.0	35.0	2.0	35.0
116-117	28.330952619223	34.0	29.0	35.0	2.0	35.0
118-119	28.196266104066765	34.0	29.0	35.0	2.0	35.0
120-121	28.037218706884246	34.0	29.0	35.0	2.0	35.0
122-123	27.875235706282165	34.0	29.0	35.0	2.0	35.0
124-125	27.098990240357246	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	77092.0
3	26666.0
4	13344.0
5	10823.0
6	11834.0
7	13889.0
8	16114.0
9	17366.0
10	18729.0
11	19697.0
12	20793.0
13	22419.0
14	24579.0
15	21128.0
16	15167.0
17	13590.0
18	13128.0
19	12774.0
20	12152.0
21	12618.0
22	14067.0
23	17487.0
24	24131.0
25	32248.0
26	39406.0
27	46730.0
28	55466.0
29	65847.0
30	78051.0
31	94145.0
32	116053.0
33	146745.0
34	193916.0
35	284175.0
36	496617.0
37	726718.0
38	221839.0
39	212.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.513430555984236	19.544819633310325	14.48226541429077	24.459484396414666
2	17.097700630859038	20.538896083634185	37.084615563814474	25.278787721692304
3	19.156396194681033	24.162476121603788	29.459132588342683	27.2219950953725
4	14.399093529564327	17.10088559689612	34.30206279878335	34.197958074756194
5	15.149297443110557	36.2819184353712	33.13502304207987	15.433761079438375
6	33.072252606350006	34.68553001990838	16.980824364938552	15.261393008803065
7	28.66510684658196	31.51104562637824	20.75645804893416	19.06738947810564
8	26.82904419304823	33.919817550883124	19.54535386432808	19.70578439174057
9	26.573451128789255	15.74309292817052	19.250145133094186	38.433310809946036
10-11	25.91053604339174	25.358425526775918	26.73012021464839	22.000918215183958
12-13	26.448859855856917	23.03111023452664	26.851920980722998	23.668108928893446
14-15	23.978484354405918	24.29738903154324	24.455118359352436	27.269008254698406
16-17	23.81800456225895	27.149280650800446	25.261018744776546	23.771696042164056
18-19	23.802652183523236	26.099342754837362	27.02942678894723	23.06857827269217
20-21	24.921865077242234	24.978199675546847	26.74787403761044	23.35206120960048
22-23	24.716679247760016	24.725327381929173	26.48498473858676	24.073008631724047
24-25	24.114559034949632	25.228435916921416	26.639608095975987	24.017396952152968
26-27	23.769907110135215	25.453696928663007	26.83454898440119	23.94184697680059
28-29	23.781825891367824	25.466768253309784	26.993078575780643	23.758327279541753
30-31	24.136128231888623	25.227487545044603	26.791035714051294	23.845348509015484
32-33	24.009254777122322	25.185760114207888	26.569693886760255	24.235291221909534
34-35	24.071196329780236	25.40105697025708	26.674140657844685	23.853606042118
36-37	24.15770559912673	25.256220973862053	26.59481402677219	23.991259400239027
38-39	24.046287832664053	25.422335856741736	26.704843270245387	23.826533040348824
40-41	24.16687726253811	25.323245517589953	26.739221046896695	23.770656172975244
42-43	24.182821630037665	25.20312128726844	26.562858965758522	24.051198116935375
44-45	24.14297738099946	25.26107020198531	26.42543911829926	24.17051329871597
46-47	24.249794913392652	25.388056353319712	26.28515333782864	24.076995395459
48-49	24.188909008712603	25.592443114548715	26.111179028051158	24.10746884868753
50-51	24.195671926864094	25.812032687256174	25.997337272231828	23.9949581136479
52-53	24.341306256714688	25.623896748183476	26.02030844857257	24.014488546529268
54-55	24.182477970241013	25.648749058246985	25.927036972185487	24.241735999326515
56-57	24.133337402980754	25.798660101406423	25.833170055140442	24.23483244047238
58-59	23.995562233100582	25.74769578750179	26.05332146603182	24.20342051336581
60-61	24.111004539722366	25.886903233270182	26.04683142475736	23.955260802250088
62-63	23.94812394000087	25.87304543750118	26.01716019104805	24.161670431449902
64-65	23.955187237877805	25.689764163050267	26.070659840631016	24.284388758440915
66-67	23.945737113434205	25.80226053786977	25.945771904068437	24.306230444627587
68-69	23.968800004332238	25.72173210124901	25.994170841506193	24.315297052912552
70-71	23.83248008284421	25.607525562640674	26.163441710778855	24.39655264373626
72-73	23.965676945508115	25.606942468641908	26.032484929012252	24.39489565683773
74-75	23.884775826185727	25.71183552868978	25.95576402293469	24.4476246221898
76-77	23.86865098573292	25.53831764966486	26.00042661766033	24.59260474694189
78-79	23.764852715020186	25.60523398420655	26.065066755582215	24.564846545191045
80-81	23.687187983925924	25.74012924437446	26.014856818230676	24.55782595346894
82-83	23.774035822628548	25.589305426035523	26.0475697648259	24.589088986510024
84-85	23.739645541306587	25.53942291347115	25.94088482750609	24.780046717716175
86-87	23.68078940723082	25.579453070532317	26.04137798410599	24.698379538130872
88-89	23.544799954450262	25.488851554294516	26.031477558960738	24.934870932294483
90-91	23.50699473563219	25.503444245007152	26.080796879873862	24.908764139486795
92-93	23.474151902767893	25.519538984683965	26.123949636712013	24.88235947583613
94-95	23.46916121922673	25.39031804823173	26.16999425543195	24.970526477109594
96-97	23.50741431733639	25.244007095166133	26.200574636257873	25.0480039512396
98-99	23.34312692408288	25.350720047151004	26.041445495927285	25.26470753283883
100-101	23.215051340999437	25.360151626554465	26.23419319258991	25.190603839856184
102-103	23.094388784789107	25.254209477929567	26.264861565522153	25.38654017175917
104-105	23.060547457627674	25.397371302892186	26.1391967020356	25.402884537444542
106-107	23.03326982285104	25.35517083182105	26.104760792133014	25.506798553194898
108-109	22.926441963838162	25.36316645875231	26.14997037569136	25.56042120171816
110-111	22.825568692875166	25.458910153400478	26.129666011787815	25.58585514193654
112-113	22.73607669921916	25.368863305273138	26.253524888869713	25.641535106637992
114-115	22.623904686948055	25.491361942934038	26.197684737865647	25.687048632252257
116-117	22.5737399749762	25.42374911361743	26.15717019103206	25.845340720374317
118-119	22.60715958000788	25.32833417717199	26.1145971181734	25.94990912464673
120-121	22.37424995562159	25.399643594595577	26.21146501914752	26.01464143063531
122-123	22.255845007926894	25.412575042169305	26.182803717035053	26.14877623286874
124-125	22.195435232050713	25.488637324270226	26.172671306012134	26.143256137666924
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	32.0
1	47.5
2	108.5
3	163.0
4	147.5
5	124.0
6	141.0
7	391.0
8	670.5
9	778.5
10	729.5
11	604.5
12	580.0
13	617.0
14	729.0
15	840.0
16	949.5
17	1075.0
18	1232.5
19	1428.5
20	1669.5
21	1910.5
22	2188.5
23	2628.5
24	3126.5
25	3869.0
26	5197.5
27	6998.0
28	9199.0
29	12307.0
30	16500.5
31	21170.0
32	26631.0
33	31892.0
34	36710.5
35	42195.0
36	48899.5
37	55724.0
38	61846.5
39	68172.0
40	74635.5
41	80747.0
42	87260.5
43	95435.5
44	103565.5
45	110722.0
46	118286.0
47	124861.0
48	130192.5
49	134575.0
50	135566.5
51	134526.0
52	132027.0
53	130706.0
54	129424.0
55	123311.5
56	113330.5
57	101985.0
58	93185.5
59	84479.5
60	73496.5
61	62496.5
62	51190.5
63	38834.5
64	28494.0
65	21631.5
66	16249.5
67	12107.5
68	8914.5
69	6531.0
70	5006.0
71	3845.0
72	2821.0
73	2191.5
74	1666.5
75	1221.0
76	835.0
77	453.0
78	272.5
79	150.0
80	88.5
81	61.5
82	43.5
83	31.0
84	21.0
85	14.5
86	9.0
87	5.0
88	6.0
89	4.0
90	2.5
91	3.5
92	3.0
93	3.0
94	3.5
95	5.0
96	5.5
97	4.0
98	2.0
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07339828824823517
2	1.968662179210599E-4
3	6.890317627237098E-4
4	0.18459489033731385
5	0.06637672647571738
6	0.009646444678131937
7	0.01296035934646978
8	0.010827641985658297
9	0.018111692048737513
10-11	0.017685148576575216
12-13	0.018849940365941488
14-15	0.02503482071229479
16-17	0.026232423537981234
18-19	0.023574729596046926
20-21	0.025658230402378145
22-23	0.028053436053751043
24-25	0.025740257993178587
26-27	0.02491998208517417
28-29	0.025149659339415403
30-31	0.02542855314813691
32-33	0.022311504697720124
34-35	0.02441141102221143
36-37	0.02705269944598565
38-39	0.031170484504167824
40-41	0.027725325690549273
42-43	0.026478506310382562
44-45	0.02708551048230583
46-47	0.028594818153033955
48-49	0.0240340841045294
50-51	0.028857306443595368
52-53	0.026133990429020704
54-55	0.02987444856952084
56-57	0.026511317346702735
58-59	0.023853623404768428
60-61	0.0250184151941347
62-63	0.02879168437095501
64-65	0.032450114920654714
66-67	0.03143297279472924
68-69	0.027282376700226887
70-71	0.03518983645338946
72-73	0.038487345603567216
74-75	0.02478873793989346
76-77	0.017389849249693626
78-79	0.021950583298198184
80-81	0.024165328249810106
82-83	0.02344348545076622
84-85	0.01696330577753133
86-87	0.016618789896169477
88-89	0.017603120985774774
90-91	0.01871869622066078
92-93	0.02147482327155562
94-95	0.01734063269521336
96-97	0.02001473215530776
98-99	0.01719298303177257
100-101	0.017439065804173894
102-103	0.015257131888882143
104-105	0.017783581685535746
106-107	0.016044596760566382
108-109	0.013993906990555343
110-111	0.012205705511105716
112-113	0.010450315067976263
114-115	0.009236306724129728
116-117	0.008399625297965225
118-119	0.005709120319710738
120-121	0.0033467257046580186
122-123	0.007169211435958599
124-125	0.05602484451670164
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	3047755.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.6553927269816
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.19068865268184	41.84767306377206
2	13.426172742210726	14.944778335760667
3	4.581833596832271	7.6501124472413595
4	2.122321154119259	4.724744693011528
5	1.1660099231261452	3.2447370097571606
6	0.7446181963022396	2.486521088811469
7	0.5161762896966876	2.0109595883597757
8	0.3708620354401422	1.6512377783959105
9	0.2717334475933174	1.3611088568576475
>10	1.51413833450211	15.0425416073112
>50	0.0757197468241947	2.8446980457082818
>100	0.019076055585918784	1.6418177447970281
>500	2.9537503899698225E-4	0.11869112356918674
>1k	3.5445004633361324E-4	0.43037861664682386
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3585	0.1176275652078333	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3192	0.10473282793400389	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	3.281103632017665E-5
7	0.0	0.0	0.0	0.0	3.281103632017665E-5
8	0.0	0.0	0.0	0.0	3.281103632017665E-5
9	0.0	0.0	0.0	1.6405518160088328E-4	3.281103632017665E-5
10-11	0.0	0.0	0.0	2.1327173608114825E-4	3.281103632017665E-5
12-13	0.0	0.0	0.0	2.4608277240132487E-4	1.148386271206183E-4
14-15	0.0	0.0	0.0	2.952993268815899E-4	1.4764966344079495E-4
16-17	0.0	0.0	0.0	3.445158813618549E-4	1.6405518160088328E-4
18-19	0.0	0.0	0.0	4.1013795400220816E-4	2.296772542412366E-4
20-21	0.0	0.0	0.0	4.265434721622965E-4	2.4608277240132487E-4
22-23	0.0	0.0	0.0	5.905986537631798E-4	3.2811036320176657E-4
24-25	0.0	0.0	0.0	8.53086944324593E-4	4.265434721622965E-4
26-27	0.0	0.0	0.0	0.0012304138620066246	4.265434721622965E-4
28-29	0.0	0.0	0.0	0.0029201822324957227	4.265434721622965E-4
30-31	0.0	0.0	0.0	0.009630039159971848	4.4294899032238484E-4
32-33	0.0	0.0	0.0	0.024772332421733375	4.593545084824732E-4
34-35	0.0	0.0	0.0	0.04706743160129341	4.593545084824732E-4
36-37	0.0	0.0	0.0	0.07899256994082529	4.593545084824732E-4
38-39	0.0	0.0	0.0	0.12505926493435332	4.757600266425615E-4
40-41	0.0	0.0	0.0	0.17463674081414024	4.921655448026499E-4
42-43	0.0	0.0	0.0	0.22836481278842952	4.921655448026499E-4
44-45	0.0	0.0	0.0	0.29925305675817115	4.921655448026499E-4
46-47	0.0	0.0	0.0	0.37711364594595037	6.070041719232681E-4
48-49	0.0	0.0	0.0	0.4518243756469926	6.562207264035331E-4
50-51	0.0	0.0	0.0	0.5254031245949888	6.562207264035331E-4
52-53	0.0	1.6405518160088326E-5	0.0	0.605888596688382	7.218427990438864E-4
54-55	0.0	6.56220726403533E-5	0.0	0.7074059430630086	7.218427990438864E-4
56-57	0.0	6.56220726403533E-5	0.0	0.8225562750286686	7.218427990438864E-4
58-59	0.0	6.56220726403533E-5	0.0	0.9396588636553791	7.218427990438864E-4
60-61	0.0	6.56220726403533E-5	0.0	1.0597964731417058	7.218427990438864E-4
62-63	0.0	6.56220726403533E-5	0.0	1.1854299312116623	7.546538353640631E-4
64-65	0.0	6.56220726403533E-5	0.0	1.3363442927663147	7.546538353640631E-4
66-67	0.0	6.56220726403533E-5	0.0	1.5083233396385207	7.546538353640631E-4
68-69	0.0	6.56220726403533E-5	0.0	1.6895386932348564	8.53086944324593E-4
70-71	0.0	6.56220726403533E-5	0.0	1.8747569932622536	9.51520053285123E-4
72-73	0.0	6.56220726403533E-5	0.0	2.064618054928956	0.0011155752348860062
74-75	0.0	6.56220726403533E-5	0.0	2.2876838853516768	0.0011155752348860062
76-77	0.0	6.56220726403533E-5	0.0	2.5414936568064035	0.0011319807530460945
78-79	0.0	6.56220726403533E-5	0.0	2.8118237850483387	0.0011483862712061829
80-81	0.0	6.56220726403533E-5	0.0	3.0837944651062834	0.001197602825686448
82-83	0.0	6.56220726403533E-5	0.0	3.364033526316912	0.0012796304164868896
84-85	0.0	6.56220726403533E-5	0.0	3.6739993864336205	0.0012796304164868896
86-87	0.0	6.56220726403533E-5	0.0	4.025356368868232	0.0012796304164868896
88-89	0.0	6.56220726403533E-5	0.0	4.408015079952293	0.0012796304164868896
90-91	0.0	6.56220726403533E-5	0.0	4.792904941506125	0.0012796304164868896
92-93	0.0	6.56220726403533E-5	0.0	5.18379922270655	0.0013124414528070663
94-95	0.0	6.56220726403533E-5	0.0	5.606241315328824	0.0013124414528070663
96-97	0.0	6.56220726403533E-5	0.0	6.075242268489429	0.001361658007287331
98-99	0.0	6.56220726403533E-5	0.0	6.577234718669972	0.0013780635254474194
100-101	0.0	6.56220726403533E-5	0.0	7.094484300739397	0.001410874561767596
102-103	0.0	6.56220726403533E-5	0.0	7.604285777564142	0.001410874561767596
104-105	0.0	6.56220726403533E-5	0.0	8.14261645046928	0.001410874561767596
106-107	0.0	6.56220726403533E-5	0.0	8.718089216488858	0.001410874561767596
108-109	0.0	6.56220726403533E-5	0.0	9.33848029123076	0.001410874561767596
110-111	0.0	6.56220726403533E-5	0.0	9.972980111590335	0.001410874561767596
112-113	0.0	6.56220726403533E-5	0.0	10.602673115129004	0.001410874561767596
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5430	0.0	57.99869	1
GTATCAA	8335	0.0	43.22305	1
TCAACGC	10990	0.0	32.49956	4
ATCAACG	11300	0.0	31.712992	3
TATCAAC	11375	0.0	31.710443	2
CAACGCA	11395	0.0	31.342148	5
AACGCAG	11940	0.0	29.844046	6
ACGCAGA	13865	0.0	25.528912	7
GTACATG	13560	0.0	24.940634	1
TACATGG	13905	0.0	24.139915	2
CGCAGAG	14590	0.0	24.057262	8
ACATGGG	13790	0.0	23.30589	3
CATGGGG	9935	0.0	22.986837	4
GCAGAGT	15295	0.0	22.44421	9
GAGTACT	6895	0.0	21.52817	12-13
AGAGTAC	13875	0.0	21.48222	10-11
CAGAGTA	14980	0.0	21.267776	10-11
GTACTTT	7035	0.0	20.635302	14-15
AGTACTT	7415	0.0	19.577154	12-13
GTGGTAT	3080	0.0	18.203766	1
>>END_MODULE