FastQCFastQC Report
Mon 15 Aug 2016
SRR2921128_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921128_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2407346
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA32220.13384033703505852No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT30320.12594782802305943No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA95350.026.786081
CATGGGG65700.026.3540614
AACGCAG104150.023.879156
TCAACGC103550.023.8461324
GTACATG131400.023.741521
TATCAAC106800.023.6238482
CAACGCA105350.023.4951745
TACATGG132300.023.1634582
ATCAACG108750.022.8172253
ACATGGG135550.021.9056953
ACGCAGA114950.021.2215357
CGCAGAG117400.020.6270548
GGTATCA75400.020.5293221
GTGTAGC17700.020.5177231
TAGCCCT20200.019.1461724
GCAGAGT123000.018.9623379
GTAGCCC16750.018.8285583
GAGTACT30400.018.79096812-13
ATGGGGA31600.018.452665
ATGGGGC37250.018.0497885
Produced by FastQC (version 0.11.5)