##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921128_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2407346 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.591222034555898 31.0 30.0 34.0 25.0 34.0 2 29.594798171928755 31.0 30.0 34.0 25.0 34.0 3 29.652049601511376 31.0 30.0 34.0 25.0 34.0 4 33.044321007449696 35.0 35.0 37.0 26.0 37.0 5 33.164449979354856 37.0 35.0 37.0 27.0 37.0 6 33.14188197292786 37.0 35.0 37.0 26.0 37.0 7 33.03844731916393 37.0 35.0 37.0 26.0 37.0 8 32.98384611102849 37.0 35.0 37.0 26.0 37.0 9 34.31002689268597 38.0 35.0 39.0 26.0 39.0 10-11 34.28161572952122 38.0 34.5 39.0 25.5 39.0 12-13 34.298147420437274 38.0 35.0 39.0 26.0 39.0 14-15 35.36245226070536 39.0 35.0 41.0 25.0 41.0 16-17 35.30636809166609 39.0 35.0 41.0 25.0 41.0 18-19 35.39307893422882 39.5 35.0 41.0 25.5 41.0 20-21 35.427933915606644 40.0 35.0 41.0 25.0 41.0 22-23 35.421078856134514 39.0 35.0 41.0 25.0 41.0 24-25 35.35304999779841 39.0 35.0 41.0 25.0 41.0 26-27 35.30100679337328 39.0 35.0 41.0 25.0 41.0 28-29 35.16150150414606 39.0 34.5 41.0 25.0 41.0 30-31 35.05519376940415 39.0 34.0 41.0 24.0 41.0 32-33 34.949615053257816 39.0 34.0 41.0 24.0 41.0 34-35 34.84466545315879 39.0 34.0 41.0 24.0 41.0 36-37 34.710808292617685 39.0 34.0 41.0 24.0 41.0 38-39 34.55108592616101 39.0 34.0 41.0 23.0 41.0 40-41 34.43694321464385 39.0 34.0 41.0 22.0 41.0 42-43 34.35351191727321 39.0 33.5 41.0 21.5 41.0 44-45 34.197708389238606 38.5 33.0 41.0 20.5 41.0 46-47 33.96679517609849 38.0 33.0 40.0 18.5 41.0 48-49 33.825221011022094 38.0 33.0 40.0 17.5 41.0 50-51 33.67602725158743 38.0 33.0 40.0 16.0 41.0 52-53 33.47656527146493 38.0 33.0 40.0 14.5 41.0 54-55 33.239291319153956 38.0 32.0 40.0 13.0 41.0 56-57 32.98385483432793 38.0 32.0 40.0 9.0 41.0 58-59 32.765311052088066 37.0 32.0 40.0 8.0 41.0 60-61 32.467814348249064 37.0 31.0 40.0 7.5 41.0 62-63 32.202622722284204 36.5 31.0 40.0 6.5 41.0 64-65 31.89299357051292 36.0 31.0 39.0 2.0 41.0 66-67 31.617517589910214 35.5 31.0 39.0 2.0 41.0 68-69 31.25411303568328 35.0 30.0 39.0 2.0 40.0 70-71 30.934427373547468 35.0 30.0 38.0 2.0 40.0 72-73 30.57138608243269 35.0 30.0 37.5 2.0 40.0 74-75 30.49827195592158 35.0 30.0 37.0 2.0 39.0 76-77 30.231732164798913 35.0 30.0 36.5 2.0 39.0 78-79 29.952521573550293 35.0 30.0 36.0 2.0 39.0 80-81 29.594124608593862 34.0 29.0 36.0 2.0 37.5 82-83 29.32201312150393 34.0 29.0 35.0 2.0 37.0 84-85 29.107901606167125 34.0 29.0 35.0 2.0 37.0 86-87 28.88075851996348 34.0 29.0 35.0 2.0 36.0 88-89 28.705280836240405 34.0 29.0 35.0 2.0 36.0 90-91 28.529001232062196 34.0 29.0 35.0 2.0 36.0 92-93 28.68574396866923 34.0 29.0 35.0 2.0 35.0 94-95 28.650391759223645 34.0 29.0 35.0 2.0 35.0 96-97 28.575625398260158 34.0 29.0 35.0 2.0 35.0 98-99 28.457180230843427 34.0 29.0 35.0 2.0 35.0 100-101 28.34982840023827 34.0 29.0 35.0 2.0 35.0 102-103 27.045379642145335 32.5 26.0 34.5 2.0 35.0 104-105 28.0415995457238 34.0 28.5 35.0 2.0 35.0 106-107 28.1918259776534 34.0 29.0 35.0 2.0 35.0 108-109 28.124416058181914 34.0 29.0 35.0 2.0 35.0 110-111 28.094595459065708 34.0 29.0 35.0 2.0 35.0 112-113 28.00396972433543 34.0 29.0 35.0 2.0 35.0 114-115 27.85566137979335 34.0 29.0 35.0 2.0 35.0 116-117 27.680869721261505 34.0 28.5 35.0 2.0 35.0 118-119 27.554204505708775 34.0 28.0 35.0 2.0 35.0 120-121 27.357220358020825 34.0 27.0 35.0 2.0 35.0 122-123 27.19054801428627 34.0 27.0 35.0 2.0 35.0 124-125 26.129360507380326 33.0 23.5 34.5 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 92945.0 3 13228.0 4 8933.0 5 8783.0 6 11244.0 7 11902.0 8 11615.0 9 12492.0 10 13496.0 11 14498.0 12 14874.0 13 15530.0 14 16541.0 15 15026.0 16 12134.0 17 10866.0 18 10067.0 19 9521.0 20 9578.0 21 10349.0 22 12088.0 23 16046.0 24 22031.0 25 28703.0 26 33286.0 27 37216.0 28 43295.0 29 51464.0 30 61322.0 31 73848.0 32 91593.0 33 116749.0 34 154814.0 35 221589.0 36 367173.0 37 557112.0 38 195161.0 39 234.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.329847345314825 18.99591906633159 13.327004242209108 24.347229346144477 2 15.392332893248927 20.25288374376951 40.05111515283447 24.303668210147087 3 19.155587316359767 24.662509140464003 29.16169480821645 27.020208734959784 4 12.7107507444127 16.791050512042098 37.080248665208934 33.417950078336276 5 13.609137068490623 38.052008371990226 34.387974777676135 13.950879781843017 6 32.01640288176249 36.70807406970435 17.399251725493095 13.87627132304006 7 27.907852051136437 32.9354180851961 20.942666554222892 18.21406330944457 8 25.502575868797482 36.282548359075406 19.826898958062007 18.387976814065105 9 25.956202317625625 17.141207333816606 19.658358968180938 37.244231380376824 10-11 24.54629655979077 27.030648623149826 27.669922373604987 20.753132443454415 12-13 25.00162015842657 24.614547693309827 27.596594676242496 22.78723747202111 14-15 22.728816421553507 26.02725584388366 25.12901649188554 26.114911242677298 16-17 22.496309265959894 29.001764198913893 26.04348533251296 22.45844120261325 18-19 22.391526913962434 28.16666344715652 27.65702537617899 21.784784262702058 20-21 23.51729894068448 27.186432552037243 27.285060485428907 22.011208021849367 22-23 23.117131933541277 26.97572342748755 26.954537325653437 22.95260731331773 24-25 22.58907667331879 27.173619546271016 27.29346574434251 22.94383803606768 26-27 22.605959006980335 27.33853190562645 27.131512607572873 22.923996479820342 28-29 22.635023907384046 27.388500235656863 27.35279339072324 22.623682466235845 30-31 23.04478710634495 27.363685477668138 27.017266329608326 22.57426108637859 32-33 22.609478423347866 27.33508551982779 27.037114118103517 23.018321938720828 34-35 22.694254842778843 27.428327039280415 27.177638106877943 22.6997800110628 36-37 22.928575864685406 27.202063982887935 27.100451750702735 22.76890840172392 38-39 22.83399967141768 27.4278172974291 27.03700788202939 22.701175149123827 40-41 22.84760467873376 27.35859990246407 27.07824641364373 22.71554900515844 42-43 22.85236575565957 27.230080784940085 26.996979002561982 22.92057445683837 44-45 22.96221262132635 27.28620900150106 26.929647486591296 22.82193089058129 46-47 22.69587806700283 27.350139048331574 26.943742804308556 23.010240080357036 48-49 22.698482295314662 27.384069158365655 27.090959052858892 22.82648949346079 50-51 22.732564527723756 27.51675621941389 26.841626335216613 22.909052917645745 52-53 22.693754452722732 27.420484497774417 26.674151048817418 23.211610000685432 54-55 22.744143316681402 27.361590310184514 26.726856216599298 23.16741015653479 56-57 22.84324762181697 27.33207327711544 26.59940597349728 23.225273127570308 58-59 22.669817381368922 27.1439665731242 26.980255138102706 23.205960907404172 60-61 22.84236303816819 27.207050397966135 26.941829647230854 23.008756916634816 62-63 22.561377083202654 27.166802850174744 26.912138997736463 23.359681068886147 64-65 22.531702517570277 27.12152377296775 26.93353007141102 23.413243638050957 66-67 22.754045271146257 27.185098597318092 26.725084398938737 23.33577173259691 68-69 22.863154930068927 26.963601114776353 26.818563045138973 23.354680910015748 70-71 22.56088885694922 26.81506565505616 27.10750746733967 23.51653802065495 72-73 22.70479094338996 26.78508413462787 26.992016998279194 23.51810792370298 74-75 22.63034804480529 26.935909658310848 26.9384643823695 23.495277914514364 76-77 22.590746819468436 26.73893844644336 27.13998908321432 23.53032565087388 78-79 22.541802310680282 26.883186151703352 27.159258153075815 23.41575338454055 80-81 22.674013352474763 26.85777354628171 27.00339045853023 23.464822642713298 82-83 22.68980501552436 26.707529102075295 27.10024381626983 23.502422066130517 84-85 22.735946028295597 26.741224893663556 26.840068615280256 23.68276046276059 86-87 22.53299855172129 26.677385272253755 27.08405811309202 23.705558062932933 88-89 22.660918979207384 26.46637685915396 27.15869557541607 23.714008586222583 90-91 22.71686228115523 26.525316341330235 27.04011936883562 23.717702008678916 92-93 22.600132222575084 26.548116368328145 27.209146163270994 23.642605245825777 94-95 22.38640243856846 26.558168149231882 27.232363538782227 23.823065873417427 96-97 22.498111494223988 26.380426585259624 27.26146619496774 23.859995725548654 98-99 22.46530058151876 26.5003321124721 27.033934458984838 24.0004328470243 100-101 22.226223908469112 26.665437082795652 27.18587091820214 23.922468090533098 102-103 22.246045751145274 26.618296802537632 27.152695735110605 23.98296171120649 104-105 22.179713925788533 26.63996252974098 27.028290668680032 24.152032875790454 106-107 22.041978905861516 26.72038447845617 27.13812967631809 24.09950693936422 108-109 21.955279667216846 26.73434304366304 27.18929015206309 24.121087137057025 110-111 21.935921546829583 26.9095770380073 27.00585299668528 24.14864841847784 112-113 21.820448049064485 26.924318349208086 26.981415962656623 24.273817639070803 114-115 21.766610460355857 26.928493359305385 27.054967944124364 24.249928236214394 116-117 21.806291427523423 26.884072826244765 26.974921815658803 24.334713930573013 118-119 21.794946620089274 26.71105767223669 27.00495301932294 24.48904268835109 120-121 21.716903167809534 26.81691990892501 27.081890641051793 24.384286282213665 122-123 21.704440991092596 26.79399482329542 26.954110437195173 24.54745374841681 124-125 21.588936012234193 26.77493603426805 26.92819603644233 24.70793191705543 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1162.0 1 811.5 2 386.0 3 269.0 4 220.0 5 206.0 6 204.5 7 251.0 8 325.5 9 371.5 10 365.5 11 368.0 12 402.5 13 453.5 14 507.0 15 565.5 16 667.5 17 782.0 18 926.5 19 1136.0 20 1376.5 21 1699.0 22 2152.0 23 2706.5 24 3559.0 25 4740.0 26 6614.0 27 9447.5 28 12900.5 29 17082.5 30 22017.0 31 27210.0 32 33132.0 33 39548.0 34 46259.5 35 53476.0 36 60856.5 37 67067.5 38 70605.0 39 73664.5 40 76870.0 41 79660.0 42 81544.5 43 83264.5 44 86218.5 45 89053.0 46 91336.0 47 93618.5 48 96819.0 49 99327.0 50 103001.5 51 101832.0 52 93562.5 53 87677.0 54 82217.0 55 75959.0 56 69106.5 57 61793.5 58 56217.0 59 50113.0 60 42225.0 61 34553.0 62 27466.5 63 20377.5 64 14652.0 65 10695.5 66 7698.0 67 5737.0 68 4357.0 69 3246.0 70 2549.0 71 2043.5 72 1587.0 73 1271.0 74 1040.0 75 771.0 76 545.5 77 388.0 78 269.0 79 188.0 80 146.5 81 103.5 82 65.5 83 42.0 84 34.5 85 30.0 86 23.5 87 19.5 88 13.5 89 10.0 90 12.0 91 10.5 92 5.5 93 4.5 94 6.5 95 7.0 96 4.5 97 2.5 98 5.0 99 8.0 100 16.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06330623018045599 2 0.009637168898862066 3 0.019357416839955705 4 0.01723890126305068 5 0.011298749743493458 6 0.008640220392083231 7 0.009014076082125295 8 0.0081832856598096 9 0.005857072477325653 10-11 0.009948715307230453 12-13 0.00722787667414655 14-15 0.007996357814788567 16-17 0.012648784179756463 18-19 0.006127079364578253 20-21 0.013292646757051127 22-23 0.0044654985199468625 24-25 0.0039047149848837684 26-27 0.0034270104920522434 28-29 0.010156412912809377 30-31 0.006085539843462469 32-33 0.0028246874358733645 34-35 0.007456344040283366 36-37 0.006459395533504532 38-39 0.0014331134784945747 40-41 0.0011631065912419735 42-43 0.0011838763517998659 44-45 0.001973127252999777 46-47 0.003717787139862737 48-49 0.002471601506389194 50-51 0.004382419477715293 52-53 0.0046108868438521095 54-55 0.0048808937311047105 56-57 0.0019108179713260994 58-59 0.002554680548620763 60-61 0.004403189238273186 62-63 0.002284673661368162 64-65 0.003510089534283813 66-67 0.0012046461123577583 68-69 0.0019523574924418842 70-71 0.0012254158729156507 72-73 0.0018069691685366374 74-75 0.0017031203657471756 76-77 0.00182773892909453 78-79 1.2461856334735428E-4 80-81 8.307904223156954E-5 82-83 2.0769760557892385E-5 84-85 4.3616497171574005E-4 86-87 2.2846736613681625E-4 88-89 4.153952111578477E-5 90-91 7.269416195262334E-4 92-93 0.0017238901263050678 94-95 0.0013292646757051127 96-97 8.100206617578029E-4 98-99 0.0012046461123577583 100-101 0.001142336830684081 102-103 0.0025754503091786557 104-105 0.004299340435483724 106-107 0.005690914392862513 108-109 0.010509498842293546 110-111 0.008432522786504307 112-113 0.00270006887252601 114-115 0.005940151519557222 116-117 0.00226390390081027 118-119 0.0011007973095682965 120-121 0.004112412590462692 122-123 9.554089856630497E-4 124-125 0.0812720730630329 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2407346.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.91345947168717 #Duplication Level Percentage of deduplicated Percentage of total 1 68.07055990203767 31.934254531792977 2 14.276292235694715 13.395005144104525 3 6.386945952378451 8.989011902542885 4 3.4561033967973427 6.4855106654245 5 2.036771138719145 4.7775990134701365 6 1.3586715961468645 3.8243990916701067 7 0.9079875861015099 2.9817787174957773 8 0.6625168066452167 2.4864764286289587 9 0.5128388612655734 2.165314062013981 >10 2.2209333374864455 17.441002808867896 >50 0.08173480961458848 2.5945468767340927 >100 0.027130220576635285 2.0259733893864706 >500 7.12544252215041E-4 0.24193454131223985 >1k 8.016122837411085E-4 0.657192826555468 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 3222 0.13384033703505852 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 3032 0.12594782802305943 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 1.246185633473543E-4 0.0 0.0 4.153952111578477E-5 0.0 6 1.6615808446313908E-4 0.0 0.0 4.153952111578477E-5 0.0 7 1.6615808446313908E-4 0.0 0.0 4.153952111578477E-5 0.0 8 1.6615808446313908E-4 0.0 0.0 4.153952111578477E-5 0.0 9 1.6615808446313908E-4 0.0 0.0 4.153952111578477E-5 0.0 10-11 7.477113800841258E-4 0.0 0.0 1.246185633473543E-4 0.0 12-13 9.969485067788345E-4 0.0 0.0 1.4538832390524668E-4 0.0 14-15 0.0011007973095682965 0.0 0.0 1.6615808446313908E-4 0.0 16-17 0.0012669553940314355 0.0 0.0 2.0769760557892385E-4 0.0 18-19 0.0013292646757051127 0.0 0.0 2.70006887252601E-4 2.0769760557892385E-5 20-21 0.0013708041968208975 0.0 0.0 3.3231616892627817E-4 4.153952111578477E-5 22-23 0.0013708041968208975 0.0 0.0 6.438625772946639E-4 4.153952111578477E-5 24-25 0.0018692784502103146 0.0 0.0 0.0015992715629577136 4.153952111578477E-5 26-27 0.0021392853374629156 0.0 0.0 0.0031154640836838577 4.153952111578477E-5 28-29 0.002160055098020808 0.0 0.0 0.0066463233785255624 4.153952111578477E-5 30-31 0.002326213182483947 0.0 0.0 0.0169065850941244 4.153952111578477E-5 32-33 0.002492371266947086 0.0 0.0 0.03734402948309051 4.153952111578477E-5 34-35 0.002741608393641795 0.0 0.0 0.06733556372868711 4.153952111578477E-5 36-37 0.0029908455203365033 0.0 0.0 0.11741145643376565 4.153952111578477E-5 38-39 0.0029908455203365033 0.0 0.0 0.19463342618800955 4.153952111578477E-5 40-41 0.0031570036047996425 0.0 0.0 0.28502342413595716 4.153952111578477E-5 42-43 0.0031570036047996425 0.0 0.0 0.36378235617148513 4.153952111578477E-5 44-45 0.003281622168146997 0.0 0.0 0.4536115705843697 4.153952111578477E-5 46-47 0.0034062407314943513 0.0 0.0 0.5640028479495678 4.153952111578477E-5 48-49 0.0034477802526101356 0.0 0.0 0.6776549777223548 4.153952111578477E-5 50-51 0.0034477802526101356 0.0 0.0 0.795523368888394 4.153952111578477E-5 52-53 0.0034893197737259204 0.0 0.0 0.9157179732369174 4.153952111578477E-5 54-55 0.003530859294841705 0.0 0.0 1.0507006471026599 4.153952111578477E-5 56-57 0.003530859294841705 0.0 0.0 1.2094439270466315 4.153952111578477E-5 58-59 0.00357239881595749 0.0 0.0 1.3820198675221593 4.153952111578477E-5 60-61 0.0036970173793048444 0.0 0.0 1.5556135262650237 4.153952111578477E-5 62-63 0.003738556900420629 0.0 0.0 1.734295776344572 4.153952111578477E-5 64-65 0.003738556900420629 0.0 0.0 1.9321692851796128 4.153952111578477E-5 66-67 0.003738556900420629 0.0 0.0 2.158310438133945 4.153952111578477E-5 68-69 0.003946254505999553 0.0 0.0 2.4201755792478523 4.153952111578477E-5 70-71 0.003987794027115337 0.0 0.0 2.7047420686515355 4.153952111578477E-5 72-73 0.0040501033087890145 0.0 0.0 2.985570001154799 4.153952111578477E-5 74-75 0.004070873069346908 0.0 0.0 3.2800852058657126 4.153952111578477E-5 76-77 0.004133182351020585 0.0 0.0 3.6163476292979904 4.153952111578477E-5 78-79 0.004320110196041616 0.0 0.0 3.9943572714516318 4.153952111578477E-5 80-81 0.004320110196041616 0.0 0.0 4.384267986404946 4.153952111578477E-5 82-83 0.004403189238273186 0.0 0.0 4.792809176578689 4.153952111578477E-5 84-85 0.004507038041062648 0.0 0.0 5.223283233901567 4.153952111578477E-5 86-87 0.00452780780162054 0.0 0.0 5.679532564076789 4.153952111578477E-5 88-89 0.00452780780162054 0.0 0.0 6.182451546225595 6.230928167367716E-5 90-91 0.004777044928315249 0.0 0.0 6.730212441418891 8.307904223156954E-5 92-93 0.004777044928315249 0.0 0.0 7.285346601610238 8.307904223156954E-5 94-95 0.0048601239705468174 0.0 0.0 7.851530274418384 8.307904223156954E-5 96-97 0.005067821576125742 0.0 0.0 8.458277289596095 1.246185633473543E-4 98-99 0.005317058702820451 2.0769760557892385E-5 0.0 9.113770932803178 1.246185633473543E-4 100-101 0.005379367984494128 4.153952111578477E-5 0.0 9.809059437239185 1.246185633473543E-4 102-103 0.005441677266167805 4.153952111578477E-5 0.0 10.521462224374893 1.246185633473543E-4 104-105 0.005794763195651975 4.153952111578477E-5 0.0 11.234176557918971 1.246185633473543E-4 106-107 0.005877842237883545 4.153952111578477E-5 0.0 11.96350669990936 1.246185633473543E-4 108-109 0.005898611998441437 4.153952111578477E-5 0.0 12.735684857930684 1.246185633473543E-4 110-111 0.005940151519557222 4.153952111578477E-5 0.0 13.543358536745444 1.246185633473543E-4 112-113 0.005940151519557222 4.153952111578477E-5 0.0 14.379341399200612 1.246185633473543E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9535 0.0 26.78608 1 CATGGGG 6570 0.0 26.354061 4 AACGCAG 10415 0.0 23.87915 6 TCAACGC 10355 0.0 23.846132 4 GTACATG 13140 0.0 23.74152 1 TATCAAC 10680 0.0 23.623848 2 CAACGCA 10535 0.0 23.495174 5 TACATGG 13230 0.0 23.163458 2 ATCAACG 10875 0.0 22.817225 3 ACATGGG 13555 0.0 21.905695 3 ACGCAGA 11495 0.0 21.221535 7 CGCAGAG 11740 0.0 20.627054 8 GGTATCA 7540 0.0 20.529322 1 GTGTAGC 1770 0.0 20.517723 1 TAGCCCT 2020 0.0 19.146172 4 GCAGAGT 12300 0.0 18.962337 9 GTAGCCC 1675 0.0 18.828558 3 GAGTACT 3040 0.0 18.790968 12-13 ATGGGGA 3160 0.0 18.45266 5 ATGGGGC 3725 0.0 18.049788 5 >>END_MODULE