FastQCFastQC Report
Mon 15 Aug 2016
SRR2921128_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921128_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2407346
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41640.17297056592612778No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36830.1529900562694353No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT30810.12798326455773287No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA29050.12067230884135476No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24090.10006870636792552No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA112300.031.8692191
GGTATCA82800.030.1844751
TCAACGC131650.026.8634244
TATCAAC132300.026.6385122
CAACGCA133800.026.4257015
ATCAACG133650.026.4157073
AACGCAG135100.026.156746
GAGTACT69750.023.62662112-13
CATGGGG60150.023.4985074
ACGCAGA149750.023.359477
ACGTATA1552.0757063E-423.0835112
CGCAGAG152800.022.8148578
CCGAACG1602.615407E-422.3087239
AGTACTT76000.021.56622112-13
GTACATG125500.020.7700561
GTACTTT80050.020.73544914-15
GCAGAGT160300.020.7082869
TACATGG126600.020.0187782
TAGGACG2107.0232796E-519.879544
TACCGTA3902.764864E-1019.830397
Produced by FastQC (version 0.11.5)