##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921128_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2407346
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.42053697308156	31.0	30.0	34.0	27.0	34.0
2	30.49252704015127	31.0	30.0	34.0	27.0	34.0
3	30.584461892889514	31.0	30.0	34.0	27.0	34.0
4	34.10025148026084	37.0	35.0	37.0	30.0	37.0
5	33.72735576855176	37.0	35.0	37.0	28.0	37.0
6	33.57891387444929	37.0	35.0	37.0	28.0	37.0
7	33.388112053689	37.0	35.0	37.0	27.0	37.0
8	33.36709887153737	37.0	35.0	37.0	27.0	37.0
9	34.73306371414828	39.0	35.0	39.0	27.0	39.0
10-11	34.69046597373207	38.5	35.0	39.0	27.0	39.0
12-13	34.554518129093196	38.0	35.0	39.0	27.0	39.0
14-15	35.58766521306036	39.0	35.0	41.0	27.0	41.0
16-17	35.530403190899854	39.0	35.0	41.0	27.0	41.0
18-19	35.59477158663524	39.0	35.0	41.0	27.0	41.0
20-21	35.60681223222586	39.0	35.0	41.0	27.0	41.0
22-23	35.58750632439209	39.0	35.0	41.0	27.0	41.0
24-25	35.49612498577271	39.0	35.0	41.0	26.0	41.0
26-27	35.38289468983686	39.0	35.0	41.0	25.5	41.0
28-29	35.279449651192635	39.0	35.0	41.0	25.0	41.0
30-31	35.178304448135	39.0	34.5	41.0	25.0	41.0
32-33	35.014037034975445	39.0	34.0	41.0	25.0	41.0
34-35	34.8797057008008	39.0	34.0	41.0	24.0	41.0
36-37	34.72842478812767	39.0	34.0	41.0	24.0	41.0
38-39	34.55958553527412	39.0	34.0	41.0	23.0	41.0
40-41	34.39795255854372	39.0	33.5	41.0	22.0	41.0
42-43	34.257208560796826	38.5	33.0	41.0	20.5	41.0
44-45	34.06520500169066	38.0	33.0	41.0	18.5	41.0
46-47	33.80689232042258	38.0	33.0	41.0	16.0	41.0
48-49	33.57390981603808	38.0	33.0	40.0	14.0	41.0
50-51	33.357970146376964	38.0	33.0	40.0	10.0	41.0
52-53	33.18535370486835	38.0	32.5	40.0	8.5	41.0
54-55	32.989472223768416	38.0	32.0	40.0	8.0	41.0
56-57	32.72919555394198	38.0	32.0	40.0	7.0	41.0
58-59	32.512982138836705	37.0	31.5	40.0	4.0	41.0
60-61	32.25109103552211	37.0	31.0	40.0	2.0	41.0
62-63	31.962483166109067	36.0	31.0	40.0	2.0	41.0
64-65	31.68897117406472	36.0	31.0	39.0	2.0	41.0
66-67	31.36658149680187	35.5	30.5	39.0	2.0	41.0
68-69	31.010799029304472	35.0	30.0	39.0	2.0	40.0
70-71	30.6521436885267	35.0	30.0	38.0	2.0	40.0
72-73	30.46019703856446	35.0	30.0	37.0	2.0	40.0
74-75	30.260803183256584	35.0	30.0	37.0	2.0	39.0
76-77	30.018049337320022	35.0	30.0	36.5	2.0	39.0
78-79	29.68366948498471	35.0	29.0	36.0	2.0	39.0
80-81	29.38300123870852	34.5	29.0	36.0	2.0	37.5
82-83	29.14460447314179	34.0	29.0	35.0	2.0	37.0
84-85	28.968899775935824	34.0	29.0	35.0	2.0	36.5
86-87	28.74410595734888	34.0	29.0	35.0	2.0	36.0
88-89	28.58297000098864	34.0	29.0	35.0	2.0	36.0
90-91	28.411918976333276	34.0	29.0	35.0	2.0	36.0
92-93	28.2807473458323	34.0	29.0	35.0	2.0	35.0
94-95	28.173081061052294	34.0	29.0	35.0	2.0	35.0
96-97	28.099293578903904	34.0	29.0	35.0	2.0	35.0
98-99	28.056479417582683	34.0	29.0	35.0	2.0	35.0
100-101	27.907218364123814	34.0	28.0	35.0	2.0	35.0
102-103	26.5609216539708	32.0	25.0	34.0	2.0	35.0
104-105	27.63115273001887	34.0	27.0	35.0	2.0	35.0
106-107	27.900845786189436	34.0	28.5	35.0	2.0	35.0
108-109	28.09492092121365	34.0	29.0	35.0	2.0	35.0
110-111	28.07770652826806	34.0	29.0	35.0	2.0	35.0
112-113	28.000793820248525	34.0	29.0	35.0	2.0	35.0
114-115	27.88005691745183	34.0	29.0	35.0	2.0	35.0
116-117	27.774805117336683	34.0	29.0	35.0	2.0	35.0
118-119	27.629435901611153	34.0	29.0	35.0	2.0	35.0
120-121	27.453724142686596	34.0	28.5	35.0	2.0	35.0
122-123	27.27200929986799	34.0	27.0	35.0	2.0	35.0
124-125	26.482138836710636	33.5	25.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	65841.0
3	22441.0
4	11020.0
5	9014.0
6	10289.0
7	12088.0
8	13731.0
9	14904.0
10	15974.0
11	17361.0
12	18796.0
13	20461.0
14	23210.0
15	19657.0
16	13448.0
17	11957.0
18	11099.0
19	10494.0
20	10263.0
21	10501.0
22	11699.0
23	14283.0
24	19451.0
25	25647.0
26	31576.0
27	37480.0
28	44560.0
29	52658.0
30	62210.0
31	74508.0
32	91877.0
33	116112.0
34	151460.0
35	213846.0
36	355788.0
37	556160.0
38	205130.0
39	352.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.028091473332886	19.58372009788659	14.089758197503285	24.298430231277234
2	15.70566861944361	20.566218080446436	39.18834099531392	24.53977230479604
3	18.861822119776235	24.77823023678842	29.975628692095295	26.384318951340045
4	13.037001033678614	17.321690439055406	36.676490220002094	32.96481830726388
5	13.385296233839702	37.73126739792146	34.78220930169726	14.10122706654158
6	31.920937752895995	37.00046030187162	17.058878757816405	14.019723187415977
7	27.579324770931873	32.74273120249602	21.101604255858348	18.576339770713762
8	25.768302263352517	35.911283859825936	19.698781891684288	18.62163198513726
9	26.001275496955635	16.182358672236752	19.86700763013081	37.949358200676805
10-11	24.691259029981357	26.47846566919238	27.819686008527523	21.010589292298743
12-13	25.525182414729784	23.802930601384865	28.03417954711456	22.63770743677079
14-15	22.9425572859654	25.344997620204595	25.712780405028507	25.9996646888015
16-17	22.34662301062558	28.479791248778145	26.65731081141442	22.516274929181854
18-19	22.227545198788246	27.254932674964692	28.775505794727096	21.742016331519967
20-21	23.25131149690042	26.27470892154453	28.560913268457405	21.913066313097644
22-23	22.808243312083913	26.16508230929747	28.29995504131462	22.72671933730399
24-25	22.329431875172176	26.453282431139314	28.626583958911496	22.59070173477702
26-27	22.38110285164519	26.526825674716374	28.527982013795878	22.564089459842563
28-29	22.342441614302672	26.527115220730696	28.732656309793743	22.39778685517289
30-31	22.698837932814712	26.43962018571053	28.537220533073782	22.32432134840098
32-33	22.409932251960136	26.4682290964442	28.35807345523729	22.763765196358378
34-35	22.416437605990986	26.682107963458602	28.534668855233996	22.366785575316417
36-37	22.715626231591184	26.421291286156713	28.39050755736034	22.472574924891767
38-39	22.67447006285738	26.634044708648325	28.27136172435415	22.420123504140143
40-41	22.623274714932577	26.516375859980656	28.326202103115616	22.534147321971147
42-43	22.799017152463737	26.32090134107955	28.154048699027562	22.726032807429153
44-45	22.85151506526526	26.46880337361222	28.031309541345053	22.648372019777465
46-47	22.74051630145729	26.599065259015	27.77409816774811	22.8863202717796
48-49	22.818661301768852	26.710518224531278	27.683196801005174	22.7876236726947
50-51	22.786751496337303	26.965671656985414	27.38347641397616	22.86410043270113
52-53	22.754928545132866	26.90888413732697	27.25394254525087	23.08224477228929
54-55	22.71553418471355	26.905196621626676	27.27326745344681	23.106001740212964
56-57	22.842023079860404	26.84701001833641	27.086904113414	23.224062788389183
58-59	22.554551228076516	26.81658243487124	27.484599053953012	23.144267283099232
60-61	22.861413037268303	26.9095930629339	27.344437364740905	22.884556535056895
62-63	22.56603301619646	26.887748339280083	27.348204153763113	23.198014490760343
64-65	22.47082389121853	26.86005419792944	27.395343415957164	23.27377849489487
66-67	22.647133985576954	26.92430912940228	27.193884229181425	23.23467265583934
68-69	22.722079782704306	26.694823084717108	27.277203460625856	23.30589367195273
70-71	22.488862903542493	26.496597006961814	27.512830437927192	23.5017096515685
72-73	22.614876671854255	26.458650631548984	27.3716383704187	23.554834326178064
74-75	22.522158117434127	26.559180865873767	27.337641657073803	23.581019359618306
76-77	22.427432356721347	26.393493398900127	27.516094809547265	23.662979434831264
78-79	22.34527591206363	26.557160654867225	27.489453357007836	23.608110076061305
80-81	22.38124520877783	26.531456386114854	27.339103228634524	23.748195176472787
82-83	22.356207697453435	26.461086850841586	27.371747196450013	23.81095825525497
84-85	22.420127170005177	26.39210730823489	27.1351475766592	24.05261794510073
86-87	22.207307058964542	26.39219442559276	27.325970892996583	24.074527622446116
88-89	22.238771678576043	26.224268538264766	27.4076446855264	24.12931509763279
90-91	22.231801204043393	26.28626567894669	27.37763254656424	24.104300570445677
92-93	22.167207077245752	26.250658547489376	27.408684184722365	24.17345019054251
94-95	21.96845229798251	26.250095297883007	27.461494981255143	24.319957422879344
96-97	22.084748910505887	26.016047524337814	27.44257953649811	24.45662402865819
98-99	22.04845516646544	26.15971575362101	27.201293267844544	24.590535812069003
100-101	21.812670420576065	26.240478986984574	27.298057719576903	24.64879287286246
102-103	21.758180991798028	26.134096557431675	27.25915690271053	24.84856554805977
104-105	21.718234945610675	26.100998543358156	27.226509884474094	24.954256626557083
106-107	21.53801124278682	26.12355681874127	27.37056264412597	24.96786929434593
108-109	21.404939535917524	26.164667122973235	27.4253403863511	25.00505295475814
110-111	21.280986628329547	26.28670624596371	27.27710264755876	25.155204478147986
112-113	21.16485006124591	26.290064037651955	27.180679485829312	25.36440641527282
114-115	21.099851795230236	26.26832437215495	27.22786457386715	25.403959258747665
116-117	21.07235022964898	26.20428622704341	27.22869183167494	25.494671711632666
118-119	20.970331245940613	26.10019111181547	27.289172054430207	25.64030558781371
120-121	20.84706168739793	26.22891573021689	27.271110959718136	25.652911622667045
122-123	20.73821096804171	26.203184674876397	27.121581249796183	25.93702310728571
124-125	20.47005110109455	26.247862128872846	27.166268705722953	26.11581806430966
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	145.0
1	125.5
2	162.0
3	239.5
4	201.5
5	136.5
6	216.0
7	501.0
8	809.5
9	909.5
10	777.0
11	617.5
12	587.5
13	660.5
14	765.0
15	891.0
16	1016.5
17	1162.0
18	1394.0
19	1587.5
20	1863.5
21	2260.5
22	2710.5
23	3389.5
24	4344.5
25	5541.5
26	7525.5
27	10454.5
28	13692.0
29	17938.5
30	22929.0
31	27958.5
32	33547.0
33	39525.0
34	45958.0
35	52848.0
36	59889.0
37	65756.0
38	69513.5
39	72745.0
40	76415.0
41	79336.0
42	80813.0
43	82822.0
44	85834.0
45	88933.5
46	91849.0
47	93985.0
48	96637.0
49	99350.0
50	104202.0
51	102816.0
52	93645.5
53	87636.0
54	82035.0
55	75755.5
56	68828.5
57	61286.5
58	55727.5
59	49558.5
60	41450.5
61	33949.0
62	27183.0
63	20106.0
64	14237.0
65	10327.0
66	7339.5
67	5369.5
68	4005.5
69	2980.0
70	2339.0
71	1889.0
72	1462.0
73	1137.0
74	866.0
75	665.0
76	489.0
77	313.5
78	188.5
79	109.5
80	82.0
81	53.5
82	37.0
83	19.5
84	10.0
85	10.5
86	9.5
87	5.5
88	3.0
89	3.0
90	4.0
91	2.5
92	1.5
93	1.0
94	1.0
95	2.0
96	3.0
97	3.5
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07082488350241303
2	2.0769760557892385E-4
3	2.9077664781049337E-4
4	0.17770607133332725
5	0.06343084874380334
6	0.009554089856630496
7	0.012918791067009064
8	0.010509498842293546
9	0.018318928812061083
10-11	0.018007382403692696
12-13	0.019440495882187273
14-15	0.02602450997903916
16-17	0.027146077049165347
18-19	0.024466777937197228
20-21	0.02697991896470221
22-23	0.028745348612123058
24-25	0.02654375399298647
26-27	0.025442956683418172
28-29	0.026377595908523326
30-31	0.0267306818380075
32-33	0.023282901585397364
34-35	0.024778324345565615
36-37	0.02710453752804956
38-39	0.032691603118122615
40-41	0.028184565077059964
42-43	0.027644551302554766
44-45	0.02772763034478633
46-47	0.028662269569891492
48-49	0.02450831745831301
50-51	0.029887685442807142
52-53	0.02708376776749167
54-55	0.03090540371014387
56-57	0.027374544415302165
58-59	0.024695245303334043
60-61	0.025858351894576016
62-63	0.029451520471091397
64-65	0.03308622856872257
66-67	0.032442365991427906
68-69	0.027893788429249475
70-71	0.03578629744124858
72-73	0.03991947979226916
74-75	0.025318338120070817
76-77	0.01813200096704005
78-79	0.02232749259973431
80-81	0.024965252190586644
82-83	0.02392676416269203
84-85	0.017051973418029647
86-87	0.016595038685756014
88-89	0.017405059347513817
90-91	0.018942021628797854
92-93	0.022182104275829066
94-95	0.01742582910807171
96-97	0.02056206295231346
98-99	0.017446598868629603
100-101	0.017986612643134805
102-103	0.015120385686145656
104-105	0.018277389290945297
106-107	0.015805787784556106
108-109	0.014061127897693143
110-111	0.011610296151861842
112-113	0.010239491955040946
114-115	0.0084532925470622
116-117	0.008058667096462245
118-119	0.004839354209988926
120-121	0.002970075759778611
122-123	0.00670863266019924
124-125	0.05528910260510953
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2407346.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.77751874542799
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.38646600138294	31.989491953132642
2	14.165267277757168	13.252321112385673
3	6.326811064460844	8.878575694997952
4	3.3770493233829932	6.318799521151315
5	2.01161565757769	4.704919456546843
6	1.3211670571743832	3.7080550069689986
7	0.9297519174188812	3.044404142196153
8	0.6785351598548477	2.5392152927633695
9	0.4922523248380062	2.072370811733131
>10	2.1962087187138026	17.317158643937915
>50	0.08431984078977166	2.672494782084612
>100	0.028771679799246334	2.2420566982381636
>500	8.919884247665923E-4	0.3053018634890025
>1k	8.919884247659894E-4	0.9548350203743221
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4164	0.17297056592612778	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3683	0.1529900562694353	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	3081	0.12798326455773287	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	2905	0.12067230884135476	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2409	0.10006870636792552	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	8.307904223156954E-5	0.0
3	0.0	0.0	0.0	8.307904223156954E-5	0.0
4	0.0	0.0	0.0	8.307904223156954E-5	0.0
5	8.307904223156954E-5	0.0	0.0	8.307904223156954E-5	0.0
6	1.6615808446313908E-4	0.0	0.0	8.307904223156954E-5	0.0
7	1.6615808446313908E-4	0.0	0.0	8.307904223156954E-5	0.0
8	1.6615808446313908E-4	0.0	0.0	8.307904223156954E-5	0.0
9	1.6615808446313908E-4	0.0	0.0	8.307904223156954E-5	0.0
10-11	3.738556900420629E-4	0.0	0.0	1.246185633473543E-4	0.0
12-13	5.815532956209867E-4	0.0	0.0	1.4538832390524668E-4	0.0
14-15	7.477113800841258E-4	0.0	0.0	1.6615808446313908E-4	0.0
16-17	0.001038488027894619	0.0	0.0	2.492371266947086E-4	0.0
18-19	0.0011631065912419735	0.0	0.0	3.5308592948417056E-4	0.0
20-21	0.0012046461123577583	0.0	0.0	4.153952111578477E-4	0.0
22-23	0.001246185633473543	0.0	0.0	6.438625772946639E-4	0.0
24-25	0.001786199407978745	0.0	0.0	0.0015992715629577136	0.0
26-27	0.0020146667741155616	0.0	0.0	0.0030531548020101807	0.0
28-29	0.0020769760557892386	0.0	0.0	0.006750172181315024	0.0
30-31	0.0021600550980208078	0.0	0.0	0.016657347967429695	0.0
32-33	0.0023677527035997317	0.0	0.0	0.036617087863564275	0.0
34-35	0.002533910788062871	0.0	0.0	0.06586091072907675	0.0
36-37	0.00270006887252601	0.0	0.0	0.11512678277239749	0.0
38-39	0.002824687435873364	0.0	0.0	0.1904171647947574	0.0
40-41	0.0029077664781049337	0.0	0.0	0.2787717262080316	0.0
42-43	0.0029077664781049337	0.0	0.0	0.35636755165231754	0.0
44-45	0.0031154640836838577	0.0	0.0	0.44418209929108654	0.0
46-47	0.0032608524075891047	0.0	0.0	0.5521848541921269	0.0
48-49	0.003281622168146997	0.0	0.0	0.6635730800641038	0.0
50-51	0.003281622168146997	0.0	0.0	0.7798214299066275	0.0
52-53	0.0034270104920522434	0.0	0.0	0.898603690537214	0.0
54-55	0.003530859294841705	0.0	0.0	1.0322986392483673	0.0
56-57	0.003530859294841705	0.0	0.0	1.1885080084042758	0.0
58-59	0.003530859294841705	0.0	0.0	1.3586123473734146	0.0
60-61	0.003593168576515382	0.0	0.0	1.5296720953282161	0.0
62-63	0.003613938337073275	0.0	0.0	1.7060904415069542	0.0
64-65	0.003613938337073275	0.0	0.0	1.9002253934415743	0.0
66-67	0.0036554778581890596	0.0	0.0	2.1220464361998648	0.0
68-69	0.0036970173793048444	0.0	0.0	2.3789683743009937	0.0
70-71	0.003717787139862737	0.0	0.0	2.6572416262556358	0.0
72-73	0.003738556900420629	0.0	0.0	2.934559469224615	0.0
74-75	0.003738556900420629	0.0	0.0	3.2255230448801293	0.0
76-77	0.003780096421536414	0.0	0.0	3.556198402722334	0.0
78-79	0.003987794027115338	0.0	0.0	3.9290779140181757	0.0
80-81	0.003987794027115338	0.0	0.0	4.31583162536669	0.0
82-83	0.004029333548231123	0.0	0.0	4.719325763724866	0.0
84-85	0.004070873069346908	0.0	0.0	5.144420453063249	0.0
86-87	0.004070873069346908	0.0	0.0	5.5945219341133345	0.0
88-89	0.004112412590462692	0.0	0.0	6.0882606820955525	0.0
90-91	0.004153952111578477	0.0	0.0	6.626612875756123	0.0
92-93	0.004153952111578477	0.0	0.0	7.1723383344147456	0.0
94-95	0.0041954916326942615	0.0	0.0	7.728303285028408	0.0
96-97	0.004320110196041616	0.0	0.0	8.325101584898889	0.0
98-99	0.004423958998831077	0.0	0.0	8.96937955740471	0.0
100-101	0.004486268280504755	0.0	0.0	9.654428569885674	0.0
102-103	0.004527807801620539	0.0	0.0	10.357090339319733	4.153952111578477E-5
104-105	0.005192440139473096	0.0	0.0	11.06143445935898	4.153952111578477E-5
106-107	0.005275519181704665	0.0	0.0	11.784284436055309	4.153952111578477E-5
108-109	0.005275519181704665	0.0	0.0	12.5500655078248	4.153952111578477E-5
110-111	0.005275519181704665	0.0	0.0	13.354769110879783	4.153952111578477E-5
112-113	0.005296288942262558	0.0	0.0	14.184500275407025	4.153952111578477E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	11230	0.0	31.869219	1
GGTATCA	8280	0.0	30.184475	1
TCAACGC	13165	0.0	26.863424	4
TATCAAC	13230	0.0	26.638512	2
CAACGCA	13380	0.0	26.425701	5
ATCAACG	13365	0.0	26.415707	3
AACGCAG	13510	0.0	26.15674	6
GAGTACT	6975	0.0	23.626621	12-13
CATGGGG	6015	0.0	23.498507	4
ACGCAGA	14975	0.0	23.35947	7
ACGTATA	155	2.0757063E-4	23.083511	2
CGCAGAG	15280	0.0	22.814857	8
CCGAACG	160	2.615407E-4	22.308723	9
AGTACTT	7600	0.0	21.566221	12-13
GTACATG	12550	0.0	20.770056	1
GTACTTT	8005	0.0	20.735449	14-15
GCAGAGT	16030	0.0	20.708286	9
TACATGG	12660	0.0	20.018778	2
TAGGACG	210	7.0232796E-5	19.87954	4
TACCGTA	390	2.764864E-10	19.83039	7
>>END_MODULE