FastQCFastQC Report
Mon 15 Aug 2016
SRR2921127_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921127_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1454777
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT24340.16731086620148655No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA22890.15734370284930269No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG42100.021.7660144
GTACATG94150.021.0587711
TACATGG92550.020.6387712
GTATCAA67800.020.0223121
GTATAGG3301.7211278E-819.846681
GTGTAGC10550.019.7526191
GGTATCA56050.019.4394761
CGTAGGA1908.318214E-418.7911282
ACATGGG97650.018.7077433
CGTTACG1000.001174768117.85034424-25
GAGTACT18600.017.43447712-13
TATCAAC76300.017.3914412
AACGCAG76900.017.0992226
GTACCGT2100.001628851316.9997466
TCAACGC78400.016.8491214
ATAGTAC4602.4829205E-916.8166983
ATCAACG79550.016.6809183
CAACGCA78900.016.6669275
CCTAGAC5458.0035534E-1116.377593
GTTTAGG10050.015.99583151
Produced by FastQC (version 0.11.5)