##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921127_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1454777
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.29152852980216	31.0	28.0	34.0	16.0	34.0
2	28.321197681844023	31.0	28.0	34.0	16.0	34.0
3	28.352478077396054	31.0	28.0	34.0	16.0	34.0
4	31.616269022674953	35.0	32.0	37.0	17.0	37.0
5	31.747003836326805	35.0	32.0	37.0	17.0	37.0
6	31.70321636924422	35.0	32.0	37.0	17.0	37.0
7	31.56381837216288	35.0	32.0	37.0	17.0	37.0
8	31.492485789918316	35.0	32.0	37.0	16.0	37.0
9	32.66499057931215	37.0	32.0	39.0	13.0	39.0
10-11	32.627529167700615	37.0	32.0	39.0	13.0	39.0
12-13	32.638816464654035	37.0	32.0	39.0	12.0	39.0
14-15	33.57691900545582	38.0	32.0	41.0	11.0	41.0
16-17	33.504887346995446	38.0	32.0	41.0	10.5	41.0
18-19	33.58590869940891	38.5	32.0	41.0	11.0	41.0
20-21	33.60475626161261	39.0	32.0	41.0	11.0	41.0
22-23	33.5904282924462	39.0	32.0	41.0	11.0	41.0
24-25	33.50925846366832	38.5	32.0	41.0	10.5	41.0
26-27	33.43065191434839	38.5	32.0	41.0	10.0	41.0
28-29	33.27243419438169	38.0	32.0	41.0	9.5	41.0
30-31	33.16609281010079	38.0	32.0	41.0	8.5	41.0
32-33	33.02444154671128	38.0	31.5	40.0	8.0	41.0
34-35	32.889195732404346	38.0	31.0	40.0	6.5	41.0
36-37	32.73082575542506	38.0	31.0	40.0	2.0	41.0
38-39	32.562881802503064	38.0	31.0	40.0	2.0	41.0
40-41	32.430645727833195	38.0	31.0	40.0	2.0	41.0
42-43	32.31911351361755	38.0	30.5	40.0	2.0	41.0
44-45	32.137376037702	38.0	30.0	40.0	2.0	41.0
46-47	31.877302844353466	38.0	30.0	40.0	2.0	41.0
48-49	31.71641461199895	37.5	30.0	40.0	2.0	41.0
50-51	31.551370416221868	37.0	30.0	40.0	2.0	41.0
52-53	31.33370819032745	37.0	30.0	40.0	2.0	41.0
54-55	31.074429276789502	37.0	29.0	40.0	2.0	41.0
56-57	30.79955209630067	36.0	29.0	40.0	2.0	41.0
58-59	30.569775642589896	36.0	29.0	40.0	2.0	41.0
60-61	30.255092704930036	35.5	28.0	39.5	2.0	41.0
62-63	29.97462703905822	35.0	28.0	39.0	2.0	41.0
64-65	29.662508068246886	35.0	28.0	39.0	2.0	40.5
66-67	29.385024646389105	35.0	28.0	38.5	2.0	40.0
68-69	29.04375687820195	34.5	27.0	38.0	2.0	40.0
70-71	28.7267210026004	34.0	26.0	37.0	2.0	40.0
72-73	28.36701226373527	34.0	26.0	37.0	2.0	39.0
74-75	28.30603075247959	34.0	26.0	36.5	2.0	39.0
76-77	28.051460808082613	34.0	26.0	36.0	2.0	39.0
78-79	27.78695978833869	34.0	26.0	36.0	2.0	38.5
80-81	27.445443528458313	34.0	26.0	35.0	2.0	37.0
82-83	27.192123603823816	34.0	26.0	35.0	2.0	37.0
84-85	26.99143511342288	34.0	25.0	35.0	2.0	36.0
86-87	26.76955712112578	34.0	25.0	35.0	2.0	36.0
88-89	26.600123249130277	33.0	25.0	35.0	2.0	36.0
90-91	26.437604526329466	33.0	24.5	35.0	2.0	35.0
92-93	26.584090551335358	34.0	25.0	35.0	2.0	35.0
94-95	26.56111005329339	34.0	25.0	35.0	2.0	35.0
96-97	26.499466241217725	34.0	25.0	35.0	2.0	35.0
98-99	26.385315068907467	34.0	24.5	35.0	2.0	35.0
100-101	26.26217695220642	34.0	24.0	35.0	2.0	35.0
102-103	25.012858671810182	31.5	21.0	34.0	2.0	35.0
104-105	25.966288991371187	33.0	23.5	35.0	2.0	35.0
106-107	26.120432891089152	34.0	24.0	35.0	2.0	35.0
108-109	26.069554646519705	34.0	23.0	35.0	2.0	35.0
110-111	26.046256917726907	34.0	23.0	35.0	2.0	35.0
112-113	25.956048590265034	34.0	23.0	35.0	2.0	35.0
114-115	25.829113328022096	34.0	21.5	35.0	2.0	35.0
116-117	25.656366233450214	34.0	19.5	35.0	2.0	35.0
118-119	25.521107015027045	34.0	19.0	35.0	2.0	35.0
120-121	25.32826268218428	33.0	17.5	35.0	2.0	35.0
122-123	25.159659865395177	33.5	4.5	35.0	2.0	35.0
124-125	24.136062778006526	32.0	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	94938.0
3	12277.0
4	8197.0
5	8341.0
6	10278.0
7	10308.0
8	9970.0
9	10748.0
10	11176.0
11	11875.0
12	12005.0
13	12068.0
14	12294.0
15	11091.0
16	9101.0
17	8176.0
18	7349.0
19	6977.0
20	6937.0
21	7575.0
22	8834.0
23	11643.0
24	15621.0
25	19956.0
26	22566.0
27	25643.0
28	29461.0
29	34181.0
30	39348.0
31	46643.0
32	57186.0
33	70783.0
34	92519.0
35	128397.0
36	205811.0
37	278591.0
38	85833.0
39	80.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.180177267521245	19.72890519936577	13.80375377905877	24.287163754054212
2	15.738004919437001	20.928574669436657	38.82581095777781	24.50760945334853
3	18.937638149323305	25.046081285944606	29.252764705275712	26.763515859456376
4	13.256056980997165	17.515606523114155	35.56685366993923	33.66148282594945
5	14.039093075838476	38.08457265107096	33.48739302269785	14.388941250392712
6	31.9930897989291	36.26741900690264	17.239274305147383	14.500216889020878
7	27.395961656914075	33.45148268731577	20.958288420080073	18.194267235690088
8	25.648034020110416	35.68585018310007	19.906452945652926	18.759662851136582
9	26.020284706315838	17.359344796011804	19.474706673302663	37.1456638243697
10-11	24.60381732286929	27.406416193236506	27.03859120382337	20.951175280070835
12-13	24.90132692641496	25.24270843537485	27.08060721362639	22.7753574245838
14-15	22.918216277825977	26.521827003587077	24.69749069545861	25.862466023128334
16-17	22.500653100508732	29.021002337412348	25.83751546816994	22.64082909390898
18-19	22.516401052169556	28.1826221541979	27.304954910210533	21.996021883422003
20-21	23.89125554410899	27.17525176433545	26.840242007253618	22.093250684301942
22-23	23.394227067751867	26.890088814342278	26.518058457985045	23.19762565992081
24-25	22.88180129336493	27.150352384373072	26.763717728173393	23.204128594088605
26-27	22.786410313077532	27.130705203798218	26.8905584554718	23.19232602765245
28-29	22.767129785581215	27.35633803785155	27.099434220385938	22.777097956181297
30-31	23.210562532610062	27.318519628594153	26.672952088040812	22.797965750754972
32-33	22.760833909611215	27.333169271717654	26.6531499142801	23.25284690439103
34-35	22.872984539702195	27.393933227764673	26.825862924123584	22.90721930840955
36-37	23.06877964980831	27.141402732817806	26.754722143358478	23.035095474015407
38-39	22.899281257990985	27.316595681498857	26.797920487446763	22.986202573063398
40-41	22.968179784116632	27.385725686656354	26.79783085538784	22.848263673839178
42-43	23.068571212532607	27.24570635119762	26.69761095434658	22.988111481923198
44-45	23.081269578092744	27.285803549885856	26.554784976947943	23.078141895073454
46-47	22.864171171517974	27.329370164044413	26.647902563228314	23.1585561012093
48-49	22.858597214694157	27.324648399732876	26.78283392616489	23.03392045940808
50-51	22.73560765025632	27.4828704443631	26.617963219789342	23.163558685591237
52-53	22.846111754993064	27.355954899850005	26.36022926780311	23.437704077353825
54-55	22.867648600464214	27.359217220973598	26.304267950859085	23.4688662277031
56-57	23.012777792480566	27.28371634861098	26.294577661255314	23.408928197653136
58-59	22.851834106552303	27.108777438733078	26.55376455327787	23.48562390143675
60-61	22.997856273022208	27.08915422632491	26.537222723849673	23.375766776803204
62-63	22.824257492951542	27.028414838145785	26.51526842575411	23.632059243148568
64-65	22.695614156892777	27.056985301699427	26.64429590177119	23.603104639636605
66-67	22.840561877691588	27.20040693857771	26.412238402766082	23.546792780964623
68-69	23.040477606194855	26.919859357761272	26.497107760413265	23.542555275630605
70-71	22.727141497080282	26.749509370929324	26.735108419572924	23.78824071241747
72-73	22.931463043771156	26.75930814134132	26.751265511277555	23.55796330360997
74-75	22.794999396801295	26.837977390531464	26.70998240584515	23.657040806822092
76-77	22.691643289176746	26.671854281191166	26.968366320741683	23.668136108890412
78-79	22.90446976336547	26.71688061727759	26.88993143269578	23.488718186661167
80-81	22.7686224801928	26.63626790140599	26.952021413635386	23.643088204765828
82-83	22.776652366651383	26.653260259132498	26.871747353718128	23.698340020497987
84-85	23.049099497137178	26.53782847535058	26.674860398942652	23.73821162856959
86-87	22.871720492379392	26.34734984336039	26.919363680575486	23.861565983684734
88-89	22.939014020705578	26.40469982684631	26.832119287010997	23.824166865437107
90-91	22.895746950716543	26.447997206427416	26.89239796311164	23.7638578797444
92-93	22.739392781234617	26.464739297920488	27.027682840653195	23.7681850801917
94-95	22.519147461921605	26.57943361182104	27.05968398954606	23.841734936711298
96-97	22.665229094948472	26.488072324934286	27.05960675516646	23.78709182495078
98-99	22.585140957571127	26.53014509569937	26.857414673445312	24.02729927328419
100-101	22.371219484699214	26.827446688874197	26.903335390943674	23.89799843548292
102-103	22.18951323528199	26.767591362902714	27.022824571678395	24.020070830136902
104-105	22.246251184071777	26.870214734596072	26.872689427424824	24.010844653907334
106-107	22.009377129935565	26.90970259010476	27.12510642159139	23.955813858368284
108-109	21.944003706779448	27.049384311633457	26.97462313492347	24.031988846663623
110-111	21.850234830861286	27.15242145265161	26.88827110590487	24.10907261058224
112-113	21.77838590456511	27.26146003359362	26.840835186756678	24.119318875084595
114-115	21.810155724221087	27.235618383886017	26.868838451158222	24.085387440734674
116-117	21.84104982878519	26.9693483732445	26.9980818029746	24.19151999499571
118-119	21.85760027055935	26.92873208300453	26.91113472700727	24.302532919428856
120-121	21.86696903840357	26.919516897220536	26.941136155614664	24.272377908761232
122-123	21.74538575567198	26.907297175516398	26.9558618371465	24.39145523166512
124-125	21.64615058610493	27.000008255968034	26.891407876468705	24.462433281458335
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	900.0
1	605.5
2	263.0
3	189.5
4	151.5
5	138.5
6	133.0
7	161.0
8	205.5
9	232.5
10	252.5
11	279.0
12	317.0
13	358.0
14	390.5
15	455.0
16	551.0
17	645.0
18	802.5
19	998.0
20	1207.5
21	1480.0
22	1815.5
23	2287.5
24	2930.0
25	3749.5
26	5087.0
27	6904.5
28	8819.0
29	11286.5
30	13697.0
31	16071.5
32	19047.5
33	22356.0
34	25724.5
35	28846.0
36	32625.5
37	36242.0
38	38466.5
39	40517.0
40	43545.5
41	45705.0
42	46653.5
43	48698.0
44	51366.5
45	53612.0
46	55427.0
47	56656.5
48	57975.0
49	59835.5
50	62798.0
51	61928.0
52	56036.0
53	52260.5
54	50576.0
55	47330.5
56	43361.0
57	39928.0
58	37096.5
59	33684.5
60	28357.0
61	23039.0
62	18248.0
63	13417.0
64	9758.5
65	7473.0
66	5691.5
67	4360.5
68	3278.5
69	2406.0
70	1900.0
71	1509.0
72	1090.5
73	807.5
74	615.0
75	445.5
76	315.0
77	218.0
78	154.0
79	97.5
80	64.0
81	49.5
82	42.0
83	35.0
84	25.0
85	22.0
86	14.0
87	8.5
88	10.0
89	12.0
90	11.0
91	8.0
92	9.0
93	7.5
94	5.0
95	7.5
96	9.0
97	7.0
98	6.0
99	8.5
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07162609802052136
2	0.009554729006576265
3	0.019384414243557604
4	0.0176659378035259
5	0.010035902409785143
6	0.0079737306817471
7	0.008317425969753439
8	0.007423818220936954
9	0.005842819896107788
10-11	0.009967163352183873
12-13	0.006839536231326176
14-15	0.007630035393740759
16-17	0.012166813195424453
18-19	0.004708625445686864
20-21	0.013232268588244108
22-23	0.003918126283272282
24-25	0.0030588880632564305
26-27	0.002852670890452626
28-29	0.01010464146738641
30-31	0.006049037068911593
32-33	0.002680823246449456
34-35	0.007939361152946465
36-37	0.006049037068911593
38-39	8.936077488164853E-4
40-41	6.87390576012681E-4
42-43	8.248686912152172E-4
44-45	0.0017872154976329706
46-47	0.0033682138224621367
48-49	0.0024402365448450173
50-51	0.003746278639269111
52-53	0.004055604398474818
54-55	0.004296191100079256
56-57	0.0018215850264336046
58-59	0.0024746060736456515
60-61	0.004639886388085597
62-63	0.0018215850264336046
64-65	0.003505691937664673
66-67	8.248686912152172E-4
68-69	0.0015122592672278982
70-71	8.248686912152172E-4
72-73	0.001993432670436775
74-75	0.0022340193720412132
76-77	0.0020621717280380433
78-79	1.3747811520253622E-4
80-81	2.062171728038043E-4
82-83	0.0
84-85	4.4680387440824266E-4
86-87	3.0932575920570644E-4
88-89	0.0
90-91	6.186515184114129E-4
92-93	8.936077488164853E-4
94-95	0.0010310858640190216
96-97	6.186515184114129E-4
98-99	8.936077488164853E-4
100-101	0.0010310858640190216
102-103	0.001993432670436775
104-105	0.003505691937664673
106-107	0.004639886388085597
108-109	0.009967163352183873
110-111	0.007767513508943296
112-113	0.0025089756024462857
114-115	0.0061865151841141285
116-117	0.0017528459688323365
118-119	8.936077488164853E-4
120-121	0.0038150176968703795
122-123	0.0010654553928196556
124-125	0.08819221090242697
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1454777.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.38234504918553
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.04217779011418	41.18289271306174
2	13.519048706663215	15.244713378316618
3	5.523448536510873	9.342749437409246
4	2.7780590101188167	6.265339267020717
5	1.548503814009669	4.365413817573649
6	1.0006909061101696	3.385277997353143
7	0.624623691294347	2.465242394191768
8	0.4278904025158196	1.9300371454305465
9	0.3088728874422444	1.5673479944496183
>10	1.174955864933228	10.549427643758714
>50	0.03734811420423953	1.3989476542489783
>100	0.012536707976557654	1.2754983963809188
>500	0.0013519499448047569	0.5326819039335515
>1k	4.916181617471843E-4	0.4944302568708066
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	2434	0.16731086620148655	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	2289	0.15734370284930269	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	3.436952880063405E-5	0.0
16-17	0.0	0.0	0.0	1.7184764400317025E-4	0.0
18-19	0.0	0.0	0.0	2.062171728038043E-4	0.0
20-21	0.0	0.0	0.0	3.0932575920570644E-4	0.0
22-23	0.0	0.0	0.0	6.530210472120469E-4	0.0
24-25	0.0	0.0	0.0	0.0018903240840348727	0.0
26-27	0.0	0.0	0.0	0.003505691937664673	0.0
28-29	0.0	0.0	0.0	0.007801883037743929	0.0
30-31	0.0	0.0	0.0	0.019315675185956338	0.0
32-33	0.0	0.0	0.0	0.04519593037283377	0.0
34-35	0.0	0.0	0.0	0.0817651090167084	0.0
36-37	0.0	0.0	0.0	0.14314908745464083	0.0
38-39	0.0	0.0	0.0	0.22697636819938727	0.0
40-41	0.0	0.0	0.0	0.3211832466419252	0.0
42-43	0.0	0.0	0.0	0.4037732243498488	0.0
44-45	0.0	0.0	0.0	0.49883934101240257	0.0
46-47	0.0	0.0	0.0	0.614320957782533	0.0
48-49	0.0	0.0	0.0	0.7304212260710747	0.0
50-51	6.87390576012681E-5	0.0	0.0	0.843015802421952	0.0
52-53	6.87390576012681E-5	0.0	0.0	0.9604564823337185	0.0
54-55	6.87390576012681E-5	0.0	0.0	1.095185035232204	0.0
56-57	6.87390576012681E-5	0.0	0.0	1.2568592987103866	0.0
58-59	6.87390576012681E-5	0.0	0.0	1.4265416623991167	0.0
60-61	6.87390576012681E-5	0.0	0.0	1.5867724056676726	0.0
62-63	6.87390576012681E-5	0.0	0.0	1.7517805134395168	0.0
64-65	6.87390576012681E-5	0.0	0.0	1.944765417655077	0.0
66-67	6.87390576012681E-5	0.0	0.0	2.172875980304885	0.0
68-69	6.87390576012681E-5	0.0	0.0	2.4196491970934377	0.0
70-71	6.87390576012681E-5	0.0	0.0	2.6849819594343325	0.0
72-73	6.87390576012681E-5	0.0	0.0	2.9482525500471892	0.0
74-75	6.87390576012681E-5	0.0	0.0	3.2335883781500536	0.0
76-77	6.87390576012681E-5	0.0	0.0	3.567110285631406	0.0
78-79	6.87390576012681E-5	0.0	0.0	3.935276678143798	0.0
80-81	6.87390576012681E-5	0.0	0.0	4.309457738196301	0.0
82-83	6.87390576012681E-5	0.0	0.0	4.698795760449883	0.0
84-85	6.87390576012681E-5	0.0	0.0	5.118000903231216	0.0
86-87	6.87390576012681E-5	0.0	0.0	5.578312002458109	0.0
88-89	6.87390576012681E-5	3.436952880063405E-5	0.0	6.091449067451575	0.0
90-91	6.87390576012681E-5	6.87390576012681E-5	0.0	6.613144145116399	0.0
92-93	6.87390576012681E-5	6.87390576012681E-5	0.0	7.140819520792533	0.0
94-95	6.87390576012681E-5	6.87390576012681E-5	0.0	7.699874276263648	0.0
96-97	6.87390576012681E-5	6.87390576012681E-5	0.0	8.29298923477619	0.0
98-99	6.87390576012681E-5	6.87390576012681E-5	0.0	8.941954677589761	0.0
100-101	6.87390576012681E-5	6.87390576012681E-5	0.0	9.6270424951728	0.0
102-103	6.87390576012681E-5	6.87390576012681E-5	0.0	10.302678692335665	0.0
104-105	6.87390576012681E-5	6.87390576012681E-5	0.0	10.996633848349266	0.0
106-107	6.87390576012681E-5	6.87390576012681E-5	0.0	11.719665625728204	0.0
108-109	6.87390576012681E-5	6.87390576012681E-5	0.0	12.489818027092813	0.0
110-111	6.87390576012681E-5	6.87390576012681E-5	0.0	13.286194378932304	0.0
112-113	6.87390576012681E-5	6.87390576012681E-5	0.0	14.094875022082423	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGGGG	4210	0.0	21.766014	4
GTACATG	9415	0.0	21.058771	1
TACATGG	9255	0.0	20.638771	2
GTATCAA	6780	0.0	20.022312	1
GTATAGG	330	1.7211278E-8	19.84668	1
GTGTAGC	1055	0.0	19.752619	1
GGTATCA	5605	0.0	19.439476	1
CGTAGGA	190	8.318214E-4	18.791128	2
ACATGGG	9765	0.0	18.707743	3
CGTTACG	100	0.0011747681	17.850344	24-25
GAGTACT	1860	0.0	17.434477	12-13
TATCAAC	7630	0.0	17.391441	2
AACGCAG	7690	0.0	17.099222	6
GTACCGT	210	0.0016288513	16.999746	6
TCAACGC	7840	0.0	16.849121	4
ATAGTAC	460	2.4829205E-9	16.816698	3
ATCAACG	7955	0.0	16.680918	3
CAACGCA	7890	0.0	16.666927	5
CCTAGAC	545	8.0035534E-11	16.37759	3
GTTTAGG	1005	0.0	15.9958315	1
>>END_MODULE