FastQCFastQC Report
Mon 15 Aug 2016
SRR2921127_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921127_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1454777
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32510.22347067626172257No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32350.22237085134010232No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT23990.16490499918544216No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA23600.16222417593899272No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20510.14098380714020087No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT15330.10537697530274398No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA89900.030.4508881
GCGTTAG800.005300846429.8206421
GGTATCA67700.029.4242061
TATCAAC106950.025.193882
TCAACGC108050.024.8856534
GAGTACT50850.024.86298412-13
ATCAACG108750.024.6697963
CAACGCA110850.024.1096045
AACGCAG111850.024.038816
CGCGTGA650.002053372822.88372824-25
AGTACTT56800.022.7298512-13
GTACTTT56750.022.33129514-15
ACGCAGA122200.021.7114647
CATGGGG41750.021.5634254
CGCAGAG123900.021.415048
GTCCTAG4152.910383E-1120.119951
TAGGGCG1500.004651617319.8735754
GCAGAGT128250.019.7159089
TACTTTT65450.019.54469914-15
TCTATAC5800.019.5302543
Produced by FastQC (version 0.11.5)