##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921127_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1454777
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.74090324496469	31.0	31.0	34.0	27.0	34.0
2	30.80209337926019	31.0	31.0	34.0	27.0	34.0
3	30.879557485442785	31.0	31.0	34.0	27.0	34.0
4	34.4146525549964	37.0	35.0	37.0	30.0	37.0
5	34.100776957568065	37.0	35.0	37.0	30.0	37.0
6	33.96951629012557	37.0	35.0	37.0	29.0	37.0
7	33.80013087916567	37.0	35.0	37.0	28.0	37.0
8	33.78250274784383	37.0	35.0	37.0	28.0	37.0
9	35.191091143178646	39.0	35.0	39.0	28.0	39.0
10-11	35.164604609503726	39.0	35.0	39.0	28.0	39.0
12-13	35.06092445783787	39.0	35.0	39.0	27.0	39.0
14-15	36.14045554748253	40.0	36.0	41.0	27.0	41.0
16-17	36.105016095250335	40.0	36.0	41.0	27.0	41.0
18-19	36.1820485201512	40.0	36.0	41.0	27.0	41.0
20-21	36.20145493089319	40.0	36.0	41.0	27.0	41.0
22-23	36.17653358555985	40.0	36.0	41.0	27.0	41.0
24-25	36.09133839756883	40.0	36.0	41.0	27.0	41.0
26-27	35.976804348707745	40.0	35.5	41.0	27.0	41.0
28-29	35.85727915687421	39.5	35.0	41.0	27.0	41.0
30-31	35.732128360566605	39.0	35.0	41.0	27.0	41.0
32-33	35.55539715021615	39.0	35.0	41.0	26.5	41.0
34-35	35.40603714521195	39.0	35.0	41.0	26.0	41.0
36-37	35.25486552234466	39.0	35.0	41.0	25.0	41.0
38-39	35.075434241811635	39.0	34.5	41.0	25.0	41.0
40-41	34.91057289192777	39.0	34.0	41.0	24.0	41.0
42-43	34.7529903208533	39.0	34.0	41.0	24.0	41.0
44-45	34.52946430965021	39.0	34.0	41.0	23.0	41.0
46-47	34.21991068046855	38.5	33.0	41.0	20.5	41.0
48-49	33.944874712756665	38.0	33.0	41.0	18.0	41.0
50-51	33.71754536949649	38.0	33.0	40.5	15.5	41.0
52-53	33.540634406510414	38.0	33.0	40.0	14.5	41.0
54-55	33.33687946674989	38.0	33.0	40.0	12.5	41.0
56-57	33.06095710889023	38.0	32.0	40.0	8.5	41.0
58-59	32.84030507768544	37.0	32.0	40.0	8.0	41.0
60-61	32.56367642600894	37.0	32.0	40.0	7.0	41.0
62-63	32.25932874935471	36.5	31.0	40.0	6.0	41.0
64-65	31.968805528269968	36.0	31.0	39.0	2.0	41.0
66-67	31.631477539169232	35.5	31.0	39.0	2.0	41.0
68-69	31.27223622589579	35.0	31.0	39.0	2.0	40.5
70-71	30.90239225668264	35.0	30.0	38.0	2.0	40.0
72-73	30.700119674699284	35.0	30.0	37.0	2.0	40.0
74-75	30.509173914627464	35.0	30.0	37.0	2.0	39.0
76-77	30.266505794358864	35.0	30.0	36.5	2.0	39.0
78-79	29.941481409178174	35.0	30.0	36.0	2.0	39.0
80-81	29.650904571628505	35.0	30.0	36.0	2.0	37.0
82-83	29.42199560482466	34.5	29.5	35.0	2.0	37.0
84-85	29.24400028320492	34.0	29.0	35.0	2.0	36.5
86-87	29.033216087414083	34.0	29.0	35.0	2.0	36.0
88-89	28.878100561116927	34.0	29.0	35.0	2.0	36.0
90-91	28.719394793841253	34.0	29.0	35.0	2.0	36.0
92-93	28.59028634629225	34.0	29.0	35.0	2.0	35.0
94-95	28.492382337636627	34.0	29.0	35.0	2.0	35.0
96-97	28.421697621009955	34.0	29.0	35.0	2.0	35.0
98-99	28.37305270842198	34.0	29.0	35.0	2.0	35.0
100-101	28.236132066976587	34.0	29.0	35.0	2.0	35.0
102-103	26.884301855198423	32.0	25.5	34.0	2.0	35.0
104-105	27.95032331415743	34.0	28.0	35.0	2.0	35.0
106-107	28.215047735838553	34.0	29.0	35.0	2.0	35.0
108-109	28.407044172405804	34.0	29.0	35.0	2.0	35.0
110-111	28.39209823911156	34.0	29.0	35.0	2.0	35.0
112-113	28.318828933919082	34.0	29.0	35.0	2.0	35.0
114-115	28.20408385615115	34.0	29.0	35.0	2.0	35.0
116-117	28.101856504467698	34.0	29.0	35.0	2.0	35.0
118-119	27.962720403195817	34.0	29.0	35.0	2.0	35.0
120-121	27.802464226475948	34.0	29.0	35.0	2.0	35.0
122-123	27.61551254934605	34.0	29.0	35.0	2.0	35.0
124-125	26.83589443605446	33.5	26.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	30388.0
3	10467.0
4	5276.0
5	4570.0
6	5371.0
7	6753.0
8	7765.0
9	8507.0
10	9434.0
11	10381.0
12	11625.0
13	13406.0
14	16017.0
15	13252.0
16	8256.0
17	7276.0
18	6936.0
19	6768.0
20	6534.0
21	6730.0
22	7229.0
23	8237.0
24	11052.0
25	14851.0
26	18664.0
27	21787.0
28	26105.0
29	31018.0
30	36466.0
31	44036.0
32	54436.0
33	67935.0
34	88934.0
35	126391.0
36	218388.0
37	345729.0
38	137496.0
39	311.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.573777303556454	20.04986203323998	14.573362474554747	23.802998188648818
2	16.348851196920485	21.1013386949872	38.29382550566239	24.25598460242993
3	18.778488160679586	24.8760114134802	30.39674285506104	25.94875757077918
4	13.471445494366236	17.601724565415026	35.60139397779553	33.32543596242321
5	13.874026451088325	37.28694051463092	34.082845039767435	14.756187994513317
6	31.716884511445876	36.2738540205097	17.117629306166727	14.891632161877702
7	27.180092163871024	33.02554752805443	21.13134829707208	18.663012011002465
8	25.917027070318753	35.01377336608418	19.92879235043678	19.140407213160284
9	26.168041817605552	15.98211627719908	19.797292140910976	38.05254976428439
10-11	24.97606589690237	26.349204494008138	27.371779380679946	21.302950228409546
12-13	25.799911240335526	24.03602776094293	27.667473708129208	22.496587290592334
14-15	23.371523299018975	25.358739985533514	25.636277877551915	25.633458837895596
16-17	22.36724402648828	28.14978076553636	26.74433555490006	22.7386396530753
18-19	22.323128738372137	26.71716308799379	29.104495827049355	21.855212346584718
20-21	23.577323815878994	25.769803706801625	28.820690321411764	21.832182155907617
22-23	23.106573819890777	25.654176128110674	28.50049935796833	22.738750694030223
24-25	22.530785148046146	26.0185596136399	28.799373209950947	22.651282028363006
26-27	22.38430526434124	26.034095882249602	28.938389306644634	22.643209546764524
28-29	22.34340949383821	26.0517950105421	29.210127980038592	22.394667515581098
30-31	22.806446378501622	25.932207002484876	28.821512405820137	22.439834213193365
32-33	22.443599911306833	26.053205587669588	28.713897497511947	22.78929700351163
34-35	22.439001583445805	26.259348208877338	28.857835719044594	22.44381448863226
36-37	22.76495351119805	25.92732654580208	28.73975725092952	22.567962692070346
38-39	22.694108166164483	26.05984385808331	28.677462563208323	22.568585412543886
40-41	22.79000481991995	26.10742989629265	28.606053021869787	22.496512261917616
42-43	22.942736669124763	25.896508987522154	28.499168888407585	22.661585454945502
44-45	22.94726282228027	26.006265285901804	28.20997921426769	22.83649267755024
46-47	22.97244706277108	26.090957972007008	27.964010385295566	22.972584579926345
48-49	22.951970481876558	26.24426020254691	27.8743850247709	22.929384290805633
50-51	22.77336512779151	26.572163415538924	27.61198109247381	23.042490364195757
52-53	22.91965186922956	26.599701869367077	27.306524495391905	23.174121766011456
54-55	22.853967141807573	26.635268700830377	27.141821327261397	23.368942830100654
56-57	22.915564393224468	26.68895794463691	27.00410930977141	23.391368352367213
58-59	22.706622529988728	26.647020825806	27.341936295633694	23.30442034857158
60-61	22.86135465042926	26.753079149447593	27.266307966855507	23.119258233267637
62-63	22.657554798754372	26.775463139633942	27.202177992212416	23.364804069399263
64-65	22.5350296791761	26.730889893952597	27.31684089108009	23.41723953579121
66-67	22.642989090056457	26.823718787292027	27.09298635681185	23.440305765839668
68-69	22.886039586328575	26.49655422785207	27.14455741269026	23.472848773129094
70-71	22.58986280637177	26.329913148178264	27.326916088164516	23.75330795728545
72-73	22.78517049475007	26.243270396483588	27.223597158317688	23.747961950448662
74-75	22.641445866634395	26.411055219718264	27.14362132221495	23.80387759143239
76-77	22.49641720415766	26.305920060941045	27.335297096190807	23.86236563871049
78-79	22.680476163629336	26.38130492595867	27.174727630498786	23.763491279913204
80-81	22.39760702025067	26.335531458910456	27.259335501893883	24.007526018944997
82-83	22.428578353163942	26.230754236929794	27.23825298129491	24.102414428611354
84-85	22.600943260022138	26.27701044323596	26.99803372910837	24.12401256763353
86-87	22.403772743038427	26.108659207026836	27.154901709373796	24.33266634056094
88-89	22.33933209673006	26.119268048197274	27.181128920703845	24.360270934368817
90-91	22.32403224048844	26.14684087008205	27.173099604573785	24.35602728485572
92-93	22.217829583413977	26.045349679450048	27.231499666369537	24.505321070766442
94-95	22.05857582372252	26.092023169075816	27.297983877898286	24.55141712930338
96-97	22.17391947038244	25.953646088113064	27.333379856148248	24.539054585356247
98-99	22.131747131747133	25.945004070004067	27.0742926992927	24.848956098956098
100-101	21.964588281276587	26.025554161050547	27.1122711742752	24.89758638339767
102-103	21.83458410905261	25.852764127257377	27.173812908885413	25.1388388548046
104-105	21.86093598531464	25.869536819959986	27.1044200452386	25.165107149486772
106-107	21.554317975801997	26.02153324604351	27.310152374476772	25.113996403677724
108-109	21.558368435127964	25.96734175318082	27.27462722890953	25.199662582781684
110-111	21.337499376983985	26.108031836548097	27.131840501025184	25.422628285442737
112-113	21.185913432519797	26.20373922237484	27.085644911366018	25.524702433739343
114-115	21.233939435603066	26.11717595297838	27.150242326332798	25.498642285085758
116-117	21.12830683511411	25.97470538597574	27.213656822997855	25.683330955912304
118-119	21.074224073543864	25.985523440800197	27.129095313008587	25.811157172647352
120-121	21.02838767375605	26.04442669396147	27.073893603192612	25.853292029089864
122-123	20.776525502084645	26.023778343765148	27.085314002687866	26.114382151462344
124-125	20.58911424121468	26.160368459478022	27.068689988723786	26.18182731058351
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	58.0
1	50.0
2	73.5
3	114.5
4	101.5
5	91.5
6	136.0
7	336.0
8	538.5
9	614.5
10	569.0
11	435.0
12	408.5
13	485.0
14	573.5
15	656.5
16	747.5
17	874.0
18	1031.5
19	1193.5
20	1375.5
21	1601.0
22	1886.0
23	2385.0
24	3046.5
25	3720.0
26	4974.0
27	6794.5
28	8674.0
29	11256.5
30	13754.5
31	16227.5
32	19467.0
33	22873.0
34	26244.5
35	29256.5
36	33029.5
37	36411.5
38	38493.5
39	40813.0
40	43605.0
41	45814.0
42	46624.5
43	48583.5
44	51607.0
45	54040.5
46	55843.5
47	57378.5
48	58898.5
49	60517.5
50	64673.5
51	63578.5
52	56199.5
53	52235.5
54	50341.5
55	47199.0
56	43209.0
57	39806.0
58	37181.5
59	33229.5
60	27399.0
61	21963.0
62	16891.0
63	12138.0
64	8931.5
65	6807.0
66	5060.0
67	3715.0
68	2731.5
69	1987.0
70	1536.0
71	1179.0
72	781.5
73	551.5
74	415.0
75	281.5
76	191.5
77	127.5
78	69.0
79	34.0
80	23.0
81	15.0
82	6.5
83	4.0
84	3.0
85	2.5
86	2.5
87	2.0
88	1.5
89	3.0
90	2.0
91	2.0
92	2.5
93	1.0
94	1.5
95	2.5
96	2.5
97	1.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0801497411630786
2	1.3747811520253622E-4
3	4.124343456076086E-4
4	0.19363792526277224
5	0.0738944869213632
6	0.01017338052498768
7	0.013335377174646012
8	0.010860771101000359
9	0.018559545552342387
10-11	0.018044002620332875
12-13	0.019762479060364577
14-15	0.026189580946083146
16-17	0.027358144925304705
18-19	0.024093039689244467
20-21	0.0272550363389028
22-23	0.02814864408771929
24-25	0.026292689532485047
26-27	0.026017733302079973
28-29	0.025467820841269834
30-31	0.026052102830880608
32-33	0.0223058241916115
34-35	0.024333626390848906
36-37	0.027117558223700267
38-39	0.03155122743898206
40-41	0.027220666810102163
42-43	0.027461253511706602
44-45	0.02845796984692499
46-47	0.028389230789323726
48-49	0.023955561574041932
50-51	0.029935859585352255
52-53	0.026189580946083146
54-55	0.030004598642953524
56-57	0.0272550363389028
58-59	0.02443673497725081
60-61	0.024574213092453345
62-63	0.028183013616519918
64-65	0.03261668283180171
66-67	0.031723075082985226
68-69	0.026533276234089485
70-71	0.03564120136625751
72-73	0.03904378471752028
74-75	0.02536471225486793
76-77	0.017631568274725267
78-79	0.021927759374804522
80-81	0.024471104506051444
82-83	0.02292447571002291
84-85	0.01697854722751322
86-87	0.01639426523790244
88-89	0.017528459688323363
90-91	0.01859391508114302
92-93	0.02223708513401023
94-95	0.017322242515519563
96-97	0.020174913405972186
98-99	0.01601620042109547
100-101	0.017769046389927802
102-103	0.014641419269070106
104-105	0.01835332837953858
106-107	0.01618804806509864
108-109	0.013610333405051082
110-111	0.010963879687402261
112-113	0.009829685236981339
114-115	0.008729860315361049
116-117	0.008214317383351537
118-119	0.005533494136902082
120-121	0.002990149005655162
122-123	0.006323993299316665
124-125	0.05715652639545442
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1454777.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.953147146964774
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.81352764631744	36.27032474657824
2	14.661124587427638	15.233831260612224
3	6.229053437970635	9.708567895475857
4	3.2283637334873245	6.708946243591661
5	1.8227204824788272	4.734803271700456
6	1.1283880908453499	3.5173987513542664
7	0.7264622717180806	2.641940090950142
8	0.49608313138462923	2.061846393756216
9	0.3697428062886831	1.7288372179482883
>10	1.4560763101180563	12.120534554534037
>50	0.045312251190443666	1.6027427737665116
>100	0.020750982251934225	1.9558591437278723
>500	0.001197134260486061	0.45042923177344263
>1k	0.001197134260486061	1.2639384242307865
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3251	0.22347067626172257	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3235	0.22237085134010232	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	2399	0.16490499918544216	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	2360	0.16222417593899272	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2051	0.14098380714020087	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT	1533	0.10537697530274398	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	3.436952880063405E-5	0.0
16-17	0.0	0.0	0.0	1.7184764400317025E-4	0.0
18-19	0.0	0.0	0.0	2.062171728038043E-4	0.0
20-21	0.0	0.0	0.0	3.0932575920570644E-4	0.0
22-23	0.0	0.0	0.0	6.530210472120469E-4	0.0
24-25	0.0	0.0	0.0	0.0018903240840348727	0.0
26-27	0.0	0.0	0.0	0.003402583351262771	0.0
28-29	0.0	0.0	0.0	0.00756129633613949	0.0
30-31	0.0	0.0	0.0	0.01900634942675063	0.0
32-33	0.0	0.0	0.0	0.04478349602722617	0.0
34-35	0.0	0.0	0.0	0.08190258713191094	0.0
36-37	0.0	0.0	0.0	0.1430116093394383	0.0
38-39	0.0	0.0	0.0	0.22835114935141262	0.0
40-41	0.0	0.0	0.0	0.32376096130197274	0.0
42-43	0.0	0.0	0.0	0.4078288287483236	0.0
44-45	0.0	0.0	0.0	0.5036510750444914	0.0
46-47	0.0	0.0	0.0	0.6202668862650427	0.0
48-49	0.0	0.0	0.0	0.7376731966480086	0.0
50-51	0.0	0.0	0.0	0.8519862494389174	0.0
52-53	0.0	0.0	0.0	0.9710079276755132	0.0
54-55	0.0	0.0	0.0	1.1086235209932518	0.0
56-57	0.0	0.0	0.0	1.2729442381890834	0.0
58-59	0.0	0.0	0.0	1.4462010328730794	0.0
60-61	0.0	0.0	0.0	1.6089751212728824	0.0
62-63	0.0	0.0	0.0	1.7763547265319701	0.0
64-65	0.0	0.0	0.0	1.9729140617427965	0.0
66-67	0.0	0.0	0.0	2.2052864459638832	0.0
68-69	0.0	0.0	0.0	2.4570432444285277	0.0
70-71	0.0	0.0	0.0	2.727222110330312	0.0
72-73	0.0	0.0	0.0	2.996026195080071	0.0
74-75	0.0	0.0	0.0	3.2891639062206783	0.0
76-77	0.0	0.0	0.0	3.629387871818155	0.0
78-79	0.0	0.0	0.0	4.003053388938649	0.0
80-81	0.0	0.0	0.0	4.386067417892914	0.0
82-83	0.0	0.0	0.0	4.784307148105861	0.0
84-85	0.0	0.0	0.0	5.216022799370625	0.0
86-87	0.0	0.0	0.0	5.687950799332132	0.0
88-89	0.0	0.0	0.0	6.209095964536145	0.0
90-91	0.0	0.0	0.0	6.736840079269881	0.0
92-93	0.0	0.0	0.0	7.273039098088573	0.0
94-95	0.0	0.0	0.0	7.843435798063895	0.0
96-97	0.0	0.0	0.0	8.444971291132592	0.0
98-99	0.0	0.0	0.0	9.10710026347681	0.0
100-101	0.0	0.0	0.0	9.805420349648092	0.0
102-103	0.0	0.0	0.0	10.493635794351988	0.0
104-105	0.0	0.0	0.0	11.200513893194628	0.0
106-107	0.0	0.0	0.0	11.940971021675487	0.0
108-109	0.0	0.0	0.0	12.72548988607876	0.0
110-111	0.0	0.0	0.0	13.537710590695344	0.0
112-113	0.0	0.0	0.0	14.362991716256168	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	8990	0.0	30.450888	1
GCGTTAG	80	0.0053008464	29.820642	1
GGTATCA	6770	0.0	29.424206	1
TATCAAC	10695	0.0	25.19388	2
TCAACGC	10805	0.0	24.885653	4
GAGTACT	5085	0.0	24.862984	12-13
ATCAACG	10875	0.0	24.669796	3
CAACGCA	11085	0.0	24.109604	5
AACGCAG	11185	0.0	24.03881	6
CGCGTGA	65	0.0020533728	22.883728	24-25
AGTACTT	5680	0.0	22.72985	12-13
GTACTTT	5675	0.0	22.331295	14-15
ACGCAGA	12220	0.0	21.711464	7
CATGGGG	4175	0.0	21.563425	4
CGCAGAG	12390	0.0	21.41504	8
GTCCTAG	415	2.910383E-11	20.11995	1
TAGGGCG	150	0.0046516173	19.873575	4
GCAGAGT	12825	0.0	19.715908	9
TACTTTT	6545	0.0	19.544699	14-15
TCTATAC	580	0.0	19.530254	3
>>END_MODULE