FastQCFastQC Report
Mon 15 Aug 2016
SRR2921126_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921126_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2516248
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA60000.031.7531031
TCAACGC66100.027.639224
CAACGCA68100.026.8269625
ATCAACG68600.026.72033
GGTATCA43950.026.551231
AACGCAG70350.025.9668926
GTACATG100550.025.697751
TATCAAC73650.025.6154082
TACATGG100600.025.0240232
CATGGGG65350.023.8585784
ACATGGG104450.023.5318643
ACGCAGA79150.022.5536087
CGCAGAG82700.021.5139438
GCAGAGT93500.018.5834169
GTATAAG11800.018.163851
ACGATAC2652.0814794E-517.9663093
GTATAAT12400.017.7650931
AGAGTAC79850.017.5889110-11
GTATATA12900.017.0765251
GTTCGCG2200.00222246916.2283467
Produced by FastQC (version 0.11.5)