FastQCFastQC Report
Mon 15 Aug 2016
SRR2921126_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921126_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2516248
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA69000.034.5802881
GGTATCA49100.030.858151
TCAACGC81150.028.6569084
ATCAACG82250.028.3455873
CAACGCA84150.027.58915
TATCAAC86000.027.3159582
AACGCAG86650.026.7765986
ACGCAGA97350.023.6497067
CGCAGAG99350.023.2334988
CATGGGG64750.023.2067344
GTACATG102250.022.9853211
TACATGG101450.022.5094762
ACATGGG105700.021.0979963
GTATAGC10500.021.0198761
GCAGAGT113000.019.9539459
AGAGTAC96900.019.33973310-11
GTATAAT10300.019.1114851
GAGTACT40000.018.74001512-13
TATACAG16300.017.5299515
TCCGGTA2752.8165146E-517.3450662
Produced by FastQC (version 0.11.5)