FastQCFastQC Report
Mon 15 Aug 2016
SRR2921125_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921125_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3875579
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA77750.040.9644361
GGTATCA52950.040.250441
TCAACGC93150.033.4768834
CAACGCA95450.032.481495
TATCAAC98000.032.0621952
ATCAACG99600.031.1886483
AACGCAG99800.031.1837626
GTACATG166150.030.348461
TACATGG171900.028.5949632
ACATGGG173300.027.8145373
CATGGGG133100.027.0951254
ACGCAGA115400.026.7616777
CGTATAC3301.8189894E-1225.24643
CGCAGAG124000.024.7139918
GCAGAGT137700.021.5202439
AGAGTAC118800.019.88693610-11
ATGGGGG67650.019.1762145
CAGAGTA130050.018.6699710-11
GTATAGA12000.018.3558311
ATGGGGA64450.017.5430415
Produced by FastQC (version 0.11.5)