##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921125_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3875579
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.39675078227021	31.0	30.0	34.0	25.0	34.0
2	29.468257001082936	31.0	30.0	34.0	23.0	34.0
3	29.532262405178685	31.0	30.0	34.0	24.0	34.0
4	32.994800260812646	35.0	35.0	37.0	26.0	37.0
5	33.042545900883454	37.0	35.0	37.0	26.0	37.0
6	32.98729815596586	37.0	35.0	37.0	26.0	37.0
7	32.958031303193664	37.0	35.0	37.0	26.0	37.0
8	32.88458240691262	37.0	33.0	37.0	26.0	37.0
9	34.23899990169211	38.0	35.0	39.0	25.0	39.0
10-11	34.18815859514153	38.0	34.5	39.0	25.0	39.0
12-13	34.16200327744577	38.0	34.5	39.0	25.0	39.0
14-15	35.15680147405072	39.0	35.0	41.0	25.0	41.0
16-17	35.040902404518135	39.0	34.0	41.0	25.0	41.0
18-19	35.085028456393225	39.0	34.0	41.0	25.0	41.0
20-21	35.10655840069316	39.0	34.0	41.0	25.0	41.0
22-23	35.05244171258023	39.0	34.0	41.0	24.0	41.0
24-25	34.94127754846437	39.0	34.0	41.0	24.0	41.0
26-27	34.870500382007435	39.0	34.0	41.0	23.5	41.0
28-29	34.719987258677996	39.0	34.0	41.0	23.0	41.0
30-31	34.61655471350217	39.0	34.0	41.0	22.0	41.0
32-33	34.52241613446662	39.0	34.0	41.0	21.5	41.0
34-35	34.422463714454025	39.0	34.0	41.0	21.0	41.0
36-37	34.27725676602128	39.0	33.5	41.0	19.5	41.0
38-39	34.114270925712006	39.0	33.0	41.0	18.0	41.0
40-41	34.00158169914741	38.0	33.0	41.0	18.0	41.0
42-43	33.9049389781501	38.0	33.0	40.5	17.5	41.0
44-45	33.724743838275515	38.0	33.0	40.0	15.5	41.0
46-47	33.47973928540742	38.0	33.0	40.0	15.0	41.0
48-49	33.33486015895947	38.0	32.5	40.0	13.5	41.0
50-51	33.14671446511605	38.0	32.0	40.0	10.5	41.0
52-53	32.917340996016335	38.0	32.0	40.0	8.5	41.0
54-55	32.6456641704375	37.0	32.0	40.0	8.0	41.0
56-57	32.353882864986105	37.0	31.0	40.0	7.0	41.0
58-59	32.1000829811494	36.0	31.0	40.0	4.0	41.0
60-61	31.7775021487112	36.0	31.0	39.5	2.0	41.0
62-63	31.482863721781957	35.5	30.5	39.0	2.0	41.0
64-65	31.13940471862398	35.0	30.0	39.0	2.0	40.0
66-67	30.84192929624193	35.0	30.0	38.0	2.0	40.0
68-69	30.470882415246855	35.0	29.5	37.5	2.0	40.0
70-71	30.156613502137358	35.0	29.0	37.0	2.0	39.5
72-73	29.80650762118383	34.0	29.0	36.5	2.0	39.0
74-75	29.748295286975186	34.0	29.0	36.0	2.0	39.0
76-77	29.51770961190573	34.0	29.0	36.0	2.0	38.5
78-79	29.287260948622126	34.0	29.0	35.0	2.0	37.0
80-81	28.970916732699813	34.0	29.0	35.0	2.0	37.0
82-83	28.754686848081278	34.0	29.0	35.0	2.0	36.0
84-85	28.606908541923673	34.0	29.0	35.0	2.0	36.0
86-87	28.427473288507343	34.0	29.0	35.0	2.0	36.0
88-89	28.297293127039858	34.0	29.0	35.0	2.0	35.0
90-91	28.16421959144685	34.0	28.0	35.0	2.0	35.0
92-93	28.385548069075615	34.0	29.0	35.0	2.0	35.0
94-95	28.3891978463089	34.0	29.0	35.0	2.0	35.0
96-97	28.3412426891569	34.0	29.0	35.0	2.0	35.0
98-99	28.240921937083463	34.0	29.0	35.0	2.0	35.0
100-101	28.145944644658258	34.0	29.0	35.0	2.0	35.0
102-103	26.800778154696367	32.0	25.5	34.0	2.0	35.0
104-105	27.847905564562097	34.0	28.0	35.0	2.0	35.0
106-107	28.02361479407335	34.0	29.0	35.0	2.0	35.0
108-109	27.962641066018783	34.0	29.0	35.0	2.0	35.0
110-111	27.93889158239324	34.0	29.0	35.0	2.0	35.0
112-113	27.851676226958602	34.0	29.0	35.0	2.0	35.0
114-115	27.719434180028326	34.0	28.5	35.0	2.0	35.0
116-117	27.54943300084968	34.0	27.0	35.0	2.0	35.0
118-119	27.425943968630236	34.0	27.0	35.0	2.0	35.0
120-121	27.23103205998381	34.0	27.0	35.0	2.0	35.0
122-123	27.06673880212479	34.0	27.0	35.0	2.0	35.0
124-125	25.970342108882313	33.0	23.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	172874.0
3	25394.0
4	16984.0
5	15347.0
6	16812.0
7	18370.0
8	20293.0
9	21747.0
10	23079.0
11	23942.0
12	24417.0
13	24054.0
14	22928.0
15	20346.0
16	17381.0
17	15461.0
18	14716.0
19	14367.0
20	14794.0
21	16069.0
22	19865.0
23	26431.0
24	35808.0
25	45468.0
26	55122.0
27	65159.0
28	76287.0
29	89638.0
30	106023.0
31	127266.0
32	157344.0
33	200279.0
34	266886.0
35	387371.0
36	645071.0
37	833654.0
38	198478.0
39	54.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.73307404187685	17.878046091535012	13.108324324659273	25.280555541928866
2	16.60047697863299	19.358690306062147	38.08226926636021	25.958563448944656
3	19.54585948927903	23.47201275099127	28.406931286898335	28.575196472831365
4	14.18796700062273	15.887227954757602	34.42391869024463	35.500886354375034
5	15.429445121833208	36.06665505428787	32.989348868115634	15.514550955763287
6	33.87629647578445	33.909378494159164	17.06957313616244	15.144751893893943
7	29.5583658325551	30.912740202429745	20.446498406155417	19.08239555885974
8	27.264645711879243	33.71024528909514	19.477202069329984	19.54790692969563
9	26.871920108150537	15.936205839024664	19.011523895070855	38.18035015775394
10-11	26.276950994391584	25.123978799834894	26.41930646191633	22.179763743857187
12-13	26.535511968066235	22.6013225913749	26.586450341150336	24.276715099408534
14-15	24.19283583907449	23.852340271581017	23.804781853173253	28.150042036171236
16-17	24.494863377148075	26.756515470512692	24.534101423575937	24.214519728763296
18-19	24.466671087796673	26.113279672512967	25.77145063038519	23.64859860930517
20-21	25.446481738993327	25.0676346722549	25.460442861782433	24.02544072696934
22-23	25.319348339471208	24.75514735025515	25.178678698825824	24.74682561144782
24-25	24.8320180336769	25.135146781661632	25.228078740450055	24.80475644421141
26-27	24.63491849691235	25.33358827239051	25.288793312545994	24.742699918151146
28-29	24.619594255376825	25.365948319964676	25.428177823871795	24.5862796007867
30-31	24.899266461144013	25.248979246375075	25.153864593996424	24.697889698484488
32-33	24.677173140062113	25.210414165883105	25.138564482284902	24.973848211769877
34-35	24.884285452184095	25.197734660484215	25.229964681161487	24.688015206170206
36-37	24.987742954004855	25.104030065114593	25.149858514982753	24.7583684658978
38-39	24.811008622741667	25.308130612571382	25.167697391889966	24.71316337279698
40-41	24.853465279089427	25.21452538645017	25.315943720758856	24.61606561370155
42-43	24.81320896122211	25.06090472234633	25.2141357528621	24.911750563569463
44-45	24.80613694224497	25.134057570409656	25.185380238656858	24.874425248688517
46-47	24.863978960685852	25.147806884507617	25.18527387349705	24.802940281309482
48-49	24.787493105661582	25.340949628592718	25.085547853965817	24.786009411779887
50-51	24.687583015849114	25.471568728769068	25.095387186242828	24.745461069138987
52-53	24.855585073819242	25.247908043046635	25.07882778417644	24.81767909895769
54-55	24.75273405305593	25.165157912174852	25.11575633060173	24.966351704167487
56-57	24.75826320037487	25.257167082613424	25.06290807679843	24.921661640213276
58-59	24.747823874599856	25.141634580202744	25.194312116271956	24.91622942892545
60-61	24.74315149130248	25.249165406040042	25.230522109079416	24.77716099357806
62-63	24.67637690762679	25.275203483631515	25.165772329528917	24.882647279212776
64-65	24.712888736362093	25.132774646606237	25.15759752235704	24.996739094674627
66-67	24.71646271489055	25.21107657406429	25.06516174420214	25.007298966843017
68-69	24.706990970632205	25.198782976376982	25.118471183419107	24.975754869571713
70-71	24.56372259433481	25.124392198309163	25.28556955029158	25.026315657064448
72-73	24.709854053915276	25.177573563610327	25.20074472846324	24.91182765401116
74-75	24.620087534556852	25.350303367464	25.176093498177526	24.853515599801625
76-77	24.73483489914876	25.063217486214718	25.209688531315877	24.992259083320647
78-79	24.636641014559128	25.143107796907554	25.261683750153203	24.95856743838011
80-81	24.674035549812466	25.251214271117377	25.21296189263222	24.86178828643794
82-83	24.638111950814036	25.215716266248872	25.268482602719544	24.87768918021755
84-85	24.59900409475304	25.224847599817267	25.16174719612176	25.014401109307936
86-87	24.62234597172907	25.152164674573115	25.258574800977712	24.9669145527201
88-89	24.494462503399493	25.0622616807093	25.291750030576083	25.151525785315123
90-91	24.508097148232952	25.146069137843753	25.298885684192385	25.046948029730913
92-93	24.516248392784295	25.17575192407878	25.369082265963204	24.93891741717372
94-95	24.459120535460865	25.060159358437794	25.460348751399803	25.02037135470154
96-97	24.56629404017802	24.954806437895815	25.407645156096475	25.07125436582969
98-99	24.451419515382085	25.004128448415553	25.32670218508454	25.217749851117826
100-101	24.35356252610127	25.10435025717131	25.505276117753716	25.0368110989737
102-103	24.215825067820447	25.08980476148761	25.54929498601532	25.145075184676625
104-105	24.180558727258887	25.17972913868579	25.421135542903144	25.218576591152175
106-107	24.248311115927894	25.160295612772043	25.43716164259136	25.154231628708708
108-109	24.200680697812025	25.16579396723887	25.445977320567177	25.187548014381928
110-111	24.164448399499435	25.271111148958525	25.47227446670948	25.092165984832558
112-113	24.191180938724887	25.2728919678476	25.49695450667343	25.03897258675408
114-115	24.097183453127418	25.436204731441613	25.434282314759994	25.032329500670976
116-117	24.139550407645384	25.413609148885357	25.38734149256281	25.059498950906445
118-119	24.19717618633176	25.402324683095696	25.331792558402842	25.068706572169702
120-121	24.14832548663567	25.46401870563614	25.377176270017397	25.01047953771079
122-123	24.13491122306064	25.535781188557205	25.313090560425394	25.01621702795676
124-125	24.20298616462383	25.613664628994172	25.184562926480584	24.998786279901413
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	487.0
1	389.0
2	249.0
3	173.0
4	137.0
5	131.0
6	138.0
7	170.5
8	215.5
9	258.5
10	264.0
11	266.5
12	293.0
13	314.5
14	367.0
15	429.0
16	484.5
17	532.5
18	606.5
19	693.5
20	780.0
21	897.0
22	1029.5
23	1217.5
24	1541.5
25	1957.0
26	2537.5
27	3497.0
28	4957.5
29	7277.5
30	10799.5
31	15340.0
32	21072.5
33	27226.5
34	33494.0
35	40823.5
36	49824.5
37	58753.0
38	67465.5
39	76859.5
40	86018.5
41	95311.5
42	104975.5
43	116442.5
44	127985.5
45	138889.5
46	150727.0
47	160758.0
48	169730.0
49	176588.5
50	179056.0
51	180483.0
52	179981.0
53	177942.5
54	174983.0
55	168159.0
56	156916.5
57	142511.5
58	130112.5
59	118421.5
60	103342.0
61	88269.5
62	73686.5
63	57447.5
64	43288.0
65	33060.0
66	24989.0
67	19092.5
68	14926.5
69	11627.5
70	8848.0
71	6857.5
72	5247.5
73	4029.5
74	3107.5
75	2377.0
76	1776.0
77	1175.5
78	752.0
79	510.5
80	361.0
81	260.0
82	197.5
83	145.5
84	105.0
85	73.5
86	48.5
87	36.5
88	28.5
89	21.5
90	21.0
91	20.0
92	18.0
93	15.5
94	14.0
95	12.5
96	8.5
97	11.0
98	12.0
99	13.5
100	27.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06347438666583755
2	0.00910831646058563
3	0.019171328980779386
4	0.018526264075638763
5	0.010424248867072506
6	0.009340539826436256
7	0.009237329441613757
8	0.008127817804771882
9	0.006192623089350004
10-11	0.01084999170446532
12-13	0.007947199631332506
14-15	0.008901895690940631
16-17	0.013249633151588447
18-19	0.006450649051406255
20-21	0.013739882479495324
22-23	0.004825085490451878
24-25	0.004205823181516878
26-27	0.0036897712574043774
28-29	0.010514557953792195
30-31	0.006605464628640005
32-33	0.0032769297181143775
34-35	0.007998804823743755
36-37	0.00663126722484563
38-39	0.0018448856287021887
40-41	0.0012127220216643759
42-43	0.0015094518780290635
44-45	0.0025415557262540643
46-47	0.004321934864442191
48-49	0.0030060024579553145
50-51	0.004889591980965941
52-53	0.005173420539227816
54-55	0.005083111452508129
56-57	0.0021416154850668768
58-59	0.002915693371235627
60-61	0.004941197173377191
62-63	0.0021416154850668768
64-65	0.0031350154389834396
66-67	7.86979184271563E-4
68-69	0.0015094518780290635
70-71	8.901895690940631E-4
72-73	0.0018706882249078139
74-75	0.0020900102926556265
76-77	0.0017803791381881262
78-79	1.0321038482250007E-4
80-81	7.740778861687505E-5
82-83	5.160519241125004E-5
84-85	2.451246639534377E-4
86-87	2.0642076964500015E-4
88-89	2.580259620562502E-5
90-91	4.3864413549562527E-4
92-93	0.002270628466095002
94-95	0.0010063012520193757
96-97	6.966700975518755E-4
98-99	7.998804823743756E-4
100-101	0.0012772285121784384
102-103	0.002283529764197814
104-105	0.004231625777722503
106-107	0.0061668204931443794
108-109	0.011301537138063757
110-111	0.009534059297978443
112-113	0.0025415557262540643
114-115	0.006411945157097818
116-117	0.002335134956609064
118-119	0.0010321038482250006
120-121	0.004373540056853441
122-123	5.934597127293754E-4
124-125	0.08223287410732692
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	3875579.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.84346446887027
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.12953068936271	40.45234216235599
2	12.799896392909188	13.783815332736532
3	4.654646747334852	7.518669202658
4	2.2627272641193383	4.873323001934145
5	1.2644703814018063	3.4041733026473495
6	0.8072146211838885	2.6077939064680367
7	0.5484900128171364	2.067282176165478
8	0.4074061022475501	1.7548924792613514
9	0.3035366901978225	1.4709120294298443
>10	1.7067065026589636	16.62285925912091
>50	0.0903852907527517	3.3063977520205414
>100	0.024701047982079442	2.0068287536316842
>500	1.9217135919986924E-4	0.0728699556994934
>1k	9.608567259364855E-5	0.057840685870587036
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.580259620562502E-5
2	0.0	0.0	0.0	0.0	2.580259620562502E-5
3	0.0	0.0	0.0	0.0	2.580259620562502E-5
4	0.0	0.0	0.0	0.0	5.160519241125004E-5
5	0.0	0.0	0.0	0.0	7.740778861687505E-5
6	0.0	0.0	0.0	0.0	7.740778861687505E-5
7	0.0	0.0	0.0	0.0	7.740778861687505E-5
8	0.0	0.0	0.0	0.0	7.740778861687505E-5
9	0.0	0.0	0.0	0.0	7.740778861687505E-5
10-11	0.0	0.0	0.0	0.0	1.0321038482250007E-4
12-13	0.0	0.0	0.0	2.580259620562502E-5	2.3222336585062515E-4
14-15	0.0	0.0	0.0	2.580259620562502E-5	3.4833504877593773E-4
16-17	0.0	0.0	0.0	2.580259620562502E-5	4.128415392900003E-4
18-19	0.0	0.0	0.0	5.160519241125004E-5	5.676571165237503E-4
20-21	0.0	0.0	0.0	7.740778861687505E-5	5.934597127293754E-4
22-23	0.0	0.0	0.0	1.290129810281251E-4	7.224726937575005E-4
24-25	0.0	0.0	0.0	6.063610108321879E-4	8.256830785800006E-4
26-27	0.0	0.0	0.0	0.0015223531761318762	8.514856747856256E-4
28-29	0.0	0.0	0.0	0.003638166064993127	8.901895690940631E-4
30-31	0.0	0.0	0.0	0.012049812428026883	9.030908671968756E-4
32-33	0.0	0.0	0.0	0.030111629771964396	9.288934634025006E-4
34-35	0.0	0.0	0.0	0.05379841308872816	0.0010063012520193757
36-37	0.0	0.0	0.0	0.09273453076301631	0.0010063012520193757
38-39	0.0	0.0	0.0	0.15471236684892758	0.0010321038482250008
40-41	0.0	0.0	0.0	0.22738537906207046	0.0010321038482250008
42-43	0.0	0.0	0.0	0.2972201056925946	0.0010321038482250008
44-45	0.0	0.0	0.0	0.38106564206277305	0.0010966103387390633
46-47	0.0	0.0	0.0	0.4767287674951278	0.0011611168292531258
48-49	0.0	0.0	0.0	0.5734239967757075	0.0011869194254587507
50-51	0.0	0.0	0.0	0.6719770129830923	0.0012127220216643759
52-53	0.0	0.0	0.0	0.7740907874668533	0.0012643272140756257
54-55	0.0	0.0	0.0	0.8945889117471222	0.0012901298102812508
56-57	0.0	0.0	0.0	1.0342454637100675	0.0012901298102812508
58-59	0.0	0.0	0.0	1.1811525452067935	0.0012901298102812508
60-61	0.0	0.0	0.0	1.3276596864623325	0.0012901298102812508
62-63	0.0	0.0	0.0	1.484358853219093	0.001444945387515001
64-65	0.0	0.0	0.0	1.6631321410297661	0.001444945387515001
66-67	0.0	0.0	0.0	1.8721976767858428	0.001444945387515001
68-69	0.0	0.0	0.0	2.1003313311378764	0.0014578466856178134
70-71	0.0	0.0	0.0	2.3350704501185504	0.001470747983720626
72-73	0.0	0.0	0.0	2.584233220378168	0.001625563560954376
74-75	0.0	0.0	0.0	2.8539606598136693	0.001677168753365626
76-77	0.0	0.0	0.0	3.1578765392216237	0.0017287739457768762
78-79	0.0	1.290129810281251E-5	0.0	3.490394596523513	0.0017287739457768762
80-81	0.0	2.580259620562502E-5	0.0	3.834949564955327	0.0017674778400853138
82-83	0.0	2.580259620562502E-5	0.0	4.19198008865256	0.0019351947154218764
84-85	0.0	2.580259620562502E-5	0.0	4.576090437067597	0.0019609973116275015
86-87	0.0	2.580259620562502E-5	0.0	4.997885477240949	0.002025503802141564
88-89	0.0	2.580259620562502E-5	0.0	5.457352307874514	0.002102911590758439
90-91	0.0	2.580259620562502E-5	0.0	5.934958363640632	0.0021158128888612514
92-93	0.0	2.580259620562502E-5	0.0	6.420460013845673	0.0021416154850668763
94-95	0.0	2.580259620562502E-5	0.0	6.936821569112641	0.002231924571786564
96-97	0.0	2.580259620562502E-5	0.0	7.491783808303224	0.0024125427452259393
98-99	0.0	2.580259620562502E-5	0.0	8.086740071612525	0.002528654428151252
100-101	0.0	2.580259620562502E-5	0.0	8.710853268634184	0.002528654428151252
102-103	0.0	2.580259620562502E-5	0.0	9.332076574880812	0.002606062216768127
104-105	0.0	2.580259620562502E-5	0.0	9.973787142514706	0.0026705687072821893
106-107	0.0	2.580259620562502E-5	0.0	10.660381326248284	0.0026963713034878142
108-109	0.0	2.580259620562502E-5	0.0	11.383305565439382	0.0027479764958990645
110-111	0.0	2.580259620562502E-5	0.0	12.127297624432376	0.0027995816883103148
112-113	0.0	2.580259620562502E-5	0.0	12.869612514672001	0.002864088178824377
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7775	0.0	40.964436	1
GGTATCA	5295	0.0	40.25044	1
TCAACGC	9315	0.0	33.476883	4
CAACGCA	9545	0.0	32.48149	5
TATCAAC	9800	0.0	32.062195	2
ATCAACG	9960	0.0	31.188648	3
AACGCAG	9980	0.0	31.183762	6
GTACATG	16615	0.0	30.34846	1
TACATGG	17190	0.0	28.594963	2
ACATGGG	17330	0.0	27.814537	3
CATGGGG	13310	0.0	27.095125	4
ACGCAGA	11540	0.0	26.761677	7
CGTATAC	330	1.8189894E-12	25.2464	3
CGCAGAG	12400	0.0	24.713991	8
GCAGAGT	13770	0.0	21.520243	9
AGAGTAC	11880	0.0	19.886936	10-11
ATGGGGG	6765	0.0	19.176214	5
CAGAGTA	13005	0.0	18.66997	10-11
GTATAGA	1200	0.0	18.355831	1
ATGGGGA	6445	0.0	17.543041	5
>>END_MODULE