FastQCFastQC Report
Mon 15 Aug 2016
SRR2921125_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921125_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3875579
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63750.042.0960241
GTATCAA89350.036.7093621
TCAACGC109600.029.3766374
CAACGCA110450.029.0469915
GTACATG164100.029.0003361
TATCAAC113300.028.5740572
TACATGG169600.027.7365952
ATCAACG116550.027.6248783
AACGCAG116950.027.5194476
ACATGGG170650.026.833283
CATGGGG131200.025.585474
ACGCAGA136650.023.3779987
CGCAGAG145750.021.7553928
GTATACG2755.3918484E-821.685831
AGAGTAC136200.019.63440110-11
GCAGAGT159600.019.4206739
ATGGGGG65150.018.911855
CAGAGTA147400.018.66720610-11
ATGGGGA60600.016.3047285
GTATAAT12900.016.1803191
Produced by FastQC (version 0.11.5)