##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921125_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3875579
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.58815237671584	31.0	30.0	34.0	27.0	34.0
2	30.71533414749125	31.0	31.0	34.0	27.0	34.0
3	30.79026772515797	31.0	31.0	34.0	27.0	34.0
4	34.310354917291065	37.0	35.0	37.0	30.0	37.0
5	33.922208010725626	37.0	35.0	37.0	29.0	37.0
6	33.79024605097716	37.0	35.0	37.0	29.0	37.0
7	33.61315612454294	37.0	35.0	37.0	28.0	37.0
8	33.60867343950414	37.0	35.0	37.0	28.0	37.0
9	35.03221892780408	39.0	35.0	39.0	28.0	39.0
10-11	34.97841058071581	39.0	35.0	39.0	27.5	39.0
12-13	34.84968594370028	39.0	35.0	39.0	27.0	39.0
14-15	35.94887705811183	40.0	36.0	41.0	27.0	41.0
16-17	35.88055604078771	40.0	36.0	41.0	27.0	41.0
18-19	35.90595998688196	40.0	36.0	41.0	27.0	41.0
20-21	35.894101113665855	40.0	36.0	41.0	27.0	41.0
22-23	35.84646500561594	40.0	36.0	41.0	27.0	41.0
24-25	35.74103998396111	39.0	35.0	41.0	27.0	41.0
26-27	35.63400230004342	39.0	35.0	41.0	26.5	41.0
28-29	35.54242191940868	39.0	35.0	41.0	26.0	41.0
30-31	35.4717409966356	39.0	35.0	41.0	25.5	41.0
32-33	35.35209139073156	39.0	35.0	41.0	25.0	41.0
34-35	35.24207789339348	39.0	35.0	41.0	25.0	41.0
36-37	35.11427724734807	39.0	35.0	41.0	25.0	41.0
38-39	34.94294904064657	39.0	34.0	41.0	24.0	41.0
40-41	34.80149727305262	39.0	34.0	41.0	24.0	41.0
42-43	34.6777598908447	39.0	34.0	41.0	23.5	41.0
44-45	34.51809806999161	38.5	33.5	41.0	23.0	41.0
46-47	34.31850028086126	38.0	33.0	41.0	22.0	41.0
48-49	34.13445049113952	38.0	33.0	40.5	21.5	41.0
50-51	33.913882545033914	38.0	33.0	40.0	20.0	41.0
52-53	33.71668594550646	38.0	33.0	40.0	19.5	41.0
54-55	33.48706902891155	38.0	33.0	40.0	18.5	41.0
56-57	33.19449377757491	37.0	32.0	40.0	17.5	41.0
58-59	32.94205304549333	37.0	32.0	40.0	15.5	41.0
60-61	32.64601224746032	36.0	32.0	40.0	13.5	41.0
62-63	32.324246777062214	36.0	31.5	39.0	12.5	41.0
64-65	32.00973299731472	35.0	31.0	39.0	9.5	41.0
66-67	31.660630321301667	35.0	31.0	38.5	8.0	40.0
68-69	31.287357192305976	35.0	31.0	38.0	7.0	40.0
70-71	30.92607349766319	35.0	30.0	37.0	7.0	39.5
72-73	30.734414393307425	35.0	30.0	37.0	4.0	39.0
74-75	30.535185065250896	35.0	30.0	36.0	2.0	39.0
76-77	30.32036387337221	35.0	30.0	36.0	2.0	38.5
78-79	30.018873438007585	35.0	30.0	35.0	2.0	37.0
80-81	29.75022841748291	34.0	30.0	35.0	2.0	37.0
82-83	29.564205632242306	34.0	30.0	35.0	2.0	36.0
84-85	29.437422124539324	34.0	30.0	35.0	2.0	36.0
86-87	29.258139493479554	34.0	30.0	35.0	2.0	36.0
88-89	29.13587634260584	34.0	29.0	35.0	2.0	35.0
90-91	29.000326402842	34.0	29.0	35.0	2.0	35.0
92-93	28.897437776394185	34.0	29.0	35.0	2.0	35.0
94-95	28.807460253035742	34.0	29.0	35.0	2.0	35.0
96-97	28.75178689429373	34.0	29.0	35.0	2.0	35.0
98-99	28.712748598338468	34.0	29.0	35.0	2.0	35.0
100-101	28.572686171537207	34.0	29.0	35.0	2.0	35.0
102-103	27.18880946563081	32.0	26.0	34.0	2.0	35.0
104-105	28.30068552337599	34.0	29.0	35.0	2.0	35.0
106-107	28.59889515863307	34.0	29.0	35.0	2.0	35.0
108-109	28.821255095045153	34.0	30.0	35.0	2.0	35.0
110-111	28.801490177338664	34.0	30.0	35.0	2.0	35.0
112-113	28.73217511499572	34.0	29.5	35.0	2.0	35.0
114-115	28.62183057034833	34.0	29.0	35.0	2.0	35.0
116-117	28.526445080851147	34.0	29.0	35.0	2.0	35.0
118-119	28.389634426236697	34.0	29.0	35.0	2.0	35.0
120-121	28.21882743197855	34.0	29.0	35.0	2.0	35.0
122-123	28.039902940954114	34.0	29.0	35.0	2.0	35.0
124-125	27.23042956936241	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	96369.0
3	33140.0
4	16830.0
5	13658.0
6	14601.0
7	16864.0
8	19484.0
9	21117.0
10	22580.0
11	23029.0
12	23604.0
13	24121.0
14	24701.0
15	21416.0
16	17788.0
17	16702.0
18	16321.0
19	16300.0
20	15491.0
21	16269.0
22	17815.0
23	22439.0
24	30442.0
25	40894.0
26	51244.0
27	60947.0
28	72549.0
29	86462.0
30	102009.0
31	124312.0
32	152238.0
33	192639.0
34	256236.0
35	373102.0
36	653347.0
37	924562.0
38	243821.0
39	136.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.05523160261298	18.53972519758863	13.667744348496536	25.737298851301855
2	16.76591223179443	19.73169379900567	37.031462710536665	26.47093125866324
3	19.215272722956637	23.480124049073645	28.870566676377084	28.434036551592634
4	14.415191746322403	16.289913708403525	34.088993330191826	35.20590121508225
5	15.346920143923526	36.102926596522536	33.04233123892379	15.50782202063014
6	34.00938939998519	34.06788928941084	16.761444056997007	15.161277253606956
7	29.43602058885615	30.82209880194245	20.474665725273063	19.267214883928336
8	27.26343968926218	33.72370820951062	19.4274616506553	19.5853904505719
9	26.771893531285983	15.403371368196991	18.909376479083406	38.91535862143362
10-11	26.27984956472302	24.88088045876927	26.58954875868702	22.249721217820692
12-13	26.63499901221384	22.248338086879503	26.757068993927856	24.3595939069788
14-15	24.20860515828413	23.610411752697	23.886557598585448	28.294425490433422
16-17	24.505394047152325	26.58957210245631	24.625859680700902	24.27917416969046
18-19	24.521574784128276	25.795990284514247	25.975387642564897	23.70704728879258
20-21	25.396967143408354	24.781581932423382	25.69168409873108	24.12976682543718
22-23	25.252771622059	24.564440954913298	25.434074961942947	24.74871246108476
24-25	24.755681746326097	24.93144377537871	25.534273982806294	24.778600495488902
26-27	24.591664880463316	25.18511884629287	25.594115323482608	24.629100949761206
28-29	24.49551348882718	25.15756311331988	25.730957475542056	24.615965922310888
30-31	24.859113725646065	24.96636408755635	25.593438667788167	24.581083519009418
32-33	24.63803911199686	25.042896941396787	25.393479510056732	24.925584436549627
34-35	24.749238242768147	25.104423844149863	25.559903824762024	24.586434088319965
36-37	24.93039144937546	24.91075032700791	25.442505969765577	24.716352253851053
38-39	24.766886560493344	25.089448555014155	25.486849991250022	24.656814893242483
40-41	24.7663583362832	24.977509824248198	25.648040331715087	24.608091507753514
42-43	24.86471537803491	24.81414132576284	25.495748953450065	24.825394342752187
44-45	24.740348146800308	24.906743166564073	25.43531254879643	24.917596137839187
46-47	24.8771978592428	24.90100785316835	25.41934303258278	24.802451255006066
48-49	24.744896181514527	25.115992648529563	25.33464753384802	24.80446363610789
50-51	24.656150548347217	25.264301034748875	25.32295573215774	24.756592684746163
52-53	24.872255209803946	25.097599136099884	25.277079340367663	24.753066313728507
54-55	24.6653628029757	25.120996700764575	25.276802174281965	24.93683832197776
56-57	24.71687032061253	25.17867118784578	25.159159020422088	24.945299471119604
58-59	24.57617210637308	25.20620694585569	25.339793998235184	24.877826949536043
60-61	24.630419437386358	25.31845263827574	25.329344099189722	24.721783825148176
62-63	24.54162393437042	25.37018847894706	25.25004119980544	24.838146386877078
64-65	24.5712696458393	25.160819053076356	25.24360969792707	25.024301603157273
66-67	24.555162852068367	25.219647093410575	25.17681336133679	25.04837669318426
68-69	24.560684904264935	25.182395985410643	25.244378680585477	25.012540429738944
70-71	24.42807676984032	25.086784683769736	25.35261420105498	25.13252434533496
72-73	24.5744230357959	25.040968578903673	25.284360076411872	25.100248308888567
74-75	24.456536046394586	25.18083203642192	25.268505829003846	25.094126088179653
76-77	24.48124786201484	24.955756368986012	25.32450598429385	25.238489784705298
78-79	24.401618807526976	24.999883862096304	25.410095842159812	25.188401488216904
80-81	24.334673477723083	25.155278020277024	25.335929742107698	25.174118759892195
82-83	24.32085964503639	25.081656342004933	25.325770446024098	25.271713566934583
84-85	24.23611599472763	25.084598575324506	25.267905703798853	25.411379726149008
86-87	24.265610925179846	25.034332802959327	25.349150702649865	25.35090556921097
88-89	24.08202771410923	24.966879666508856	25.363639777226965	25.58745284215495
90-91	24.065179215356896	24.97491801821181	25.433510198672522	25.52639256775877
92-93	24.047737121931537	25.01376565823461	25.451647149563506	25.486850070270346
94-95	24.02872861306117	24.886741727007205	25.52055449423305	25.563975165698572
96-97	24.07286587592583	24.778173529581036	25.486216834423164	25.662743760069972
98-99	23.929893408064423	24.81443898390424	25.40614900470235	25.849518603328985
100-101	23.839092862119063	24.843604370057978	25.524195208172873	25.793107559650092
102-103	23.674246749803064	24.785975071984662	25.62044874040554	25.919329437806738
104-105	23.61898715638474	24.919078191403223	25.464681832287816	25.997252819924217
106-107	23.60749585061357	24.864292515259148	25.506000626327346	26.02221100779993
108-109	23.5108372947092	24.856881439238464	25.553406606905277	26.07887465914705
110-111	23.430102067477694	24.98870683776619	25.503515041098595	26.077676053657523
112-113	23.341364769563498	24.949028292555536	25.56741103466179	26.14219590321917
114-115	23.250877720624278	25.078250062931644	25.52098433218205	26.14988788426203
116-117	23.15450100825506	25.048167742057014	25.540496062263703	26.256835187424223
118-119	23.17314893839374	24.973386463641283	25.483388392485633	26.370076205479336
120-121	23.067304339097785	24.996719734502985	25.567492381849483	26.368483544549747
122-123	22.93353625872058	25.072755357240545	25.492501772758658	26.501206611280214
124-125	22.87686164115634	25.134117678646295	25.4655780975375	26.52344258265986
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	38.0
1	51.0
2	100.0
3	168.0
4	160.5
5	125.0
6	153.0
7	326.5
8	519.5
9	576.5
10	495.0
11	386.5
12	389.5
13	406.5
14	418.5
15	470.5
16	550.5
17	637.0
18	721.0
19	809.5
20	896.0
21	1007.5
22	1183.0
23	1372.5
24	1623.5
25	2029.0
26	2700.5
27	3763.0
28	5279.0
29	7677.5
30	11080.0
31	15457.0
32	21321.0
33	27065.5
34	32795.5
35	39925.5
36	48477.0
37	57397.5
38	66248.5
39	75388.0
40	84684.0
41	94211.5
42	104321.0
43	116054.0
44	127798.0
45	138654.5
46	150324.0
47	161000.5
48	170342.0
49	177678.0
50	180863.5
51	182316.5
52	181426.0
53	179692.0
54	177507.5
55	170690.0
56	158813.0
57	144009.0
58	131067.0
59	119419.0
60	104101.0
61	88496.5
62	73594.0
63	56679.5
64	42143.0
65	31722.5
66	23786.0
67	18210.5
68	13940.0
69	10673.5
70	8209.0
71	6055.0
72	4468.0
73	3568.0
74	2811.0
75	2076.0
76	1498.0
77	940.0
78	570.5
79	351.0
80	225.5
81	134.0
82	89.5
83	66.0
84	38.5
85	19.5
86	14.0
87	15.0
88	12.0
89	6.5
90	3.0
91	2.0
92	3.0
93	3.0
94	2.5
95	5.0
96	4.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07121516552752505
2	1.8061817343937512E-4
3	4.128415392900003E-4
4	0.17937964882150512
5	0.06221005945176192
6	0.009237329441613757
7	0.012204628005260634
8	0.010708077425334382
9	0.01839725109461064
10-11	0.017803791381881262
12-13	0.019416453644732826
14-15	0.025699385820802518
16-17	0.02657667409179377
18-19	0.024254440433287516
20-21	0.026163832552503766
22-23	0.02923434150097315
24-25	0.02637025332214877
26-27	0.025118827406175957
28-29	0.026331549427840332
30-31	0.02580259620562502
32-33	0.022848198940080954
34-35	0.02541555726254064
36-37	0.02722173899693439
38-39	0.03315633612422815
40-41	0.02862798049014096
42-43	0.027415258468476582
44-45	0.027453962362785016
46-47	0.029814899915599706
48-49	0.02528654428151252
50-51	0.031195338812600646
52-53	0.027273344189345645
54-55	0.030872806360030335
56-57	0.027699087026738452
58-59	0.024564071587755015
60-61	0.025389754666335015
62-63	0.030124531070067205
64-65	0.03480770228138815
66-67	0.033440164682490024
68-69	0.02865378308634658
70-71	0.038174941086222214
72-73	0.04060038512955097
74-75	0.025518767647363143
76-77	0.0187713887395922
78-79	0.02257727167992189
80-81	0.025712287118905327
82-83	0.02468018327068033
84-85	0.01706841739002095
86-87	0.016526562869702825
88-89	0.01789410046860095
90-91	0.020267939319518452
92-93	0.023235237883165328
94-95	0.017971508257217828
96-97	0.021054918503790016
98-99	0.01826823811358251
100-101	0.018332744604096576
102-103	0.01603631354179595
104-105	0.017971508257217828
106-107	0.015700879791122824
108-109	0.014384947384635947
110-111	0.01152085920581157
112-113	0.010063012520193756
114-115	0.008837389200426569
116-117	0.008243929487697194
118-119	0.005199223135433442
120-121	0.0033672388048340647
122-123	0.006786082802079379
124-125	0.054572490974896914
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	3875579.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.19479385550666
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.80131309437077	38.520443232263865
2	13.381058020360697	13.968431298826012
3	5.002877296555535	7.833724475343316
4	2.3583837428806422	4.923814131673334
5	1.335662195592438	3.485730647977036
6	0.8663522813895923	2.71314472400266
7	0.5878549407709142	2.1478077215335256
8	0.4182141232360841	1.7462879959815092
9	0.3085053044014307	1.4492133689906705
>10	1.814514107545463	17.25874576352579
>50	0.09811525323249698	3.4700985800676465
>100	0.02660417732031738	2.075432848677111
>500	9.91749741173954E-5	0.03142085315312808
>1k	4.462873695068279E-4	0.37570435798449203
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.160519241125004E-5
2	0.0	0.0	0.0	0.0	5.160519241125004E-5
3	0.0	0.0	0.0	0.0	5.160519241125004E-5
4	0.0	0.0	0.0	0.0	7.740778861687505E-5
5	0.0	0.0	0.0	2.580259620562502E-5	7.740778861687505E-5
6	0.0	0.0	0.0	2.580259620562502E-5	1.0321038482250007E-4
7	0.0	0.0	0.0	2.580259620562502E-5	1.0321038482250007E-4
8	0.0	0.0	0.0	2.580259620562502E-5	1.0321038482250007E-4
9	0.0	0.0	0.0	2.580259620562502E-5	1.548155772337501E-4
10-11	0.0	0.0	0.0	3.8703894308437525E-5	1.548155772337501E-4
12-13	0.0	0.0	0.0	7.740778861687505E-5	3.6123634687875025E-4
14-15	0.0	0.0	0.0	7.740778861687505E-5	4.386441354956253E-4
16-17	0.0	0.0	0.0	7.740778861687505E-5	5.289532222153129E-4
18-19	0.0	0.0	0.0	1.0321038482250007E-4	5.934597127293754E-4
20-21	0.0	0.0	0.0	1.290129810281251E-4	6.450649051406254E-4
22-23	0.0	0.0	0.0	1.8061817343937512E-4	6.708675013462504E-4
24-25	0.0	0.0	0.0	6.321636070378129E-4	6.708675013462504E-4
26-27	0.0	0.0	0.0	0.0015223531761318762	6.837687994490629E-4
28-29	0.0	0.0	0.0	0.0036510673630959396	7.09571395654688E-4
30-31	0.0	0.0	0.0	0.012101417620438133	7.224726937575005E-4
32-33	0.0	0.0	0.0	0.029840702511805334	7.998804823743756E-4
34-35	0.0	0.0	0.0	0.0533210650589241	8.772882709912506E-4
36-37	0.0	0.0	0.0	0.09171532821289413	8.772882709912506E-4
38-39	0.0	0.0	0.0	0.15284167862401982	9.15992165299688E-4
40-41	0.0	0.0	0.0	0.22383752208379704	9.288934634025006E-4
42-43	0.0	0.0	0.0	0.29187896827803017	9.288934634025006E-4
44-45	0.0	0.0	0.0	0.3736731982498615	9.675973577109381E-4
46-47	0.0	0.0	0.0	0.46716890560094376	0.0010321038482250006
48-49	0.0	0.0	0.0	0.5618257297812791	0.0011095116368418758
50-51	0.0	0.0	0.0	0.6584435512732421	0.0011095116368418758
52-53	0.0	0.0	0.0	0.758699538830198	0.0011869194254587507
54-55	0.0	0.0	0.0	0.8771076528178112	0.0012772285121784384
56-57	0.0	1.290129810281251E-5	0.0	1.0136033867455676	0.0013030311083840635
58-59	0.0	2.580259620562502E-5	0.0	1.1569239073697117	0.0013417350026925009
60-61	0.0	2.580259620562502E-5	0.0	1.300450848763501	0.0013417350026925009
62-63	0.0	2.580259620562502E-5	0.0	1.4542085195528203	0.001444945387515001
64-65	0.0	2.580259620562502E-5	0.0	1.6297306802415847	0.001470747983720626
66-67	0.0	2.580259620562502E-5	0.0	1.8353386681061075	0.0015094518780290635
68-69	0.0	3.8703894308437525E-5	0.0	2.0604534187020827	0.001548155772337501
70-71	0.0	5.160519241125004E-5	0.0	2.2918252988779226	0.0016126622628515636
72-73	0.0	5.160519241125004E-5	0.0	2.536653232974995	0.0017029713495712512
74-75	0.0	5.160519241125004E-5	0.0	2.8019426258631293	0.0017545765419825013
76-77	0.0	6.450649051406254E-5	0.0	3.1013688535313046	0.0017803791381881262
78-79	0.0	7.740778861687505E-5	0.0	3.428945713659817	0.0018061817343937511
80-81	0.0	7.740778861687505E-5	0.0	3.7694496744873476	0.001831984330599376
82-83	0.0	7.740778861687505E-5	0.0	4.121913138656185	0.0019609973116275015
84-85	0.0	7.740778861687505E-5	0.0	4.501146796388358	0.0019609973116275015
86-87	0.0	7.740778861687505E-5	0.0	4.917807119916792	0.0020513063983471887
88-89	0.0	7.740778861687505E-5	0.0	5.370565275536894	0.002154516783169689
90-91	0.0	7.740778861687505E-5	0.0	5.839411349891203	0.0021674180812725013
92-93	0.0	7.740778861687505E-5	0.0	6.316604564118032	0.0022190232736837515
94-95	0.0	7.740778861687505E-5	0.0	6.824322249656117	0.0022964310623006267
96-97	0.0	7.740778861687505E-5	0.0	7.370963151570384	0.0024770492357400016
98-99	0.0	7.740778861687505E-5	0.0	7.958023820440765	0.002528654428151252
100-101	0.0	7.740778861687505E-5	0.0	8.575789578795838	0.0025802596205625017
102-103	0.0	7.740778861687505E-5	0.0	9.19014939445177	0.002657667409179377
104-105	0.0	7.740778861687505E-5	0.0	9.826583331161615	0.0027092726015906267
106-107	0.0	7.740778861687505E-5	0.0	10.508107304740788	0.002747976495899064
108-109	0.0	7.740778861687505E-5	0.0	11.225961333777482	0.002838285582618752
110-111	0.0	7.740778861687505E-5	0.0	11.964805774827452	0.002993101159852502
112-113	0.0	7.740778861687505E-5	0.0	12.70359861068501	0.0030705089484693773
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6375	0.0	42.096024	1
GTATCAA	8935	0.0	36.709362	1
TCAACGC	10960	0.0	29.376637	4
CAACGCA	11045	0.0	29.046991	5
GTACATG	16410	0.0	29.000336	1
TATCAAC	11330	0.0	28.574057	2
TACATGG	16960	0.0	27.736595	2
ATCAACG	11655	0.0	27.624878	3
AACGCAG	11695	0.0	27.519447	6
ACATGGG	17065	0.0	26.83328	3
CATGGGG	13120	0.0	25.58547	4
ACGCAGA	13665	0.0	23.377998	7
CGCAGAG	14575	0.0	21.755392	8
GTATACG	275	5.3918484E-8	21.68583	1
AGAGTAC	13620	0.0	19.634401	10-11
GCAGAGT	15960	0.0	19.420673	9
ATGGGGG	6515	0.0	18.91185	5
CAGAGTA	14740	0.0	18.667206	10-11
ATGGGGA	6060	0.0	16.304728	5
GTATAAT	1290	0.0	16.180319	1
>>END_MODULE