FastQCFastQC Report
Mon 15 Aug 2016
SRR2921124_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921124_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2949856
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA66650.044.0364841
GGTATCA43000.042.227681
TCAACGC81250.035.3727384
TATCAAC84450.034.3858572
CAACGCA83500.034.3477365
AACGCAG87000.033.1010366
ATCAACG88550.032.4577333
GTACATG139300.032.224491
TACATGG144700.030.020232
ACATGGG147650.029.0174143
ACGCAGA100450.028.4319577
CATGGGG103100.028.0493324
CGCAGAG108850.026.0738668
GCAGAGT112750.024.274869
AGAGTAC100150.021.09240210-11
CAGAGTA107450.020.65622910-11
ATGGGGG52450.019.9666295
GTGTACG4900.019.4397091
ATGGGGA51000.019.1342415
GTATATA8900.018.7298321
Produced by FastQC (version 0.11.5)