##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921124_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2949856
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.214061635551023	31.0	30.0	34.0	23.0	34.0
2	29.269631805755942	31.0	30.0	34.0	23.0	34.0
3	29.328017706627037	31.0	30.0	34.0	23.0	34.0
4	32.754779216341404	35.0	33.0	37.0	25.0	37.0
5	32.81985832528774	35.0	33.0	37.0	26.0	37.0
6	32.758762461625246	37.0	33.0	37.0	25.0	37.0
7	32.71841981439094	37.0	33.0	37.0	25.0	37.0
8	32.64490266643524	36.0	33.0	37.0	25.0	37.0
9	33.95676839818621	38.0	34.0	39.0	24.0	39.0
10-11	33.90859604672228	38.0	34.0	39.0	23.5	39.0
12-13	33.87274005917577	38.0	34.0	39.0	23.0	39.0
14-15	34.85152546429385	39.0	34.0	41.0	23.0	41.0
16-17	34.74003392029984	39.0	34.0	41.0	22.0	41.0
18-19	34.79258512957921	39.0	34.0	41.0	22.0	41.0
20-21	34.82172621307616	39.0	34.0	41.0	22.0	41.0
22-23	34.77746744247855	39.0	34.0	41.0	22.0	41.0
24-25	34.673513724059745	39.0	34.0	41.0	22.0	41.0
26-27	34.603225716780756	39.0	34.0	41.0	21.5	41.0
28-29	34.4546299887181	39.0	34.0	41.0	20.0	41.0
30-31	34.3464597932916	39.0	34.0	41.0	19.0	41.0
32-33	34.2418429916579	39.0	33.0	41.0	18.5	41.0
34-35	34.12928037843203	39.0	33.0	41.0	18.0	41.0
36-37	33.97341565147587	38.5	33.0	41.0	17.0	41.0
38-39	33.80126690929998	38.0	33.0	41.0	16.0	41.0
40-41	33.67656116095158	38.0	33.0	41.0	15.0	41.0
42-43	33.56336461847629	38.0	33.0	40.5	14.5	41.0
44-45	33.382089498606035	38.0	32.5	40.0	11.0	41.0
46-47	33.12439200421987	38.0	32.0	40.0	9.0	41.0
48-49	32.96280547253832	38.0	32.0	40.0	8.0	41.0
50-51	32.77244499392513	38.0	32.0	40.0	7.0	41.0
52-53	32.53366249064361	37.5	31.5	40.0	4.0	41.0
54-55	32.25572417772257	37.0	31.0	40.0	2.0	41.0
56-57	31.96362042757341	36.5	31.0	40.0	2.0	41.0
58-59	31.70841119024115	36.0	31.0	40.0	2.0	41.0
60-61	31.382722580356464	35.5	30.0	39.0	2.0	41.0
62-63	31.087857169977113	35.0	30.0	39.0	2.0	41.0
64-65	30.744799068157903	35.0	29.5	39.0	2.0	40.0
66-67	30.446989107264898	35.0	29.0	38.0	2.0	40.0
68-69	30.083763580323918	35.0	29.0	37.5	2.0	40.0
70-71	29.766452159020645	34.5	29.0	37.0	2.0	39.0
72-73	29.42316387647397	34.0	29.0	36.0	2.0	39.0
74-75	29.366684848345137	34.0	29.0	36.0	2.0	39.0
76-77	29.141843873056857	34.0	29.0	35.5	2.0	38.0
78-79	28.910015099042123	34.0	29.0	35.0	2.0	37.0
80-81	28.594123747057484	34.0	28.5	35.0	2.0	37.0
82-83	28.378803405996766	34.0	28.0	35.0	2.0	36.0
84-85	28.231667579705586	34.0	28.0	35.0	2.0	36.0
86-87	28.052298824078193	34.0	27.5	35.0	2.0	36.0
88-89	27.918016845568054	34.0	27.0	35.0	2.0	35.0
90-91	27.78665009410629	34.0	27.0	35.0	2.0	35.0
92-93	28.00563112233275	34.0	28.5	35.0	2.0	35.0
94-95	28.006694733573436	34.0	29.0	35.0	2.0	35.0
96-97	27.95599276710456	34.0	29.0	35.0	2.0	35.0
98-99	27.854124574216506	34.0	28.0	35.0	2.0	35.0
100-101	27.760127782508704	34.0	27.0	35.0	2.0	35.0
102-103	26.419563870236377	32.0	24.5	34.0	2.0	35.0
104-105	27.456763313192237	34.0	27.0	35.0	2.0	35.0
106-107	27.63756213862643	34.0	27.0	35.0	2.0	35.0
108-109	27.584691083225756	34.0	27.0	35.0	2.0	35.0
110-111	27.562311855222763	34.0	27.0	35.0	2.0	35.0
112-113	27.478545393402253	34.0	27.0	35.0	2.0	35.0
114-115	27.340105923814587	34.0	27.0	35.0	2.0	35.0
116-117	27.17239977137867	34.0	27.0	35.0	2.0	35.0
118-119	27.046667023746245	34.0	27.0	35.0	2.0	35.0
120-121	26.860364370328586	34.0	25.0	35.0	2.0	35.0
122-123	26.704959326828156	34.0	25.0	35.0	2.0	35.0
124-125	25.62688483776835	33.0	22.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	140486.0
3	20851.0
4	14039.0
5	12600.0
6	13990.0
7	15421.0
8	16534.0
9	17757.0
10	19324.0
11	20039.0
12	20261.0
13	20444.0
14	19938.0
15	17878.0
16	14485.0
17	12864.0
18	11721.0
19	11454.0
20	12005.0
21	12786.0
22	15620.0
23	20698.0
24	28822.0
25	36623.0
26	42987.0
27	49734.0
28	58651.0
29	68436.0
30	80704.0
31	96911.0
32	119205.0
33	151873.0
34	201475.0
35	291132.0
36	479414.0
37	609882.0
38	152747.0
39	65.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.9157818466514	18.187745232894713	12.861219764291478	25.03525315616241
2	16.789813141018254	19.761974827807393	37.57229784065929	25.875914190515065
3	19.567939473280013	23.86603099234431	28.435033426473794	28.130996107901886
4	13.932978267473906	15.831556647787687	34.40758387040774	35.82788121433067
5	15.100342459135277	36.69859358159502	32.89113013548851	15.309933823781193
6	33.92939539548159	34.34186075920614	16.760482251546915	14.968261593765353
7	29.659692999526712	31.39780958050863	20.160687720116684	18.78180969984798
8	27.71641199518446	33.41532137901294	19.334306793051436	19.533959832751165
9	27.18431395416247	15.715032042469707	18.580801504163674	38.51985249920415
10-11	26.189202791816175	25.39769208823246	26.369264187632723	22.04384093231865
12-13	26.517757266308735	22.985272527154738	26.447426256266265	24.049543950270262
14-15	24.11316969113841	24.203520741414277	23.767547258854233	27.915762308593077
16-17	24.349889090525355	27.032465919811223	24.506374434115873	24.111270555547552
18-19	24.387538769081964	26.24189466200785	25.85998837499638	23.510578193913805
20-21	25.418373335584594	25.162648472143534	25.52749504231115	23.89148314996072
22-23	25.271492146534737	24.826449452608504	25.365365558797677	24.536692842059086
24-25	24.72992935812085	25.285775429169544	25.35396793782498	24.630327274884632
26-27	24.441114601043136	25.488116809701143	25.46992885849842	24.6008397307573
28-29	24.48616479850635	25.562257686274847	25.554934558872265	24.396642956346533
30-31	24.689690547421048	25.41531249274285	25.34147388705168	24.553523072784422
32-33	24.493431273089705	25.362837303554763	25.240166826424748	24.90356459693078
34-35	24.74861639214877	25.43131322942523	25.30499210832019	24.515078270105814
36-37	24.759701606893795	25.259114082746997	25.368753614816054	24.612430695543157
38-39	24.64695661371249	25.40890283100853	25.368069754894755	24.57607080038423
40-41	24.74504000321375	25.315989030537565	25.47964277886946	24.45932818737922
42-43	24.668564634938868	25.24270116729117	25.360403224466843	24.728330973303127
44-45	24.618507905114893	25.280586914566044	25.33121748295392	24.769687697365143
46-47	24.73704604719848	25.332535979247094	25.28812552631472	24.642292447239704
48-49	24.665640715666083	25.6027772885705	25.08524778174398	24.646334214019436
50-51	24.530473498055922	25.726702844025905	25.170854794358938	24.57196886355924
52-53	24.790463375713585	25.447762383111904	25.102001103154432	24.65977313802008
54-55	24.5542553599028	25.38251845001249	25.24652281380616	24.81670337627855
56-57	24.5871503961451	25.46361570586239	25.115541614134912	24.8336922838576
58-59	24.524138805441144	25.348995951736796	25.194374434598338	24.932490808223722
60-61	24.518787684829515	25.43323304668948	25.404670931377527	24.643308337103477
62-63	24.516279944877525	25.44510720236491	25.239720048342342	24.79889280441522
64-65	24.461720876108885	25.332710681168475	25.24477095073392	24.96079749198872
66-67	24.520299196547874	25.412959748406138	25.187540245915574	24.879200809130413
68-69	24.57059113278783	25.284704681079567	25.26483900154959	24.87986518458301
70-71	24.359847726585862	25.241018494144825	25.44674252765121	24.952391251618103
72-73	24.551274966929984	25.30832577915399	25.2975114939918	24.842887759924224
74-75	24.499285710895638	25.431624531406683	25.26854490461342	24.800544853084258
76-77	24.579948234041797	25.19902095555145	25.34053830494455	24.880492505462207
78-79	24.48706316233207	25.30662984221409	25.36570061238676	24.84060638306708
80-81	24.48748691247678	25.345326705429073	25.366598962693548	24.800587419400603
82-83	24.553609490363172	25.266644416989237	25.371276660839825	24.80846943180776
84-85	24.479328751399226	25.321643909546466	25.317084349488074	24.881942989566237
86-87	24.479901608573044	25.246787930439186	25.37789626965841	24.89541419132936
88-89	24.42127191105318	25.166122712915307	25.357962813394906	25.05464256263661
90-91	24.400963780269624	25.19456547256337	25.40696664313434	24.99750410403267
92-93	24.391050077785174	25.17088498812289	25.5371821257845	24.900882808307436
94-95	24.350902612254888	25.082064134608572	25.588415863031965	24.97861739010457
96-97	24.431502486408572	24.91747849118443	25.605431757754747	25.04558726465225
98-99	24.351415683996542	25.04662559196238	25.475413231650585	25.12654549239049
100-101	24.19335345998172	25.11073131399012	25.65684928964322	25.03906593638494
102-103	24.09926199818665	25.020395523138177	25.737989100578197	25.14235337809698
104-105	24.040040234150005	25.186211768997396	25.57082285074615	25.20292514610644
106-107	24.09672782982959	25.16384412741931	25.57912667431383	25.16030136843727
108-109	24.00568901787311	25.162597250251522	25.613650252539166	25.2180634793362
110-111	24.042170663950806	25.297346814095267	25.5663167644544	25.094165757499525
112-113	23.992868612249097	25.353598823504086	25.63895942624806	25.01457313799876
114-115	23.952469848689574	25.487678344435444	25.549278570495048	25.010573236379933
116-117	23.956889200247677	25.45424774505478	25.554729320974733	25.034133733722808
118-119	24.07598347766673	25.443454197544906	25.43121618549979	25.04934613928858
120-121	23.952969326561423	25.424898410348458	25.569623299995303	25.052508963094816
122-123	24.01835220669895	25.396750870217872	25.455499898977436	25.12939702410575
124-125	24.04624693224917	25.509861163864844	25.39628631447663	25.04760558940936
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	598.0
1	516.5
2	370.0
3	272.5
4	209.5
5	187.0
6	220.0
7	253.0
8	291.5
9	349.0
10	356.5
11	357.5
12	399.5
13	447.0
14	506.5
15	590.5
16	658.5
17	720.0
18	817.0
19	932.5
20	1056.0
21	1243.0
22	1492.0
23	1790.0
24	2239.0
25	2814.5
26	3595.0
27	4821.0
28	6461.0
29	8688.0
30	11875.0
31	16222.5
32	21263.5
33	24988.5
34	28431.0
35	33206.0
36	39431.5
37	45933.0
38	52365.5
39	58571.5
40	64173.0
41	70580.5
42	77548.5
43	85334.0
44	93283.5
45	101141.5
46	110178.5
47	118192.0
48	124071.0
49	128743.0
50	131028.0
51	133062.5
52	133101.0
53	132499.5
54	131909.0
55	127205.0
56	119304.0
57	109360.0
58	100323.0
59	91262.0
60	80072.0
61	68560.0
62	57070.0
63	44095.0
64	32956.0
65	25477.0
66	19658.5
67	15269.5
68	11775.5
69	8861.0
70	6773.5
71	5226.5
72	3944.0
73	3234.0
74	2636.0
75	1999.0
76	1464.5
77	1018.5
78	684.5
79	445.5
80	283.0
81	195.0
82	153.0
83	98.5
84	61.5
85	46.5
86	43.0
87	36.5
88	27.5
89	22.0
90	15.0
91	16.0
92	12.5
93	11.5
94	12.5
95	6.0
96	7.0
97	7.0
98	6.5
99	7.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06417262401961316
2	0.008542789885336776
3	0.018543278044758794
4	0.018441578165171453
5	0.01040728767777139
6	0.009322488962173069
7	0.009627588600935096
8	0.008102090407124958
9	0.006000292895653211
10-11	0.010390337697840165
12-13	0.007881740668019048
14-15	0.00847498996561188
16-17	0.012831134807936387
18-19	0.006085042795309331
20-21	0.013458284065391665
22-23	0.004712094420880206
24-25	0.004017145243700031
26-27	0.0034238959461072
28-29	0.010051338099215691
30-31	0.006085042795309331
32-33	0.002830646648514368
34-35	0.007813940748294154
36-37	0.00644099237386503
38-39	0.0014068483342915721
40-41	0.0010339487758046495
42-43	0.0011864985951856634
44-45	0.0019322977121595088
46-47	0.0033899959862447523
48-49	0.0024577470900274453
50-51	0.004373094822255731
52-53	0.004508694661705521
54-55	0.004373094822255731
56-57	0.0017966978727097188
58-59	0.0024577470900274453
60-61	0.004898544200123667
62-63	0.002067897551609299
64-65	0.003678145645075556
66-67	7.627490969050692E-4
68-69	0.0015254981938101384
70-71	0.0010339487758046495
72-73	0.0018305978325721662
74-75	0.0023729971903713267
76-77	0.0013898983543603484
78-79	1.5254981938101384E-4
80-81	1.5254981938101384E-4
82-83	1.694997993122376E-5
84-85	3.389995986244752E-4
86-87	1.864497792434614E-4
88-89	1.1864985951856634E-4
90-91	4.5764945814304154E-4
92-93	0.0022034973910590888
94-95	9.83098836010978E-4
96-97	6.440992373865029E-4
98-99	9.322488962173069E-4
100-101	0.001203448575116887
102-103	0.0022543473308527604
104-105	0.004169695063081046
106-107	0.006017242875584436
108-109	0.010627637416877299
110-111	0.009152989162860831
112-113	0.002695046809064578
114-115	0.006491842313658701
116-117	0.002084847531540523
118-119	0.0010339487758046495
120-121	0.004118845123287374
122-123	6.779991972489504E-4
124-125	0.08341085124155213
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2949856.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.91395330021868
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.68522878282758	42.110890721967785
2	11.941563567783298	13.115169281856895
3	4.369436879366239	7.198291582253126
4	2.10674555554061	4.627589082096013
5	1.200668485357217	3.296672656697526
6	0.7585418611093633	2.499271940233233
7	0.5067598518177238	1.9479730786000955
8	0.39446226327743017	1.7329185843452295
9	0.3025901163176178	1.4954777564916062
>10	1.6200385477560881	16.11820275143449
>50	0.08773562404838305	3.2721318673618187
>100	0.02523590139673395	2.204280426485468
>500	9.305281892285559E-4	0.34086427274823833
>1k	6.203521259125082E-5	0.040265997428364106
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.779991972489504E-5
2	0.0	0.0	0.0	0.0	6.779991972489504E-5
3	0.0	0.0	0.0	0.0	6.779991972489504E-5
4	0.0	0.0	0.0	0.0	1.0169987958734257E-4
5	0.0	0.0	0.0	0.0	1.0169987958734257E-4
6	0.0	0.0	0.0	0.0	1.0169987958734257E-4
7	0.0	0.0	0.0	0.0	1.0169987958734257E-4
8	0.0	0.0	0.0	0.0	1.0169987958734257E-4
9	0.0	0.0	0.0	3.389995986244752E-5	1.0169987958734257E-4
10-11	0.0	0.0	0.0	3.389995986244752E-5	1.355998394497901E-4
12-13	0.0	0.0	0.0	5.0849939793671284E-5	2.711996788995802E-4
14-15	0.0	0.0	0.0	6.779991972489504E-5	3.898495384181465E-4
16-17	6.779991972489504E-5	0.0	0.0	6.779991972489504E-5	4.406994782118178E-4
18-19	6.779991972489504E-5	0.0	0.0	6.779991972489504E-5	5.084993979367128E-4
20-21	6.779991972489504E-5	0.0	0.0	8.47498996561188E-5	6.610492173177267E-4
22-23	6.779991972489504E-5	0.0	0.0	2.3729971903713268E-4	7.79699076836293E-4
24-25	6.779991972489504E-5	0.0	0.0	5.762993176616079E-4	8.135990366987405E-4
26-27	6.779991972489504E-5	0.0	0.0	0.0012373485349793346	8.474989965611881E-4
28-29	6.779991972489504E-5	0.0	0.0	0.004644294501155311	8.474989965611881E-4
30-31	6.779991972489504E-5	0.0	0.0	0.015017682219064254	8.644489764924119E-4
32-33	6.779991972489504E-5	0.0	0.0	0.03886630398229608	8.983489363548593E-4
34-35	6.779991972489504E-5	0.0	0.0	0.07180011498866384	9.491988761485306E-4
36-37	6.779991972489504E-5	0.0	0.0	0.12424335289587017	0.0010000488159422017
38-39	6.779991972489504E-5	0.0	0.0	0.20501000726815138	0.0010678487356670968
40-41	6.779991972489504E-5	0.0	0.0	0.29392960198735124	0.0010847987155983207
42-43	6.779991972489504E-5	0.0	0.0	0.3811541987134287	0.0010847987155983207
44-45	6.779991972489504E-5	0.0	0.0	0.48666782378529666	0.0010847987155983207
46-47	6.779991972489504E-5	0.0	0.0	0.6067245316381545	0.0010847987155983207
48-49	6.779991972489504E-5	0.0	0.0	0.7292559365609711	0.0011186986754607683
50-51	6.779991972489504E-5	0.0	0.0	0.8511093422865388	0.0011186986754607683
52-53	6.779991972489504E-5	0.0	0.0	0.9784036915700292	0.0012373485349793346
54-55	6.779991972489504E-5	0.0	0.0	1.1249193180955275	0.0013220984346354533
56-57	6.779991972489504E-5	0.0	0.0	1.288588324311424	0.0014068483342915723
58-59	6.779991972489504E-5	0.0	0.0	1.4638341668203463	0.0014576982740852435
60-61	6.779991972489504E-5	0.0	0.0	1.6418428560580585	0.0014576982740852435
62-63	6.779991972489504E-5	0.0	0.0	1.823377141121465	0.001491598233947691
64-65	6.779991972489504E-5	0.0	0.0	2.0303702960415695	0.0015085482138789148
66-67	6.779991972489504E-5	0.0	0.0	2.267432715359665	0.001559398153672586
68-69	6.779991972489504E-5	0.0	0.0	2.520784065391666	0.001559398153672586
70-71	6.779991972489504E-5	0.0	0.0	2.784254553442609	0.0015763481336038099
72-73	6.779991972489504E-5	0.0	0.0	3.063844472408145	0.0017458479329160474
74-75	8.47498996561188E-5	0.0	0.0	3.3633506177928685	0.001779747892778495
76-77	1.0169987958734257E-4	0.0	0.0	3.690875080003905	0.0018475478125033898
78-79	1.0169987958734257E-4	0.0	0.0	4.055858997862947	0.0018644977924346137
80-81	1.0169987958734257E-4	0.0	0.0	4.427317808055715	0.001915347732228285
82-83	1.0169987958734257E-4	0.0	0.0	4.817133446513999	0.0020339975917468512
84-85	1.0169987958734257E-4	0.0	0.0	5.2290688087825306	0.0020339975917468512
86-87	1.0169987958734257E-4	0.0	0.0	5.677836477441611	0.0020848475315405225
88-89	1.0169987958734257E-4	0.0	0.0	6.163114402872547	0.0021695974311966414
90-91	1.0169987958734257E-4	0.0	0.0	6.670901901652149	0.0021695974311966414
92-93	1.0169987958734257E-4	0.0	0.0	7.188571238731653	0.0022373973509215365
94-95	1.0169987958734257E-4	0.0	0.0	7.725343203193647	0.0023051972706464316
96-97	1.0169987958734257E-4	0.0	0.0	8.305235916600676	0.0023729971903713263
98-99	1.0169987958734257E-4	0.0	0.0	8.92097783756224	0.002406897150233774
100-101	1.0169987958734257E-4	0.0	0.0	9.562466778039335	0.002406897150233774
102-103	1.1864985951856633E-4	0.0	0.0	10.206667715305425	0.002440797110096222
104-105	1.355998394497901E-4	0.0	0.0	10.867072833385766	0.002440797110096222
106-107	1.355998394497901E-4	0.0	0.0	11.555970867730494	0.002491647049889893
108-109	1.355998394497901E-4	0.0	0.0	12.28351485631841	0.0025085970298211165
110-111	1.355998394497901E-4	0.0	0.0	13.034415917251554	0.0025085970298211165
112-113	1.355998394497901E-4	0.0	0.0	13.791452870919802	0.0025763969495460116
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6665	0.0	44.036484	1
GGTATCA	4300	0.0	42.22768	1
TCAACGC	8125	0.0	35.372738	4
TATCAAC	8445	0.0	34.385857	2
CAACGCA	8350	0.0	34.347736	5
AACGCAG	8700	0.0	33.101036	6
ATCAACG	8855	0.0	32.457733	3
GTACATG	13930	0.0	32.22449	1
TACATGG	14470	0.0	30.02023	2
ACATGGG	14765	0.0	29.017414	3
ACGCAGA	10045	0.0	28.431957	7
CATGGGG	10310	0.0	28.049332	4
CGCAGAG	10885	0.0	26.073866	8
GCAGAGT	11275	0.0	24.27486	9
AGAGTAC	10015	0.0	21.092402	10-11
CAGAGTA	10745	0.0	20.656229	10-11
ATGGGGG	5245	0.0	19.966629	5
GTGTACG	490	0.0	19.439709	1
ATGGGGA	5100	0.0	19.134241	5
GTATATA	890	0.0	18.729832	1
>>END_MODULE