FastQCFastQC Report
Mon 15 Aug 2016
SRR2921124_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921124_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2949856
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32820.11125966826855277No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48900.053.316471
GTATCAA79750.046.382041
TCAACGC102050.035.4763574
TATCAAC106400.034.5281142
CAACGCA106100.034.1105545
ATCAACG108000.033.8526543
AACGCAG110750.032.6589786
GTACATG135150.029.311691
ACGCAGA127600.028.1597677
TACATGG142900.027.2112942
CGCAGAG135000.026.7047798
ACATGGG142100.026.568413
CATGGGG99850.024.7295154
GCAGAGT142900.024.3125659
AGAGTAC127650.022.5100510-11
GAGTACT60500.022.2723812-13
CAGAGTA136100.021.59329610-11
GTACTTT65550.020.82895514-15
AGTACTT64550.019.5846812-13
CATGGGT23700.019.3778554
Produced by FastQC (version 0.11.5)