FastQCFastQC Report
Mon 15 Aug 2016
SRR2921123_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921123_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2585143
Sequences flagged as poor quality0
Sequence length125
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40250.043.3376661
GGTATCA25500.036.8876231
TCAACGC49800.033.576134
GTACATG99450.032.565511
CAACGCA52350.031.8275665
TACATGG102400.031.2046722
ATCAACG55050.030.589643
TATCAAC55450.030.1543562
AACGCAG56200.029.8583986
ACATGGG106500.029.3328973
CATGGGG84300.027.8819524
ACGCAGA67950.024.432537
CGCAGAG73950.022.4501748
GTATAGG7100.021.8011191
AGAGTAC67650.019.74838610-11
ATGGGGA41050.019.7145945
ATGGGGG46500.019.3235135
GCAGAGT81300.018.7367699
GTATATA8550.017.407551
TACACCG4602.4847395E-916.8169635
Produced by FastQC (version 0.11.5)