##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921123_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2585143
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.67144796245314	31.0	28.0	33.0	10.0	34.0
2	27.780144077136157	31.0	28.0	34.0	10.0	34.0
3	27.827865615171	31.0	28.0	34.0	10.0	34.0
4	31.136505021192253	35.0	32.0	37.0	10.0	37.0
5	31.17807873684357	35.0	32.0	37.0	10.0	37.0
6	31.10038400196817	35.0	32.0	37.0	10.0	37.0
7	31.018471705433704	35.0	32.0	37.0	10.0	37.0
8	30.924163189425112	35.0	32.0	37.0	10.0	37.0
9	32.08197573596509	37.0	32.0	39.0	10.0	39.0
10-11	32.02073676388501	37.0	32.0	39.0	9.0	39.0
12-13	31.96489536555618	37.0	32.0	39.0	8.0	39.0
14-15	32.80360525510581	38.0	32.0	40.5	7.5	41.0
16-17	32.66291574586009	38.0	31.0	40.0	7.0	41.0
18-19	32.67931735304391	38.0	31.5	40.0	2.0	41.0
20-21	32.69869055599632	38.0	32.0	40.0	2.0	41.0
22-23	32.62977077090126	38.0	31.0	40.0	2.0	41.0
24-25	32.51559855683031	38.0	31.0	40.0	2.0	41.0
26-27	32.42518421611493	38.0	31.0	40.0	2.0	41.0
28-29	32.260160656489795	38.0	30.5	40.0	2.0	41.0
30-31	32.154154721808425	38.0	30.0	40.0	2.0	41.0
32-33	32.046040006297524	38.0	30.0	40.0	2.0	41.0
34-35	31.92968628814731	38.0	30.0	40.0	2.0	41.0
36-37	31.76022332226883	38.0	30.0	40.0	2.0	41.0
38-39	31.590700978630583	38.0	30.0	40.0	2.0	41.0
40-41	31.463664872697564	38.0	30.0	40.0	2.0	41.0
42-43	31.339915045318577	37.5	30.0	40.0	2.0	41.0
44-45	31.147736121367366	37.0	29.5	40.0	2.0	41.0
46-47	30.889274790601526	37.0	29.0	40.0	2.0	41.0
48-49	30.72846511779039	37.0	29.0	40.0	2.0	41.0
50-51	30.535364774791958	36.5	28.0	40.0	2.0	41.0
52-53	30.303723817212433	36.0	28.0	40.0	2.0	41.0
54-55	30.034782988794042	36.0	28.0	40.0	2.0	41.0
56-57	29.747852826710165	35.5	27.0	39.5	2.0	41.0
58-59	29.496978890529462	35.0	27.0	39.0	2.0	41.0
60-61	29.171242751368105	35.0	26.0	39.0	2.0	40.5
62-63	28.88003255525903	35.0	26.0	38.5	2.0	40.0
64-65	28.553491431615193	34.0	26.0	38.0	2.0	40.0
66-67	28.2712171048178	34.0	26.0	37.5	2.0	40.0
68-69	27.924495859610087	34.0	25.5	37.0	2.0	39.5
70-71	27.62705583404864	34.0	25.0	36.5	2.0	39.0
72-73	27.29013559404644	34.0	24.5	36.0	2.0	39.0
74-75	27.238684861920596	34.0	25.0	36.0	2.0	39.0
76-77	27.02452456208419	34.0	24.5	35.0	2.0	37.5
78-79	26.79541209132338	33.0	24.0	35.0	2.0	37.0
80-81	26.48934352954556	33.0	23.5	35.0	2.0	36.5
82-83	26.27232033198937	33.0	23.0	35.0	2.0	36.0
84-85	26.12790027476236	33.0	23.0	35.0	2.0	36.0
86-87	25.947529981900423	33.0	23.0	35.0	2.0	35.0
88-89	25.814749899715412	33.0	21.5	35.0	2.0	35.0
90-91	25.686820419605414	33.0	20.0	35.0	2.0	35.0
92-93	25.876592900276698	33.0	23.0	35.0	2.0	35.0
94-95	25.87716308150071	33.0	23.0	35.0	2.0	35.0
96-97	25.826934138653066	33.0	23.0	35.0	2.0	35.0
98-99	25.733466195100235	33.0	20.0	35.0	2.0	35.0
100-101	25.62592359494233	33.0	20.0	35.0	2.0	35.0
102-103	24.36453341265841	31.0	18.5	34.0	2.0	35.0
104-105	25.34414034349357	33.0	19.5	35.0	2.0	35.0
106-107	25.528607508366076	33.0	19.0	35.0	2.0	35.0
108-109	25.48327597351481	33.0	19.0	35.0	2.0	35.0
110-111	25.45183535301529	33.0	19.0	35.0	2.0	35.0
112-113	25.374634014443302	33.0	18.0	35.0	2.0	35.0
114-115	25.27164029223915	33.0	18.0	35.0	2.0	35.0
116-117	25.113211532205376	33.0	16.0	35.0	2.0	35.0
118-119	24.98869443585906	33.0	8.5	35.0	2.0	35.0
120-121	24.800908885891417	33.0	2.0	35.0	2.0	35.0
122-123	24.6304817567152	33.0	2.0	35.0	2.0	35.0
124-125	23.60080660915083	32.0	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	215728.0
3	28416.0
4	18015.0
5	15181.0
6	16013.0
7	17823.0
8	19399.0
9	20401.0
10	21282.0
11	21523.0
12	21518.0
13	21138.0
14	19608.0
15	17036.0
16	14778.0
17	13282.0
18	12205.0
19	11502.0
20	11522.0
21	12689.0
22	15469.0
23	20511.0
24	27653.0
25	35207.0
26	41806.0
27	48370.0
28	55767.0
29	64089.0
30	74165.0
31	88567.0
32	106048.0
33	132846.0
34	171929.0
35	240492.0
36	377520.0
37	439247.0
38	96371.0
39	27.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.07041488096713	18.23991441028563	13.011827420913122	25.677843287834122
2	16.288935621693305	19.059285668967714	38.67495477595334	25.976823933385635
3	19.501347766252024	23.410624352184264	28.12237028886343	28.96565759270028
4	13.75604815152704	15.369713936844647	34.43699282772218	36.437245083906134
5	15.127204836112696	36.48408479136024	33.2622018920757	15.126508480451362
6	34.66551742947414	34.246391450665655	16.47086950651533	14.617221613344872
7	29.960876342083058	30.97438528117929	20.121545247150642	18.94319312958701
8	27.535192415119624	34.39297436064082	18.925604232529132	19.14622899171042
9	27.119667585955575	15.61059339589759	18.399408271433813	38.870330746713016
10-11	26.3008807652423	25.20259844412906	26.504199752829415	21.992321037799226
12-13	26.433539786377192	22.606337502369502	26.716526172855744	24.243596538397558
14-15	23.99035013265473	23.980195024499924	23.709779001634875	28.319675841210472
16-17	24.44835620585365	26.903190675478445	24.452379731874547	24.19607338679336
18-19	24.445914487955797	26.33385655276731	25.682569249204292	23.537659710072603
20-21	25.255496027508766	25.35852236325314	25.441082708907853	23.94489890033024
22-23	25.019762991108152	24.863902768598248	25.394133133411362	24.722201106882242
24-25	24.68050109389679	25.281617253530865	25.370494554306934	24.667387098265415
26-27	24.46461218038237	25.493647685414782	25.402237178351456	24.639502955851395
28-29	24.478610641977664	25.638020951711482	25.470370894077544	24.412997512233307
30-31	24.739995574392758	25.441168050829493	25.325498770578687	24.493337604199066
32-33	24.561886716836963	25.371306374856772	25.242644083584842	24.82416282472142
34-35	24.686988198697332	25.425751950780807	25.38611832870296	24.501141521818898
36-37	24.760287167964943	25.360159197328553	25.340449088731464	24.53910454597504
38-39	24.680484888528014	25.51567857044767	25.2931136993736	24.51072284165071
40-41	24.73902444307936	25.444700956401046	25.399480408555462	24.41679419196413
42-43	24.611341039268396	25.351194360620276	25.366822340429234	24.670642259682094
44-45	24.62331414693983	25.370776941589973	25.290914763323435	24.714994148146758
46-47	24.670241217376873	25.361663591593686	25.331025690769575	24.637069500259862
48-49	24.640415648640577	25.597005572000427	25.18626202482875	24.576316754530247
50-51	24.50186391820694	25.751510196376305	25.184568811396	24.562057074020753
52-53	24.725154207261525	25.47308602144298	25.154903587821252	24.64685618347424
54-55	24.56313447628361	25.472107221414575	25.247078730184764	24.717679572117046
56-57	24.595338308141123	25.518245679118127	25.190365602146	24.69605041059475
58-59	24.60901369984894	25.440598205460986	25.222712001439035	24.72767609325104
60-61	24.684338195798812	25.479152752286215	25.31971401995083	24.516795031964147
62-63	24.548627115041263	25.565324628527758	25.207657541694463	24.678390714736516
64-65	24.517533509990137	25.412197056149783	25.2481963598383	24.82207307402178
66-67	24.52859200782321	25.4562830157733	25.165639767160464	24.849485209243024
68-69	24.55833125895392	25.45457921431212	25.210140920475162	24.776948606258802
70-71	24.428311120678668	25.293646285257722	25.352134741620862	24.92590785244275
72-73	24.53927118176589	25.370112982570426	25.30769733653271	24.782918499130975
74-75	24.353324347173682	25.53632151187204	25.327547066606865	24.782807074347417
76-77	24.491954675711305	25.28561713571292	25.34530525657768	24.877122931998095
78-79	24.392799312378198	25.334430371716525	25.407849936289768	24.86492037961551
80-81	24.425843696726794	25.464819907308733	25.33854052835029	24.770795867614183
82-83	24.488316507055895	25.254250925384014	25.496403873982988	24.761028693577106
84-85	24.36524640667013	25.369796649075077	25.335736558642736	24.92922038561206
86-87	24.348071805810054	25.379196244379898	25.475148531665027	24.79758341814502
88-89	24.27610387510478	25.206613328547007	25.498338002965404	25.0189447933828
90-91	24.272586203928654	25.310056401320708	25.4370710692229	24.980286325527743
92-93	24.244269316727898	25.313706736399688	25.624101218619415	24.817922728253
94-95	24.212232232069603	25.199226651286345	25.628877719843672	24.959663396800384
96-97	24.22817197903857	25.106387340825158	25.67245193566743	24.992988744468843
98-99	24.15169544861819	25.174817330057675	25.55814472557828	25.115342495745857
100-101	24.105601409916844	25.15452878921921	25.740072886449628	24.99979691441432
102-103	23.883947740391644	25.108409984317788	25.84586866086881	25.161773614421755
104-105	23.865653428537332	25.29769785483466	25.652430489134524	25.18421822749348
106-107	23.87025956404382	25.111609927630774	25.781395218389115	25.236735289936295
108-109	23.786949284830712	25.142951648579093	25.777256369015554	25.292842697574642
110-111	23.7252256155684	25.282599747459116	25.791820894044243	25.20035374292824
112-113	23.76761401066966	25.308413217814334	25.81257419963042	25.11139857188559
114-115	23.630972747984867	25.44808968195957	25.779269373731506	25.141668196324062
116-117	23.545692869551896	25.33129317778018	25.822067798616455	25.300946154051474
118-119	23.684249209224635	25.235008341969294	25.772043165484327	25.30869928332175
120-121	23.507120591650875	25.268777480253675	25.920257265076955	25.303844663018495
122-123	23.53793449992979	25.27241408255765	25.831206745355257	25.358444672157304
124-125	23.49922424648346	25.354315618923547	25.730916608674697	25.4155435259183
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	414.0
1	314.0
2	192.5
3	151.5
4	142.5
5	140.5
6	116.5
7	134.0
8	163.5
9	171.0
10	203.5
11	234.5
12	243.0
13	267.0
14	288.5
15	320.5
16	409.5
17	476.0
18	522.5
19	588.0
20	679.5
21	820.5
22	1012.0
23	1245.5
24	1531.5
25	1933.0
26	2626.5
27	3609.5
28	4947.0
29	6878.5
30	9560.0
31	12668.0
32	16236.0
33	20041.0
34	23936.0
35	28535.0
36	34013.5
37	40134.0
38	45982.0
39	51549.5
40	57485.5
41	63918.0
42	70727.0
43	77903.0
44	85338.0
45	92590.5
46	99652.5
47	105854.5
48	111774.0
49	116037.5
50	117215.5
51	118740.5
52	118950.5
53	117883.0
54	116721.0
55	111816.0
56	104233.5
57	95152.0
58	86548.5
59	78314.5
60	68654.5
61	58595.0
62	48375.5
63	37574.5
64	27776.0
65	20908.0
66	15908.0
67	12045.0
68	8956.0
69	6675.0
70	5117.5
71	3840.5
72	2822.5
73	2116.0
74	1583.5
75	1195.5
76	877.5
77	581.5
78	384.5
79	259.0
80	183.0
81	158.5
82	116.0
83	76.5
84	55.0
85	40.5
86	33.5
87	26.5
88	18.5
89	12.0
90	8.0
91	9.5
92	8.5
93	5.5
94	6.0
95	5.5
96	6.0
97	8.0
98	9.0
99	12.0
100	13.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06401966931809962
2	0.009090406217373661
3	0.019186559505605685
4	0.018142129855098922
5	0.00994142296963843
6	0.009283819115615655
7	0.010134835867880424
8	0.008162024305812096
9	0.006730768858821349
10-11	0.011159924228562984
12-13	0.008239389465108894
14-15	0.009167771376670459
16-17	0.013364831268521704
18-19	0.007175618524777933
20-21	0.014196506730962273
22-23	0.005202806962709606
24-25	0.004583885688335229
26-27	0.003906940544488254
28-29	0.011392019706453376
30-31	0.007233642394250531
32-33	0.0031332889515202833
34-35	0.008374778493878288
36-37	0.0067694514384697485
38-39	0.0020888593010135225
40-41	0.0013538902876939496
42-43	0.0013925728673423482
44-45	0.0024950263873217073
46-47	0.004235742471499642
48-49	0.002901193473629892
50-51	0.00508675922376441
52-53	0.005725021787962987
54-55	0.005299513411830603
56-57	0.0019921528518925258
58-59	0.0030172412125750877
60-61	0.0053188547016548016
62-63	0.002224248329782917
64-65	0.003597479907301066
66-67	5.802386947259784E-4
68-69	0.001257183838572953
70-71	6.769451438469748E-4
72-73	0.0020888593010135225
74-75	0.0021855657501345183
76-77	0.0015086206062875438
78-79	3.868257964839856E-5
80-81	7.736515929679712E-5
82-83	0.0
84-85	2.3209547789039138E-4
86-87	1.5473031859359425E-4
88-89	0.0
90-91	3.288019270113878E-4
92-93	0.0017600573740021344
94-95	8.510167522647684E-4
96-97	5.415561150775798E-4
98-99	6.18921274374377E-4
100-101	0.0010057470708583626
102-103	0.0018954464027715295
104-105	0.0037715515157188597
106-107	0.005241489542358005
108-109	0.010270224896649818
110-111	0.00831675462440569
112-113	0.0021662244603103194
114-115	0.005473585020248396
116-117	0.001779398663826334
118-119	8.510167522647684E-4
120-121	0.004061670863081849
122-123	6.18921274374377E-4
124-125	0.08396053912684907
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2585143.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.947592479376546
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.9432515088063	51.58130402411397
2	9.694574290175408	12.205002233580057
3	3.2016879733566492	6.046156493789261
4	1.5092468533959782	3.8001382351340562
5	0.8851124990123674	2.785785044311654
6	0.5541615592587222	2.092988159997235
7	0.4065759000369407	1.7915081847222756
8	0.30030082833190413	1.5122571330444734
9	0.22209983194261523	1.2582584739775586
>10	1.2193693338148581	13.584311286602452
>50	0.05171901455551574	2.209634592102624
>100	0.011777063254381168	1.0860708694556596
>500	1.23344058482556E-4	0.0465852691686454
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.736515929679712E-5
2	0.0	0.0	0.0	3.868257964839856E-5	7.736515929679712E-5
3	0.0	0.0	0.0	3.868257964839856E-5	7.736515929679712E-5
4	0.0	0.0	0.0	3.868257964839856E-5	7.736515929679712E-5
5	0.0	0.0	0.0	3.868257964839856E-5	7.736515929679712E-5
6	0.0	0.0	0.0	3.868257964839856E-5	7.736515929679712E-5
7	0.0	0.0	0.0	3.868257964839856E-5	7.736515929679712E-5
8	0.0	0.0	0.0	3.868257964839856E-5	7.736515929679712E-5
9	0.0	0.0	0.0	7.736515929679712E-5	7.736515929679712E-5
10-11	0.0	0.0	0.0	7.736515929679712E-5	7.736515929679712E-5
12-13	0.0	0.0	0.0	7.736515929679712E-5	1.7407160841779353E-4
14-15	0.0	0.0	0.0	7.736515929679712E-5	3.094606371871885E-4
16-17	0.0	0.0	0.0	1.1604773894519568E-4	3.8682579648398563E-4
18-19	0.0	0.0	0.0	1.5473031859359425E-4	4.2550837613238414E-4
20-21	0.0	0.0	0.0	1.5473031859359425E-4	4.641909557807827E-4
22-23	0.0	0.0	0.0	1.5473031859359425E-4	5.028735354291813E-4
24-25	0.0	0.0	0.0	2.5143676771459064E-4	5.415561150775798E-4
26-27	0.0	0.0	3.868257964839856E-5	5.222148252533806E-4	5.608974049017791E-4
28-29	0.0	0.0	3.868257964839856E-5	0.002746463155036298	6.18921274374377E-4
30-31	0.0	0.0	3.868257964839856E-5	0.011140582938738784	6.382625641985762E-4
32-33	0.0	0.0	3.868257964839856E-5	0.026265471581262623	6.962864336711741E-4
34-35	0.0	0.0	3.868257964839856E-5	0.044040116929701764	7.349690133195727E-4
36-37	0.0	0.0	3.868257964839856E-5	0.0723364239425053	7.349690133195727E-4
38-39	0.0	0.0	3.868257964839856E-5	0.12486736710503055	7.54310303143772E-4
40-41	0.0	0.0	3.868257964839856E-5	0.18221429143378143	7.736515929679713E-4
42-43	0.0	0.0	3.868257964839856E-5	0.23611846617382481	7.736515929679713E-4
44-45	0.0	0.0	3.868257964839856E-5	0.2983587368280981	7.736515929679713E-4
46-47	0.0	0.0	3.868257964839856E-5	0.3703857001334162	7.929928827921705E-4
48-49	0.0	0.0	3.868257964839856E-5	0.44707391428636634	8.123341726163698E-4
50-51	0.0	0.0	3.868257964839856E-5	0.5270888302890788	8.896993319131669E-4
52-53	0.0	0.0	3.868257964839856E-5	0.6071230875816154	9.67064491209964E-4
54-55	0.0	0.0	3.868257964839856E-5	0.6964798465694161	0.0010057470708583626
56-57	0.0	0.0	3.868257964839856E-5	0.8000524535780031	0.0010057470708583626
58-59	0.0	0.0	3.868257964839856E-5	0.9133730706579868	0.001025088360682562
60-61	0.0	0.0	3.868257964839856E-5	1.0333277501476708	0.0010444296505067612
62-63	0.0	0.0	3.868257964839856E-5	1.1566091314871167	0.0011604773894519568
64-65	0.0	0.0	3.868257964839856E-5	1.2869887661920443	0.0011604773894519568
66-67	0.0	0.0	3.868257964839856E-5	1.4369031036194129	0.0011798186792761562
68-69	0.0	0.0	3.868257964839856E-5	1.5999888594170613	0.001237842548748754
70-71	0.0	0.0	3.868257964839856E-5	1.7779287257996947	0.001237842548748754
72-73	0.0	0.0	3.868257964839856E-5	1.9611487643043342	0.0013925728673423482
74-75	0.0	0.0	3.868257964839856E-5	2.153265796128106	0.0014699380266391454
76-77	0.0	0.0	3.868257964839856E-5	2.364859506804846	0.0014699380266391454
78-79	0.0	0.0	3.868257964839856E-5	2.6069544315343482	0.0014699380266391454
80-81	0.0	0.0	3.868257964839856E-5	2.858468564408236	0.0014892793164633447
82-83	0.0	0.0	3.868257964839856E-5	3.1189183731809034	0.0015086206062875438
84-85	0.0	0.0	3.868257964839856E-5	3.399966655616343	0.0015086206062875438
86-87	0.0	0.0	3.868257964839856E-5	3.6943797693203044	0.0015473031859359423
88-89	0.0	0.0	3.868257964839856E-5	4.013530392709417	0.001585985765584341
90-91	0.0	0.0	3.868257964839856E-5	4.359546067664342	0.0016246683452327397
92-93	0.0	0.0	3.868257964839856E-5	4.715812626226093	0.0016633509248811381
94-95	0.0	0.0	3.868257964839856E-5	5.080744082629085	0.0017020335045295366
96-97	0.0	0.0	3.868257964839856E-5	5.462753897946845	0.0017020335045295366
98-99	0.0	0.0	3.868257964839856E-5	5.883852459999312	0.0017020335045295366
100-101	0.0	0.0	3.868257964839856E-5	6.333440741962824	0.0017020335045295366
102-103	0.0	0.0	3.868257964839856E-5	6.798966246741476	0.0017020335045295366
104-105	0.0	0.0	3.868257964839856E-5	7.271880124232973	0.0017020335045295366
106-107	0.0	0.0	3.868257964839856E-5	7.757888054935453	0.0017020335045295366
108-109	0.0	0.0	3.868257964839856E-5	8.271766784274604	0.0017407160841779352
110-111	0.0	0.0	3.868257964839856E-5	8.818061515359112	0.001798739953650533
112-113	0.0	0.0	3.868257964839856E-5	9.37946179379632	0.0018567638231231308
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4025	0.0	43.337666	1
GGTATCA	2550	0.0	36.887623	1
TCAACGC	4980	0.0	33.57613	4
GTACATG	9945	0.0	32.56551	1
CAACGCA	5235	0.0	31.827566	5
TACATGG	10240	0.0	31.204672	2
ATCAACG	5505	0.0	30.58964	3
TATCAAC	5545	0.0	30.154356	2
AACGCAG	5620	0.0	29.858398	6
ACATGGG	10650	0.0	29.332897	3
CATGGGG	8430	0.0	27.881952	4
ACGCAGA	6795	0.0	24.43253	7
CGCAGAG	7395	0.0	22.450174	8
GTATAGG	710	0.0	21.801119	1
AGAGTAC	6765	0.0	19.748386	10-11
ATGGGGA	4105	0.0	19.714594	5
ATGGGGG	4650	0.0	19.323513	5
GCAGAGT	8130	0.0	18.736769	9
GTATATA	855	0.0	17.40755	1
TACACCG	460	2.4847395E-9	16.816963	5
>>END_MODULE