FastQCFastQC Report
Mon 15 Aug 2016
SRR2921123_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921123_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2585143
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32400.047.1446461
GTATCAA51000.045.5110441
TCAACGC67500.033.843274
CAACGCA69700.032.8773275
TATCAAC70750.032.4553532
ATCAACG72700.031.5040023
AACGCAG73550.031.3815126
GTACATG100450.027.4428651
ACGCAGA85700.026.4465477
TACATGG103450.026.0014822
ACATGGG103000.025.8844663
CATGGGG80350.025.1646654
CGCAGAG90750.025.041878
AGAGTAC88050.022.56730710-11
GCAGAGT103200.021.3294899
CAGAGTA97450.019.4134949
ACCGTGC6950.018.8316298
GTATACG2551.4618216E-518.7191961
GTACGTA3604.559297E-818.2317181
CTAATAC7150.017.5179043
Produced by FastQC (version 0.11.5)