##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921123_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2585143
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.767374570768425	31.0	31.0	34.0	27.0	34.0
2	30.836739398942342	31.0	31.0	34.0	27.0	34.0
3	30.932594444485275	31.0	31.0	34.0	27.0	34.0
4	34.470563910777855	37.0	35.0	37.0	31.0	37.0
5	34.1471601377564	37.0	35.0	37.0	30.0	37.0
6	34.048589188296354	37.0	35.0	37.0	30.0	37.0
7	33.874206958763985	37.0	35.0	37.0	29.0	37.0
8	33.85745469399565	37.0	35.0	37.0	29.0	37.0
9	35.292229868908606	39.0	35.0	39.0	29.0	39.0
10-11	35.25286512196811	39.0	35.0	39.0	28.5	39.0
12-13	35.14707890433914	39.0	35.0	39.0	28.0	39.0
14-15	36.261357688917016	40.0	36.0	41.0	27.5	41.0
16-17	36.2106519832752	40.0	36.0	41.0	27.0	41.0
18-19	36.23574808047369	40.0	36.0	41.0	27.0	41.0
20-21	36.21747597715097	40.0	36.0	41.0	27.0	41.0
22-23	36.19177140297461	40.0	36.0	41.0	27.0	41.0
24-25	36.11419581044453	40.0	36.0	41.0	27.0	41.0
26-27	36.01194053868586	40.0	36.0	41.0	27.0	41.0
28-29	35.9152977224084	39.5	35.0	41.0	27.0	41.0
30-31	35.84042546195704	39.0	35.0	41.0	27.0	41.0
32-33	35.73341319996611	39.0	35.0	41.0	27.0	41.0
34-35	35.63343826627772	39.0	35.0	41.0	27.0	41.0
36-37	35.512440317614924	39.0	35.0	41.0	26.0	41.0
38-39	35.359953782053836	39.0	35.0	41.0	25.0	41.0
40-41	35.21569561142266	39.0	35.0	41.0	25.0	41.0
42-43	35.09211250596195	39.0	34.0	41.0	25.0	41.0
44-45	34.93375376139734	39.0	34.0	41.0	24.0	41.0
46-47	34.746945333391615	39.0	34.0	41.0	24.0	41.0
48-49	34.56303693838213	38.0	34.0	41.0	23.0	41.0
50-51	34.34790183753858	38.0	33.0	40.5	23.0	41.0
52-53	34.16311147971311	38.0	33.0	40.0	22.5	41.0
54-55	33.93939445516167	38.0	33.0	40.0	22.0	41.0
56-57	33.66053251212796	37.5	33.0	40.0	21.0	41.0
58-59	33.41878805157007	37.0	32.5	40.0	20.0	41.0
60-61	33.1275654770355	37.0	32.0	40.0	19.5	41.0
62-63	32.80680372420404	36.0	32.0	39.5	18.5	41.0
64-65	32.4925965410811	36.0	32.0	39.0	18.0	41.0
66-67	32.158821775043	35.0	31.0	39.0	17.0	40.5
68-69	31.79197088903786	35.0	31.0	38.0	14.5	40.0
70-71	31.42892617545722	35.0	31.0	37.0	13.0	40.0
72-73	31.224171738275214	35.0	31.0	37.0	13.0	39.0
74-75	31.024131160249162	35.0	31.0	36.5	11.5	39.0
76-77	30.804200193180804	35.0	31.0	36.0	10.5	39.0
78-79	30.504938024705016	35.0	31.0	35.5	8.0	37.0
80-81	30.23191308952735	35.0	30.0	35.0	7.0	37.0
82-83	30.031573301747716	34.5	30.0	35.0	7.0	36.5
84-85	29.891194607029476	34.0	30.0	35.0	7.0	36.0
86-87	29.715878580024395	34.0	30.0	35.0	3.5	36.0
88-89	29.585163180528117	34.0	30.0	35.0	2.0	35.5
90-91	29.451369421343422	34.0	30.0	35.0	2.0	35.0
92-93	29.343151229931962	34.0	30.0	35.0	2.0	35.0
94-95	29.25671365181733	34.0	30.0	35.0	2.0	35.0
96-97	29.193629133862228	34.0	30.0	35.0	2.0	35.0
98-99	29.154817934636498	34.0	30.0	35.0	2.0	35.0
100-101	29.028002512820372	34.0	29.0	35.0	2.0	35.0
102-103	27.646566553571695	32.5	26.0	34.0	2.0	35.0
104-105	28.75085884997464	34.0	29.0	35.0	2.0	35.0
106-107	29.0432703335947	34.0	29.5	35.0	2.0	35.0
108-109	29.25711053508452	34.0	30.0	35.0	2.0	35.0
110-111	29.250354815961828	34.0	30.0	35.0	2.0	35.0
112-113	29.187117114991317	34.0	30.0	35.0	2.0	35.0
114-115	29.084537296389406	34.0	30.0	35.0	2.0	35.0
116-117	28.995268540270306	34.0	30.0	35.0	2.0	35.0
118-119	28.86402067506517	34.0	30.0	35.0	2.0	35.0
120-121	28.712774496420508	34.0	30.0	35.0	2.0	35.0
122-123	28.54285101443131	34.0	30.0	35.0	2.0	35.0
124-125	27.741195941578475	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	52202.0
3	17969.0
4	9379.0
5	7990.0
6	8212.0
7	9894.0
8	11735.0
9	12831.0
10	13733.0
11	14365.0
12	15029.0
13	15733.0
14	16527.0
15	14169.0
16	11321.0
17	10453.0
18	10130.0
19	9704.0
20	9553.0
21	9941.0
22	11261.0
23	14414.0
24	19560.0
25	26498.0
26	33840.0
27	40211.0
28	48498.0
29	57545.0
30	68128.0
31	82655.0
32	101388.0
33	128235.0
34	169941.0
35	245621.0
36	433313.0
37	640644.0
38	182333.0
39	188.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.461770965676514	18.922859069558225	13.525850809408007	25.089519155357255
2	17.111452377820928	19.70225983892555	37.50547359137223	25.68081419188129
3	19.427703386447405	23.62064857136668	28.690107414410427	28.26154062777549
4	14.279414061425195	15.902181322558807	34.22673244957094	35.59167216644506
5	15.082539944270202	36.54308028102559	33.23656676873604	15.137813005968177
6	34.700882078612196	34.43816571286428	16.276460714212487	14.584491494311036
7	30.056254867316895	30.736296169631967	20.25890934188266	18.948539621168482
8	27.31897163703608	35.00766958684815	18.646554875748826	19.02680390036694
9	26.936941154440337	15.430801712087488	18.46506396727488	39.1671931661973
10-11	26.38789424955962	25.123873703954324	26.633784567505025	21.85444747898103
12-13	26.770147368885333	22.34083980474624	26.970871919513634	23.91814090685479
14-15	24.06797997420745	23.79167542088949	23.955210107151686	28.185134497751374
16-17	24.405053946006014	26.83943421311265	24.71049776430912	24.04501407657222
18-19	24.405822593260602	26.048585065264575	26.143457669194476	23.40213467228035
20-21	25.211412758223823	25.064573488024667	25.956017479096683	23.767996274654827
22-23	25.00053204147215	24.681403892995817	25.88041255463099	24.437651510901045
24-25	24.588252751511106	25.106449415512472	25.910761833253463	24.394535999722958
26-27	24.39034157741596	25.344808239955423	25.89749195195939	24.367358230669225
28-29	24.40671427681495	25.368654919803458	26.011148157276775	24.21348264610482
30-31	24.649745654733508	25.256192834223068	25.86689619068292	24.227165320360506
32-33	24.478653603440122	25.22050191947714	25.767384597288977	24.53345987979376
34-35	24.542275271905098	25.26069401165527	25.886464108759334	24.3105666076803
36-37	24.666031328147703	25.18128622387209	25.84763715592763	24.30504529205258
38-39	24.577116911299225	25.323377771173757	25.8070320444657	24.29247327306132
40-41	24.628463373732103	25.23100121420739	25.880919454481155	24.259615957579356
42-43	24.575122682146592	25.130701346238858	25.800173926670272	24.494002044944278
44-45	24.542437348310216	25.190066271930633	25.73477104697269	24.532725332786466
46-47	24.659982560678255	25.158456184719835	25.71154618914835	24.470015065453563
48-49	24.601177513223472	25.4065125190824	25.544081876886356	24.44822809080777
50-51	24.499140211333113	25.624913905050235	25.51958785863867	24.356358024977983
52-53	24.742377338917763	25.420142827376907	25.430899474297902	24.40658035940743
54-55	24.553970118667863	25.31414015716101	25.584865243023796	24.547024481147332
56-57	24.533433471138895	25.52434503664916	25.444985438810015	24.497236053401927
58-59	24.520198444112722	25.385876449890233	25.566413852395776	24.52751125360127
60-61	24.5571667026505	25.487619626897956	25.640376801029525	24.31483686942202
62-63	24.422569883244815	25.57224512475323	25.53148102718561	24.473703964816345
64-65	24.41784468841563	25.382931234899058	25.573429463416446	24.625794613268862
66-67	24.429585075743827	25.44755781346073	25.46421607683	24.658641033965445
68-69	24.39805619754382	25.438541505847155	25.511672062463553	24.651730234145468
70-71	24.303449540844184	25.309873581717195	25.61584846987403	24.770828407564586
72-73	24.406011534387808	25.381419210332112	25.486388098907973	24.726181156372103
74-75	24.271432565059214	25.537938592885006	25.49564756444497	24.694981277610808
76-77	24.38892420404517	25.225570993201046	25.521895891263192	24.86360891149059
78-79	24.277973607739895	25.32249393374213	25.562764754940204	24.836767703577774
80-81	24.28423071148992	25.512828052863	25.478720769884372	24.724220465762713
82-83	24.34281126518057	25.318449804363492	25.55214958333535	24.78658934712059
84-85	24.209052460817027	25.43041253715856	25.417857891078675	24.942677110945734
86-87	24.18184195773732	25.437036389360784	25.500640490200364	24.880481162701535
88-89	24.048485411238076	25.313799113628495	25.557135368002804	25.08058010713063
90-91	24.02908001123551	25.366604995159907	25.559454195558256	25.04486079804632
92-93	24.111595093353806	25.355377439084787	25.549335751782888	24.983691715778527
94-95	24.103116762828662	25.189799516462447	25.607043135743744	25.100040584965143
96-97	24.026177777519845	25.125926871685945	25.680744089590984	25.167151261203223
98-99	23.96589946260896	25.200757531792732	25.53313524534667	25.300207760251638
100-101	23.93129953365411	25.201268479393597	25.66241060994452	25.205021377007775
102-103	23.74991222550279	25.100739975281925	25.746702242016973	25.402645557198312
104-105	23.757850755415465	25.250768608469016	25.572797808336624	25.41858282777889
106-107	23.73692276086676	25.16507469419543	25.634193626513163	25.463808918424647
108-109	23.64499878229741	25.21044783019713	25.654720672179565	25.489832715325893
110-111	23.572117332900042	25.294971599124523	25.623557106892324	25.509353961083114
112-113	23.56741196494169	25.298508429844404	25.65838786008012	25.475691745133794
114-115	23.46239334110787	25.45132188068982	25.603317995098134	25.482966783104178
116-117	23.36820050499951	25.340615477341366	25.61162620751712	25.679557810142
118-119	23.432732440972813	25.263165631631363	25.591209959394845	25.712891968000978
120-121	23.285087113594084	25.33878153537637	25.692930141624625	25.683201209404917
122-123	23.24173756016002	25.390824922891113	25.517402255567834	25.85003526138103
124-125	23.19443936458617	25.436292094366543	25.560937302550744	25.80833123849654
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	17.0
1	25.5
2	72.0
3	115.5
4	109.5
5	97.5
6	119.0
7	283.5
8	448.0
9	485.5
10	434.0
11	350.5
12	347.5
13	382.5
14	419.5
15	484.5
16	553.5
17	630.5
18	717.5
19	789.5
20	882.0
21	995.5
22	1138.0
23	1394.0
24	1718.0
25	2137.5
26	2824.0
27	3776.0
28	5100.5
29	7080.0
30	9695.5
31	12826.0
32	16624.0
33	20225.0
34	23846.0
35	28481.0
36	34172.5
37	40201.0
38	45803.0
39	51575.0
40	57977.0
41	64397.0
42	71083.5
43	78532.5
44	86022.0
45	93314.5
46	101238.5
47	107774.5
48	113494.0
49	118100.0
50	119312.5
51	120712.0
52	120655.0
53	119730.5
54	118750.0
55	113635.0
56	105063.5
57	95093.0
58	86575.5
59	77914.0
60	67287.0
61	56719.0
62	45952.0
63	34641.0
64	24924.5
65	18104.0
66	13396.5
67	9987.0
68	7334.0
69	5387.0
70	4044.0
71	3045.0
72	2192.0
73	1637.5
74	1238.5
75	902.0
76	615.0
77	375.0
78	227.5
79	130.5
80	81.0
81	54.5
82	42.0
83	24.5
84	12.5
85	6.5
86	4.5
87	5.5
88	5.0
89	4.0
90	2.0
91	1.5
92	2.0
93	2.5
94	2.0
95	2.5
96	3.5
97	2.5
98	2.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07338085359301207
2	1.1604773894519569E-4
3	5.802386947259784E-4
4	0.18320069721481558
5	0.06212422291532809
6	0.008742263000538073
7	0.011604773894519568
8	0.009980105549286829
9	0.017871351797560133
10-11	0.017561891160372947
12-13	0.01959272659191387
14-15	0.026149423842317426
16-17	0.027348583811417783
18-19	0.0241572709904249
20-21	0.026478225769328814
22-23	0.029321395373486107
24-25	0.027058464464054795
26-27	0.025646550306888245
28-29	0.02630415416091102
30-31	0.02568523288653664
32-33	0.022629309094313158
34-35	0.025046970322338068
36-37	0.0268457102759886
38-39	0.03322833591797436
40-41	0.02866379151946333
42-43	0.027870798636671162
44-45	0.02767738573842917
46-47	0.029147323765068314
48-49	0.024312001309018495
50-51	0.03044319018328967
52-53	0.0268650515658128
54-55	0.03044319018328967
56-57	0.027251877362296784
58-59	0.024640803236029882
60-61	0.025104994191810667
62-63	0.029437443112431304
64-65	0.03353779655516155
66-67	0.03249336690465479
68-69	0.02800618766544056
70-71	0.03651635518808824
72-73	0.0403846131529281
74-75	0.025936669654251232
76-77	0.01856763823123131
78-79	0.021642903313278995
80-81	0.025182359351107464
82-83	0.024389366468315292
84-85	0.017117041494416365
86-87	0.015627762177953017
88-89	0.017329795682482555
90-91	0.018606320810879706
92-93	0.021933022660641982
94-95	0.017097700204592163
96-97	0.01965075046138647
98-99	0.016749556987756577
100-101	0.01831620146351672
102-103	0.015260277671293234
104-105	0.017000993755471167
106-107	0.015627762177953017
108-109	0.013829022224302484
110-111	0.010657050693133803
112-113	0.009651303622275441
114-115	0.0080653178566911
116-117	0.007349690133195726
118-119	0.004893346325522418
120-121	0.002707780575387899
122-123	0.0062665779030405665
124-125	0.054523096014417766
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2585143.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.234458409947706
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.8313461628694	44.546349449489924
2	11.814313123417246	13.523716262086477
3	3.9440332077802474	6.772038137944538
4	1.8959579292313	4.340565009903977
5	1.053115259831516	3.0137240769854095
6	0.6994791774019347	2.402058713258128
7	0.4809732587043496	1.9269770780127784
8	0.36268640445185146	1.6606527945162375
9	0.2725989485242756	1.4041847863717314
>10	1.5552866945287642	15.878315872426493
>50	0.07174549003416802	2.7941041632115375
>100	0.018125052144219143	1.5193143761374908
>500	1.3571643229605922E-4	0.0471885132357778
>1k	2.035746484309504E-4	0.17081076641947446
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.736515929679712E-5
2	0.0	0.0	0.0	0.0	7.736515929679712E-5
3	0.0	0.0	0.0	0.0	7.736515929679712E-5
4	0.0	0.0	0.0	0.0	7.736515929679712E-5
5	0.0	0.0	0.0	0.0	7.736515929679712E-5
6	0.0	0.0	0.0	0.0	7.736515929679712E-5
7	0.0	0.0	0.0	0.0	7.736515929679712E-5
8	0.0	0.0	0.0	0.0	7.736515929679712E-5
9	0.0	0.0	0.0	3.868257964839856E-5	7.736515929679712E-5
10-11	0.0	0.0	0.0	3.868257964839856E-5	1.1604773894519568E-4
12-13	0.0	0.0	0.0	3.868257964839856E-5	2.3209547789039136E-4
14-15	0.0	0.0	0.0	3.868257964839856E-5	2.3209547789039136E-4
16-17	0.0	0.0	0.0	7.736515929679712E-5	3.094606371871885E-4
18-19	0.0	0.0	0.0	1.1604773894519568E-4	3.4814321683558706E-4
20-21	0.0	0.0	0.0	1.1604773894519568E-4	3.8682579648398563E-4
22-23	0.0	0.0	0.0	1.1604773894519568E-4	4.061670863081849E-4
24-25	0.0	0.0	0.0	2.1275418806619207E-4	4.83532245604982E-4
26-27	0.0	0.0	0.0	5.222148252533806E-4	5.028735354291813E-4
28-29	0.0	0.0	0.0	0.0027271218652120985	5.028735354291813E-4
30-31	0.0	0.0	0.0	0.01127597196750818	5.028735354291813E-4
32-33	0.0	0.0	0.0	0.02659427350827401	5.802386947259784E-4
34-35	0.0	0.0	0.0	0.044465625305834144	6.18921274374377E-4
36-37	0.0	0.0	0.0	0.07289732134740709	6.18921274374377E-4
38-39	0.0	0.0	0.0	0.12500275613379994	6.576038540227756E-4
40-41	0.0	0.0	0.0	0.18273650625903481	6.962864336711741E-4
42-43	0.0	0.0	0.0	0.2371435545345074	6.962864336711741E-4
44-45	0.0	0.0	0.0	0.29971262711579205	7.156277234953734E-4
46-47	0.0	0.0	0.0	0.3721264162175941	7.349690133195727E-4
48-49	0.0	0.0	0.0	0.4492401387466767	7.349690133195727E-4
50-51	0.0	0.0	0.0	0.5299319998932361	7.349690133195727E-4
52-53	0.0	0.0	0.0	0.6110106868362795	7.54310303143772E-4
54-55	0.0	0.0	0.0	0.7015859470830046	7.736515929679713E-4
56-57	0.0	0.0	0.0	0.806241666321747	7.929928827921705E-4
58-59	0.0	0.0	0.0	0.9213416820655569	8.123341726163698E-4
60-61	0.0	0.0	0.0	1.0418766002499669	8.123341726163698E-4
62-63	0.0	0.0	0.0	1.1675176189479655	8.896993319131669E-4
64-65	0.0	0.0	0.0	1.300856470995995	8.896993319131669E-4
66-67	0.0	3.868257964839856E-5	0.0	1.4532464935208613	9.090406217373661E-4
68-69	0.0	3.868257964839856E-5	0.0	1.6190980537633703	9.283819115615654E-4
70-71	0.0	3.868257964839856E-5	0.0	1.800500011024535	9.283819115615654E-4
72-73	0.0	3.868257964839856E-5	0.0	1.9867179494519258	0.001025088360682562
74-75	0.0	3.868257964839856E-5	0.0	2.1822583895745806	0.0010444296505067612
76-77	0.0	3.868257964839856E-5	0.0	2.398532692388777	0.0010444296505067612
78-79	0.0	3.868257964839856E-5	0.0	2.647029584050089	0.0010444296505067612
80-81	0.0	3.868257964839856E-5	0.0	2.9055259225505123	0.0010637709403309606
82-83	0.0	3.868257964839856E-5	0.0	3.1726484763125287	0.0011024535199793588
84-85	0.0	3.868257964839856E-5	0.0	3.459537828274877	0.0011217948098035582
86-87	0.0	3.868257964839856E-5	0.0	3.7598693766650433	0.0011217948098035582
88-89	0.0	3.868257964839856E-5	0.0	4.085479990855438	0.0012185012589245546
90-91	0.0	3.868257964839856E-5	0.0	4.435789432151336	0.0012765251283971524
92-93	0.0	5.802386947259784E-5	0.0	4.795750177069508	0.0014699380266391454
94-95	0.0	7.736515929679712E-5	0.0	5.167953958446399	0.0015473031859359423
96-97	0.0	7.736515929679712E-5	0.0	5.555108556857396	0.001779398663826334
98-99	0.0	7.736515929679712E-5	0.0	5.981932140697825	0.0018954464027715295
100-101	0.0	7.736515929679712E-5	0.0	6.437767659274554	0.0018954464027715295
102-103	0.0	7.736515929679712E-5	0.0	6.91131979933025	0.001953470272244127
104-105	0.0	7.736515929679712E-5	0.0	7.391331930187228	0.002011494141716725
106-107	0.0	7.736515929679712E-5	0.0	7.888673856726688	0.002011494141716725
108-109	0.0	7.736515929679712E-5	0.0	8.4131516128895	0.002011494141716725
110-111	0.0	7.736515929679712E-5	0.0	8.970451537883978	0.002011494141716725
112-113	0.0	7.736515929679712E-5	0.0	9.543959463751134	0.002011494141716725
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3240	0.0	47.144646	1
GTATCAA	5100	0.0	45.511044	1
TCAACGC	6750	0.0	33.84327	4
CAACGCA	6970	0.0	32.877327	5
TATCAAC	7075	0.0	32.455353	2
ATCAACG	7270	0.0	31.504002	3
AACGCAG	7355	0.0	31.381512	6
GTACATG	10045	0.0	27.442865	1
ACGCAGA	8570	0.0	26.446547	7
TACATGG	10345	0.0	26.001482	2
ACATGGG	10300	0.0	25.884466	3
CATGGGG	8035	0.0	25.164665	4
CGCAGAG	9075	0.0	25.04187	8
AGAGTAC	8805	0.0	22.567307	10-11
GCAGAGT	10320	0.0	21.329489	9
CAGAGTA	9745	0.0	19.413494	9
ACCGTGC	695	0.0	18.831629	8
GTATACG	255	1.4618216E-5	18.719196	1
GTACGTA	360	4.559297E-8	18.231718	1
CTAATAC	715	0.0	17.517904	3
>>END_MODULE